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Journal PLoS One

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Insights into cell wall disintegration of Chlorella vulgaris.

Sophie Weber, Philipp M Grande, Lars M Blank, Holger Klose,

With their ability of CO2 fixation using sunlight as an energy source, algae and especially microalgae are moving into the focus for the production of proteins and other valuable compounds. However, the valorization of algal biomass depends on the effective disruption of the recalcitrant microalgal cell wall. Especially cell walls ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262500]

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Impact of Coronavirus Infectious Disease (COVID-19) pandemic on willingness of immunization-A community-based questionnaire study.

Pei-Yun Chen, Pei-Ni Chuang, Chien-Hsieh Chiang, Hao-Hsiang Chang, Chia-Wen Lu, Kuo-Chin Huang,

<h4>Background</h4>Coronavirus infectious disease 2019 (COVID-19) has had a great impact on global health, but with relatively few confirmed cases in Taiwan. People in Taiwan showed excellent cooperation with the government for disease prevention and faced social and behavioral changes during this period. This study aimed to investigate people's knowledge of ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262660]

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Evaluation of a parasite-density based pooled targeted amplicon deep sequencing (TADS) method for molecular surveillance of Plasmodium falciparum drug resistance genes in Haiti.

Swarnali Louha, Camelia Herman, Mansi Gupta, Dhruviben Patel, Julia Kelley, Je-Hoon M Oh, Janani Guru, Jean F Lemoine, Michelle A Chang, Udhayakumar Venkatachalam, Eric Rogier, Eldin Talundzic,

Sequencing large numbers of individual samples is often needed for countrywide antimalarial drug resistance surveillance. Pooling DNA from several individual samples is an alternative cost and time saving approach for providing allele frequency (AF) estimates at a population level. Using 100 individual patient DNA samples of dried blood spots from ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262616]

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Is overwintering mortality driving enigmatic declines? Evaluating the impacts of trematodes and the amphibian chytrid fungus on an anuran from hatching through overwintering.

Olivia Wetsch, Miranda Strasburg, Jessica McQuigg, Michelle D Boone,

Emerging infectious diseases are increasing globally and are an additional challenge to species dealing with native parasites and pathogens. Therefore, understanding the combined effects of infectious agents on hosts is important for species' conservation and population management. Amphibians are hosts to many parasites and pathogens, including endemic trematode flatworms (e.g., ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262561]

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Observational study of changes in utilization and outcomes in mechanical ventilation in COVID-19.

Christian Karagiannidis, Corinna Hentschker, Michael Westhoff, Steffen Weber-Carstens, Uwe Janssens, Stefan Kluge, Michael Pfeifer, Claudia Spies, Tobias Welte, Rolf Rossaint, Carina Mostert, Wolfram Windisch,

<h4>Background</h4>The role of non-invasive ventilation (NIV) in severe COVID-19 remains a matter of debate. Therefore, the utilization and outcome of NIV in COVID-19 in an unbiased cohort was determined.<h4>Aim</h4>The aim was to provide a detailed account of hospitalized COVID-19 patients requiring non-invasive ventilation during their hospital stay. Furthermore, differences of ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262315]

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Is there no "I" in team? Potential bias in key informant interviews when asking individuals to represent a collective perspective.

Whitney Fleming, Brittany King, Kerrick Robinson, Eric Wade, Brian Erickson, Jackie Delie, Ricardo de Ycaza, David Trimbach, Ana Spalding, Kelly Biedenweg,

This paper sought to understand the extent to which, and how individuals use personal or collective language when asked to articulate sense of place from a collective perspective. Understanding a collective sense of place could illuminate place-based connections in natural resource industries, where it is as groups or as institutions ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0261452]

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Politics is making us sick: The negative impact of political engagement on public health during the Trump administration.

Kevin B Smith,

<h4>Objectives</h4>To quantify the effect of politics on the physical, psychological, and social health of American adults during the four-year span of the Trump administration.<h4>Methods</h4>A previously validated politics and health scale was used to compare health markers in nationally representative surveys administered to separate samples in March 2017 (N = 800) ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262022]

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Real-world assessment and treatment of locally advanced basal cell carcinoma: Findings from the RegiSONIC disease registry.

Aleksandar Sekulic, Simon Yoo, Ragini Kudchadkar, Julie Guillen, Gary Rogers, Anne Lynn S Chang, Scott Guenthner, Bernard Raskin, Keith Dawson, Yong Mun, Laura Chu, Edward McKenna, Mario Lacouture,

<h4>Background</h4>Limited information is available regarding real-world treatment patterns and their effectiveness and safety in patients with locally advanced basal cell carcinoma, including patients not typically represented in clinical trials. The purpose of the current study was to describe how clinicians diagnose and treat locally advanced basal cell carcinoma in the ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262151]

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Healthcare utilization and costs among high-need and frail Mexican American Medicare beneficiaries.

Maricruz Rivera-Hernandez, Amit Kumar, Lin-Na Chou, Tamra Keeney, Nasim Ferdows, Amol Karmarkar, Kyriakos S Markides, Kenneth Ottenbacher,

<h4>Objectives</h4>To examine Medicare health care spending and health services utilization among high-need population segments in older Mexican Americans, and to examine the association of frailty on health care spending and utilization.<h4>Methods</h4>Retrospective cohort study of the innovative linkage of Medicare data with the Hispanic Established Populations for the Epidemiologic Study of ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262079]

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Financial vulnerability and the impact of COVID-19 on American households.

Carol Bruce, Maeve E Gearing, Jill DeMatteis, Kerry Levin, Timothy Mulcahy, Jocelyn Newsome, Jonathan Wivagg,

In May 2020, Westat, in partnership with Stanford University School of Medicine, conducted a nationally-representative household survey of American attitudes and behaviors regarding COVID-19. In this article, we examine what the Coronavirus Attitudes and Behaviors Survey tells us about the impact of COVID-19 on financial status and how this impact ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262301]

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Review on generic methods for mechanical modeling, simulation and control of soft robots.

Pierre Schegg, Christian Duriez,

In this review paper, we are interested in the models and algorithms that allow generic simulation and control of a soft robot. First, we start with a quick overview of modeling approaches for soft robots and available methods for calculating the mechanical compliance, and in particular numerical methods, like real-time ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0251059]

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The impact of cardiopulmonary exercise-derived scoring on prediction of cardio-cerebral outcome in hypertrophic cardiomyopathy.

Jae-Man Lee, Hyun-Bin Park, Jin-Eun Song, In-Cheol Kim, Ji-Hun Song, Hyungseop Kim, Jaewon Oh, Jong-Chan Youn, Geu-Ru Hong, Seok-Min Kang,

<h4>Background</h4>Sudden cardiac death (SCD) and stroke-related events accompanied by atrial fibrillation (AF) can affect morbidity and mortality in hypertrophic cardiomyopathy (HCM). This study sought to evaluate a scoring system predicting cardio-cerebral events in HCM patients using cardiopulmonary exercise testing (CPET).<h4>Methods</h4>We investigated the role of a previous prediction model based on ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0259638]

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Perinatal asphyxia and associated factors among neonates admitted to a specialized public hospital in South Central Ethiopia: A retrospective cross-sectional study.

Seifu Awgchew Mamo, Girum Sebsibie Teshome, Tewodros Tesfaye, Abel Tibebu Goshu,

<h4>Introduction</h4>Perinatal asphyxia continues to be a significant clinical concern around the world as the consequences can be devastating. World Health Organization data indicates perinatal asphyxia is encountered amongst 6-10 newborns per 1000 live full-term birth, and the figures are higher for low and middle-income countries. Nevertheless, studies on the prevalence ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262619]

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The dynamics of mitochondrial-linked gene expression among tissues and life stages in two contrasting strains of laying hens.

Clara Dreyling, Martin Hasselmann,

The cellular energy metabolism is one of the most conserved processes, as it is present in all living organisms. Mitochondria are providing the eukaryotic cell with energy and thus their genome and gene expression has been of broad interest for a long time. Mitochondrial gene expression changes under different conditions ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262613]

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Prevalence and antimicrobial resistance pattern of Clostridium difficile among hospitalized diarrheal patients: A systematic review and meta-analysis.

Tebelay Dilnessa, Alem Getaneh, Workagegnehu Hailu, Feleke Moges, Baye Gelaw,

<h4>Background</h4>Clostridium difficile is the leading cause of infectious diarrhea that develops in patients after hospitalization during antibiotic administration. It has also become a big issue in community-acquired diarrhea. The emergence of hypervirulent strains of C. difficile poses a major problem in hospital-associated diarrhea outbreaks and it is difficult to treat. ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262597]

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Risk predictive tools of perioperative drug hypersensitivity reaction: A case-control study.

Ujal Pradhan, Maliwan Oofuvong, Orarat Karnjanawanichkul, Jatuporn Pakpirom,

<h4>Objective</h4>We aimed to determine the risk factors of perioperative drug hypersensitivity reaction (DHR) and develop a predictive score for use in clinical practice.<h4>Methods</h4>A case-control study was conducted in patients who underwent anesthesia at a tertiary hospital in Thailand, between 2015-2018. DHR cases were graded clinically from 1 to 4 according ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262362]

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Comparing the timeliness and adequacy of antenatal care uptake between women who married as child brides and adult brides in 20 sub-Saharan African countries.

Sunday A Adedini, Sunday Matthew Abatan, Adesoji Dunsin Ogunsakin, Christiana Alake Alex-Ojei, Blessing Iretioluwa Babalola, Sarafa Babatunde Shittu, Emmanuel Kolawole Odusina, Lorretta Favour C Ntoimo,

<h4>Context</h4>Considering the persistent poor maternal and child health outcomes in sub-Saharan Africa (SSA), this study undertook a comparative analysis of the timing and adequacy of antenatal care uptake between women (aged 20-24 years) who married before age 18 and those who married at age 18 or above.<h4>Method</h4>Data came from Demographic ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262688]

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Efficacy of Fluorecare SARS-CoV-2 Spike Protein Test Kit for SARS-CoV-2 detection in nasopharyngeal samples of 121 individuals working in a manufacturing company.

Valentina Tonelotto, Annamaria Davini, Laura Cardarelli, Milena Calderone, Paola Marin,

<h4>Objectives</h4>The aim of this study was to evaluate the clinical performance of the Fluorecare SARS-CoV-2 Spike Protein Test Kit, a rapid immunochromatographic assay for SARS-CoV-2 detection. Moreover, we sought to point out the strategy adopted by a local company to lift the lockdown without leading to an increase in the ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262174]

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Th17 cell-mediated immune response in a subpopulation of dogs with idiopathic epilepsy.

Anna Knebel, Annika Kämpe, Regina Carlson, Karl Rohn, Andrea Tipold,

<h4>Background</h4>Canine idiopathic epilepsy (IE) is a common neurological disease with severe impact on the owner´s and the dog's quality of life. A subpopulation of dogs with IE does not respond to antiseizure drugs (non-responder). Th17 cells (T helper cells) and their proinflammatory Interleukin-17 (IL-17) are part of the immune system ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262285]

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Effect of dietary supplementation of lauric acid on growth performance, antioxidative capacity, intestinal development and gut microbiota on black sea bream (Acanthopagrus schlegelii).

Sami Ullah, Jinzhi Zhang, Bingying Xu, Arnaud Fabrice Tegomo, Gladstone Sagada, Lu Zheng, Lei Wang, Qingjun Shao,

A feeding trial of eight weeks was conducted to examine the influence of food supplementation with lauric acid (LA) on Acanthopagrus schlegelii (juvenile black sea bream). A 24 percent fish meal baseline diet was created, while the other two diets were generated with dietary supplementation of graded points of LA ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262427]

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Baseline procalcitonin as a predictor of bacterial infection and clinical outcomes in COVID-19: A case-control study.

Natalie J Atallah, Hailey M Warren, Matthew B Roberts, Ramy H Elshaboury, Monique R Bidell, Ronak G Gandhi, Meagan Adamsick, Maryam K Ibrahim, Rupali Sood, Savo Bou Zein Eddine, Matthew J Cobler-Lichter, Natalie J Alexander, Kyle D Timmer, Christine J Atallah, Adam L Viens, Vahe S Panossian, Allison K Scherer, Teddie Proctor, Sherrie Smartt, Alyssa R Letourneau, Molly L Paras, Sascha Johannes, Jan Wiemer, Michael K Mansour,

<h4>Purpose</h4>Coronavirus disease-2019 (COVID-19) is associated with a wide spectrum of clinical symptoms including acute respiratory failure. Biomarkers that can predict outcomes in patients with COVID-19 can assist with patient management. The aim of this study is to evaluate whether procalcitonin (PCT) can predict clinical outcome and bacterial superinfection in patients ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262342]

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Critical factors that affect the functioning of a research and evaluation capacity building partnership: A causal loop diagram.

Rochelle Tobin, Gemma Crawford, Jonathan Hallett, Bruce Richard Maycock, Roanna Lobo,

<h4>Introduction</h4>Public health policy and practice is strengthened by the application of quality evidence to decision making. However, there is limited understanding of how initiatives that support the generation and use of evidence in public health are operationalised. This study examines factors that support the internal functioning of a partnership, the ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0262125]

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A comparative study of adult and adolescent maternal care continuum following community-oriented interventions in Cambodia, Guatemala, Kenya, and Zambia.

Anbrasi Edward, Younghee Jung, Grace Ettyang, Chhea Chhorvann, Casey Risko, Annette E Ghee, Jane Chege,

<h4>Background</h4>The coverage for reproductive care continuum is a growing concern for communities in low- income economies. Adolescents (15-19 years) are often at higher odds of maternal morbidity and mortality due to other underlying factors including biological immaturity, social, and economic differences. The aim of the study was to examine a) ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0261161]

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"Facilitating HIV status adjustment: Qualitative insights from the Tambua Mapema proof-of-concept study in Kenya".

Elise M van der Elst, Mitchelle Abuna, Clara Agutu, Fred Ogada, Aisha Galole, Joyce Shikuku, Tony Oduor, Susan M Graham, Eduard J Sanders, Don Operario,

Systematic efforts are needed to prepare persons newly diagnosed with acute or chronic HIV infection to cope. We examined how patients dealt with this news, looking at how readiness to accept an HIV diagnosis impacted treatment outcomes, prevention of transmission, and HIV status disclosure. We examined vulnerability and agency over ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0261255]

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Automated selection of mid-height intervertebral disc slice in traverse lumbar spine MRI using a combination of deep learning feature and machine learning classifier.

Friska Natalia, Julio Christian Young, Nunik Afriliana, Hira Meidia, Reyhan Eddy Yunus, Sud Sudirman,

Abnormalities and defects that can cause lumbar spinal stenosis often occur in the Intervertebral Disc (IVD) of the patient's lumbar spine. Their automatic detection and classification require an application of an image analysis algorithm on suitable images, such as mid-sagittal images or traverse mid-height intervertebral disc slices, as inputs. Hence ... Read more >>

PLoS One (PloS one)
[2022, 17(1):e0261659]

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