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Changes in Histone H3 Acetylation on Lysine 9 Accompany Aβ 1-40 Overexpression in an Alzheimer's Disease Yeast Model.

Muna M Hugais, Samantha N Cobos, Seth A Bennett, Jailene Paredes, Genevieve Foran, Mariana P Torrente,

Alzheimer's Disease (AD), the most common type of dementia, is a neurodegenerative disease characterized by plaques of amyloid-beta (Aβ) peptides found in the cerebral cortex of the brain. The pathological mechanism by which Aβ aggregation leads to neurodegeneration remains unknown. Interestingly, genetic mutations do not explain most AD cases suggesting ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Temperature stressed Caenorhabditis elegans males fail to mate successfully and successful males produce very few viable cross progeny.

Nicholas B Sepulveda, Lisa N Petrella,

Exposure to moderate temperature stress can have profoundly negative effects on an organism's reproductive capacity at temperatures where there are minimal or indiscernible effects on the organism as a whole. These negative effects are often more pronounced in males of the species that produce sperm. Previously we showed that few ... Read more >>

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[2021, 2021:]

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Microscopy analysis of the smallest subunit of the RPA complex, Rfa3p, prompts consideration of how RPA subunits gather at single-stranded DNA sites.

Agnès Ramonatxo, María Moriel-Carretero,

The heterotrimeric Replication Protein A (RPA) complex preserves genome integrity by protecting the single-stranded DNA that becomes exposed during repair, replication, and recombination. Its two biggest subunits, Rfa1p and Rfa2p (as named in <i>S. cerevisiae</i>) contact DNA and interact with other partners, while the smallest Rfa3p subunit is considered to ... Read more >>

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[2021, 2021:]

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High confidence ATFS-1 target genes for quantifying activation of the mitochondrial unfolded protein response.

Sonja K Soo, Jeremy M Van Raamsdonk,

The mitochondrial unfolded protein response (mitoUPR) is an evolutionarily conserved pathway that restores homeostasis to the mitochondria after various disturbances. This pathway has roles in both resistance to exogenous stressors and longevity. The mitoUPR is mediated by the transcription factor ATFS-1/ATF-5, which modulates the expression of genes involved in protein ... Read more >>

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[2021, 2021:]

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The Argonaute Proteins ALG-1 and ALG-2 Are Linked to Stress Resistance and Proteostasis.

Fabian Finger, Franziska Ottens, Thorsten Hoppe,

The conserved Argonaute-family members ALG-1 and ALG-2 are known to regulate processing and maturation of microRNAs to target mRNAs for degradation or translational inhibition (Bouasker and Simard 2012; Meister 2013). Consequently, depletion of <i>alg-1</i> and <i>alg-2</i> results in multiple phenotypes. Our data describe a role of microRNA-regulation in stress resistance ... Read more >>

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[2021, 2021:]

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Beta-blockers reduce intestinal permeability and early mortality following traumatic brain injury in Drosophila.

Amanda R Scharenbrock, Rebeccah J Katzenberger, Megan C Fischer, Barry Ganetzky, David A Wassarman,

Traumatic brain injury (TBI) frequently leads to non-neurological consequences such as intestinal permeability. The beta-blocker drug labetalol, which inhibits binding of catecholamine neurotransmitters to adrenergic receptors, reduces intestinal permeability in a rat TBI model. Using a <i>Drosophila melanogaster</i> TBI model, we previously found a strong positive correlation between intestinal permeability ... Read more >>

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[2021, 2021:]

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Decreased Mitochondrial Membrane Potential Activates the Mitochondrial Unfolded Protein Response.

Brandon J Berry, Tyrone O Nieves, Andrew P Wojtovich,

Mitochondria are ATP-producing organelles that also signal throughout the cell. Mitochondrial protein homeostasis is regulated through membrane potential-dependent protein import and quality control signaling. The mitochondrial unfolded protein response (UPR<sup>mt</sup>) is a specific program that responds to imbalances in nuclear and mitochondrial gene expression. Mounting evidence suggests that the electrochemical ... Read more >>

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[2021, 2021:]

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A single copy transgenic mutant FUS strain reproduces age-dependent ALS phenotypes in C. elegans.

Audrey Labarre, Gilles Tossing, Claudia Maios, James J Doyle, J Alex Parker,

Mutations in the human DNA/RNA binding protein FUS are associated with amyotrophic lateral sclerosis and frontotemporal lobar degeneration, including some aggressive and juvenile onset forms. Cytoplasmic inclusions of human FUS proteins are observed in various neurodegenerative disorders, such as Huntington's disease or spinocerebellar ataxia, suggesting that FUS proteinopathy may be ... Read more >>

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[2021, 2021:]

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Drosophila insulin receptor regulates diabetes-induced mechanical nociceptive hypersensitivity.

Harika Dabbara, Arielle Schultz, Seol Hee Im,

Painful diabetic neuropathy (PDN) is one of the predominant complications of diabetes that causes numbness, tingling, and extreme pain sensitivity. Understanding the mechanisms of PDN pathogenesis is important for patient treatments. Here we report <i>Drosophila</i> models of diabetes-induced mechanical nociceptive hypersensitivity. Type 2 diabetes-like conditions and loss of insulin receptor ... Read more >>

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[2021, 2021:]

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Inheritance of pheromone profiles from aged D. melanogaster.

Samuel G Brown, Dova B Brenman-Suttner, Abigail G McInnes, Katlynn Lew, Amanda J Moehring, Johannes H Bauer, Anne F Simon,

Through aging, <i>D. melanogaster</i> males and females change their social spacing. Flies are initially more social, but reduce sociability as they grow older. This preferred social space is inherited in their progeny. Here, we report that in females, the profiles of cuticular hydrocarbons (CHC), which are known to promote social ... Read more >>

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[2021, 2021:]

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Modeling human glucose-6-phosphate dehydrogenase mutations using C. elegans GSPD-1.

Luiza N Loges, Katherine M Walstrom,

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked, recessive condition that causes intermittent jaundice or hemolytic anemia because of low NADPH levels in red blood cells. We performed steady-state enzyme kinetics with the recombinant <i>C. elegans</i> ortholog of human G6PD, GSPD-1, and two mutants containing amino acid changes found in human ... Read more >>

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[2021, 2021:]

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Two plasmid modules for introducing the auxin-inducible degron into the fission yeast Schizosaccharomyces pombe by PCR-based gene targeting.

Xiuyi Song, Ruoming Xu, Tomoyasu Sugiyama,

Targeted protein degradation is a powerful approach to study and inhibit protein function <i>in vivo</i>. Introduction of the auxin-inducible degron (AID) system to the fission yeast <i>Schizosaccharomyces pombe</i> was previously reported, but, to the best of our knowledge, no plasmid for constructing AID-tagged fission yeast strains has been described so ... Read more >>

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[2021, 2021:]

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Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans.

George Huang, Bailey de Jesus, Alex Koh, Sara Blanco, Aubrie Rettmann, Ella DeMott, Melynda Sylvester, Cassie Ren, Carrie Meng, Skye Waterland, Anita Rhodes, Persephone Alicea, Abbey Flynn, Daniel J Dickinson, Ryan Doonan,

Streamlined, selection-based CRISPR knock-in protocols for <i>C. elegans</i> were first introduced six years ago (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). Though these selection-based approaches are powerful, one drawback has been the requirement to inject large numbers of P0 worms (~30-60 per gene target). We have found that a ... Read more >>

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[2021, 2021:]

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Deletion of a putative HDA-1 binding site in the hlh-2 promoter eliminates expression in C. elegans dorsal uterine cells.

Taylor N Medwig-Kinney, Nicholas J Palmisano, David Q Matus,

The helix-loop-helix transcription factor <i>hlh-2</i> (E/Daughterless) has been shown to play an important role in regulating cell fate patterning, cell cycle, and basement membrane invasion in the context of the development of the <i>C. elegans</i> somatic gonad. Here, using CRISPR/Cas9 genome engineering, we generated a new <i>hlh-2</i> allele (<i>hlh-2(Δ-1303-702)</i>) in ... Read more >>

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[2021, 2021:]

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The primordial germ line is refractory to perturbations of actomyosin regulator function in C. elegans L1 larvae.

Jack Bauer, Léa Lacroix, Jean-Claude Labbé,

Cytokinesis, the separation of daughter cells at the end of mitosis, relies on the coordinated activity of several regulators of actomyosin assembly and contractility (Green et al. 2012). These include the small GTPase RhoA (RHO-1) and its guanine-nucleotide exchange factor Ect2 (ECT-2), the scaffold protein Anillin (ANI-1), the non-muscle myosin ... Read more >>

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[2021, 2021:]

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Direct comparisons of bisulfite pyrosequencing versus targeted bisulfite sequencing.

Dillon E King, A Clare Sparling, Rashmi Joglekar, Joel N Meyer, Susan K Murphy,

DNA methylation is an important epigenetic mechanism involved in proper genome function. Bisulfite pyrosequencing (PSQ) is a commonly used technique to quantify DNA methylation. Although very accurate, bisulfite pyrosequencing can be expensive and time consuming for large-scale quantitative DNA methylation analysis at the single nucleotide level. High throughput DNA methylation ... Read more >>

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[2021, 2021:]

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GPN-1/glypican and UNC-52/perlecan do not appear to function in BMP signaling to pattern the C. elegans postembryonic mesoderm.

Melisa S DeGroot, Robert Greer, Jun Liu,

Heparan sulfate proteoglycans (HSPGs) are diverse macromolecules consisting of a protein core modified with glycosaminoglycan (GAG) chains. HSPGs, including glypicans and perlecans, have been implicated in shaping the extracellular matrix (ECM) to affect growth factor signaling. Here, we tested if GPN-1/glypicanor UNC-52/perlecan plays a role in the bone morphogenetic protein ... Read more >>

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[2021, 2021:]

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Efficient generation of a single-copy eft-3p::TIR1::F2A:: BFP::AID*::NLS allele in the C. elegans ttTi5605 insertion site through recombination-mediated cassette exchange.

An A Vo, Max T Levenson, James Matthew Ragle, Jordan D Ward,

The auxin-inducible degron (AID) system is a widely used system to conditionally deplete proteins. Using CRISPR/Cas9-based genome editing in <i>C. elegans</i>, we recently generated a set of single-copy, tissue-specific and pan-somatic TIR1-expressing strains carrying a BFP reporter inserted in single-copy into two commonly used, well-characterized genetic loci. However, we were ... Read more >>

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[2021, 2021:]

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DMSO reduces the cytotoxicity of anticancer ruthenium complex KP1019 in yeast.

Jonathan Davis, Anne Cetto, Mary Campbell, Seth Scoggins, Laura Stultz, Pamela Hanson,

Low solubility in aqueous solutions is a significant limitation of the otherwise promising anticancer ruthenium complex KP1019. In laboratory studies, this challenge is often overcome by using DMSO to help drive the drug into solution. Since DMSO was previously shown to alter the bioactivity of platinum-based chemotherapeutics, here we examine ... Read more >>

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[2021, 2021:]

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Synergistic effects of hmp-2/β-catenin and sma-1H-spectrin on epidermal morphogenesis in Caenorhabditis elegans.

Sydney Wieberg, Harper Euwer, Anna Gerst, Stephanie L Maiden,

The F-actin, spectrin, and microtubule cytoskeletons are important mediators of embryonic epidermal morphogenesis in <i>Caenorhabditis elegans</i>. While SMA-1/β<sub>H</sub>-spectrin is known to organize actin bundles that connect to cadherin-based adhesions, the role of microtubules in the developing epidermis is not well understood. To determine if the spectrin cytoskeleton also plays a ... Read more >>

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[2021, 2021:]

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Swimming exercise reduces native ⍺-synuclein protein species in a transgenic C. elegans model of Parkinson's disease.

Minna Y Schmidt, Manish Chamoli, Gordon J Lithgow, Julie K Andersen,

Exercise has been historically recommended to prevent many disease conditions. Intense exercise in particular, has been shown to be beneficial for Parkinson's disease (PD) - stopping and even reversing symptoms in some patients. Recent research in mammalian animal models of Parkinson's have shown that exercise affects ⍺-synuclein aggregate species, considered ... Read more >>

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[2021, 2021:]

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Acute exposure of Nicotine during Drosophila puncture injury activates an epidermal wound response reaction.

Chrissy Cherenfant, Michelle T Juarez,

Epidermal wound reaction after injury can be visualized in <i>Drosophila melanogaster</i> embryos by transgenic fluorescent wound reporters. A "local" reaction is limited to the epidermal cells surrounding a wound site occurs in <i>wildtype</i> embryos. A "global" reaction extends beyond the wound site to all epidermal cells occurs in immune response ... Read more >>

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[2021, 2021:]

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Identification and bioinformatic analysis of neprilysin and neprilysin-like metalloendopeptidases in Drosophila melanogaster.

Heiko Meyer, Annika Buhr, Patrick Callaerts, Ronja Schiemann, Mariana F Wolfner, Steven J Marygold,

The neprilysin (M13) family of metalloendopeptidases comprises highly conserved ectoenzymes that cleave and thereby inactivate many physiologically relevant peptides in the extracellular space. Impaired neprilysin activity is associated with numerous human diseases. Here, we present a comprehensive list and classification of M13 family members in <i>Drosophila melanogaster</i>. Seven <i>Neprilysin</i> (<i>Nep</i>) ... Read more >>

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[2021, 2021:]

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Effect of the protease plasmin on C. elegans hyperactive DEG/ENaC channels MEC-4(d) and UNC-8(d).

Christina K Johnson, David D Miller, Laura Bianchi,

<i>C. elegans</i> MEC-4 and UNC-8 belong to the DEG/ENaC family of voltage-independent Na<sup>+</sup> channels and have been implicated in mechanosensation and synaptic remodeling. MEC-4 and UNC-8 hyperactive mutants, designated (d) mutants, conduct enhanced currents and cause cell death due to uncontrolled influx of cations. We show here that MEC-4(d) but ... Read more >>

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[2021, 2021:]

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The Joubert syndrome protein CEP41 is excluded from the distal segment of cilia in C. elegans.

Sebiha Cevik, Oktay I Kaplan,

Rare diseases are a fundamental issue in today's world, affecting more than 300 million individuals worldwide. According to data from Orphanet and OMIM, about 50-60 new conditions are added to the list of over 6,000 clinically distinct diseases each year, rendering disease diagnosis and treatment even more challenging. Ciliopathies comprise ... Read more >>

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[2021, 2021:]

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