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Highly improved cloning efficiency for plasmid-based CRISPR knock-in in C. elegans.

Ella DeMott, Daniel J Dickinson, Ryan Doonan,

Plasmid-based CRISPR knock-in is a streamlined, scalable, and versatile approach for generating fluorescent protein tags in <i>C. elegans</i> (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). However, compared to more recent protocols that utilize commercially available Cas9/RNP products and linear DNA repair templates (Dokshin <i>et al</i>. 2018; Ghanta and Mello ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Overactive EGF signaling suppresses a C. elegans pnc-1 egg-laying phenotype independent of known signaling mediators.

Matt Crook, Wendy Hanna-Rose,

Nicotinamide recycling is critical to the development and function of <i>Caenorhabditis elegans</i>. Excess nicotinamide in a <i>pnc-1</i> nicotinamidase mutant causes the necrosis of uv1 and OLQ cells and a highly penetrant egg laying defect. An EGF receptor (<i>let-23</i>) gain-of-function mutation suppresses the Egl phenotype in <i>pnc-1</i> animals. However, gain-of-function mutations ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans.

George Huang, Bailey de Jesus, Alex Koh, Sara Blanco, Aubrie Rettmann, Ella DeMott, Melynda Sylvester, Cassie Ren, Carrie Meng, Skye Waterland, Anita Rhodes, Persephone Alicea, Abbey Flynn, Daniel J Dickinson, Ryan Doonan,

Streamlined, selection-based CRISPR knock-in protocols for <i>C. elegans</i> were first introduced six years ago (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). Though these selection-based approaches are powerful, one drawback has been the requirement to inject large numbers of P0 worms (~30-60 per gene target). We have found that a ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans.

Jay Gandhi, Giulia Crosio, Anita G Fernandez,

<i>dhc-1(or283ts); mel-28(t1684)</i> double mutants have a severely reduced brood size compared to the wild-type and compared to each single mutant. To determine if this low-fecundity phenotype is associated with oocyte maturity defects, we used markers to assess the maturity of oocytes in the proximal gonad. We studied phosphorylated histone H3, ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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The C. elegans pseudogene sspt-16 (F55A3.7) is required to safeguard germ cells against reprogramming.

Andreas Ofenbauer, Clara Maria Kraus, Baris Tursun,

We recently identified FAcilitates Chromatin Transcription (FACT) as a reprogramming barrier of transcription factor (TF) mediated conversion of germ cells into neurons in <i>C. elegans</i>. FACT is a conserved heterodimer consisting of SPT16 and SSRP1 in mammals. Duplication events during evolution in <i>C. elegans</i> generated two SSRP1 homologs named HMG-3 ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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In vitro screening methods for parasites: the wMicroTracker & the WormAssay.

Emma Gunderson, Christina Bulman, Mona Luo, Judy Sakanari,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

Cited: 2 times

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A CRISPR/Cas9-generated cdc-7 loss of function mutation does not cause temperature-dependent fertility defects.

Heather Currey, Nicole Liachko,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response.

Quanquan Shen, Qian Chen, Yang Liu, Xiang Xue, Xiaogang Shen, Qiang He, Guokun Wang, Fei Han,

<h4>Context</h4>Vascular calcification is a major complication of chronic renal failure, which has been identified as an active process partly driven by osteogenic transition of vascular smooth muscle cells (VSMCs). Aspirin could prevent cardiomyocyte damage by inducing heat shock response.<h4>Objective</h4>This study investigates the effect of aspirin on alleviating VSMC calcification.<h4>Materials and ... Read more >>

Pharm Biol (Pharmaceutical biology)
[2022, 60(1):17-24]

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Forge AHEAD with stricter criteria in future trials of embolic stroke of undetermined source.

David Z Rose, Scott E Kasner,

Neural Regen Res (Neural regeneration research)
[2022, 17(5):1009-1010]

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Embolic stroke of undetermined source: identification of patient subgroups for oral anticoagulation treatment.

Isabell Greeve, Wolf-Rüdiger Schäbitz,

Neural Regen Res (Neural regeneration research)
[2022, 17(5):1005-1006]

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Repurposing verapamil for prevention of cognitive decline in sporadic Alzheimer's disease.

Heba A Ahmed, Tauheed Ishrat,

Neural Regen Res (Neural regeneration research)
[2022, 17(5):1018-1019]

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Serotype Distribution and Antimicrobial Resistance of Salmonella Isolates in Korea between 2016 and 2017.

Si Hyun Kim, Gyung-Hye Sung, Eun Hee Park, In Yeong Hwang, Gyu Ri Kim, Sae Am Song, Hae Kyung Lee, Young Uh, Young Ah Kim, Seok Hoon Jeong, Jong Hee Shin, Kyeong Seob Shin, Jaehyeon Lee, Joseph Jeong, Young Ree Kim, Dongeun Yong, Miae Lee, Yu Kyung Kim, Nam Hee Ryoo, Seungok Lee, Jayoung Kim, Sunjoo Kim, Hyun Soo Kim, Jeong Hwan Shin,

<i>Salmonella</i> is one of the major causes of food-borne infections. We investigated the serotype distribution and antimicrobial resistance of <i>Salmonella</i> isolates collected in Korea between January 2016 and December 2017. In total, 669 <i>Salmonella</i> isolates were collected from clinical specimens at 19 university hospitals. Serotyping was performed according to the ... Read more >>

Ann Lab Med (Annals of laboratory medicine)
[2022, 42(2):268-273]

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Prevalence of Concussion-Related Policies and Practices Among Public School Districts in the United States, 2012 and 2016.

Gabrielle F Miller, Lara DePadilla, Sherry Everett Jones, Michael Lionbarger, Sally Thigpen,

<h4>Background</h4>Beginning in 2009, there was an increase in the number of states with laws addressing 3 different components of youth sports-related concussion prevention and management: concussion education, removal from play, and medical clearance requirements before allowing an athlete to return to play. Schools are an important setting to implement policies ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):E194-E197]

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Secondary and Tertiary Prevention: Medical Rehabilitation.

Karin B Fieten, Swen Malte John, Dennis Nowak,

Allergies are a major public health burden, and targeted measures are required in terms of prevention and treatment. The most common allergic conditions encompass atopic dermatitis (AD), food allergy (FA), allergic asthma (AA), and allergic rhino-conjunctivitis (AR). Primary prevention aims at preventing the onset of allergic disease, before the disease ... Read more >>

Handb Exp Pharmacol (Handbook of experimental pharmacology)
[2022, 268:449-470]

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Bioassays for the Evaluation of Target Neutralization and Complement-Dependent Cytotoxicity (CDC) of Therapeutic Antibodies.

Nohemí Salinas-Jazmín, Emilio Medina-Rivero, Marco Antonio Velasco-Velázquez,

Therapeutic monoclonal antibodies (mAbs) are complex bioengineered proteins that require to be routinely characterized with robust and reliable bioassays. Infliximab was the first anti-TNFα mAb approved for use in humans and its use has revolutionized the treatment TNF-mediated inflammatory disorders. The mechanism of action (MOA) of infliximab involves its binding ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2022, 2313:281-294]

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The Impact of Covid-19 State Closure Orders on Consumer Spending, Employment, and Business Revenue.

Christopher Dunphy, Gabrielle F Miller, Ketra Rice, Linda Vo, Gregory Sunshine, Russell McCord, Mara Howard-Williams, Fátima Coronado,

<h4>Context</h4>In response to the COVID-19 pandemic, states across the United States implemented various strategies to mitigate transmission of SARS-CoV-2 (the virus that causes COVID-19).<h4>Objective</h4>To examine the effect of COVID-19-related state closures on consumer spending, business revenue, and employment, while controlling for changes in COVID-19 incidence and death.<h4>Design</h4>The analysis estimated a ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):43-49]

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Progress in Cancer Control in Maryland: 1985-2015.

Victoria Brun, Elizabeth A Platz, Thuy Nguyen, Sara Valek, Donna Gugel, Karen Warmkessel, Norma F Kanarek,

<h4>Purpose</h4>Maryland historically had a high cancer burden, which prompted the implementation of aggressive cancer control strategies. We examined the status of cancer in Maryland and work under the current and previous editions of the MD Comprehensive Cancer Control Plan.<h4>Methods</h4>We examined the prevalence of cancer mortality, cancer incidence, and cancer-related behaviors ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):E23-E32]

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Effective Ways to Prevent Allergic Diseases: Where Do We Stand?

Katja Landgraf-Rauf, Erika von Mutius,

Since allergic diseases are of great public health relevance, effective primary prevention strategies are urgently needed. This chapter gives an overview of existing primary prevention programs on environmental exposures and dietary strategies based on epidemiological studies which have defined risk- and protective factors for the development of allergic diseases.The allergy ... Read more >>

Handb Exp Pharmacol (Handbook of experimental pharmacology)
[2022, 268:437-448]

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The Health and Economic Benefits of Tests That Predict Future Progression to Tuberculosis Disease.

Nicolas A Menzies, Sourya Shrestha, Andrea Parriott, Suzanne M Marks, Andrew N Hill, David W Dowdy, Priya B Shete, Ted Cohen, Joshua A Salomon,

<h4>Background</h4>Effective targeting of latent tuberculosis infection (LTBI) treatment requires identifying those most likely to progress to tuberculosis (TB). We estimated the potential health and economic benefits of diagnostics with improved discrimination for LTBI that will progress to TB.<h4>Methods</h4>A base case scenario represented current LTBI testing and treatment services in the ... Read more >>

Epidemiology (Epidemiology (Cambridge, Mass.))
[2022, 33(1):75-83]

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Modeling the Transmission of COVID-19: Impact of Mitigation Strategies in Prekindergarten-Grade 12 Public Schools, United States, 2021.

Gabrielle F Miller, Bradford Greening, Ketra L Rice, Aziza Arifkhanova, Martin I Meltzer, Fátima Coronado,

<h4>Background</h4>Schools are an integral part of the community; however, congregate settings facilitate transmission of SARS-CoV-2, presenting a challenge to school administrators to provide a safe, in-school environment for students and staff.<h4>Methods</h4>We adapted the Centers for Disease Control and Prevention's COVIDTracer Advanced tool to model the transmission of SARS-CoV-2 in a ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):25-35]

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Comparison of Census Tract-Level Chronic Disease Prevalence Estimates From 500 Cities and Local Health Claims Data.

Alyssa Monaghan, Lynda Jones, LuAnn Brink, Karen Hacker,

<h4>Objectives</h4>To compare city and census tract-level diabetes and hypertension prevalence using 500 Cities Project modeled estimates from the Centers for Disease Control and Prevention (CDC) and insurance claims data.<h4>Methods</h4>Insurance claims by census tract were collected from 3 local health plans for the city of Pittsburgh, Pennsylvania, for 2015-2016; conditions were ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):E92-E95]

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The Power of Connections: AAP COVID-19 ECHO Accelerates Responses During a Public Health Emergency.

Henry H Bernstein, Trisha Calabrese, Peter Corcoran, Laurence E Flint, Flor M Munoz, , ,

<h4>Context</h4>Pediatric providers across the United States have sought guidance on how to care for the nation's children during the uncertain historic times of the COVID-19 pandemic. The health care community has been challenged by the unprecedented burden of caring for patients when they have evolving guidelines and limited information about ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):E1-E8]

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Receipt and Timeliness of Newborn Hearing Screening and Diagnostic Services Among Babies Born in 2017 in 9 States.

Xidong Deng, Suhana Ema, Craig Mason, Ashley Nash, Eric Carbone, Marcus Gaffney,

<h4>Context</h4>By providing timely services at all steps along the continuum of the early hearing detection and intervention (EHDI) process, providers may be able to lessen potential adverse effects of late identification of hearing loss on children's language development.<h4>Objective</h4>To examine the timeliness of key events in the EHDI process from birth ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):E100-E108]

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Occupational Respiratory Allergy: Risk Factors, Diagnosis, and Management.

Monika Raulf,

Occupational allergies are among the most common recorded occupational diseases. The skin and the upper and lower respiratory tract are the classical manifestation organs. More than 400 occupational agents are currently documented as being potential "respiratory sensitizers" and new reported causative agents are reported each year. These agents may induce ... Read more >>

Handb Exp Pharmacol (Handbook of experimental pharmacology)
[2022, 268:213-225]

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Willingness to Pay for Select Tick-Borne Disease Prevention Measures in Endemic Areas.

Sara Niesobecki, Heather Rutz, Linda Niccolai, Sarah Hook, Katherine Feldman, Alison Hinckley,

Tick-borne diseases (TBDs) are increasing despite prevention recommendations. We explored whether cost is a barrier to prevention use in Connecticut and Maryland, using a cross-sectional survey. Respondents were queried regarding their willingness to pay for chemical, natural, and rodent-targeted yard pesticide treatments and permethrin-treated clothing. We evaluated associations between demographics, ... Read more >>

J Public Health Manag Pract (Journal of public health management and practice : JPHMP)
[2022, 28(1):E37-E42]

Cited: 1 time

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