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CRISPRcruncher: A tool for engineering restriction sites into coding regions.

Samuel F Fay, David S Fay, Vikram E Chhatre,

CRISPR/Cas9 genome editing strategies often rely on the placement of an introduced restriction endonuclease (RE) site adjacent to the genomic edit of interest. This allows for rapid initial PCR-based detection of cells and organisms containing the edit of interest and may also be used for subsequent genotyping. Nevertheless, engineering RE ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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2018 Update on Protein-Protein Interaction Data in WormBase.

Jaehyoung Cho, Christian Grove, Kimberly Van Auken, Juancarlos Chan, Sibyl Gao, Paul Sternberg,

MicroPubl Biol (microPublication biology)
[2018, 2018:]

Cited: 1 time

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"If the reindeer die, everything dies": The mental health of a Sámi community exposed to a mining project in Swedish Sápmi.

Hanna Blåhed, Miguel San Sebastián,

In 2006, a British mining company started the process of extracting ore from Gállok/Kallak, in Swedish Sápmi. These grounds are used all year round for reindeer herding by the Sámi community Jåhkågasska tjiellde. While environmental impact assessments should be conducted by law in any development project in Sweden, the health ... Read more >>

Int J Circumpolar Health (International journal of circumpolar health)
[2021, 80(1):1935132]

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Predicting candidate therapeutic drugs for sepsis-induced acute respiratory distress syndrome based on transcriptome profiling.

Jiawei Ma, Qianqian Li, Dandan Ji, Liang Hong, Lei Luo,

Sepsis-induced acute respiratory distress syndrome (ARDS) remains a major threat to human health without effective therapeutic drugs. Previous studies demonstrated the power of gene expression profiling to reveal pathological changes associated with sepsis-induced ARDS. However, there is still a lack of systematic data mining framework for identifying potential targets for ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):1369-1380]

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Data mining-based study of collagen type III alpha 1 (COL3A1) prognostic value and immune exploration in pan-cancer.

Hanyu Zhang, Cheng Ding, Yatong Li, Cheng Xing, Shunda Wang, Zhangping Yu, Lixin Chen, Pengyu Li, Menghua Dai,

The extracellular matrix (ECM) shows an essential effect during the occurrence and procession of human cancers. Type III collagen is a crucial component of ECM. Collagen Type III Alpha 1(COL3A1) is aberrantly expressed in a variety of cancers. Nevertheless, the role of COL3A1 in pan-cancer stays unidentified. In this study, ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):3634-3646]

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Elevated Ras related GTP binding B (RRAGB) expression predicts poor overall survival and constructs a prognostic nomogram for colon adenocarcinoma.

Jianjia Xiao, Qingqing Liu, Weijie Wu, Ying Yuan, Jie Zhou, Jieyu Shi, Shaorong Zhou,

Currently, no articles have explored the roles of RRAGB gene in the occurrence and development of cancer. By means of The Cancer Genome Atlas (TCGA) data mining, we found that this gene might be a novel prognostic predictor for colon adenocarcinoma (COAD). Hence, this article was carried out to explore ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):4620-4632]

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An epithelial-mesenchymal transition-related long non-coding RNA signature to predict overall survival and immune microenvironment in kidney renal clear cell carcinoma.

You-Peng Zhang, Yong-Biao Cheng, Sen Li, Ning Zhao, Zhao-Hui Zhu,

Kidney renal clear cell carcinoma (ccRCC) is a malignant tumor originating from renal tubular epithelium, lncRNAs can regulate the occurrence and development of EMT by targeting EMT transcription factors. We constructed a new survival signature based on EMT-related differentially expressed lncRNAs obtained from the Cancer Genome Atlas (TCGA-KIRC). We first ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):555-564]

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Multi-target mechanism of Tripteryguim wilfordii Hook for treatment of ankylosing spondylitis based on network pharmacology and molecular docking.

Jing Zhang, Yiting Zhou, Zhiyuan Ma,

<h4>Background</h4><i>Tripteryguim wilfordii</i> Hook (TWH) has significant anti-inflammatory and immunosuppressive properties and is widely used for treating autoimmune and inflammatory diseases. However, the multi-target mechanism of TWH on ankylosing spondylitis (AS) remains to be elucidated.<h4>Methods</h4>Active components and their target proteins were screened from the Traditional Chinese Medicine Systems Pharmacology Database and ... Read more >>

Ann Med (Annals of medicine)
[2021, 53(1):1090-1098]

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Transforming the wellbeing focus in education: A document analysis of policy in Aotearoa New Zealand.

Joanna Higgins, Suskya Goodall,

<b>Purpose</b>: Understanding the nature of wellbeing as multidimensional and complex provides a policy window to generate a strengths-based policy orientation to promote wellbeing in education settings. The purpose of this exploratory paper is to map how wellbeing is interpreted across public education policy documents in Aotearoa New Zealand. <b>Method</b>: To ... Read more >>

Int J Qual Stud Health Well-being (International journal of qualitative studies on health and well-being)
[2021, 16(1):1879370]

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Relationship between hepatocellular carcinoma and depression via online database analysis.

Tiantian Han, Yingchun Zhou, Danhua Li,

There may be a mutually reinforcing relationship between hepatocellular carcinoma (HCC) and depression, but the mechanism is unknown. This study used bioinformatics to evaluate the relationship between HCC and depression at the genetic level. Genes associated with HCC and depression were obtained from pubmed2ensemble. Overlapping genes were annotated by gene ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):1689-1697]

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Microorganisms maintain C:N stoichiometric balance by regulating the priming effect in long-term fertilized soils

Zhenke Zhu, Juan Zhou, Muhammad Shahbaz, Haiming Tang, Shoulong Liu, Wenju Zhang, Hongzhao Yuan, Ping Zhou, Hattan Alharbi, Jinshui Wu, Yakov Kuzyakov, Tida Ge,

Labile carbon (C) inputs affect the soil carbon:nitrogen (C:N) ratio and microbial stoichiometric homeostasis, which control the intensity and direction of the priming effect (PE). Here, we clarified how soil microorganisms regulate enzyme production and PE to maintain the C:N stoichiometric balance. Specifically, we conducted an incubation experiment by adding ... Read more >>

Appl Soil Ecol (Applied soil ecology.)
[2021, 167:Not Available]

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Food bioactive small molecule databases: Deep boosting for the study of food molecular behaviors

Zheng-Fei Yang, Ran Xiao, Fei-Jun Luo, Qin-Lu Lin, Defang Ouyang, Jie Dong, Wen-Bin Zeng,

Food Bioactive Small Molecule Databases (FBSMDs) provide valuable data resources for the study of molecular behavior, molecular nutrition, as well as the R&D of drugs and health products. In this review, 89 food databases were collected and 32 of them were classified as FBSMDs. Furthermore, we summarized their research contents ... Read more >>

(Innovative food science & emerging technologies.)
[2021, 66:Not Available]

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Paepalanthus fabianeae (Eriocaulaceae): A New Microendemic Species from a Morphologically Coherent Clade

Caroline Oliveira Andrino, Marcelo Fragomeni Simon, Jair Eustáquio Quintino Faria, André Luiz da Costa Moreira, Paulo Takeo Sano,

We describe and illustrate Paepalanthus fabianeae, a new species of Eriocaulaceae from the central portion of the Espinhaço Range in Minas Gerais, Brazil. Previous phylogenetic evidence based on analyses of nuclear (ITS and ETS) and plastid (trnL-trnF and psba-trnH) sequences revealed P. fabianeae as belonging to a strongly supported and ... Read more >>

Syst Bot (Systematic botany.)
[2021, 45(4):794-801]

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Immune cell infiltration landscape and immune marker molecular typing in preeclampsia.

YiLin Meng, Chuang Li, Cai-Xia Liu,

Preeclampsia (PE) is an important topic in obstetrics. In this study, we used weighted gene co-expression network analysis (WGCNA) to screen the key modules related to immune cell infiltration and to identify the hub genes for the molecular subtyping of PE. We first downloaded a set of PE transcriptional data ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):540-554]

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Leisure and health benefits associated with acculturation among Western international students living in South Korea.

Junhyoung Kim, Se-Hyuk Park, May Kim, Hsueh-Wen Chow, Sua Han,

<b>Purpose</b>: Prior research provides limited information on the roles that leisure can play among Western international students studying in Eastern countries. Exploring this directional difference can provide its implications for Western international student support. Therefore, the purpose of this study is to explore leisure behaviours and leisure benefits associated with ... Read more >>

Int J Qual Stud Health Well-being (International journal of qualitative studies on health and well-being)
[2021, 16(1):1945725]

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Impact of Bupivacaine on malignant proliferation, apoptosis and autophagy of human colorectal cancer SW480 cells through regulating NF-κB signaling path.

Bingwu Liu, Xinfeng Yan, Zuojia Hou, Lei Zhang, Duwen Zhang,

To probe into the impact of Bupivacaine on colorectal cancer (CRC) proliferation, apoptosis, and autophagy through regulating the NF-κB signaling pathway. Our work treated CRC cells with Bupivacaine, detected cell vitality through MTT assay, apoptosis through flow cytometry, cell migration through wound healing assay, NF-κB activity through immunofluorescence, inflammatory factor ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):2723-2733]

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Evaluating the Effective Factors of Hospital Rooms on Patients' Recovery Using the Data Mining Method.

S Nikabadi, H Zabihi, A Shahcheraghi,

<h4>Objectives</h4>This study aims to investigate the effective environmental factors of hospital rooms in patients' recovery through data mining techniques.<h4>Background</h4>Previous studies have shown the positive effect of the interior environment of the hospitals on patients' recovery. The methods of these studies were mainly based on the evidence and patients' perception while ... Read more >>

HERD (HERD)
[2021, :19375867211031305]

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Early Prediction of Student Learning Performance Through Data Mining: A Systematic Review.

Javier López-Zambrano, Juan A Lara Torralbo, Cristobal Romero,

<h4>Background</h4>Early prediction of students&rsquo; learning performance using data mining techniques is an important topic these days. The purpose of this literature review is to provide an overview of the current state of research in that area.<h4>Method</h4>We conducted a literature review following a two-step procedure, looking for papers using the major ... Read more >>

Psicothema (Psicothema)
[2021, 33(3):456-465]

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Evaluation of redevelopment priority of abandoned industrial and mining land based on heavy metal pollution.

Xing Gao, Junping Tian, Zheng Huo, Yanbin Wu, Chenxi Li,

Heavy metal contamination in soil is an important factor affecting the determination of safe redevelopment methods for industrial and mining land. In this paper, the soil environment of a typical mining city in northern China was taken as the research object, 148 surface soil samples were collected and the contents ... Read more >>

PLoS One (PloS one)
[2021, 16(7):e0255509]

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Quantification of Potentially Toxic Elements in Degraded Mining Soils and Medicinal Plants: A Case Study of Indus Kohistan Region Northern Pakistan

Waseem sardar, Aziz Ur Rahman, javed nawab, Sardar Khan, Abid Ali, Zia Ur Rahman, Shams Ali Baig, Muhammad Qayash Khan,

In recent years, a series of environmental and ecological problems have occurred due to enhanced anthropogenic disturbances for precious minerals mining. Traditional medicines have become an important pillar in national homeopathic treatment system especially in mountainous environment of developing countries. The current study investigates the level of potentially toxic elements ... Read more >>

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Using Text Mining Techniques to Identify Health Care Providers With Patient Safety Problems: Exploratory Study.

Iris Hendrickx, Tim Voets, Pieter van Dyk, Rudolf B Kool,

<h4>Background</h4>Regulatory bodies such as health care inspectorates can identify potential patient safety problems in health care providers by analyzing patient complaints. However, it is challenging to analyze the large number of complaints. Text mining techniques may help identify signals of problems with patient safety at health care providers.<h4>Objective</h4>The aim of ... Read more >>

J Med Internet Res (Journal of medical Internet research)
[2021, 23(7):e19064]

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Mapping annual land disturbance and reclamation in rare-earth mining disturbance region using temporal trajectory segmentation.

Zhenbang Wu, Hengkai Li, Yuqing Wang,

Rare-earth mining has caused extensive damage to soil, vegetation, and water, significantly threatening ecosystems. Monitoring environmental disturbance caused by rare-earth mining is necessary to protect the ecological environment. A spatiotemporal remote sensing monitoring method for mining to reclamation processes in a rare-earth mining area using multisource time-series satellite images is ... Read more >>

Environ Sci Pollut Res Int (Environmental science and pollution research international)
[2021, :]

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Landslide susceptibility assessment and mapping using statistical and data mining models in Iran

Abdulaziz Hanifinia, Habib Nazarnejad, Saeed Najafi, Aiding Kornejady, Hamid Reza Pourghasemi,

This study applied to evaluate landslide susceptibility using four data mining models including, “Generalized Linear Model (GLM)”, “Maximum Entropy (ME)”, “Artificial Neural Network (ANN)”, and “Support Vector Machine (SVM)” in Cherikabad Watershed in Urmia City, Iran. In particular, Shannon entropy was used to assess the intercomparison of factors’ classes. Eleven ... Read more >>

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Effects of Vegetation Restoration on Soil Enzyme Activity in Copper and Coal Mining Areas.

Hui Sun, Jianfeng Zhang, Rongjia Wang, Zongtai Li, Shiyong Sun, Guanghua Qin, Yumin Song,

Mining areas are suffering from serious environmental hazards, such as soil erosion, water pollution as well as land degradation. In this study, two types of mining areas in Anhui Province, China-one a copper mining area and the other a coal mining area-were selected to compare the soil properties under different ... Read more >>

Environ Manage (Environmental management)
[2021, :]

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Contamination Level, Source Identification And Health Risk Assessment of Potentially Toxic Elements In Drinking Water Sources of Mining And Non-Mining Areas of Khyber Pakhtunkhwa, Pakistan

Zahid Bhatti, Muhammad Ishtiaq, Said Khan, javed nawab, Sardar Khan, Shams Baig, Ihsan Muhammad, Zia Din, Asad Khan, Junaid Ghani,

Accelerated mining activities have increased water contamination with potentially toxic elements (PTEs) and their associated human health risk in developing countries. The current study investigated the distribution of PTEs, their potential sources and health risk assessment in both ground and surface water sources in mining and non–mining areas of Khyber ... Read more >>

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