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Changing the Diameter of the Bone Tunnel Is More Effective Than Changing the Tunnel Shape for Restoring Joint Functionality After ACL Reconstruction.

Huizhi Wang, Min Zhang, Cheng-Kung Cheng,

The clinical implications of changing the shape of the bone tunnel for Anterior cruciate ligament reconstruction (ACLR) is controversial and few studies have reported on the long-term prevalence for osteoarthritis. As such, this study aims to evaluate the effect of tunnel shape on joint biomechanics. Finite element models of an ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:173]

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Overcoming Energetic Barriers in Acetogenic C1 Conversion.

Alexander Katsyv, Volker Müller,

Currently one of the biggest challenges for society is to combat global warming. A solution to this global threat is the implementation of a CO<sub>2</sub>-based bioeconomy and a H<sub>2</sub>-based bioenergy economy. Anaerobic lithotrophic bacteria such as the acetogenic bacteria are key players in the global carbon and H<sub>2</sub> cycle and ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:621166]

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Methods to Scale Down Graphene Oxide Size and Size Implication in Anti-cancer Applications.

Immacolata Tufano, Raffaele Vecchione, Paolo Antonio Netti,

Despite considerable progress in the comprehension of the mechanisms involved in the origin and development of cancer, with improved diagnosis and treatment, this disease remains a major public health challenge with a considerable impact on the social and economic system, as well as on the individual. One way to improve ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:613280]

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Characteristics of Medical Products Comprising Human Cells, Genes, or Tissues Developed in Japan and the European Union Compared via Public Assessment Reports.

Ryosuke Kurauchi, Hiroi Kasai, Tatsuya Ito,

Medical products comprising human cells, genes, and tissues have been developed for clinical applications worldwide, and their developmental environment has been established. These products can be imported and exported, but marketing authorization regulations are complicated among regions. This investigation was conducted to identify the characteristics of medical products comprising human ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:606606]

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Elucidating the Role of Virulence Traits in the Survival of Pathogenic E. coli PI-7 Following Disinfection.

Krishnakumar Sivakumar, Robert Lehmann, Andri Taruna Rachmadi, Nicolas Augsburger, Noor Zaouri, Jesper Tegner, Pei-Ying Hong,

Reuse and discharge of treated wastewater can result in dissemination of microorganisms into the environment. Deployment of disinfection strategies is typically proposed as a last stage remediation effort to further inactivate viable microorganisms. In this study, we hypothesize that virulence traits, including biofilm formation, motility, siderophore, and curli production along ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:614186]

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RA-UNet: A Hybrid Deep Attention-Aware Network to Extract Liver and Tumor in CT Scans.

Qiangguo Jin, Zhaopeng Meng, Changming Sun, Hui Cui, Ran Su,

Automatic extraction of liver and tumor from CT volumes is a challenging task due to their heterogeneous and diffusive shapes. Recently, 2D deep convolutional neural networks have become popular in medical image segmentation tasks because of the utilization of large labeled datasets to learn hierarchical features. However, few studies investigate ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:605132]

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Modeled Structure of the Cell Envelope Proteinase of Lactococcus lactis.

Egon Bech Hansen, Paolo Marcatili,

The cell envelope proteinase (CEP) of <i>Lactococcus lactis</i> is a large extracellular protease covalently linked to the peptidoglycan of the cell wall. Strains of <i>L. lactis</i> are typically auxotrophic for several amino acids and in order to grow to high cell densities in milk they need an extracellular protease. The ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:613986]

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In situ Gelling Amphotericin B Nanofibers: A New Option for the Treatment of Keratomycosis.

Benedikt Göttel, Henrike Lucas, Frank Syrowatka, Wolfgang Knolle, Judith Kuntsche, Joana Heinzelmann, Arne Viestenz, Karsten Mäder,

The purpose of our research was the development of Amphotericin B-loaded <i>in situ</i> gelling nanofibers for the treatment of keratomycosis. Different formulation strategies were applied to increase the drug load of the sparingly water-soluble Amphotericin B in electrospun Gellan Gum/Pullulan fibers. These include bile salt addition, encapsulation in poly(lactic-co-glycolic acid) ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:600384]

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Renewable Cyclopentanol From Catalytic Hydrogenation-Rearrangement of Biomass Furfural Over Ruthenium-Molybdenum Bimetallic Catalysts.

Shihang Meng, Yujing Weng, Xiaolong Wang, Hongxing Yin, Zhenfei Wang, Qi Sun, Maohong Fan, Yulong Zhang,

Biomass furfural-like compounds are chemicals that cannot be extracted from fossil materials, through which a large number of fine chemicals and fuel additives can be opened up, but one big efficiency problem during the transformation is the accumulation of oligomers. Here, we propose a novel and efficient Ru-Mo bimetallic catalyst ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:615235]

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Insulin-Containing Wound Dressing Promotes Diabetic Wound Healing Through Stabilizing HIF-1α.

Peilang Yang, Di Wang, Yan Shi, Mingzhong Li, Min Gao, Tianyi Yu, Dan Liu, Jie Zhang, Jizhuang Wang, Xiong Zhang, Yan Liu,

HIF-1α is seen as a major regulator during wound healing and controls many wound healing processes, such as angiogenesis, extracellular deposition, and reepithelialization. A diabetic state plays a vicious effect on wound healing, and the destabilization of HIF-1α is a non-negligible factor. Insulin-loaded silk fibroin microparticles were prepared to release ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:592833]

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Editorial: Pathway, Genetic and Process Engineering of Microbes for Biopolymer Synthesis.

Ignacio Poblete-Castro, Bruce A Ramsay, Bernd H A Rehm,

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:618383]

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Allochthonous and Autochthonous Halothermotolerant Bioanodes From Hypersaline Sediment and Textile Wastewater: A Promising Microbial Electrochemical Process for Energy Recovery Coupled With Real Textile Wastewater Treatment.

Refka Askri, Benjamin Erable, Luc Etcheverry, Sirine Saadaoui, Mohamed Neifar, Ameur Cherif, Habib Chouchane,

The textile and clothing industry is the first manufacture sector in Tunisia in terms of employment and number of enterprises. It generates large volumes of textile dyeing wastewater (TDWW) containing high concentrations of saline, alkaline, and recalcitrant pollutants that could fuel tenacious and resilient electrochemically active microorganisms in bioanodes of ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:609446]

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L-asparaginase Production by Leucosporidium scottii in a Bench-Scale Bioreactor With Co-production of Lipids.

Ignacio S Moguel, Celina K Yamakawa, Adalberto Pessoa, Solange I Mussatto,

L-asparaginase (ASNase) is a therapeutical enzyme used for treatment of acute lymphoblastic leukemia. ASNase products available in the market are produced by bacteria and usually present allergic response and important toxicity effects to the patients. Production of ASNase by yeasts could be an alternative to overcome these problems since yeasts ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:576511]

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Charge-Mediated Co-assembly of Amphiphilic Peptide and Antibiotics Into Supramolecular Hydrogel With Antibacterial Activity.

Lei Xu, Qian Shen, Linzhuo Huang, Xiaoding Xu, Huiyan He,

Bacteria are the most common pathogens to cause infection of surgical sites, which usually induce severe postoperative morbidity and more healthcare costs. Inhibition of bacteria adhesion and colonization is an effective strategy to prevent the spread of infection at the surgical sites. Hydrogels have been widely used as promising antibacterial ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:629452]

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Effects of Astragalus Polysaccharides Nanoparticles on Cerebral Thrombosis in SD Rats.

Qian Sun, Pengqiang Shi, Cuiling Lin, Jing Ma,

<h4>Objective</h4>To investigate the efficacy and improvement of Astragalus polysaccharides (APS) and APS-nano on cerebral thrombosis in rats.<h4>Methods</h4>A total of 72 SD rats were randomly divided into NC group, Model group, APS-Nano group, and APS group. The cerebral thrombosis Model of SD rats was established by injecting compound thrombus inducer into ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:616759]

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Study on Development of Composite Hydrogels With Tunable Structures and Properties for Tumor-on-a-Chip Research.

Zaozao Chen, Fei Wang, Jie Zhang, Xiaowei Sun, Yuchuan Yan, Yan Wang, Jun Ouyang, Jing Zhang, Tess Honore, Jianjun Ge, Zhongze Gu,

A major factor for developing new tumor models is to recreate a proper three-dimensional environment for 3D tumors culture. In this 3D microenvironment, extracellular matrices play important roles in regulation of hallmark features of cancer through biochemical and mechanical signals. The fabrication of a mechanical and biophysical controllable hydrogel, while ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:611796]

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Proteomic Reveals Reasons for Acquired Drug Resistance in Lung Cancer Derived Brain Metastasis Based on a Newly Established Multi-Organ Microfluidic Chip Model.

Mingxin Xu, Yingyan Wang, Wenzhe Duan, Shengkai Xia, Song Wei, Wenwen Liu, Qi Wang,

Anti-tumor drugs can effectively shrink the lesions of primary lung cancer; however, it has limited therapeutic effect on patients with brain metastasis (BM). A BM preclinical model based on a multi-organ microfluidic chip has been established proficiently in our previous work. In this study, the BM subpopulation (PC9-Br) derived from ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:612091]

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Mechanical and Physical Regulation of Fibroblast-Myofibroblast Transition: From Cellular Mechanoresponse to Tissue Pathology.

Mirko D'Urso, Nicholas A Kurniawan,

Fibroblasts are cells present throughout the human body that are primarily responsible for the production and maintenance of the extracellular matrix (ECM) within the tissues. They have the capability to modify the mechanical properties of the ECM within the tissue and transition into myofibroblasts, a cell type that is associated ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:609653]

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Influence of Extracellular Vesicles Isolated From Osteoblasts of Patients With Cox-Arthrosis and/or Osteoporosis on Metabolism and Osteogenic Differentiation of BMSCs.

Tanja Niedermair, Christoph Lukas, Shushan Li, Sabine Stöckl, Benjamin Craiovan, Christoph Brochhausen, Marianne Federlin, Marietta Herrmann, Susanne Grässel,

<b>Background:</b> Studies with extracellular vesicles (EVs), including exosomes, isolated from mesenchymal stem cells (MSC) indicate benefits for the treatment of musculoskeletal pathologies as osteoarthritis (OA) and osteoporosis (OP). However, little is known about intercellular effects of EVs derived from pathologically altered cells that might influence the outcome by counteracting effects ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:615520]

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Transparent PDMS Bioreactors for the Fabrication and Analysis of Multi-Layer Pre-vascularized Hydrogels Under Continuous Perfusion.

Juan Liu, Huaiyuan Zheng, Xinyi Dai, Patrina S P Poh, Hans-Günther Machens, Arndt F Schilling,

Tissue engineering in combination with stem cell technology has the potential to revolutionize human healthcare. It aims at the generation of artificial tissues that can mimic the original with complex functions for medical applications. However, even the best current designs are limited in size, if the transport of nutrients and ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:568934]

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Improving Biological Treatment of Real Bilge Wastewater With Zero Valent Iron and Activated Charcoal Addition.

Aikaterini A Mazioti, Gregoris Notarides, Giannis Symeou, Ioannis Vyrides,

From the ships engine rooms a recalcitrant wastewater is produced called "bilge" which contains oil, metal working fluids, surfactants, and salinity. This study investigated the treatment of real bilge wastewater in short experiments using the following processes: (i) anaerobic digestion with granular sludge and ZVI addition for enhancement of methane ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:614510]

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Verification of Finger Joint Stiffness Estimation Method With Soft Robotic Actuator.

Xiang Qian Shi, Ho Lam Heung, Zhi Qiang Tang, Kai Yu Tong, Zheng Li,

Stroke has been the leading cause of disability due to the induced spasticity in the upper extremity. The constant flexion of spastic fingers following stroke has not been well described. Accurate measurements for joint stiffness help clinicians have a better access to the level of impairment after stroke. Previously, we ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:592637]

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Metal Organic Frame-Upconverting Nanoparticle Assemblies for the FRET Based Sensor Detection of Bisphenol A in High-Salt Foods.

Zhou Xu, Lin-Wei Zhang, Ling-Li Long, Shao-Hua Zhu, Mao-Long Chen, Li Ding, Yun-Hui Cheng,

To resolve the occurrence of unfulfillable detection in high-salts foods, we used fluorescence resonant energy transfer (FRET) sensors based on nanoparticle upconversion. In this study, we developed a novel FRET sensor for the detection of bisphenol A (BPA) in high-salt foods. We based this approach on the assembly of aptamer ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:626269]

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Does Blast Exposure to the Torso Cause a Blood Surge to the Brain?

Jose E Rubio, Maciej Skotak, Eren Alay, Aravind Sundaramurthy, Dhananjay Radhakrishnan Subramaniam, Vivek Bhaskar Kote, Stewart Yeoh, Kenneth Monson, Namas Chandra, Ginu Unnikrishnan, Jaques Reifman,

The interaction of explosion-induced blast waves with the torso is suspected to contribute to brain injury. In this indirect mechanism, the wave-torso interaction is assumed to generate a blood surge, which ultimately reaches and damages the brain. However, this hypothesis has not been comprehensively and systematically investigated, and the potential ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:573647]

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Streamlining Natural Products Biomanufacturing With Omics and Machine Learning Driven Microbial Engineering.

Ahmad Bazli Ramzi, Syarul Nataqain Baharum, Hamidun Bunawan, Nigel S Scrutton,

Increasing demands for the supply of biopharmaceuticals have propelled the advancement of metabolic engineering and synthetic biology strategies for biomanufacturing of bioactive natural products. Using metabolically engineered microbes as the bioproduction hosts, a variety of natural products including terpenes, flavonoids, alkaloids, and cannabinoids have been synthesized through the construction and ... Read more >>

Front Bioeng Biotechnol (Frontiers in bioengineering and biotechnology)
[2020, 8:608918]

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