Steven J Cook, Berta Vidal, Oliver Hobert,
Single neuron-specific drivers are important tools for visualizing neuron anatomy, manipulating neuron activity and gene rescue experiments. We report here that genomic regions upstream of the <i>C. elegans</i> bHLH-PAS gene <i>hlh-34</i> can be used to drive gene expression exclusively in the AVH interneuron pair and not, as previously reported, the ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Belen Herce-Hagiwara, Yee Mon Thu,
Sgs1p in <i>Saccharomyces cerevisiae</i> belongs to the RecQ helicase family. Sgs1p is involved in recombination during DNA damage repair and sumoylation of Sgs1p is one mechanism by which the protein is regulated. To further understand the significance of Sgs1p sumoylation in DNA damage repair, we examined the genetic interaction between ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
The first generation of <i>C. elegans</i> GAL4 drivers for bipartite expression function less well than <i>C. elegans</i> tet ON/OFF, QF and LexA drivers. The main difference between the GAL4 drivers and the others is the absence of a flexible linker between the DNA binding and activation domain in the GAL4 ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
[This retracts the article DOI: 10.17912/micropub.biology.000407.]. ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Audrey Labarre, Gilles Tossing, Claudia Maios, James J Doyle, J Alex Parker,
Mutations in the human DNA/RNA binding protein FUS are associated with amyotrophic lateral sclerosis and frontotemporal lobar degeneration, including some aggressive and juvenile onset forms. Cytoplasmic inclusions of human FUS proteins are observed in various neurodegenerative disorders, such as Huntington's disease or spinocerebellar ataxia, suggesting that FUS proteinopathy may be ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Liu Xian, Gang Yu, Alberto P Macho,
The utilization of plant gamma-aminobutyric acid (GABA) is essential for the pathogenicity of the bacterial plant pathogen <i>Ralstonia solanacearum</i>. A knockout mutant in the GABA transaminase-encoding gene <i>gabT</i> is unable to utilize GABA as a nutrient and its ability to cause disease in plants is strongly compromised. However, the proximity ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Dillon E Sloan, Joshua N Bembenek,
To generate a non-red/green fluorescent fusion histone protein in <i>C. elegans</i>, we have generated a C-terminal mTurquoise2-tagged HIS-72 at the endogenous locus using CRISPR. We found that HIS-72::mTurquoise2 localizes in a similar pattern to the previously published HIS-72::GFP strain. ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Sangsoon Park, Jooyeon Sohn, Sujeong Kwon, Eun Ji E Kim, Yoonji Jung, Hae-Eun H Park, Sieun S Kim, Seung-Jae V Lee,
Y RNA is a conserved small non-coding RNA whose functions in aging remain unknown. Here, we sought to determine the role of <i>C. elegans</i> Y RNA homologs, CeY RNA (CeY) and stem-bulge RNAs (sbRNAs), in aging. We found that the levels of CeY and sbRNAs generally increased during aging. We ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Carlos Chávez-Pérez, Niusha Jafari, Brendan T Keenan, David M Raizen, Alex M Rohacek,
The interneurons ALA and RIS both regulate stress induced sleep in <i>C. elegans</i> but their roles in awake animal movement has been reported to differ. We describe the development of a motivated mobility-based assay that distinguishes between animals mutant for ALA function and those mutant for RIS function. ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Brandon J Berry, Tyrone O Nieves, Andrew P Wojtovich,
Mitochondria are ATP-producing organelles that also signal throughout the cell. Mitochondrial protein homeostasis is regulated through membrane potential-dependent protein import and quality control signaling. The mitochondrial unfolded protein response (UPR<sup>mt</sup>) is a specific program that responds to imbalances in nuclear and mitochondrial gene expression. Mounting evidence suggests that the electrochemical ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Brooklynne Watkins, Jennifer A Schisa,
Phase separation has emerged as a widespread process of organizing the cytoplasm of diverse eukaryotic cells. In <i>C. elegans</i> oocytes, several RNA binding proteins are condensed into germ granules called P granules. Prior studies studying the phase transitions of RNA binding proteins in response to increased temperature have suggested that ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Jennifer M Gardner, Eileen O'Toole, Sue L Jaspersen,
<i>BRR6</i> and <i>BRL1</i> are two paralogs that encode transmembrane proteins of the nuclear envelope (NE) involved in membrane fluidity and nuclear pore complex biogenesis in organisms that undergo a closed mitosis. We show that mutation of a conserved cysteine in the intralumenal domain of <i>Saccharomyces cerevisiae</i> Brr6p results in a ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Hadley C Osman, Christine A Sedore, E Grace Jackson, Elena T Battistoni, David Hall, Anna Foulger, Mark Lucanic, Max Guo, Monica Driscoll, Patrick Phillips, Gordon J Lithgow,
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Kahlan Merriman, Emily Petruccelli,
The <i>Drosophila</i> transcriptional reporter of intracellular calcium (TRIC) is a genetic tool used to measure lasting changes in neuroexcitability. Both pan-neuronal and dopaminergic cells were examined with TRIC to test the hypothesis that ethanol exposure causes lasting changes in adult brain neuroexcitability. We found little to no impact on TRIC ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Sreedevi Padmanabhan, Kaustuv Sanyal, Dharanidhar Dubey,
DNA replication in eukaryotes is initiated by the orchestrated assembly and association of initiator proteins (heterohexameric Origin Recognition Complex, ORC) on the replication origins. These functionally conserved proteins play significant roles in diverse cellular processes besides their central role in ignition of DNA replication at origins. <i>Candida albicans</i>, a major ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
George Huang, Bailey de Jesus, Alex Koh, Sara Blanco, Aubrie Rettmann, Ella DeMott, Melynda Sylvester, Cassie Ren, Carrie Meng, Skye Waterland, Anita Rhodes, Persephone Alicea, Abbey Flynn, Daniel J Dickinson, Ryan Doonan,
Streamlined, selection-based CRISPR knock-in protocols for <i>C. elegans</i> were first introduced six years ago (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). Though these selection-based approaches are powerful, one drawback has been the requirement to inject large numbers of P0 worms (~30-60 per gene target). We have found that a ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Harika Dabbara, Arielle Schultz, Seol Hee Im,
Painful diabetic neuropathy (PDN) is one of the predominant complications of diabetes that causes numbness, tingling, and extreme pain sensitivity. Understanding the mechanisms of PDN pathogenesis is important for patient treatments. Here we report <i>Drosophila</i> models of diabetes-induced mechanical nociceptive hypersensitivity. Type 2 diabetes-like conditions and loss of insulin receptor ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Hiroshi Qadota, Andres F Oberhauser, Guy M Benian,
<i>C. elegans</i> UNC-112 (kindlin) is required for muscle sarcomere assembly, and is one component of a conserved four-protein complex that associates with the cytoplasmic tail of integrin at the base of integrin adhesion complexes in muscle. The four-protein complex consists of UNC-112 (kindlin), PAT-4 (integrin linked kinase; ILK), PAT-6 (alpha-parvin), ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Yini Li, Matthew Snyder, Eleanor M Maine,
Histone modifications influence gene expression and chromosome dynamics by altering chromatin structure and recruitment of nonhistone proteins. Dimethylation of histone H3 on lysine 9 (H3K9me2) is a conserved modification often found within heterochromatin. During first meiotic prophase when homologous chromosomes undergo pairing and synapsis, immunolabeling of <i>C. elegans</i> male germ ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Terminal selector-type transcription factors are key regulators of neuronal identity and function (Hobert and Kratsios, 2019; Kratsios and Hobert, 2018). Mechanistically, terminal selectors are thought to act directly through binding at the <i>cis</i>-regulatory region of genes (termed "terminal identity genes") that encode, among others, neurotransmitter [NT] synthesis proteins, ion channels, ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Luiza N Loges, Katherine M Walstrom,
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked, recessive condition that causes intermittent jaundice or hemolytic anemia because of low NADPH levels in red blood cells. We performed steady-state enzyme kinetics with the recombinant <i>C. elegans</i> ortholog of human G6PD, GSPD-1, and two mutants containing amino acid changes found in human ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Taylor N Medwig-Kinney, Nicholas J Palmisano, David Q Matus,
The helix-loop-helix transcription factor <i>hlh-2</i> (E/Daughterless) has been shown to play an important role in regulating cell fate patterning, cell cycle, and basement membrane invasion in the context of the development of the <i>C. elegans</i> somatic gonad. Here, using CRISPR/Cas9 genome engineering, we generated a new <i>hlh-2</i> allele (<i>hlh-2(Δ-1303-702)</i>) in ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
[This corrects the article DOI: 10.17912/micropub.biology.000397.]. ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Jaime Osuna-Luque, Collin Y Ewald, Peter Meister,
The <i>gpa-4</i> promoter-driven expression is described as specific for ASIL and ASIR chemosensory neurons in the nematode <i>Caenorhabditis elegans</i>, yet this was mostly examined in adult animals. Here we used a recombination-mediated reporter to test the previously used <i>gpa-4</i> promoter expression. This reporter highlights all cells in which the <i>gpa-4</i> ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times
Gabriella M Scoca, Amy C Groth,
The genome of <i>Caenorhabditis elegans</i> contains two genes of the odd-skipped transcription factor family, <i>odd-1</i> and <i>odd-2.</i> In <i>C. elegans, odd-1</i> is expressed in the intestine. A deletion mutant (<i>tm848)</i> that removes most of the <i>odd-1</i> gene, including all three zinc fingers, has no significant effect on brood size when ... Read more >>
MicroPubl Biol (microPublication biology)
[2021, 2021:]
Cited: 0 times