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The bHLH-PAS gene hlh-34 is expressed in the AVH, not AVJ interneurons.

Steven J Cook, Berta Vidal, Oliver Hobert,

Single neuron-specific drivers are important tools for visualizing neuron anatomy, manipulating neuron activity and gene rescue experiments. We report here that genomic regions upstream of the <i>C. elegans</i> bHLH-PAS gene <i>hlh-34</i> can be used to drive gene expression exclusively in the AVH interneuron pair and not, as previously reported, the ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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SGS1-SuOff rescues the mild methylmethane sulfonate sensitivity of srs2Δ cells in Saccharomyces cerevisiae.

Belen Herce-Hagiwara, Yee Mon Thu,

Sgs1p in <i>Saccharomyces cerevisiae</i> belongs to the RecQ helicase family. Sgs1p is involved in recombination during DNA damage repair and sumoylation of Sgs1p is one mechanism by which the protein is regulated. To further understand the significance of Sgs1p sumoylation in DNA damage repair, we examined the genetic interaction between ... Read more >>

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[2021, 2021:]

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Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression.

Michael Nonet,

The first generation of <i>C. elegans</i> GAL4 drivers for bipartite expression function less well than <i>C. elegans</i> tet ON/OFF, QF and LexA drivers. The main difference between the GAL4 drivers and the others is the absence of a flexible linker between the DNA binding and activation domain in the GAL4 ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Retraction: Drosophila yakuba - Tsc1.

[This retracts the article DOI: 10.17912/micropub.biology.000407.]. ... Read more >>

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[2021, 2021:]

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A single copy transgenic mutant FUS strain reproduces age-dependent ALS phenotypes in C. elegans.

Audrey Labarre, Gilles Tossing, Claudia Maios, James J Doyle, J Alex Parker,

Mutations in the human DNA/RNA binding protein FUS are associated with amyotrophic lateral sclerosis and frontotemporal lobar degeneration, including some aggressive and juvenile onset forms. Cytoplasmic inclusions of human FUS proteins are observed in various neurodegenerative disorders, such as Huntington's disease or spinocerebellar ataxia, suggesting that FUS proteinopathy may be ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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The GABA transaminase GabT is required for full virulence of Ralstonia solanacearum in tomato.

Liu Xian, Gang Yu, Alberto P Macho,

The utilization of plant gamma-aminobutyric acid (GABA) is essential for the pathogenicity of the bacterial plant pathogen <i>Ralstonia solanacearum</i>. A knockout mutant in the GABA transaminase-encoding gene <i>gabT</i> is unable to utilize GABA as a nutrient and its ability to cause disease in plants is strongly compromised. However, the proximity ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Endogenous expression and localization of HIS-72::mTurquoise2 in C. elegans.

Dillon E Sloan, Joshua N Bembenek,

To generate a non-red/green fluorescent fusion histone protein in <i>C. elegans</i>, we have generated a C-terminal mTurquoise2-tagged HIS-72 at the endogenous locus using CRISPR. We found that HIS-72::mTurquoise2 localizes in a similar pattern to the previously published HIS-72::GFP strain. ... Read more >>

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[2021, 2021:]

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Age-dependent upregulation of Y RNAs in Caenorhabditis elegans.

Sangsoon Park, Jooyeon Sohn, Sujeong Kwon, Eun Ji E Kim, Yoonji Jung, Hae-Eun H Park, Sieun S Kim, Seung-Jae V Lee,

Y RNA is a conserved small non-coding RNA whose functions in aging remain unknown. Here, we sought to determine the role of <i>C. elegans</i> Y RNA homologs, CeY RNA (CeY) and stem-bulge RNAs (sbRNAs), in aging. We found that the levels of CeY and sbRNAs generally increased during aging. We ... Read more >>

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[2021, 2021:]

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Motivated displacement assay distinguishes ALA neuron mutants from RIS neuron mutants during recovery from heat stress in Caenorhabditis elegans.

Carlos Chávez-Pérez, Niusha Jafari, Brendan T Keenan, David M Raizen, Alex M Rohacek,

The interneurons ALA and RIS both regulate stress induced sleep in <i>C. elegans</i> but their roles in awake animal movement has been reported to differ. We describe the development of a motivated mobility-based assay that distinguishes between animals mutant for ALA function and those mutant for RIS function. ... Read more >>

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[2021, 2021:]

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Decreased Mitochondrial Membrane Potential Activates the Mitochondrial Unfolded Protein Response.

Brandon J Berry, Tyrone O Nieves, Andrew P Wojtovich,

Mitochondria are ATP-producing organelles that also signal throughout the cell. Mitochondrial protein homeostasis is regulated through membrane potential-dependent protein import and quality control signaling. The mitochondrial unfolded protein response (UPR<sup>mt</sup>) is a specific program that responds to imbalances in nuclear and mitochondrial gene expression. Mounting evidence suggests that the electrochemical ... Read more >>

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[2021, 2021:]

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Phase separation dynamics of the C. elegans PGL-1 P granule protein in oocytes are sensitive to heat stress.

Brooklynne Watkins, Jennifer A Schisa,

Phase separation has emerged as a widespread process of organizing the cytoplasm of diverse eukaryotic cells. In <i>C. elegans</i> oocytes, several RNA binding proteins are condensed into germ granules called P granules. Prior studies studying the phase transitions of RNA binding proteins in response to increased temperature have suggested that ... Read more >>

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[2021, 2021:]

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A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body.

Jennifer M Gardner, Eileen O'Toole, Sue L Jaspersen,

<i>BRR6</i> and <i>BRL1</i> are two paralogs that encode transmembrane proteins of the nuclear envelope (NE) involved in membrane fluidity and nuclear pore complex biogenesis in organisms that undergo a closed mitosis. We show that mutation of a conserved cysteine in the intralumenal domain of <i>Saccharomyces cerevisiae</i> Brr6p results in a ... Read more >>

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[2021, 2021:]

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Caenorhabditis Intervention Testing Program: the herbicide diuron does not robustly extend lifespan in nematodes.

Hadley C Osman, Christine A Sedore, E Grace Jackson, Elena T Battistoni, David Hall, Anna Foulger, Mark Lucanic, Max Guo, Monica Driscoll, Patrick Phillips, Gordon J Lithgow,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Using the Drosophila Transcriptional Reporter of Intracellular Calcium (TRIC) to examine lasting ethanol-induced changes in neuroexcitability.

Kahlan Merriman, Emily Petruccelli,

The <i>Drosophila</i> transcriptional reporter of intracellular calcium (TRIC) is a genetic tool used to measure lasting changes in neuroexcitability. Both pan-neuronal and dopaminergic cells were examined with TRIC to test the hypothesis that ethanol exposure causes lasting changes in adult brain neuroexcitability. We found little to no impact on TRIC ... Read more >>

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[2021, 2021:]

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Identification and in silico analysis of the origin recognition complex in the human fungal pathogen Candida albicans.

Sreedevi Padmanabhan, Kaustuv Sanyal, Dharanidhar Dubey,

DNA replication in eukaryotes is initiated by the orchestrated assembly and association of initiator proteins (heterohexameric Origin Recognition Complex, ORC) on the replication origins. These functionally conserved proteins play significant roles in diverse cellular processes besides their central role in ignition of DNA replication at origins. <i>Candida albicans</i>, a major ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans.

George Huang, Bailey de Jesus, Alex Koh, Sara Blanco, Aubrie Rettmann, Ella DeMott, Melynda Sylvester, Cassie Ren, Carrie Meng, Skye Waterland, Anita Rhodes, Persephone Alicea, Abbey Flynn, Daniel J Dickinson, Ryan Doonan,

Streamlined, selection-based CRISPR knock-in protocols for <i>C. elegans</i> were first introduced six years ago (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). Though these selection-based approaches are powerful, one drawback has been the requirement to inject large numbers of P0 worms (~30-60 per gene target). We have found that a ... Read more >>

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[2021, 2021:]

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Drosophila insulin receptor regulates diabetes-induced mechanical nociceptive hypersensitivity.

Harika Dabbara, Arielle Schultz, Seol Hee Im,

Painful diabetic neuropathy (PDN) is one of the predominant complications of diabetes that causes numbness, tingling, and extreme pain sensitivity. Understanding the mechanisms of PDN pathogenesis is important for patient treatments. Here we report <i>Drosophila</i> models of diabetes-induced mechanical nociceptive hypersensitivity. Type 2 diabetes-like conditions and loss of insulin receptor ... Read more >>

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[2021, 2021:]

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Missense mutation of a conserved residue in UNC-112 (kindlin) eliminates binding to PAT-4 (ILK).

Hiroshi Qadota, Andres F Oberhauser, Guy M Benian,

<i>C. elegans</i> UNC-112 (kindlin) is required for muscle sarcomere assembly, and is one component of a conserved four-protein complex that associates with the cytoplasmic tail of integrin at the base of integrin adhesion complexes in muscle. The four-protein complex consists of UNC-112 (kindlin), PAT-4 (integrin linked kinase; ILK), PAT-6 (alpha-parvin), ... Read more >>

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[2021, 2021:]

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Meiotic H3K9me2 distribution is influenced by the ALG-3 and ALG-4 pathway and by poly(U) polymerase activity.

Yini Li, Matthew Snyder, Eleanor M Maine,

Histone modifications influence gene expression and chromosome dynamics by altering chromatin structure and recruitment of nonhistone proteins. Dimethylation of histone H3 on lysine 9 (H3K9me2) is a conserved modification often found within heterochromatin. During first meiotic prophase when homologous chromosomes undergo pairing and synapsis, immunolabeling of <i>C. elegans</i> male germ ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf.

Yinan Li, Paschalis Kratsios,

Terminal selector-type transcription factors are key regulators of neuronal identity and function (Hobert and Kratsios, 2019; Kratsios and Hobert, 2018). Mechanistically, terminal selectors are thought to act directly through binding at the <i>cis</i>-regulatory region of genes (termed "terminal identity genes") that encode, among others, neurotransmitter [NT] synthesis proteins, ion channels, ... Read more >>

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[2021, 2021:]

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Modeling human glucose-6-phosphate dehydrogenase mutations using C. elegans GSPD-1.

Luiza N Loges, Katherine M Walstrom,

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked, recessive condition that causes intermittent jaundice or hemolytic anemia because of low NADPH levels in red blood cells. We performed steady-state enzyme kinetics with the recombinant <i>C. elegans</i> ortholog of human G6PD, GSPD-1, and two mutants containing amino acid changes found in human ... Read more >>

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[2021, 2021:]

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Deletion of a putative HDA-1 binding site in the hlh-2 promoter eliminates expression in C. elegans dorsal uterine cells.

Taylor N Medwig-Kinney, Nicholas J Palmisano, David Q Matus,

The helix-loop-helix transcription factor <i>hlh-2</i> (E/Daughterless) has been shown to play an important role in regulating cell fate patterning, cell cycle, and basement membrane invasion in the context of the development of the <i>C. elegans</i> somatic gonad. Here, using CRISPR/Cas9 genome engineering, we generated a new <i>hlh-2</i> allele (<i>hlh-2(Δ-1303-702)</i>) in ... Read more >>

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[2021, 2021:]

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Erratum: Transcriptional Response to a Dauer-Inducing Ascaroside Cocktail in Late L1 in C. elegans.

[This corrects the article DOI: 10.17912/micropub.biology.000397.]. ... Read more >>

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[2021, 2021:]

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Widespread gpa-4 promoter-driven expression during Caenorhabditis elegans development.

Jaime Osuna-Luque, Collin Y Ewald, Peter Meister,

The <i>gpa-4</i> promoter-driven expression is described as specific for ASIL and ASIR chemosensory neurons in the nematode <i>Caenorhabditis elegans</i>, yet this was mostly examined in adult animals. Here we used a recombination-mediated reporter to test the previously used <i>gpa-4</i> promoter expression. This reporter highlights all cells in which the <i>gpa-4</i> ... Read more >>

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[2021, 2021:]

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The odd-1(tm848) mutation has no significant effect on brood size in Caenorhabditis elegans.

Gabriella M Scoca, Amy C Groth,

The genome of <i>Caenorhabditis elegans</i> contains two genes of the odd-skipped transcription factor family, <i>odd-1</i> and <i>odd-2.</i> In <i>C. elegans, odd-1</i> is expressed in the intestine. A deletion mutant (<i>tm848)</i> that removes most of the <i>odd-1</i> gene, including all three zinc fingers, has no significant effect on brood size when ... Read more >>

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[2021, 2021:]

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