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Wild Caught Nematode Identification and Early Embryo Development: An accessible undergraduate research experience.

Haley Attix, Alex George, Henali Panchal, Angel Cortez, Martin Cho, Kathy Zarilla, Eric Hastie,

Ample evidence suggests that participation in undergraduate research in community college is critical for stimulating interest and retention in STEM careers. Guided skill development and practice in a collaborative lab setting allows students to be competitive when applying to future research opportunities. The goals of this undergraduate research experience (URE) ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans.

George Huang, Bailey de Jesus, Alex Koh, Sara Blanco, Aubrie Rettmann, Ella DeMott, Melynda Sylvester, Cassie Ren, Carrie Meng, Skye Waterland, Anita Rhodes, Persephone Alicea, Abbey Flynn, Daniel J Dickinson, Ryan Doonan,

Streamlined, selection-based CRISPR knock-in protocols for <i>C. elegans</i> were first introduced six years ago (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). Though these selection-based approaches are powerful, one drawback has been the requirement to inject large numbers of P0 worms (~30-60 per gene target). We have found that a ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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ATG8 is conserved between Saccharomyces cerevisiae and psychrophilic, polar-collected fungi.

Brenna J Ivory, Hannah M Smith, Elizabeth Cabrera, Meaghan R Robinson, Jackson T Sparks, Amanda Solem, Jun-Ichi Ishihara, Hiroki Takahashi, Masaharu Tsuji, Verónica A Segarra,

Autophagy is a conserved catabolic process by which eukaryotic cells respond to stress by targeting damaged or unneeded molecules or organelles for sequestration into specialized vesicles known as autophagosomes. Autophagosomes ultimately facilitate the digestion and recycling of their contents by fusing with the degradative organelle of the cell. Studies of ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Genetic mapping and phenotypic analysis of shotH.3.2 in Drosophila melanogaster.

Elyse M Talley, Charlie T Watts, Sonia Aboyer, Madeline G Adamson, Harriet Ab Akoto, Haley Altemus, Philip J Avella, Rebecca Bailey, Elizabeth R Bell, Katheryn L Bell, Kelsey Breneman, Jessica S Burkhart, Logan J Chanley, Savannah S Cook, Mackenzie T DesLaurier, Timothy R Dorsey, Cassandra J Doyle, Merris E Egloff, Ayoola S Fasawe, Katy K Garcia, Nathaniel P Graves, Tyler K Gray, Evan M Gustafson, Makayla J Hall, Jaden D Hayes, Lindsay J Holic, Brice A Jarvis, Piotr S Klos, Sidney Kritzmire, Lera Kuzovko, Edwyna Lainez, Shamerra McCoy, James C Mierendorf, Nicole A Neri, Caley R Neville, Kelley Osborn, Kaitlyn Parker, Megan E Parks, Kylee Peck, Robyn Pitt, Matthew E Platta, Brianna Powell, Katalina Rodriguez, Clara Ruiz, Mariah N Schaefer, Amanda B Shields, Jasmine B Smiley, Briona Stauffer, Devan Straub, John L Sweeney, Kaitlyn M Termine, Brett Thomas, Sophia D Toth, Taylor R Veile, Kayla S Walker, Paige N Webster, Brian J Woodard, Quentin L Yoder, McKenzie K Young, McKenzie L Zeedyk, Logan N Ziegler, Kayla L Bieser, David P Puthoff, Joyce Stamm, Alysia D Vrailas-Mortimer, Jacob D Kagey, Julie A Merkle,

Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in <i>Drosophila melanogaster</i> identified growth control phenotypes in the developing eye. One mutant line from this screen, <i>H.3.2</i>, was phenotypically characterized using the FLP/FRT system and genetically mapped by complementation analysis and genomic sequencing ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Annotating Putative D. discoideum Proteins Using I-TASSER.

Jacquelyn McCullough, Petra Fey, Ryan J Rahman, Morgan Wallace, Seeta Morey, Kyle Sahlberg, Ethan McGonagle, Danielle Hess, Chance Hatfield, Michaela-Romina Sarmiento, Jordi Velasquez, Richard H Gomer,

Using Gene Ontology annotation in any aspect or using any evidence code, we found that approximately 14% percent of predicted <i>D. discoideum</i> proteins have no GO annotations and no obvious similarity to any annotated protein across diverse organisms. We have been systematically examining these unannotated protein sequences using software that ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae.

Kelsey A Woodruff, Kyle A Richards, Melissa D Evans, Abigail R Scott, Brian M Voas, Courtney Broshar Irelan, James B Olesen, Philip J Smaldino, Eric M Rubenstein,

The heterotrimeric Asi ubiquitin ligase (encoded by <i>ASI1</i>, <i>ASI2</i>, and <i>ASI3</i>) mediates protein degradation in the inner nuclear membrane in <i>Saccharomyces cerevisiae</i>. Asi1p and Asi3p possess catalytic domains, while Asi2p functions as an adaptor for a subset of Asi substrates. We hypothesized the Asi complex is an important mediator of ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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YML018C protein localizes to the vacuolar membrane independently of Atg27p.

Candyce M Sturgeon, Nicholas Zanghi, Hannah M Smith, Emily K Davis, Meaghan R Robinson, Elizabeth Cabrera, Molly C Holbrook, Verónica A Segarra,

The function of the budding yeast YML018C protein remains to be determined. High-throughput studies have reported that the YML018C protein localizes to the vacuolar membrane and physically interacts with the autophagy-related protein Atg27p. While this evidence suggests a potential role for this uncharacterized protein in the process of autophagy, the ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Affordable Caenorhabditis elegans tracking system for classroom use.

Nicholas Leonard, Andrés G Vidal-Gadea,

For decades the nematode <i>C. elegans</i> has served as an outstanding research organism owing to its unsurpassed experimental amenability. This advantage has also made this tiny worm an attractive vehicle for science instruction across higher learning institutions. However, the prohibitive cost associated with the automated behavioral assessment of these animals ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

Cited: 1 time

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Genetic Mapping of a new Hippo allele, HpoN.1.2, in Drosophila melanogaster.

Jamie L Siders, Kayla L Bieser, Danielle R Hamill, Erika C Acosta, Olivia K Alexander, Humza I Ali, Micah J Anderson, Hayden R Arrasmith, Mustafa Azam, Nikki J Beeman, Hassan Beydoun, Lauren J Bishop, Morgan D Blair, Brianna Bletch, Heather R Bline, Jennifer C Brown, Kelly M Burns, Karina C Calagua, Lexie Chafin, William Ah Christy, Carlyn Ciamacco, Hannah Cizauskas, Caitlyn M Colwell, Abigail R Courtright, Lucero Diaz Alavez, Rayne Is Ecret, Fatima Edriss, Taylor G Ellerbrock, Madison M Ellis, Erica M Extine, Eric Feldman, Luke J Fickenworth, Caroline M Goeller, Alexis S Grogg, Yailine Hernandez, Abigail Hershner, Megan M Jauss, Leyre Jimenez Garcia, Katey E Franks, Ethan T Kazubski, Emily R Landis, Jon Langub, Tia N Lassek, Triet C Le, Julia M Lee, Daniel P Levine, Phoebe J Lightfoot, Natasha Love, Ali Maalhagh-Fard, Colin Maguire, Brynna E McGinnis, Bhargavi V Mehta, Veronica Melendrez, Zimri E Mena, Seth Mendell, Petra Montiel-Garcia, Autumn S Murry, Riley A Newland, Ryan M Nobles, Neha Patel, Yashodhara Patil, Cassidy L Pfister, Victoria Ramage, Mya R Ray, Joseph Rodrigues, Victoria C Rodriquez, Yara Romero, Alexandra M Scott, Nicholas Shaba, Samantha Sieg, Kayla Silva, Sahiba Singh, Aleksandria J Spargo, Savanna J Spitnale, Nicole Sweeden, Logan Tague, Breanna M Tavernini, Kathleen Tran, Liselle Tungol, Kylie A Vestal, Amber Wetherbee, Kayla M Wright, Anthony T Yeager, Rehab Zahid, Jacob D Kagey,

Genetic screens provide a mechanism to identify genes involved with different cellular and organismal processes. Using a Flp/FRT screen in the <i>Drosophila</i> eye we identified mutations that result in alterations and de-regulation of cell growth and division. From this screen a group of undergraduate researchers part of the Fly-CURE consortium ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Differential effects on neuromuscular physiology between Sod1 loss-of-function mutation and paraquat-induced oxidative stress in Drosophila.

Atsushi Ueda, Atulya Iyengar, Chun-Fang Wu,

Oxidative stress is thought to be a major contributor to aging processes. Here, we report differential effects on neurotransmission caused by loss-of-function mutations of <i>Superoxide dismutase 1</i> (<i>Sod1</i>) and by paraquat (PQ) feeding in <i>Drosophila</i>. We demonstrated alterations in <i>Sod1</i> mutants; the larval neuromuscular junction displayed supernumerary discharges and the ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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B.2.16 is a non-lethal modifier of the Dark82 mosaic eye phenotype in Drosophila melanogaster.

Alysia D Vrailas-Mortimer, Nitish Aggarwal, Neha N Ahmed, Ian M Alberts, Masa Alhawasli, Ibrahim A Aljerdi, Brooke M Allen, Ahmad M Alnajar, Michael A Anderson, Ryan Armstong, Chace C Avery, Eric J Avila, Tyra N Baker, Soolmaz Basardeh, Natasha A Bates, Farrah N Beidas, Ashton C Bosler, Deja M Brewer, Ryan S Buenaventura, Natalie Jl Burrell, Alyn P Cabrera-Lopez, Amairani B Cervantes-Gonzalez, Raymond P Cezar, Joselyn Coronel, Corinne Croslyn, Konnor R Damery, Lucero Diaz-Alavez, Nupur P Dixit, Dilcia L Duarte, Amanda R Emke, Katherine English, Audrey A Eshun, Samuel R Esterly, Arnold J Estrada, Mark Feng, Meghan M Freund, Niko Garcia, Charandeep S Ghotra, Haddia Ghyasi, Christa Sa Hale, Luci Hulsman, Lindsey Jamerson, Austin K Jones, Madeline Kuczynski, Takailah N Lacey-Kennedy, Mindy J Lee, Tala Mahjoub, Molly C Mersinger, Alyssa D Muckerheide, David W Myers, Karen Nielsen, Paul J Nosowicz, Jacinda A Nunez, Amy C Ortiz, Tulsi T Patel, Natalie N Perry, W Storm A Poser, Deisy M Puga, Cathrine Quam, Paulina Quintana-Lopez, Piper Rennerfeldt, Nicholas M Reyes, Ian G Rines, Cally Roberts, Daniel B Robinson, Kalina M Rossa, Gloria J Ruhlmann, Jeremy Schmidt, John R Sherwood, Dimyana H Shonoda, Hannah Soellner, Juan C Torrez, Mayukah Velide, Zachary Weinzapfel, Alisa C Ward, Kayla L Bieser, Julie A Merkle, Joyce C Stamm, Richard L Tillett, Jacob D Kagey,

Genetic screens have been used to identify genes involved in the regulation of different biological processes. We identified growth mutants in a Flp/FRT screen using the <i>Drosophila melanogaster</i> eye to identify conditional regulators of cell growth and cell division. One mutant identified from this screen, <i>B.2.16</i>, was mapped and characterized ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Genetic interactions in a C. elegans sod-1 ALS model: glutamatergic neuron degeneration.

Jennifer F Osborne, Katherine S Yanagi, Anne C Hart,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Glutamatergic transmission regulates locomotory behavior on a food patch in C. elegans.

Trevor Wolf, Ariana Perez, Gareth Harris,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Worms avoid a cat sensed repellent.

Brianna Ramos, Gareth Harris,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Variation between nematodes in a multi-sensory behavioral assay.

Renae Ellis, Gareth Harris,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Individual point mutations in two ERAD E2 ubiquitin-conjugating enzymes do not affect Caenorhabditis elegans spontaneous reversal frequency.

Mackenzi Oswald, Heino Hulsey-Vincent, Caroline Lina Dahlberg,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Mutations in two ERAD E3 ubiquitin ligase enzymes reduce spontaneous reversal frequency in Caenorhabditis elegans.

Mackenzi Oswald, Heino Hulsey-Vincent, Caroline Lina Dahlberg,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Phenotypic modulation of pentylenetetrazole-induced convulsive behaviors in C. elegans carrying a mutation associated with Alzheimer's disease.

Madeline A Vaji, Guy A Caldwell, Kim A Caldwell,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Enhanced pentylenetetrazole sensitivity in a C. elegans mutant associated with DNM1 encephalopathy.

Madeline A Vaji, Guy A Caldwell, Kim A Caldwell,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Expression of a FRET-based ATP Biosensor in the C. elegans Intestine.

Jose Soto, Madeline Rivera, Gina Broitman-Maduro, Morris F Maduro,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The plant terpenoid carvone is a chemotaxis repellent for C. elegans.

Clayton Ellington, Andrew Hayden, Zack LaGrange, Marina Luccioni, Mohammed Osman, Lauren Ramlan, Miranda Vogt, Sujay Guha, Miriam Goodman, Lauren O'Connell,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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fs(1)A13041 is a 5' UTR deletion of the essential gene small ovary in Drosophila.

Myles Hammond, Jillian G Gomez, Brian Oliver, Steve Kucera, Leif Benner,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Drosophila Plc21C is involved in calcium wave propagation during egg activation.

Qinan Hu, Adriana N Vélez-Avilés, Mariana F Wolfner,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

Cited: 1 time

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Generation of sas-6::ha by CRISPR/Cas9 editing.

Mary Bergwell, Amy Smith, Holly Lakin, Rebecca Slay, Jyoti Iyer,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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Double mutants of TTLL glutamylase genes have little to no difference in viability or brood size.

Pooja Shah, Monica Martinez, Monica Chakraborty, Sharwani Kota, Bhumi Shah, Jessica Lee, Nina Peel,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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