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Journal PLoS One

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Development of a program for in silico optimized selection of oligonucleotide-based molecular barcodes.

In Seok Yang, Sang Won Bae, BeumJin Park, Sangwoo Kim,

Short DNA oligonucleotides (~4 mer) have been used to index samples from different sources, such as in multiplex sequencing. Presently, longer oligonucleotides (8-12 mer) are being used as molecular barcodes with which to distinguish among raw DNA molecules in many high-tech sequence analyses, including low-frequent mutation detection, quantitative transcriptome analysis, ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246354]

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LncRNAs Landscape in the patients of primary gout by microarray analysis.

Yu-Feng Qing, Jian-Xiong Zheng, Yi-Ping Tang, Fei Dai, Zeng-Rong Dong, Quan-Bo Zhang,

To determine the expression profile and clinical significance of long non-coding RNAs (lncRNAs) in peripheral blood mononuclear cells (PBMCs) of patients with primary gout and healthy control subjects. Human lncRNA microarrays were used to identify the differentially expressed lncRNAs and mRNAs in primary gout patients (n = 6) and healthy ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0232918]

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Nutritional status and effective verbal communication in Peruvian children: A secondary analysis of the 2019 Demographic and Health Survey.

Akram Hernández-Vásquez, Nilthon Pisfil-Benites, Rodrigo Vargas-Fernández, Diego Azañedo,

<h4>Background</h4>To estimate the impact of stunting on the development of effective verbal communication (EVC) in children aged 24 to 36 months.<h4>Methods</h4>We conducted a retrospective, quasi-experimental study design using data from 4452 children between 24 and 36 months of age available in the Early Childhood Development (ECD) section of the Demographic ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246542]

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Correction: Caring for children with neurodevelopmental disability: Experiences from caretakers and health workers in rural eastern Uganda.

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[This corrects the article DOI: 10.1371/journal.pone.0236488.]. ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247468]

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Assessment of muscular tone of the tongue using a digital measure spoon in a healthy population: A pilot study.

Laura Rodríguez-Alcalá, Juan Martín-Lagos Martínez, Carlos O Connor-Reina, Guillermo Plaza,

The study of the muscles of the tongue forms part of a basic evaluation of upper airway function that includes swallowing, speaking and chewing. It is important because the upper airway presents a region of collapse during sleep. Through the action of the dilator muscles, mainly the genioglossus, such collapse ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0245901]

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Interventions to reduce social isolation and loneliness during COVID-19 physical distancing measures: A rapid systematic review.

Christopher Y K Williams, Adam T Townson, Milan Kapur, Alice F Ferreira, Rebecca Nunn, Julieta Galante, Veronica Phillips, Sarah Gentry, Juliet A Usher-Smith,

<h4>Background</h4>A significant proportion of the worldwide population is at risk of social isolation and loneliness as a result of the COVID-19 pandemic. We aimed to identify effective interventions to reduce social isolation and loneliness that are compatible with COVID-19 shielding and social distancing measures.<h4>Methods and findings</h4>In this rapid systematic review, ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247139]

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In the eye of a quiet storm: A critical incident study on the quarantine experience during the coronavirus pandemic.

Ilaria Durosini, Stefano Triberti, Lucrezia Savioni, Gabriella Pravettoni,

<h4>Objective</h4>In 2020, the COVID-19 appeared in Italy with an exponential transmission capacity and serious consequences for the whole population. To counter the spread of the virus, the Italian government has adopted an extensive lockdown, forcing citizens to stay at home and avoid social contact. The COVID-19 quarantine represents a unique ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247121]

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Maternal effects on reproduction in the precocial European hare (Lepus europaeus).

Stéphanie C Schai-Braun, Peter Steiger, Thomas Ruf, Walter Arnold, Klaus Hackländer,

In female mammals, reproduction, and in particular lactation, is the energetically most exigent life-history phase. Reproduction is strongly controlled by body reserves and food availability, so females with better body condition or food supply are believed to have higher reproductive output. Additionally, the growth and mortality of young mammals depends ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247174]

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Phytochemical characterization of turnip greens (Brassica rapa ssp. rapa): A systematic review.

Gordana M Dejanovic, Eralda Asllanaj, Magda Gamba, Peter Francis Raguindin, Oche Adam Itodo, Beatrice Minder, Weston Bussler, Brandon Metzger, Taulant Muka, Marija Glisic, Hua Kern,

<h4>Objective</h4>The Turnip (Brassica rapa L. ssp. rapa) is a leaf and root vegetable grown and consumed worldwide. The consumption of Turnip has been associated with beneficial effects on human health due to their phytochemicals that may control a variety of physiological functions, including antioxidant activity, enzyme regulation, and apoptotic control ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247032]

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Cell carbon content and biomass assessments of dinoflagellates and diatoms in the oceanic ecosystem of the Southern Gulf of Mexico.

Lorena Linacre, Citlalli Sánchez-Robles, Uriel Mirabal-Gómez, J Rubén Lara-Lara, Carmen Bazán-Guzmán,

This study assessed the cell carbon content and biomass for genera of dinoflagellates and diatoms in the oceanic ecosystem of the Southern Gulf of Mexico. Carbon content estimates were based on biovolume calculations derived from linear dimension measurements of individual cells and the approximate geometric body shape of each genus. ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247071]

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Higher levels of autistic traits associated with lower levels of self-efficacy and wellbeing for performing arts professionals.

Eleanor Buckley, Elizabeth Pellicano, Anna Remington,

This study sought for the first time to identify the extent to which autistic people are pursuing careers in the performing arts, and to determine the nature of the relationship between individuals' autistic traits and their reported wellbeing. To address these aims, we recruited a self-selecting, community-based sample of individuals ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246423]

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Correction: Investigation of the relationship between sensorineural hearing loss and associated comorbidities in patients with chronic kidney disease: A nationwide, population-based cohort study.

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[This corrects the article DOI: 10.1371/journal.pone.0238913.]. ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247371]

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Direct healthcare costs of lip, oral cavity and oropharyngeal cancer in Brazil.

Vanessa Milani, Ana Laura de Sene Amâncio Zara, Everton Nunes da Silva, Larissa Barbosa Cardoso, Maria Paula Curado, Rejane Faria Ribeiro-Rotta,

The efficiency of public policies includes the measurement of the health resources used and their associated costs. There is a lack of studies evaluating the economic impact of oral cancer (OC). This study aims to estimate the healthcare costs of OC in Brazil from 2008 to 2016. This is a ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246475]

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Age-related changes in the neuromuscular control of forward and backward locomotion.

Arthur H Dewolf, Francesca Sylos-Labini, Germana Cappellini, Yury Ivanenko, Francesco Lacquaniti,

Previous studies found significant modification in spatiotemporal parameters of backward walking in healthy older adults, but the age-related changes in the neuromuscular control have been considered to a lesser extent. The present study compared the intersegmental coordination, muscle activity and corresponding modifications of spinal montoneuronal output during both forward and ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246372]

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Resilience and livestock adaptations to demographic growth and technological change: A diachronic perspective from the Late Bronze Age to Late Antiquity in NE Iberia.

Ariadna Nieto Espinet, Thomas Huet, Angela Trentacoste, Silvia Guimarães, Hector Orengo, Silvia Valenzuela-Lamas,

There are strong interactions between an economic system and its ecological context. In this sense, livestock have been an integral part of human economies since the Neolithic, contributing significantly to the creation and maintenance of agricultural anthropized landscapes. For this reason, in the frame of the ERC-StG project 'ZooMWest' we ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246201]

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Effect of nicotine exposure on the rate of orthodontic tooth movement: A meta-analysis based on animal studies.

Sanjay Jyothish, Athanasios E Athanasiou, Miltiadis A Makrygiannakis, Eleftherios G Kaklamanos,

<h4>Background</h4>Nicotine exposure has been reported to modify bone cell function and the osseous metabolism with potential effects on the rate of orthodontic tooth movement.<h4>Objectives</h4>To systematically investigate and quantitively synthesize the most recent available evidence from animal studies regarding the effect of nicotine exposure on the rate of orthodontic tooth movement.<h4>Search ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247011]

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Estimating spatial variation in the effects of climate change on the net primary production of Japanese cedar plantations based on modeled carbon dynamics.

Jumpei Toriyama, Shoji Hashimoto, Yoko Osone, Naoyuki Yamashita, Tatsuya Tsurita, Takanori Shimizu, Taku M Saitoh, Shinji Sawano, Aleksi Lehtonen, Shigehiro Ishizuka,

Spatiotemporal prediction of the response of planted forests to a changing climate is increasingly important for the sustainable management of forest ecosystems. In this study, we present a methodology for estimating spatially varying productivity in a planted forest and changes in productivity with a changing climate in Japan, with a ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247165]

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Genetic diversity of Ethiopian durum wheat landraces.

Kefyalew Negisho, Surafel Shibru, Klaus Pillen, Frank Ordon, Gwendolin Wehner,

Genetic diversity and population structure assessment in crops is essential for marker trait association, marker assisted breeding and crop germplasm conservation. We analyzed a set of 285 durum wheat accessions comprising 215 Ethiopian durum wheat landraces, 10 released durum wheat varieties, 10 advanced durum wheat lines from Ethiopia, and 50 ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247016]

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Developing an enhanced 7-color multiplex IHC protocol to dissect immune infiltration in human cancers.

Zhaoyu Sun, Richard Nyberg, Yaping Wu, Brady Bernard, William L Redmond,

The TSA Opal multiplex immunohistochemistry (mIHC) protocol (PerkinElmer) has been used to characterize immune infiltration in human cancers. This technique allows multiple biomarkers to be simultaneously stained in a single tissue section, which helps to elucidate the spatial relationship among individual cell types. We developed and optimized two improved mIHC ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247238]

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Primary and secondary supportive partnerships among HIV-positive and HIV-negative middle-aged and older gay men.

Matthew Statz, Deanna Ware, Nicholas Perry, David Huebner, Christopher Cox, Andre Brown, Steven Meanley, Sabina Haberlen, James Egan, Mark Brennan, Linda A Teplin, Robert Bolan, M Reuel Friedman, Michael Plankey,

This study describes the primary and secondary partnerships of aging gay men participating in the Understanding Patterns of Healthy Aging Among Men Who Have Sex with Men substudy of the Multicenter AIDS Cohort Study and examines differences in the prevalence of these relationship structures by HIV status while adjusting for ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0245863]

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Mapping major SARS-CoV-2 drug targets and assessment of druggability using computational fragment screening: Identification of an allosteric small-molecule binding site on the Nsp13 helicase.

Matthew R Freidel, Roger S Armen,

The 2019 emergence of, SARS-CoV-2 has tragically taken an immense toll on human life and far reaching impacts on society. There is a need to identify effective antivirals with diverse mechanisms of action in order to accelerate preclinical development. This study focused on five of the most established drug target ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246181]

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Serum symmetric dimethylarginine shows a relatively consistent long-term concentration in healthy dogs with a significant effect of increased body fat percentage.

Amber Hillaert, Daisy J X Liu, Sylvie Daminet, Bart J G Broeckx, Emmelie Stock, Dominique Paepe, Myriam Hesta, Katrien Vanderperren,

Symmetric dimethylarginine (SDMA) is a promising renal marker that correlates well with the glomerular filtration rate and could allow earlier detection of impaired renal function. The main objectives of this study were to assess the long-term variability of SDMA in healthy dogs and examine the influence of an increased body ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0247049]

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"Yes, I'm interested in taking PrEP!": PrEP interest among women respondents to the European community-based survey "Flash! PrEP in Europe".

Rosemary M Delabre, Adeline Bernier, Flor Sánchez, Antoine Vilotitch, Sophocles Chanos, Maria Luisa Cosmaro, Harriet Langanke, Coline Mey, Cary James, Sascha B Duken, Vincent Schlegel, Richard Stranz, Kai J Jonas, Bruno Spire, Daniela Rojas Castro,

The World Health Organization recommends pre-exposure prophylaxis (PrEP) for all populations at substantial risk of HIV infection, including women. However, data regarding PrEP interest among women is lacking, particularly in Europe. Factors associated with interest in using PrEP were assessed among women respondents to the Flash! PrEP in Europe (FPIE) ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246037]

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Improving the state-of-the-art in Thai semantic similarity using distributional semantics and ontological information.

Ponrudee Netisopakul, Gerhard Wohlgenannt, Aleksei Pulich, Zar Zar Hlaing,

Research into semantic similarity has a long history in lexical semantics, and it has applications in many natural language processing (NLP) tasks like word sense disambiguation or machine translation. The task of calculating semantic similarity is usually presented in the form of datasets which contain word pairs and a human-assigned ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246751]

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Novel health system strategies for tuberculin skin testing at primary care clinics: Performance assessment and health economic evaluation.

Eva Van Ginderdeuren, Jean Bassett, Colleen F Hanrahan, Lillian Mutunga, Annelies Van Rie,

<h4>Background</h4>Tuberculin skin test (TST) for guiding initiation of tuberculosis preventive therapy poses major challenges in high tuberculosis burden settings.<h4>Methods</h4>At a primary care clinic in Johannesburg, South Africa, 278 HIV-positive adults self-read their TST by reporting if they felt a bump (any induration) at the TST placement site. TST reading (in ... Read more >>

PLoS One (PloS one)
[2021, 16(2):e0246523]

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