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Journal Microb Biotechnol

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Microbes in food biotechnology: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology.

Lawrence P Wackett,

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):605-606]

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Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.

Wentao Zheng, Xue Wang, Haibo Zhou, Youming Zhang, Aiying Li, Xiaoying Bian,

The Burkholderiales are an emerging source of bioactive natural products. Their genomes contain a large number of cryptic biosynthetic gene clusters (BGCs), indicating great potential for novel structures. However, the lack of genetic tools for the most of Burkholderiales strains restricts the mining of these cryptic BGCs. We previously discovered ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):397-405]

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Bioarchaeology: a profitable dialogue between microbiology and archaeology.

Harald Brüssow,

The cultivation of yeasts from up to 5000-year-old beer vessels in Israel allows insights into early domestication of microbes for food production, but also raises questions about long-term survival of microbes under dormancy or slow growth. ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):406-409]

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An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida.

Christos Batianis, Ekaterina Kozaeva, Stamatios G Damalas, María Martín-Pascual, Daniel C Volke, Pablo I Nikel, Vitor A P Martins Dos Santos,

Owing to its wide metabolic versatility and physiological robustness, together with amenability to genetic manipulations and high resistance to stressful conditions, Pseudomonas putida is increasingly becoming the organism of choice for a range of applications in both industrial and environmental applications. However, a range of applied synthetic biology and metabolic ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):368-385]

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Specific pathogens and microbial abundance within liver and kidney tissues of wild marine fish from the Eastern Mediterranean Sea.

Dalit Meron, Nadav Davidovich, Maya Ofek-Lalzar, Ran Berzak, Aviad Scheinin, Yael Regev, Rei Diga, Dan Tchernov, Danny Morick,

This study is an initial description and discussion of the kidney and liver microbial communities of five common fish species sampled from four sites along the Eastern Mediterranean Sea shoreline. The goals of the present study were to establish a baseline dataset of microbial communities associated with the tissues of ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, :]

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Biotransformation of 2,4-dinitrotoluene in a phototrophic co-culture of engineered Synechococcus elongatus and Pseudomonas putida.

Derek T Fedeson, Pia Saake, Patricia Calero, Pablo Iván Nikel, Daniel C Ducat,

In contrast to the current paradigm of using microbial mono-cultures in most biotechnological applications, increasing efforts are being directed towards engineering mixed-species consortia to perform functions that are difficult to programme into individual strains. In this work, we developed a synthetic microbial consortium composed of two genetically engineered microbes, a ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, :]

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The gut microbiota and its interactions with cardiovascular disease.

Hui Xu, Xiang Wang, Wenke Feng, Qi Liu, Shanshan Zhou, Quan Liu, Lu Cai,

The intestine is colonized by a considerable community of microorganisms that cohabits within the host and plays a critical role in maintaining host homeostasis. Recently, accumulating evidence has revealed that the gut microbial ecology plays a pivotal role in the occurrence and development of cardiovascular disease (CVD). Moreover, the effects ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, :]

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Production of rebaudioside D from stevioside using a UGTSL2 Asn358Phe mutant in a multi-enzyme system.

Liangliang Chen, Ruxin Cai, Jingyuan Weng, Yan Li, Honghua Jia, Kequan Chen, Ming Yan, Pingkai Ouyang,

Rebaudioside D is a sweetener from Stevia rebaudiana with superior sweetness and organoleptic properties, but its production is limited by its minute abundance in S. rebaudiana leaves. In this study, we established a multi-enzyme reaction system with S. rebaudiana UDP-glycosyltransferases UGT76G1, Solanum lycopersicum UGTSL2 and Solanum tuberosum sucrose synthase StSUS1, achieving a ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, :]

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Shewanella khirikhana sp. nov. - a shrimp pathogen isolated from a cultivation pond exhibiting early mortality syndrome.

Anuphap Prachumwat, Piyanuch Wechprasit, Jiraporn Srisala, Ruttanaporn Kriangsaksri, Timothy W Flegel, Siripong Thitamadee, Kallaya Sritunyalucksana,

Early mortality syndrome (EMS) in cultivated shrimp is of complex aetiology. One of the causes is acute hepatopancreatic necrosis disease (AHPND) caused by unique Vibrio isolates that carry two Pirvp toxin genes, but other causes of EMS remain mostly unexplained. Here, we describe the discovery of a Shewanella isolate TH2012T ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, :]

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Efficacy of faecal microbiota transplantation in Crohn's disease: a new target treatment?

Liyuan Xiang, Xiao Ding, Qianqian Li, Xia Wu, Min Dai, Chuyan Long, Zhi He, Bota Cui, Faming Zhang,

The efficacy of faecal microbiota transplantation (FMT) in Crohn's disease (CD) remains unclear due to lack of data. This study aimed to assess the value of FMT in treating CD-related clinical targets. The use of FMT for CD as a registered trial (NCT01793831) was performed between October 2012 and December ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, :]

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Dual sgRNA-directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system.

Ke Liu, Bin Sun, Hao You, Jun-Liang Tu, Xuya Yu, Peng Zhao, Jun-Wei Xu,

Ganoderma lucidum is an important medicinal mushroom in traditional Chinese medicine. However, the lack of adequate genetic tools has hindered molecular genetic research in and the genetic modification of this species. Here, we report that the presence of an intron is necessary for the efficient expression of the heterologous phosphinothricin-resistance ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):386-396]

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Controlled replication of 'Candidatus Liberibacter asiaticus' DNA in citrus leaf discs.

Elham Attaran, Anna Berim, Nabil Killiny, Haluk Beyenal, David R Gang, Anders Omsland,

'Candidatus Liberibacter asiaticus' is a fastidious bacterium and a putative agent of citrus greening disease (a.k.a., huanglongbing, HLB), a significant agricultural disease that affects citrus fruit quality and tree health. In citrus, 'Ca. L. asiaticus' is phloem limited. Lack of culture tools to study 'Ca. L. asiaticus' complicates analysis of ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, :]

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Root-tip cutting and uniconazole treatment improve the colonization rate of Tuber indicum on Pinus armandii seedlings in the greenhouse.

Xiaolin Li, Lei Ye, Xiaoping Zhang, Hao Tan, Qiang Li,

The Chinese black truffle Tuber indicum is commercially valuable. The main factors influencing the success or failure of a truffle crop include the mycorrhizal colonization rate and host plant quality. The effects of a plant growth regulator (uniconazole) and plant growth management technique (root-tip cutting) on T. indicum colonization rate and ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):535-547]

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The Home Clinic or All in a Day's Work of Dr. Fics.

Kenneth Timmis,

Microb Biotechnol (Microbial biotechnology)
[2020, 13(1):3-10]

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Shifts in reclamation management strategies shape the role of exopolysaccharide and lipopolysaccharide-producing bacteria during soil formation.

Miljenka Vuko, Barbara Cania, Cordula Vogel, Susanne Kublik, Michael Schloter, Stefanie Schulz,

Polymeric substances produced by microbes play a key role for the development of soil aggregates. Here, we investigated the dynamics of bacterial families contributing to the formation of exopolysaccharides and lipopolysaccharides, major constituents of polymeric substances, at a managed land reclamation site of a post-mining area. We collected soil samples ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):584-598]

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Progress and challenges in engineering cyanobacteria as chassis for light-driven biotechnology.

Andrew Hitchcock, C Neil Hunter, Daniel P Canniffe,

Cyanobacteria are prokaryotic phototrophs that, in addition to being excellent model organisms for studying photosynthesis, have tremendous potential for light-driven synthetic biology and biotechnology. These versatile and resilient microorganisms harness the energy of sunlight to oxidise water, generating chemical energy (ATP) and reductant (NADPH) that can be used to drive ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):363-367]

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Microbial seed treatments: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology.

Lawrence P Wackett,

Microb Biotechnol (Microbial biotechnology)
[2020, 13(1):299-300]

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The rose and the name: the unresolved debate on biotechnological terms.

Martí Domínguez, Juli Peretó, Manuel Porcar,

The largest survey on the perception of synthetic biology-related disciplines (Porcar et al., 2019,EMBO Rep 20) recently revealed that the Spanish society does not have a very positive perception of the term synthetic biology. On the other hand, the terms biotechnology and even genetic engineering received relatively higher scores. The ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):305-310]

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Bioengineered Escherichia coli Nissle 1917 for tumour-targeting therapy.

Xiaoli Yu, Changsen Lin, Jing Yu, Qingsheng Qi, Qian Wang,

Bacterial vectors, as microscopic living 'robotic factories', can be reprogrammed into microscopic living 'robotic factories', using a top-down bioengineering approach to produce and deliver anticancer agents. Most of the current research has focused on bacterial species such as Salmonella typhimurium or Clostridium novyi. However, Escherichia coli Nissle 1917 (EcN) is ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2019, :]

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An Ustilago maydis chassis for itaconic acid production without by-products.

Johanna Becker, Hamed Hosseinpour Tehrani, Marc Gauert, Jörg Mampel, Lars M Blank, Nick Wierckx,

Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild-type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by-product spectrum of U. maydis ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):350-362]

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Raman-deuterium isotope probing to study metabolic activities of single bacterial cells in human intestinal microbiota.

Yi Wang, Jiabao Xu, Lingchao Kong, Tang Liu, Lingbo Yi, Hongjuan Wang, Wei E Huang, Chunmiao Zheng,

Human intestinal microbiota is important to host health and is associated with various diseases. It is a challenge to identify the functions and metabolic activity of microorganisms at the single-cell level in gut microbial community. In this study, we applied Raman microspectroscopy and deuterium isotope probing (Raman-DIP) to quantitatively measure ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):572-583]

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Issue Information.

Microb Biotechnol (Microbial biotechnology)
[2020, 13(1):1-2]

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The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne's disease-infected animals.

Benjamin M C Swift, Nathan Meade, Elsa Sandoval Barron, Malcolm Bennett, Tania Perehenic, Valerie Hughes, Karen Stevenson, Catherine E D Rees,

Here, we describe the development of a method that exploits bacteriophage D29 as a lysis agent for efficient DNA extraction from low numbers of mycobacterial cells. This method (Actiphage® ) used in combination with PCR achieved rapid and sensitive (LOD ≤ 10 cell ml-1 ) detection and identification of viable, pathogenic mycobacteria in blood ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2019, :]

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Chemical fertilization: a short-term solution for plant productivity?

Carlos Molina-Santiago, Miguel A Matilla,

The effect of long-term chemical fertilization on plant-microorganisms and microbe-microbe interactions. ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2019, :]

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Ethyl-N-dodecanoyl-l-arginate hydrochloride combats pathogens with low-resistance generation by membrane attack and modifies gut microbiota structure.

Ting Shao, Tingting Fan, Wenshu Tang, Yanting Sun, Song Gao, Huang Chen, Zhenliang Sun, Mingyao Liu, Zhengfang Yi,

Ethyl-N-dodecanoyl-l-arginate hydrochloride (LAE, ethyl lauroyl arginate HCl) is a cationic surfactant used as a food preservative with broad-spectrum antibacterial activities. However, its resistance development, influences on gut microbiome and molecular target are unclear. In this study, bacteria were stimulated by LAE for 30 days to test the bacterial resistance. Several infected ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2019, :]

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