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Erratum: Involving Individuals with Developmental Language Disorder and their Parents/Carers in Research Priority Setting.

An erratum was issued for: Involving Individuals with Developmental Language Disorder and their Parents/Carers in Research Priority Setting. The tables in the Representative Results section were updated. The tables in the Representative Results section were updated from: Participant (n=42) Topic Rating Identification Assessment/ diagnosis Bilingualism Lifelong impact Provision- primary Provision- secondary ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Erratum: Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection.

An erratum was issued for: Polarization of M1 and M2 Human Monocyte-Derived Cells and Analysis with Flow Cytometry upon Mycobacterium tuberculosis Infection. Author and affiliation information was updated. The author and affiliation information was updated from: Akhirunnesa Mily1, Sadaf Kalsum1, Marco Giulio Loreti1, Rokeya Sultana Rekha2, Jagadeeswara Rao Muvva1, Magda Lourda1,3, Susanna Brighenti1 1Center ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
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Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases.

Shannon K D Robin, Marc Ansari, Chakradhara Rao S Uppugunduri,

Glutathione S-transferases (GSTs) are metabolic enzymes responsible for the elimination of endogenous or exogenous electrophilic compounds by glutathione (GSH) conjugation. In addition, GSTs are regulators of mitogen-activated protein kinases (MAPKs) involved in apoptotic pathways. Overexpression of GSTs is correlated with decreased therapeutic efficacy among patients undergoing chemotherapy with electrophilic alkylating ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes.

Perwez Alam, Bryan D Maliken, Malina J Ivey, Shannon M Jones, Onur Kanisicak,

Ex vivo culture of the adult mammalian cardiomyocytes (CMs) presents the most relevant experimental system for the in vitro study of cardiac biology. Adult mammalian CMs are terminally differentiated cells with minimal proliferative capacity. The post-mitotic state of adult CMs not only restricts cardiomyocyte cell cycle progression but also limits ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Field Identification of Matricaria chamomilla using a Portable qPCR System.

Zhengxiu Yang, Zheng Quan, Tiffany Chua, Leo Li, Yanjun Zhang, Silva Babajanian, Francesco Buongiorno, Isabella Della Noce, Lorenzo Colombo, Steven Newmaster, Tricia Chua, Peter Chang, Gary Swanson, Zhengfei Lu,

Quality control in botanical products begins with the raw material supply. Traditionally, botanical identification is performed through morphological assessment and chemical analytical methods. However, the lack of availability of botanists, especially in recent years, coupled with the need to enhance quality control to combat the stresses on the supply chain ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development using Nomarski Microscopy.

Victor Escrich, Begoña Ezcurra, Eva Gómez-Orte, Cristina Romero-Aranda, Antonio Miranda-Vizuete, Juan Cabello,

4D microscopy is an invaluable tool for unraveling the embryonic developmental process in different animals. Over the last decades, Caenorhabditis elegans has emerged as one of the best models for studying development. From an optical point of view, its size and transparent body make this nematode an ideal specimen for ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos.

Rachel A Keuls, Ronald Parchem,

MicroRNAs (miRNAs) are important for the complex regulation of cell fate decisions and developmental timing. In vivo studies of the contribution of miRNAs during early development are technically challenging due to the limiting cell number. Moreover, many approaches require a miRNA of interest to be defined in assays such as ... Read more >>

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A Standardized Method for Measurement of Elbow Kinesthesia.

Molly Watkins, Emily Duncanson, Emma Gartner, Samantha Paripovich, Christine Taylor, Alexandra Borstad,

Proprioception is an important component of controlled movement. The threshold to detection of passive movement (TDPM) is a commonly used method for quantifying the proprioceptive submodality of kinesthesia in research settings. The TDPM paradigm has been found to be valid and reliable; however, the equipment and methods used for TDPM ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy.

Nicholas R Jaegers, Wenda Hu, Yong Wang, Jian Zhi Hu,

Nuclear magnetic resonance (NMR) spectroscopy represents an important technique to understand the structure and bonding environments of molecules. There exists a drive to characterize materials under conditions relevant to the chemical process of interest. To address this, in situ high-temperature, high-pressure MAS NMR methods have been developed to enable the ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids.

Maxwell E Edmonds, Micah D Forshee, Teresa K Woodruff,

Testicular organoids provide a tool for studying testicular development, spermatogenesis, and endocrinology in vitro. Several methods have been developed in order to create testicular organoids. Many of these methods rely upon extracellular matrix (ECM) to promote de novo tissue assembly, however, there are differences between methods in terms of biomimetic ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Erratum: Using a Virtual Reality Walking Simulator to Investigate Pedestrian Behavior.

An erratum was issued for: Using a Virtual Reality Walking Simulator to Investigate Pedestrian Behavior. An affiliation was updated. The first affiliation was updated from: Department of Sports Science, Kunsan National University to: Department of Sport and Exercise Sciences, Kunsan National University. ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice.

Dawei Xu, Palash Chandra Mondol, Muhammad Uzair, Matthew R Tucker, Dabing Zhang,

Male sterility is an important agronomic trait for hybrid seed production that is usually characterized by functional defects in male reproductive organs/gametes. Recent advances in CRISPR-Cas9 genome editing technology allow for high editing efficacy and timesaving knockout mutations of endogenous candidate genes at specific sites. Additionally, Agrobacterium-mediated genetic transformation of ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses.

Juho-Antti Mäkelä, Sheyla Cisneros-Montalvo, Tiina Lehtiniemi, Opeyemi Olotu, Hue M La, Jorma Toppari, Robin M Hobbs, Martti Parvinen, Noora Kotaja,

Spermatogenesis is a unique differentiation process that ultimately gives rise to one of the most distinct cell types of the body, the sperm. Differentiation of germ cells takes place in the cytoplasmic pockets of somatic Sertoli cells that host 4 to 5 generations of germ cells simultaneously and coordinate and ... Read more >>

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Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans.

Dharini van der Hoeven, Thuy Nhu L Truong, Ali Naji, Sabita Thapa, John F Hancock, Ransome van der Hoeven,

The changes in the plasma membrane localization of the epidermal growth factor receptor (EGFR) and its downstream effector RAS have been implicated in several diseases including cancer. The free-living nematode C. elegans possesses an evolutionary and functionally conserved EGFR-RAS-ERK MAP signal cascade which is central for the development of the ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Automated Gait Analysis to Assess Functional Recovery in Rodents with Peripheral Nerve or Spinal Cord Contusion Injury.

Johannes Heinzel, Nicole Swiadek, Mohamed Ashmwe, Alexander Rührnößl, Viola Oberhauser, Jonas Kolbenschlag, David Hercher,

Peripheral and central nerve injuries are mostly studied in rodents, especially rats, given the fact that these animal models are both cost-effective and a lot of comparative data has been published in the literature. This includes a multitude of assessment methods to study functional recovery following nerve injury and repair. ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons.

David G Keener, Amy Cheung, Kensuke Futai,

Electroporation has established itself as a critical method for transferring specific genes into cells to understand their function. Here, we describe a single-cell electroporation technique that maximizes the efficiency (~80%) of in vitro gene transfection in excitatory and class-specific inhibitory neurons in mouse organotypic hippocampal slice culture. Using large glass ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4 +/15NH4 + Analyses via Sequential Conversion to N2O.

Megumi Kuroiwa, Keitaro Fukushima, Kazuma Hashimoto, Yukiko Senga, Tsubasa Sato, Chie Katsuyama, Yuichi Suwa,

The importance of understanding the fate of nitrate (NO3-), which is the dominant N species transferred from terrestrial to aquatic ecosystems, has been increasing because global nitrogen loads have dramatically increased following industrialization. Dissimilatory nitrate reduction to ammonium (DNRA) and denitrification are both microbial processes that use NO3- for respiration. ... Read more >>

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[2020, (164):]

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Isolation of Cardiomyocytes from Fixed Hearts for Immunocytochemistry and Ploidy Analysis.

Doğacan Yücel, Jacob Solinsky, Jop H van Berlo,

The adult mammalian heart is composed of various cell types including cardiomyocytes, endothelial cells and fibroblasts. Since it is difficult to reliably identify nuclei of cardiomyocytes on histological sections, many groups rely on isolating viable cardiomyocytes prior to fixation to perform immunostaining. However, these live cardiomyocyte isolation techniques require optimization ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Extracellular Multi-Unit Recording from the Olfactory Nerve of Teleosts.

Peter Hubbard, Zélia Velez,

Recent studies have shown that ocean acidification affects olfactory-driven behavior in fish. This may be due in part to a reduction in olfactory sensitivity in high PCO2/low pH water. To assess the effects of ocean acidification, or olfactory sensitivity in marine fish in general, we propose that extracellular multi-unit recording ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios.

Burkhard Hoppenstedt, Thomas Probst, Manfred Reichert, Winfried Schlee, Klaus Kammerer, Myra Spiliopoulou, Johannes Schobel, Michael Winter, Anna Felnhofer, Oswald D Kothgassner, Rüdiger Pryss,

In medicine or industry, the analysis of high-dimensional data sets is increasingly required. However, available technical solutions are often complex to use. Therefore, new approaches like immersive analytics are welcome. Immersive analytics promise to experience high-dimensional data sets in a convenient manner for various user groups and data sets. Technically, ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Erratum: Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae.

An erratum was issued for: Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae. A figure was updated. Figure 1 was updated from: Figure 1: The sucrose-yeast (SY) diets used in our protocol. (A) The blue dots represent the isocaloric (248 calories/L) macronutrient balancing diets used in the feeding ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Revised and Neuroimaging-Compatible Versions of the Dual Task Screen.

Allan M Aumen, Kelly J Oberg, Susan M Mingils, Cecelia B Berkner, Brian L Tracy, Jaclyn A Stephens,

Dual task paradigms simultaneously assess motor and cognitive abilities, and they can detect subtle, residual impairments in athletes with recent mild traumatic brain injury (mTBI). However, past dual task paradigms have focused solely on lower extremity skills and have relied on cumbersome, expensive laboratory equipment - thus limiting their practicality ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Epitaxial Nanostructured α-Quartz Films on Silicon: From the Material to New Devices.

Claire Jolly, David Sanchez-Fuentes, Ricardo Garcia-Bermejo, Dilek Cakiroglu, Adrian Carretero-Genevrier,

In this work, we show a detailed engineering route of the first piezoelectric nanostructured epitaxial quartz-based microcantilever. We will explain all the steps in the process starting from the material to the device fabrication. The epitaxial growth of α-quartz film on SOI (100) substrate starts with the preparation of a ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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An Assessment Method and Toolkit to Evaluate Keyboard Design on Smartphones.

Yincheng Wang, Ke Wang, Yuqi Huang, Di Wu, Jian Wu, Jibo He,

Keyboard input has played an essential role in human-computer interaction with a vast user base, and the keyboard design has always been one of the fundamental objects of studies on smart devices. With the development of screen technology, more precise data and indicators could be collected by smartphones to in-depth ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2020, (164):]

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Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril.

Weng-I Katherine Chio, Gemma Davison, Tabitha Jones, Jia Liu, Ivan P Parkin, Tung-Chun Lee,

This work describes a rapid and highly sensitive method for the quantitative detection of an important biomarker, uric acid (UA), via surface-enhanced Raman spectroscopy (SERS) with a low detection limit of ~0.2 μM for multiple characteristic peaks in the fingerprint region, using a modular spectrometer. This biosensing scheme is mediated ... Read more >>

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