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Highly improved cloning efficiency for plasmid-based CRISPR knock-in in C. elegans.

Ella DeMott, Daniel J Dickinson, Ryan Doonan,

Plasmid-based CRISPR knock-in is a streamlined, scalable, and versatile approach for generating fluorescent protein tags in <i>C. elegans</i> (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). However, compared to more recent protocols that utilize commercially available Cas9/RNP products and linear DNA repair templates (Dokshin <i>et al</i>. 2018; Ghanta and Mello ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Differential effects on neuromuscular physiology between Sod1 loss-of-function mutation and paraquat-induced oxidative stress in Drosophila.

Atsushi Ueda, Atulya Iyengar, Chun-Fang Wu,

Oxidative stress is thought to be a major contributor to aging processes. Here, we report differential effects on neurotransmission caused by loss-of-function mutations of <i>Superoxide dismutase 1</i> (<i>Sod1</i>) and by paraquat (PQ) feeding in <i>Drosophila</i>. We demonstrated alterations in <i>Sod1</i> mutants; the larval neuromuscular junction displayed supernumerary discharges and the ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Genetic interactions in a C. elegans sod-1 ALS model: glutamatergic neuron degeneration.

Jennifer F Osborne, Katherine S Yanagi, Anne C Hart,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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The pharmacological properties and corresponding mechanisms of farrerol: a comprehensive review.

Xiaojiang Qin, Xinrong Xu, Xiaomin Hou, Ruifeng Liang, Liangjing Chen, Yuxuan Hao, Anqi Gao, Xufeng Du, Liangyuan Zhao, Yiwei Shi, Qingshan Li,

<h4>Context</h4>Farrerol, a typical natural flavanone isolated from the traditional Chinese herb 'Man-shan-hong' [<i>Rhododendron dauricum</i> L. (Ericaceae)] with phlegm-reducing and cough-relieving properties, is widely used in China for treating bronchitis and asthma.<h4>Objective</h4>To present the anti-inflammatory, antioxidant, vasoactive, antitumor, and antimicrobial effects of farrerol and its underlying molecular mechanisms.<h4>Methods</h4>The literature was reviewed ... Read more >>

Pharm Biol (Pharmaceutical biology)
[2022, 60(1):9-16]

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Silencing the ASI gustatory neuron pair increases expression of the stress-resistance gene sod-3 in a daf-16 and daf-3 independent manner.

Peter Chisnell, Cynthia Kenyon,

MicroPubl Biol (microPublication biology)
[2018, 2018:]

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Inhibition of microRNA-29b suppresses oxidative stress and reduces apoptosis in ischemic stroke.

Yao-Hua Ma, Wen-Jing Deng, Zhi-Yi Luo, Jing Jing, Peng-Wei Pan, Yao-Bing Yao, Yan-Bo Fang, Jun-Fang Teng,

MicroRNAs (miRNAs) regulate protein expression by antagonizing the translation of mRNAs and are effective regulators of normal nervous system development, function, and disease. MicroRNA-29b (miR-29b) plays a broad and critical role in brain homeostasis. In this study, we tested the function of miR-29b in animal and cell models by inhibiting ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(2):433-439]

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Systemic epigallocatechin gallate protects against retinal degeneration and hepatic oxidative stress in the P23H-1 rat.

Lorena Perdices, Lorena Fuentes-Broto, Francisco Segura, Ana Cavero, Elvira Orduna-Hospital, Gema Insa-Sánchez, Ana Isabel Sánchez-Cano, Laura Fernández-Sánchez, Nicolás Cuenca, Isabel Pinilla,

Retinitis pigmentosa (RP) is a group of inherited retinal disorders that lead to photoreceptor loss. RP has been reported to be related to oxidative stress, autophagy, and inflammation. (-)-Epigallocatechin gallate (EGCG), the most abundant catechin-based flavonoid in green tea leaves, has significant antioxidant, anti-carcinogenic, antimicrobial, and neuroprotective properties. EGCG, given ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(3):625-631]

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A Systematic Review and Meta-Analysis of the Effect of Statins on Glutathione Peroxidase, Superoxide Dismutase, and Catalase.

Angelo Zinellu, Arduino A Mangoni,

Statins may exert protective effects against oxidative stress by upregulating specific antioxidant mechanisms. We conducted a systematic review and meta-analysis of the effect of statins on three key antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase. The electronic databases PubMed, Web of Science, and Scopus were searched from ... Read more >>

Antioxidants (Basel) (Antioxidants (Basel, Switzerland))
[2021, 10(11):]

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A New Strategy for Improving the Surface Quality of Ti6Al4V Machined by Abrasive Water Jet: Reverse Cutting with Variable Standoff Distances

Jie Xiong, Liang Wan, Yi'nan Qian, Shuo Sun, Deng Li, Shijing Wu,

Titanium alloys are widely used in important structures of aerospace vehicles, but the low thermal conductivity and high chemical activity make them difficult to process. As an untraditional machining technology, abrasive water jet (AWJ) has been proven to be an effective method for this kind of material. Aimed at further ... Read more >>

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Post-Operative Outcomes Associated with Open versus Robotic Thymectomy: A Propensity Matched Analysis.

Stephan A Soder, Clare Pollock, Pasquale Ferraro, Edwin Lafontaine, Jocelyne Martin, Basil Nasir, Moishe Liberman,

<h4>Objective</h4>To compare post-operative outcomes associated with thymectomy performed using either open or robotic approaches.<h4>Methods</h4>Retrospective cohort study from a single-center prospective registry consisting of patients undergoing thymectomy between 2000 and 2020. Patients were grouped according to surgical approach (open vs robotic). A propensity-score matching analysis was performed in a 2:1 open ... Read more >>

Semin Thorac Cardiovasc Surg (Seminars in thoracic and cardiovascular surgery)
[2021, :]

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Comparison of the antioxidant activities of nonfumigated and sulphur-fumigated Chrysanthemum morifolium cv. Hang-ju induced by oxidative stress.

Hongyan Ma, Shanshan Liu, Wenshan Qu, Qi Huang, Linyuan Li, Fujiang Chu, Yuyun Zhu, Xinlin Lv, Zhimin Wang, Jingjing Zhu,

<h4>Context</h4>The traditional drying method, sun drying, for <i>Chrysanthemum morifolium</i> Ramat. cv. Hang-ju (Compositae) (HJ) is widely replaced by sulphur fumigation (SF), which has an unknown effect on its efficacy.<h4>Objective</h4>To investigate protective effects of nonfumigated HJ (NHJ) and sulphur-fumigated HJ (SHJ) water extracts against oxidative stress and lipid peroxidation.<h4>Materials and methods</h4>Sprague-Dawley ... Read more >>

Pharm Biol (Pharmaceutical biology)
[2021, 59(1):40-46]

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Differences in Cadmium Accumulation, Detoxification and Antioxidant Defenses between Contrasting Maize Cultivars Implicate a Role of Superoxide Dismutase in Cd Tolerance.

Aya Mahmoud, Hamada AbdElgawad, Badreldin A Hamed, Gerrit T S Beemster, Nadia M El-Shafey,

Cadmium (Cd), a readily absorbed and translocated toxic heavy metal, inhibits plant growth, interrupts metabolic homeostasis and induces oxidative damage. Responses towards Cd-stress differ among plant cultivars, and the complex integrated relationships between Cd accumulation, detoxification mechanisms and antioxidant defenses still need to be unraveled. To this end, 12 Egyptian ... Read more >>

Antioxidants (Basel) (Antioxidants (Basel, Switzerland))
[2021, 10(11):]

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Elevated activity of superoxide dismutase in male late-life schizophrenia and its correlation with clinical symptoms and cognitive deficits.

Lijuan Huo, Xiaobing Lu, Fengchun Wu, Catherine Chang, Yuping Ning, Xiang Yang Zhang,

Despite inconsistent findings, accumulative evidence has shown abnormalities of the key antioxidant enzyme, superoxide dismutase (SOD), in patients with schizophrenia. However, few studies explored SOD in late-life schizophrenia (LLS). Our work aimed to investigate changes in SOD activity and the relationship between SOD activity and psychotic symptoms or cognitive deficits ... Read more >>

BMC Psychiatry (BMC psychiatry)
[2021, 21(1):606]

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SIRT 3 was involved in Lycium barbarum seed oil protection testis from oxidative stress: in vitro and in vivo analyses.

Zhang-Jie Yang, Yu-Xin Wang, Shuai Zhao, Na Hu, Dong-Mei Chen, Hui-Ming Ma,

<h4>Context</h4><i>Lycium barbarum</i> L. (Solanaceae) seed oil (LBSO) exerts LBSO exerts protective effects in the testis <i>in vivo</i> and <i>in vitro</i> via upregulating SIRT3.<h4>Objective</h4>This study evaluates the effects and mechanism of LBSO in the d-galactose (d-gal)-induced ageing testis.<h4>Materials and methods</h4>Male Sprague Dawley (SD) rats (<i>n</i> = 30, 8-week-old) were randomly divided into three groups: LBSO ... Read more >>

Pharm Biol (Pharmaceutical biology)
[2021, 59(1):1314-1325]

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Construction and potential application of bacterial superoxide dismutase expressed in Bacillus subtilis against mycotoxins.

Xueqian Dong, Wei Wang, Tianyi Jiang, Yanmin Zhang, Hongyu Han, Yonggang Zhang, Chunyu Yang,

Oxidative stress, which could be evoked by numerous inducements including mycotoxins like deoxynivalenol (DON), cause severe damages to organisms. Antioxidants are promising protectants against oxidative stress that could be applied in pharmaceutical, cosmetic, and food and feed industries. In this study, a thermostable and acidophilic superoxide dismutase (AaSOD) was used ... Read more >>

PLoS One (PloS one)
[2021, 16(11):e0260047]

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Superoxide dismutase-1 alters the rate of prion protein aggregation and resulting fibril conformation.

Mantas Ziaunys, Kamile Mikalauskaite, Dominykas Veiveris, Andrius Sakalauskas, Vytautas Smirnovas,

The assembly of amyloidogenic proteins into highly-structured fibrillar aggregates is related to the onset and progression of several amyloidoses, including neurodegenerative Alzheimer's or Parkinson's diseases. Despite years of research and a general understanding of the process of such aggregate formation, there are currently still very few drugs and treatment modalities ... Read more >>

Arch Biochem Biophys (Archives of biochemistry and biophysics)
[2021, :109096]

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Effects of Ciliate Infection on the Activities of Two Antioxidant Enzymes (SOD and CAT) in Captive Coral (Goniopora columna) and Evaluation of Drug Therapy.

Chiu-Min Cheng, Yu-Rong Cheng, De-Sing Ding, Ya-Ting Chen, Wei-Ting Sun, Chih-Hung Pan,

Ciliate infection is a serious parasitic disease of coral. Infected coral rots and dies in a short time. In addition to killing corals by infecting them in the oceans, ciliate infection also poses a threat to corals farmed on a large scale. In this study, two antioxidant enzymes (SOD and ... Read more >>

Biology (Basel) (Biology)
[2021, 10(11):]

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Circulating Superoxide Dismutase Concentrations in Obstructive Sleep Apnoea (OSA): A Systematic Review and Meta-Analysis.

Maria Carmina Pau, Arduino Aleksander Mangoni, Elisabetta Zinellu, Gianfranco Pintus, Ciriaco Carru, Alessandro Giuseppe Fois, Pietro Pirina, Angelo Zinellu,

Obstructive Sleep Apnoea (OSA) is characterized by a pro-oxidant state that results from the recurrent hypoxia-reoxygenation cycles. Superoxide dismutase (SOD), a key antioxidant enzyme involved in the detoxification of superoxide radicals, could represent a reliable marker to monitor the antioxidant defences in OSA. In order to capture and critically appraise ... Read more >>

Antioxidants (Basel) (Antioxidants (Basel, Switzerland))
[2021, 10(11):]

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Genome-Wide Superior Alleles, Haplotypes and Candidate Genes Associated With Tolerance on Sodic-Dispersive Soils in Wheat (Triticum Aestivum L.)

Darshan Lal Sharma, Roopali Bhoite, Karyn Reeves, Kerrie Forrest, Rosemary Smith, Mirza A.N.N.U. Dowla,

Sodic-dispersive soils have multiple subsoil constraints including poor soil structure, alkaline pH and subsoil toxic elemental ion concentration, affecting growth and development in wheat. Tolerance is required at all developmental stages to enhance wheat yield potential on such soils. An in-depth investigation of genome-wide associations was conducted using a field ... Read more >>

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Novel pharmacological effects of lecithinized superoxide dismutase on ischemia/reperfusion injury in the kidneys of mice.

Ken-Ichiro Tanaka, Mikako Shimoda, Maho Kubota, Ayaka Takafuji, Masahiro Kawahara, Tohru Mizushima,

Renal ischemia/reperfusion (I/R) injury is a major clinical problem because it can cause acute kidney injury (AKI) or lead to the transition from AKI to chronic kidney disease (CKD). Oxidative stress, which involves the production of reactive oxygen species (ROS), plays an important role in the development and exacerbation of ... Read more >>

Life Sci (Life sciences)
[2021, 288:120164]

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Dietary inclusion of royal jelly modulates gene expression and activity of oxidative stress enzymes in zebrafish.

Ercüment Aksakal, Deniz Ekinci, Claudiu T Supuran,

Here we investigated the effects of different levels of royal jelly in zebrafish (<i>Danio rerio</i>) diets [0.0% (D1); 0.1% (D2); 0.4% (D3); 1.6% (D4) vs 6.4% (D5)] on the activity and expression profiles of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and glutathione S-transferase. Muscle, liver and kidney tissue samples ... Read more >>

J Enzyme Inhib Med Chem (Journal of enzyme inhibition and medicinal chemistry)
[2021, 36(1):885-894]

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Identification of host factors limiting the overexpression of recombinant Cu, Zn superoxide dismutase in Escherichia coli

Shweta Guleria, Robin Joshi, Dharam Singh, Sanjay Kumar,

OBJECTIVE: Superoxide dismutase (SOD) enzyme has implications in modulating the cell’s redox state. The study aims to explore the host genetic factors that limit the heterologous expression of a thermostable SOD from Potentilla atrosanguinea (Pa-SOD) in E. coli. RESULTS: It was observed that the heterologous expression of Pa-SOD in E. ... Read more >>

Biotechnol Lett (Biotechnology letters)
[2021, 42(11):2389-2401]

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MSD2, an apoplastic Mn-SOD, contributes to root skotomorphogenic growth by modulating ROS distribution in Arabidopsis

Huize Chen, Jinsu Lee, Jung-Min Lee, Minsoo Han, Aurelia Emonet, Jiyoun Lee, Xingtian Jia, Yuree Lee,

Reactive oxygen species (ROS) play essential roles as a second messenger in various physiological processes in plants. Due to their oxidative nature, ROS can also be harmful. Thus, the generation and homeostasis of ROS are tightly controlled by multiple enzymes. Membrane-localized NADPH oxidases are well known to generate ROS during ... Read more >>

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Glycinebetaine mitigates drought stress-induced oxidative damage in pears.

Tiequan Niu, Tianpeng Zhang, Yue Qiao, Pengfei Wen, Guangqian Zhai, Enke Liu, Dhafer A Al-Bakre, Mohammad S Al-Harbi, Xiuping Gao, Xinghong Yang,

Glycinebetaine (GB) is an osmoprotectant found in plants under environmental stresses that incorporates drought and is associated with drought tolerance in several plants, such as the woody pear. However, how GB improves drought tolerance in pears remains unclear. In the current study, we explored the mechanism by which GB enhances ... Read more >>

PLoS One (PloS one)
[2021, 16(11):e0251389]

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Antioxidant status in relation to heavy metals induced oxidative stress in patients with polycystic ovarian syndrome (PCOS).

Manal Abudawood, Hajera Tabassum, Atheer H Alanazi, Fatmah Almusallam, Feda Aljaser, Mir Naiman Ali, Naif D Alenzi, Samyah T Alanazi, Manal A Alghamdi, Ghadah H Altoum, Manar A Alzeer, Majed O Alotaibi, Arwa Abudawood, Hazem K Ghneim, Lulu Abdullah Ali Al-Nuaim,

Polycystic ovary syndrome (PCOS) is a global health concern for women of reproductive age, as 6.5% of women worldwide are affected by this syndrome. PCOS is marked by hyperandrogenism, anovulation, menstrual abnormalities, and polycystic ovaries. Metals such as arsenic, cadmium, lead and mercury are considered to be systemic toxicants/human carcinogens ... Read more >>

Sci Rep (Scientific reports)
[2021, 11(1):22935]

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