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Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans.

Jay Gandhi, Giulia Crosio, Anita G Fernandez,

<i>dhc-1(or283ts); mel-28(t1684)</i> double mutants have a severely reduced brood size compared to the wild-type and compared to each single mutant. To determine if this low-fecundity phenotype is associated with oocyte maturity defects, we used markers to assess the maturity of oocytes in the proximal gonad. We studied phosphorylated histone H3, ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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The C. elegans pseudogene sspt-16 (F55A3.7) is required to safeguard germ cells against reprogramming.

Andreas Ofenbauer, Clara Maria Kraus, Baris Tursun,

We recently identified FAcilitates Chromatin Transcription (FACT) as a reprogramming barrier of transcription factor (TF) mediated conversion of germ cells into neurons in <i>C. elegans</i>. FACT is a conserved heterodimer consisting of SPT16 and SSRP1 in mammals. Duplication events during evolution in <i>C. elegans</i> generated two SSRP1 homologs named HMG-3 ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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In vitro screening methods for parasites: the wMicroTracker & the WormAssay.

Emma Gunderson, Christina Bulman, Mona Luo, Judy Sakanari,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

Cited: 2 times

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A CRISPR/Cas9-generated cdc-7 loss of function mutation does not cause temperature-dependent fertility defects.

Heather Currey, Nicole Liachko,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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Delivery of Double-Stranded RNAs (dsRNAs) Produced by Escherichia coli HT115(DE3) for Nontransgenic RNAi-Based Insect Pest Management.

Mabel L Taracena, Isabella Garcia Caffaro, Gabriela O Paiva-Silva, Pedro L Oliveira, Pedro A Rendon, Ellen M Dotson, Pamela M Pennington,

RNA interference (RNAi) is a powerful mechanism that can be exploited not only for physiology research but also for designing insect pest management approaches. Some insects cause harm by vectoring diseases dangerous to humans, livestock, or plants or by damaging crops. For at least a decade now, different insect control ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2022, 2360:279-294]

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Bioassays for the Evaluation of Target Neutralization and Complement-Dependent Cytotoxicity (CDC) of Therapeutic Antibodies.

Nohemí Salinas-Jazmín, Emilio Medina-Rivero, Marco Antonio Velasco-Velázquez,

Therapeutic monoclonal antibodies (mAbs) are complex bioengineered proteins that require to be routinely characterized with robust and reliable bioassays. Infliximab was the first anti-TNFα mAb approved for use in humans and its use has revolutionized the treatment TNF-mediated inflammatory disorders. The mechanism of action (MOA) of infliximab involves its binding ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2022, 2313:281-294]

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A Chinese patient with the clinical features of Parkinson's disease contains a single copy of octarepeat deletion in PRNP case report.

Qi Shi, Xiao-Jing Shen, Li-Ping Gao, Kang Xiao, Wei Zhou, Yuan Wang, Cao Chen, Xiao-Ping Dong,

Insertion or deletion of single copy of octapeptide repeat (OR) in human PrP protein are considered as polymorphism, while of insertions of more numbers of OR and deletion of two copies of OR are associated with genetic prion diseases.Here, we reported a 58-year-old female patient who displayed clinical manifestations of ... Read more >>

Prion (Prion)
[2021, 15(1):121-125]

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Creating a 'choose your topic' massive open online course: an innovative and flexible approach to delivering injury prevention education.

Nadine I Ibrahim, Lauren Bohm, Jessica S Roche, Sarah A Stoddard, Rebecca M Quintana, Jennifer Vetter, Jeffrey Bennett, Beth Costello, Patrick M Carter, Rebecca Cunningham, Andrew N Hashikawa,

<h4>Background</h4>A pediatric injury prevention course has not been available as a massive open online course (MOOC). Creating a comprehensive topic course is particularly challenging because the traditional, week-by-week linear curriculum design is often a barrier to learners interested in only specific topics. We created a novel, flexible course as both ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1955646]

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The prevalence of CRF55_01B among HIV-1 strain and its connection with traffic development in China.

Mengze Gan, Shan Zheng, Jingjing Hao, Yuhua Ruan, Lingjie Liao, Yiming Shao, Yi Feng, Hui Xing,

CRF55_01B is a relatively "young" HIV strain. At present, we do not know much about its transmission characteristics in China. So, to describe the transmission characteristics of CRF55_01B strain among provinces and HIV infected people, and to analyze the reasons for its rapid epidemic in China, a total of 1237 ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):256-265]

Cited: 1 time

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Sociocultural factors contributing to waterpipe tobacco smoking among adolescents and young adult women: a qualitative study in Iran.

Zeinab Makvandi, Firoozeh Mostafavi, Saeed Bashirian, Fereshteh Zamani-Alavijeh, Roya Kelishadi,

<b>Purpose</b>: Waterpipe tobacco smoking (WTS) is currently a serious and growing public health threat in the world, especially in adolescents and young women. The aim of the study was to explore sociocultural factors contributing to WTS among adolescents and young adult women in Iran. <b>Methods</b>: This qualitative study was conducted ... Read more >>

Int J Qual Stud Health Well-being (International journal of qualitative studies on health and well-being)
[2021, 16(1):1857043]

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Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system.

Hsing-Yi Chung, Ming-Jr Jian, Chih-Kai Chang, Jung-Chung Lin, Kuo-Ming Yeh, Chien-Wen Chen, Sheng-Kang Chiu, Yi-Hui Wang, Shu-Jung Liao, Shih-Yi Li, Shan-Shan Hsieh, Shih-Hung Tsai, Cherng-Lih Perng, Ji-Rong Yang, Ming-Tsan Liu, Feng-Yee Chang, Hung-Sheng Shang,

SARS-CoV-2 has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of upper respiratory tract infection. We developed and validated a rapid total nucleic acid extraction method ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):161-166]

Cited: 6 times

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A call for change from impersonal risk assessment to a relational approach: professionals' reflections on the national guidelines for suicide prevention in mental health care in Norway.

Kristin Espeland, Heidi Hjelmeland, Birthe Loa Knizek,

<h4>Purpose</h4>The purpose of the study is to explore how professionals working with suicide prevention experience the influence of the national guidelines on mental healthcare, and to gather recommendations for which steps to take next.<h4>Methods</h4>This is a qualitative study with an explorative design. We interviewed 22 professionals responsible for implementing suicide ... Read more >>

Int J Qual Stud Health Well-being (International journal of qualitative studies on health and well-being)
[2021, 16(1):1868737]

Cited: 1 time

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Adherence to oral anticoagulation in ischemic stroke patients with atrial fibrillation.

Paula Tiili, Ioannis Leventis, Janne Kinnunen, Ida Svedjebäck, Mika Lehto, Efstathia Karagkiozi, Dimitrios Sagris, George Ntaios, Jukka Putaala,

<h4>Background</h4>Non-vitamin K antagonist oral anticoagulants (NOAC) have superior safety and comparable efficacy profile compared to vitamin-K antagonists (VKAs), with more convenient dosing schemes. However, issues with adherence to the NOACs remain unsolved.<h4>Aims</h4>We sought to investigate the adherence to oral anticoagulation (OAC) and baseline factors associated with poor adherence after ischaemic ... Read more >>

Ann Med (Annals of medicine)
[2021, 53(1):1613-1620]

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Transfusion-dependent beta thalassemia in Afghanistan: current evidence amid COVID-19 and future recommendations.

Shohra Qaderi, Sayed Hamid Mousavi, Attaullah Ahmadi, Shamim Arif, Shekiba Madadi, Sohrab Ayoubi, Don Eliseo Lucero-Prisno,

Hematology (Hematology (Amsterdam, Netherlands))
[2021, 26(1):432-434]

Cited: 1 time

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Making sense of fidelity: young Africans' cross-national and longitudinal representations of fidelity and infidelity in their HIV-related creative narratives, 1997-2014.

Robyn Singleton, Manon Billaud, Haley McLeod, Georges Tiendrebeogo, Fatim Dia, Chris Obong'o, Siphiwe Nkambule-Vilakati, Benjamin Mbakwem, Gaelle Sabben, Kate Winskell,

Mutual fidelity and partner reduction have been identified as key behavioural strategies to prevent HIV transmission in sub-Saharan Africa, particularly following recognition of the role that multiple concurrent sexual partnerships play in driving generalised HIV epidemics. We analysed social representations of fidelity and infidelity in a sample of 1,343 narratives ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):86-97]

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Influence of early-life body mass index and systolic blood pressure on left ventricle in adulthood - the Cardiovascular Risk in Young Finns Study.

Jarkko S Heiskanen, Jussi A Hernesniemi, Saku Ruohonen, Nina Hutri-Kähönen, Mika Kähönen, Eero Jokinen, Päivi Tossavainen, Merja Kallio, Tomi Laitinen, Terho Lehtimäki, Jorma Viikari, Markus Juonala, Jaakko Nevalainen, Olli T Raitakari,

<h4>Background</h4>Increased left ventricular mass (LVM) predicts cardiovascular events and mortality. The objective of this study was to determine whether early-life exposures to body mass index (BMI) and systolic blood pressure (SPB) affects the left ventricular structure in adulthood.<h4>Methods</h4>We used longitudinal data from a 31-year follow-up to examine the associations between ... Read more >>

Ann Med (Annals of medicine)
[2021, 53(1):160-168]

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Antibodies to SARS-CoV-2 in dogs and cats, USA.

Subarna Barua, Monirul Hoque, Folasade Adekanmbi, Patrick Kelly, Melinda Jenkins-Moore, Mia Kim Torchetti, Kelly Chenoweth, Theresa Wood, Chengming Wang,

To provide more complete data on SARS-CoV-2 infections in dogs and cats in the U.S., we conducted a serosurvey on convenience serum samples from dogs (<i>n</i>=1336) and cats (<i>n</i>=956) collected from 48 states of the USA in 2020. An ELISA targeting the antibody against nucleocapsid identified eleven positive and two ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):1669-1674]

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Knowledge, attitudes and prevention practices regarding HIV/AIDS among barbers in Ho municipality, Ghana.

Mercy Demaris Quarm, Jacqueline Mthembu, Khangelani Zuma, Elvis Enowbeyang Tarkang,

The barbing industry poses particular public health risks if it is not conducted in a safe and hygienic manner. These risks can lead to the transmission of infectious diseases like the Human Immunodeficiency Virus (HIV) to the barbers or their clients. This study investigated the knowledge, attitudes and prevention practices ... Read more >>

SAHARA J (SAHARA J : journal of Social Aspects of HIV/AIDS Research Alliance)
[2021, 18(1):42-51]

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Human challenge trials to assess the efficacy of currently approved COVID-19 vaccines against SARS-CoV-2 variants.

Shan Su, Yiming Shao, Shibo Jiang,

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):439-441]

Cited: 2 times

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Using molecular transmission networks to understand the epidemic characteristics of HIV-1 CRF08_BC across China.

Kang Li, Meiliang Liu, Huanhuan Chen, Jianjun Li, Yanling Liang, Yi Feng, Hui Xing, Yiming Shao,

HIV-1 CRF08_BC has become a major epidemic in heterosexuals and intravenous drug users (IDUs) in southern China. In order to evaluate the trends of its epidemic and facilitate targeted HIV prevention, we constructed the genetic transmission networks based on its <i>pol</i> sequences, derived from the National HIV Molecular Epidemiology Survey. ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):497-506]

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Attitudes, behaviours and strategies towards obesity patients in primary care: A qualitative interview study with general practitioners in Germany.

Julian Wangler, Michael Jansky,

<h4>Background</h4>Obesity poses severe challenges for the health care system. GPs are in an advantageous position to contribute to preventing obesity by diagnosing patients and initiating treatment. Sporadic studies have shown that attitudes towards obesity management in primary care can have a major influence on treating patients successfully.<h4>Objectives</h4>The study focuses on ... Read more >>

Eur J Gen Pract (The European journal of general practice)
[2021, 27(1):27-34]

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Early Laboratory Preparedness of the Korea Disease Control and Prevention Agency and Response to Unknown Pneumonia Outbreak from Wuhan, China, in January 2020.

Il-Hwan Kim, Byung-Hak Kang, Seung Hee Seo, Ye Eun Park, Gab Jung Kim, Sang Won Lee, Jun Hyeong Jang, Su Kyoung Jo, Jun Ho Jeon, Jeong-Min Kim, Yoon-Seok Chung, Myung-Guk Han, Sang-Oun Jung, Junyoung Kim, Kyu-Jam Hwang, Cheon-Kwon Yoo, Gi-Eun Rhie,

We report the response process of the Laboratory Analysis Task Force (LATF) for Unknown Disease Outbreaks (UDOs) at the Korea Disease Control and Prevention Agency (KDCA) during January 2020 to coronavirus disease 2019 (COVID-19), which developed as a UDO in Korea. The advanced preparedness offered by the laboratory diagnostic algorithm ... Read more >>

Ann Lab Med (Annals of laboratory medicine)
[2021, 41(6):532-539]

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A host-based whole genome sequencing study reveals novel risk loci associated with severity of influenza A(H1N1)pdm09 infection.

Mo Li, Yongkun Chen, Tao Chen, Shixiong Hu, Luan Chen, Lu Shen, Fangcai Li, Jing Yang, Yan Sun, Dayan Wang, Lin He, Shengying Qin, Yuelong Shu,

Influenza A(H1N1)pdm09 virus has remained in a seasonal circulation since being recognized in 2009. Although it followed a mild course in most patients, in others it caused a series of severe clinical illnesses. Epidemiologic studies have implicated that host factors have a major influence on the disease severity of influenza ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):123-131]

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Intensified antibody response elicited by boost suggests immune memory in individuals administered two doses of SARS-CoV-2 inactivated vaccine.

Yun Liao, Ying Zhang, Heng Zhao, Jing Pu, Zhimei Zhao, Dandan Li, Shengtao Fan, Li Yu, Xingli Xu, Lichun Wang, Guorun Jiang, Longding Liu, Qihan Li,

Neutralizing antibodies in the subjects of an inactivated SARS-CoV-2 vaccine clinical trial showed a decreasing trend over months. An investigation studying the third immunization suggested that the waning of neutralizing antibodies in individuals administered two doses of inactivated vaccine does not mean the disappearance of immunity. ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):1112-1115]

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The COVID-19-designated hospitals in China: preparing for public health emergencies.

Qian Li, Lin Wang, Bangfang Wang, Hongzhou Lu,

The designated hospitals are another health approach besides Fangcang shelter hospitals and newly built temporary hospitals for responding to COVID-19 epidemic in China. Faced with the emergency situation, about 1512 hospitals from 363 cities have been designated in China to tackle the spread of COVID-19. They were local hospitals repurposed ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, 10(1):998-1001]

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