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Review of the 2021 AAVLD Communications Survey.

Grant N Burcham, Pam Ferro, Grant Maxie,

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211043031]

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Detection of rabbit hemorrhagic disease virus 2 in formalin-fixed, paraffin-embedded tissues via in situ hybridization.

Alicia D O'Toole, Jian Zhang, Laura B A Williams, Corrie C Brown,

Formalin-fixed, paraffin-embedded tissues from European rabbits (<i>Oryctolagus cuniculus</i>) that succumbed to rabbit hemorrhagic disease virus 2 (RHDV2; <i>Lagovirus</i> GI.2) during the 2019 outbreak in Washington, USA, were utilized for in situ hybridization via RNAscope (ACDBio). This detection method was both sensitive and specific, with no staining in tissues from RHDV- ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211047561]

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Fecal PCR testing for detection of Clostridium perfringens and Clostridioides difficile toxin genes and other pathogens in foals with diarrhea: 28 cases.

K Gary Magdesian, Samantha Barnum, Nicola Pusterla,

<i>Clostridium perfringens</i> and <i>Clostridioides difficile</i> cause significant morbidity and mortality in foals. Antemortem diagnosis of <i>C. perfringens</i> infection has been complicated by a paucity of tests available for toxin detection. Fecal PCR panels have assays for a variety of <i>C. perfringens</i> toxin gene sequences as well as for several other ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211047529]

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Species-specific PCR assay for the detection of Babesia odocoilei.

Hilary J Burgess, Betty P Lockerbie, Lisanework E Ayalew, Antonia Dibernardo, Kristýna Hrazdilová, David Modry, Trent K Bollinger,

We developed a PCR assay for the detection of <i>Babesia odocoilei</i> based on the 18S rRNA gene. Multiple specimens of <i>B. odocoilei</i> were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other <i>Babesia</i> species and ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211032927]

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Validation of a commercial human ELISA to measure hyaluronic acid concentration in feline plasma.

Kaela E Shaw, Alexa M Bersenas, Shane W Bateman, Shauna L Blois, R Darren Wood,

Our goal was to validate a human hyaluronic acid (HA) ELISA (Hyaluronic acid plus ELISA; TECOmedical Group) for use in feline plasma. Plasma from 5 healthy cats and 5 critically ill cats was used for validation of the assay. Validation methods performed included intra- and inter-assay variability, spike-and-recovery, and dilutional ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211044718]

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Concurrent lacrimal gland melanocytoma and ocular melanocytosis in a dog.

Ikki Mitsui, Seigi Nishimura,

A 9-y-5-mo-old, spayed female, mixed-breed dog with buphthalmia and elevated intraocular pressure in the left eye, consistent with glaucoma, was evaluated. Black-pigmented, slightly elevated tissue with irregular margins was noted on the dorsolateral aspect of the left globe. Ultrasonography detected a mass, later identified as lacrimal gland, adjacent to the ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211045027]

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Thymoma originating from the cervical component of the thymus in a degu.

Naka Okumura, Hirotaka Kondo, Shinichi Suzuki, Hisashi Shibuya,

A 5-y-old, male degu (<i>Octodon degus</i>) was presented with a subcutaneous mass in the ventral aspect of the cervical area. The mass was removed surgically. Histologically, the mass was a densely cellular, expansile neoplasm, with compression of thymic tissue to the periphery. The neoplasm consisted of solid sheets of polygonal ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211045643]

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Adenomyomatous uterine polyp in a Miniature Pinscher: histologic, immunohistochemical, and clinical aspects.

Phawut Nueangphuet, Mika Tanabe, Uda Zahli Izzati, Naoyuki Fuke, Takuya Hirai, Ryoji Yamaguchi,

A 14-y-old Miniature Pinscher bitch was admitted to a veterinary clinic because of inappetence and a distended abdomen; ultrasound examination revealed a fluid-filled uterus with a single 1-cm hyperechoic nodule in its lumen. Ovariohysterectomy was performed. Grossly, the uterine horns were distended irregularly and asymmetrically, and the uterine lumen contained ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211043221]

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Rattlesnake envenomation in 2 Visayan warty pigs.

Eileen E Henderson, Cynthia K Stadler, Robert H Poppenga, Javier Asin Ros, Francisco A Uzal,

Rattlesnake envenomation is an important cause of morbidity and mortality in humans and animals in the southwestern United States and elsewhere. Two Visayan warty pigs (<i>Sus cebifrons</i>) from a regional zoo were submitted for autopsy after being found dead close to a southern Pacific rattlesnake (<i>Crotalus helleri</i>) in their enclosure. ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211044555]

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The effect of analytical temperature on thromboelastography tracings in dogs.

Marie T Keith, Nolan V Chalifoux, Yekaterina Buriko,

Viscoelastic testing methods such as thromboelastography (TEG) are becoming increasingly available to veterinarians in a clinical setting. TEG is useful in determining therapeutic transfusion needs and assessing global abnormalities of hemostasis of patients, given that it provides a more comprehensive assessment of coagulation than traditional tests. TEG is standardly performed ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211042922]

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Improved detection of Mycobacterium tuberculosis and M. bovis in African wildlife samples using cationic peptide decontamination and mycobacterial culture supplementation.

Wynand J Goosen, Léanie Kleynhans, Tanya J Kerr, Paul D van Helden, Peter Buss, Robin M Warren, Michele A Miller,

In South Africa, mycobacterial culture is regarded as the gold standard for the detection of <i>Mycobacterium tuberculosis</i> complex (MTBC) infection in wildlife even though it is regarded as "imperfect." We compared a novel decontamination and mycobacterial culture technique (TiKa) to the conventional mycobacterium growth indicator tube (MGIT) system using known ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211044192]

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Systemic mucoid degeneration of the arterial tunica intima in a young dog.

Yoshiki Ichikawa, Mizuki Heishima, Kristin Vyhnal, Keisuke Aoshima, Kazuyoshi Sasaoka, Ryohei Kinoshita, Atsushi Kobayashi, Mitsuyoshi Takiguchi, Takashi Kimura,

A 27-mo-old, spayed female mixed-breed dog was presented with left forelimb pain, which progressed to full thickness necrosis of the soft tissues of multiple limbs. Clinical imaging and postmortem examination suggested multiple large arterial thromboemboli. Histologic examination of vascular lesions revealed markedly thickened tunica intima with polypoid intraluminal projections, which ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211042587]

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Measurement of progesterone in sheep using a commercial ELISA kit for human plasma.

Valeria Pasciu, Maria Nieddu, Elena Baralla, Cristian Porcu, Francesca Sotgiu, Fiammetta Berlinguer,

Determination of serum or plasma progesterone (P4) concentrations is important to recognize pregnant and non-pregnant ewes, and also to predict the number of carried lambs. The 2 most common methodologies for the detection of plasma P4 are radioimmunoassay (RIA) and enzyme immunoassay (EIA). RIA is very expensive, and not all ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211043513]

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Mycobacterium fortuitum abortion in a sow.

Allysa L Cole, Natalie M Kirk, Leyi Wang, Chien-Che Hung, Jonathan P Samuelson,

Two aborted Chester White pig fetuses were presented to a veterinary diagnostic laboratory in Illinois. Postmortem examination identified no gross abnormalities. Histologic evaluation revealed multifocal necrosis of chorionic epithelial cells, coalescing areas of mineralization in the placenta, and focal accumulations of viable and degenerate neutrophils in the lung. Intra- and ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211042289]

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The comparative pathology of enterocolitis caused by Clostridium perfringens type C, Clostridioides difficile, Paeniclostridium sordellii, Salmonella enterica subspecies enterica serovar Typhimurium, and nonsteroidal anti-inflammatory drugs in horses.

Fábio S Mendonça, Mauricio A Navarro, Francisco A Uzal,

To determine if there were significant differences produced by 5 of the most prevalent causes of equine enterocolitis, we studied retrospectively the gross and microscopic pathology of 90 cases of enterocolitis submitted to the San Bernardino laboratory of the California Animal Health and Food Safety Laboratory. Included were cases caused ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211041091]

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Encephalopathy caused by Talisia esculenta intoxication in pregnant ewes and their newborn lambs.

Valdir M Almeida, Givaldo B Silva-Filho, Hisadora A S C Bom, Silvio M C Fonseca, Franklin Riet-Correa, Francisco A Uzal, Kevin D Woolard, Francisco A L Souza, Fábio S Mendonça,

An outbreak of acute encephalopathy occurred in pregnant ewes and their newborn lambs associated with consumption of <i>Talisia esculenta</i> fruits and bark. Clinical signs in 5 adult pregnant ewes included drooling, bloat, tachypnea, depression, ataxia, body shaking, difficulty in rising, and recumbency. Three neonatal lambs born to some of those ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211041098]

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Detection of pseudorabies virus antibody in swine serum and oral fluid specimens using a recombinant gE glycoprotein dual-matrix indirect ELISA.

Ting-Yu Cheng, Ronaldo Magtoto, Alexandra Henao-Díaz, Korakrit Poonsuk, Alexandra Buckley, Albert Van Geelen, Kelly Lager, Jeffrey Zimmerman, Luis Giménez-Lirola,

Pseudorabies (Aujeszky disease) virus (PRV) was eliminated from domestic swine in many countries using glycoprotein E (gE)-deleted vaccines and serum antibody gE ELISAs, but PRV continues to circulate in some regions and in most feral swine populations in the world. We created a dual-matrix (serum and oral fluid) indirect IgG ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211040755]

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Molecular characterization of Sarcocystis spp. as a cause of protozoal encephalitis in a free-ranging black bear.

Jordan B Greenfield, Madison V Anderson, Emily A Dorey, Elizabeth Redman, John S Gilleard, Nicole M Nemeth, Jamie L Rothenburger,

A free-ranging juvenile male black bear (<i>Ursus americanus</i>), found dead in Alberta, Canada, had severe nonsuppurative encephalitis. Lesions in the brain were most severe in the gray matter of the cerebral cortex, and included perivascular cuffs of lymphocytes and plasma cells, areas of gliosis that disrupted the neuropil, and intralesional ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211038389]

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A panel of real-time PCR assays for the detection of Bourbon virus, Heartland virus, West Nile virus, and Trypanosoma cruzi in major disease-transmitting vectors.

Anushri Warang, Michael Zhang, Shuping Zhang, Zhenyu Shen,

Vector-borne pathogens, such as Bourbon virus (BRBV), Heartland virus (HRTV), West Nile virus (WNV), and <i>Trypanosoma cruzi</i> (TCZ) are a great threat to public health and animal health. We developed a panel of TaqMan real-time PCR assays for pathogen surveillance. PCR targets were selected based on nucleic acid sequences deposited ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211039549]

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Using the Idexx SediVue Dx to predict the need for urine bacteriologic culture in cats.

Elisabeth Neubert, Karin Weber,

We analyzed urine samples from 191 cats for bacteriuria with an automated urine sediment analyzer (Idexx SediVue Dx), combined with image review by an observer, and compared to bacteriologic culture results. Sixty-nine samples were unambiguously assigned to be free of bacteria by the instrument and the observer, and no bacterial ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211038918]

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Effect of pooling udder skin wipes on the detection of influenza A virus in preweaning pigs.

Anne C de Lara, Jorge Garrido-Mantilla, Gustavo Lopez-Moreno, My Yang, David E S N Barcellos, Montserrat Torremorell,

Influenza A virus (IAV) active surveillance in pigs prior to weaning is commonly conducted by collecting individual samples, mostly nasal swabs. Recently, the use of udder skin wipes collected from lactating sows was identified as an effective sampling method to indicate IAV status of suckling piglets prior to weaning. However, ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211039462]

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Atrazine intoxication in cattle, confirmed by gas chromatography-mass spectrometry.

Abigail J Props, Hilary J Richards, Stephen B Hooser, Grant N Burcham, Christina R Wilson-Frank,

Ten of 40 cows died within 48 h of gaining access to a barn in which various chemicals were stored. Some of the surviving cows exhibited drooling, muscle tremors, and agitation. Postmortem examinations of 2 cows were performed in the field, and revealed nonspecific, moderate-to-severe pulmonary congestion. Liver and rumen contents, ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211036765]

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Verminous meningoencephalomyelitis in a red kangaroo associated with Angiostrongylus cantonensis infection.

Sonika Patial, Brooke A Delcambre, Peter M DiGeronimo, Gary Conboy, Adriano F Vatta, Rudy Bauer,

<i>Angiostrongylus cantonensis</i> is a zoonotic parasitic helminth that normally resides in the pulmonary arteries and the right ventricle of rats (<i>Rattus</i> sp.), the definitive host, where it causes little disease. Humans, dogs, opossums, and various zoo animals are "accidental" hosts. Here we report verminous meningoencephalomyelitis caused by <i>A. cantonensis</i> in ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211037664]

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Parvoviral enteritis and salmonellosis in raccoons with sudden death.

Chun-Ming Lin, Benjamin Hause, Deanna Gualtieri, Nicholas Robinson,

Eight of 9 juvenile raccoons at a rehabilitation center died without obvious prior clinical signs. Gross changes were unremarkable except for mildly distended intestines. Microscopically, crypt loss, distension, necrosis, and regeneration with intranuclear viral inclusions were observed in the small intestine, with marked lymphoid depletion and necrosis in Peyer patches ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211038766]

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Eumycetoma and disseminated phaeohyphomycosis in a Sumatran tiger.

Amanda R Schaff, Sylvia Ferguson, Kristen Phair, Rachel Ferris, Alexandra Goe,

A 16-y-old female Sumatran tiger (<i>Panthera tigris sumatrae</i>) was evaluated for hyporexia. Examination revealed chronic kidney disease and a large subcutaneous axillary mass with draining tracts that contained numerous small black grains. Histologic examination revealed the presence of intralesional fungal hyphae. Persistent hyporexia and pyogranulomatous disease, as well as progressive ... Read more >>

J Vet Diagn Invest (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc)
[2021, :10406387211038920]

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