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Journal Curr Protoc Mouse Biol

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Tackling the Mouse-on-Mouse Problem in Cochlear Immunofluorescence: A Simple Double-Blocking Protocol for Immunofluorescent Labeling of Murine Cochlear Sections with Primary Mouse Antibodies.

Mohamed Bassiouni, Katharina Stölzel, Alina Smorodchenko, Heidi Olze, Agnieszka J Szczepek,

The mouse is the most widely used animal model in hearing research. Immunohistochemistry and immunofluorescent staining of murine cochlear sections have, thus, remained a backbone of inner ear research. Since many primary antibodies are raised in mouse, the problem of "mouse-on-mouse" background arises due to the interaction between the anti-mouse ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(4):e84]

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An Ex Vivo Acid Injury and Repair (AIR) Model Using Precision-Cut Lung Slices to Understand Lung Injury and Repair.

Sally Yunsun Kim, Róisín Mongey, Mark Griffiths, Matthew Hind, Charlotte H Dean,

Recent advances in cell culture models like air-liquid interface culture and ex vivo models such as organoids have advanced studies of lung biology; however, gaps exist between these models and tools that represent the complexity of the three-dimensional environment of the lung. Precision-cut lung slices (PCLS) mimic the in vivo ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(4):e85]

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Phenotyping in Mice Using Continuous Home Cage Monitoring and Ultrasonic Vocalization Recordings.

Liane Hobson, Rasneer S Bains, Simon Greenaway, Sara Wells, Patrick M Nolan,

Over the last century, the study of mouse behavior has uncovered insights into brain molecular mechanisms while revealing potential causes of many neurological disorders. To this end, researchers have widely exploited the use of mutant strains, including those generated in mutagenesis screens and those produced using increasingly sophisticated genome engineering ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(3):e80]

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Assessment of Problem-Solving Skills and Inhibitory Control in Mice Using Water Escape Detour Test.

Grzegorz R Juszczak, Anna Bobrowska,

Despite the importance of emotional intelligence, its biological mechanism is still not well understood. For this reason, we have developed a rodent detour task which requires an animal to reach a highly desired object placed directly behind a transparent barrier that blocks the direct route to the target. This apparently ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(3):e82]

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Behavioral Phenotyping for Down Syndrome in Mice.

Randall J Roper, Charles R Goodlett, María Martínez de Lagrán, Mara Dierssen,

Down syndrome (DS) is the most frequent genetic cause of intellectual disability, characterized by alterations in different behavioral symptom domains: neurodevelopment, motor behavior, and cognition. As mouse models have the potential to generate data regarding the neurological basis for the specific behavioral profile of DS, and may indicate pharmacological treatments ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(3):e79]

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Quantitative Proteomic Profiling of Murine Ocular Tissue and the Extracellular Environment.

Jason Yeung, Jeffrey Lamb, Jonathan R Krieger, Mihaela Gadjeva, Jennifer Geddes-McAlister,

Mass spectrometry-based proteomics provides a robust and reliable method for detecting and quantifying changes in protein abundance among samples, including cells, tissues, organs, and supernatants. Physical damage or inflammation can compromise the ocular surface permitting colonization by bacterial pathogens, commonly Pseudomonas aeruginosa, and the formation of biofilms. The interplay between ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(3):e83]

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Simultaneous Assessment of Circadian Rhythms and Sleep in Mice Using Passive Infrared Sensors: A User's Guide.

Laurence A Brown, Gareth T Banks, Neil Horner, Sian L Wilcox, Patrick M Nolan, Stuart N Peirson,

The 24-hr cycle of activity and sleep provides perhaps the most familiar example of circadian rhythms. In mammals, circadian activity rhythms are generated by a master biological clock located in the hypothalamic suprachiasmatic nuclei (SCN). This clock is synchronized (entrained) to the external light environment via light input from retinal ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(3):e81]

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Fundamentals of Microscopy.

Jeremy Sanderson,

The light (or optical) microscope is the icon of science. The aphorism "seeing is believing" is often quoted in scientific papers involving microscopy. Unlike many scientific instruments, the light microscope will deliver an image however badly it is set up. Fluorescence microscopy is a widely used research tool across all ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(2):e76]

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Whole-Mount In Situ Hybridization in Post-Implantation Staged Mouse Embryos.

Kristen S Barratt, Ruth M Arkell,

Understanding RNA expression in space and time is a key initial step in dissecting gene function. The ability to visualize gene expression in whole-tissue or whole-specimen preparations, called in situ hybridization (ISH), was first developed 50 years ago. Two decades later, these protocols were adapted to establish robust methods for ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(2):e75]

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Production of Digoxigenin-Labeled Riboprobes for In Situ Hybridization Experiments.

Kristen S Barratt, Ruth M Arkell,

Experiments that visualize gene expression in intact tissues or organisms are fundamental to studies of gene function. These experiments, called in situ hybridization, require the production of a riboprobe, which is a labeled antisense RNA corresponding to a particular gene. The most commonly used system for visualizing gene expression via ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(2):e74]

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Whole Mount In Situ Hybridization of Mid-Gestation Mouse Embryos.

Annemiek Beverdam,

In situ hybridization is a powerful technique that allows the visualization of specific RNA species in biological samples in exquisite detail. It has been particularly well explored in the field of developmental genetics. The spatial and temporal patterns of RNA expression provide us with critical information on likely gene function ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(2):e77]

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Simple Protocol for Generating and Genotyping Genome-Edited Mice With CRISPR-Cas9 Reagents.

Almudena Fernández, Matías Morín, Diego Muñoz-Santos, Santiago Josa, Andrea Montero, Marcos Rubio-Fernández, Marta Cantero, Julia Fernández, María Jesús Del Hierro, Marta Castrillo, Miguel Ángel Moreno-Pelayo, Lluís Montoliu,

The simple protocol described in this article aims to provide all required information, as a comprehensive, easy-to-follow step-by-step method, to ensure the generation of the expected genome-edited mice. Here, we provide protocols for the preparation of CRISPR-Cas9 reagents for microinjection and electroporation into one-cell mouse embryos to create knockout or ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(1):e69]

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A Novel One-Day Learning Procedure for Mice.

Martin Darvas, Kishore Mukherjee, Amanda Lee, Warren Ladiges,

Current preclinical cognitive assessments are highly time intensive, with lengthy assessment procedures. In this regard, a single-day assay that focuses just on assessing learning behavior in a time-effective and relatable manner would be of value. This article describes the box maze as a short-term behavioral procedure to measure learning in ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(1):e68]

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Efficient Generation of Large-Fragment Knock-In Mouse Models Using 2-Cell (2C)-Homologous Recombination (HR)-CRISPR.

Bin Gu, Eszter Posfai, Marina Gertsenstein, Janet Rossant,

Generating large-fragment knock-ins, such as reporters, conditional alleles, or humanized alleles, directly in mouse embryos is still a challenging feat. We have developed 2C-HR-CRISPR, a technology that allows highly efficient (10-50%) and rapid (generating founders in 2 months) targeting of large DNA fragments. Key to this strategy is the delivery ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2020, 10(1):e67]

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Optimizing PCR for Mouse Genotyping: Recommendations for Reliable, Rapid, Cost Effective, Robust and Adaptable to High-Throughput Genotyping Protocol for Any Type of Mutation.

Sylvie Jacquot, Nathalie Chartoire, Françoise Piguet, Yann Hérault, Guillaume Pavlovic,

Genotyping consists of searching for a DNA sequence variation localized at a well-defined locus in the genome. It is an essential step in animal research because it allows the identification of animals that will be bred to generate and maintain a colony, euthanized to control the available space in the ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2019, 9(4):e65]

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Designing a QTL Mapping Study for Implementation in the Realized Collaborative Cross Genetic Reference Population.

Morris Soller, Hanifa J Abu-Toamih Atamni, Ilona Binenbaum, Aristotelis Chatziioannou, Fuad A Iraqi,

The Collaborative Cross (CC) mouse resource is a next-generation mouse genetic reference population (GRP) designed for high-resolution mapping of quantitative trait loci (QTL) of large effect affecting complex traits during health and disease. The CC resource consists of a set of 72 recombinant inbred lines (RILs) generated by reciprocal crossing ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2019, 9(4):e66]

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Genomic Mutation Identification in Mice Using Illumina Sequencing and Linux-Based Computational Methods.

John A Williams, George Powell, Ann-Marie Mallon, Michelle M Simon,

Genetically modified mice are an essential tool for modeling disease-causing mechanisms and discovering gene function. SNP genotyping was traditionally used to associate candidate regions with traits in the mouse, but failed to reveal novel variants without further targeted sequencing. Using a robust set of computational protocols, we present a platform ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2019, 9(3):e64]

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High Resolution Imaging of Mouse Embryos and Neonates with X-Ray Micro-Computed Tomography.

Chih-Wei Hsu, Sowmya Kalaga, Uchechukwu Akoma, Tara L Rasmussen, Audrey E Christiansen, Mary E Dickinson,

Iodine-contrast micro-computed tomography (microCT) 3D imaging provides a non-destructive and high-throughput platform for studying mouse embryo and neonate development. Here we provide protocols on preparing mouse embryos and neonates between embryonic day 8.5 (E8.5) to postnatal day 4 (P4) for iodine-contrast microCT imaging. With the implementation of the STABILITY method ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2019, 9(2):e63]

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Ultrasound-Guided Approaches to Improve Orthotopic Mouse Xenograft Models for Hepatocellular Carcinoma.

Ghina Bou About, Emilie Thiebault, Marie Wattenhofer-Donzé, Hugues Jacobs, Alain Guimond, Tania Sorg, Eric Robinet, Thomas F Baumert, Laurent Monassier, Yann Herault,

Hepatocellular carcinoma (HCC) is the second leading cause of cancer death worldwide. While curative approaches for early stage HCC exist, effective treatment options for advanced HCC are lacking. Furthermore, there are no efficient chemopreventive strategies to limit HCC development once cirrhosis is established. One challenge for drug development is unsatisfactory ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2019, 9(2):e62]

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Assessing Blood Clotting and Coagulation Factors in Mice.

Marisa A Brake, Lacramioara Ivanciu, Susan A Maroney, Nicolas D Martinez, Alan E Mast, Randal J Westrick,

The mammalian blood coagulation system was designed to restrict blood loss due to injury as well as keep the blood fluid within the blood vessels of the organism. Blood coagulation activity in inbred mouse strains varies widely among strains, suggesting that many genomic variants affect hemostasis. Some of these molecules ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2019, 9(2):e61]

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Housing Gnotobiotic Mice in Conventional Animal Facilities.

Máximo E Lange, Richard R E Uwiera, G Douglas Inglis,

Gnotobiotic mice are an established, robust model utilized in current research to study host-microbiota interactions. For years isolators have been used to rear germ-free and gnotobiotic mice. However, isolators can be costly and the segregation of treatments within the same isolator is problematic. Recently, methodologies for housing germ-free mice in ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2019, 9(1):e59]

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Viral Vectors for Gene Transfer.

Yong Hong Chen, Megan S Keiser, Beverly L Davidson,

Viral vectors are a promising tool for effective delivery of genetic material into cells. They take advantage of the natural ability of a virus to deliver a genetic payload into cells while being genetically modified such that their ability to replicate is crippled or removed. Here, an updated overview of ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2018, 8(4):e58]

Cited: 6 times

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Techniques for Intracranial Stereotaxic Injections of Adeno-Associated Viral Vectors in Adult Mice.

Megan S Keiser, Yong Hong Chen, Beverly L Davidson,

Stereotaxic intracranial injection of viral vectors is a valuable technique to directly deliver genetic material to a specific population of cells in the central nervous system of a mouse model. This enables scientists to test candidate gene therapies or disease modulators that can then provide insight into the pathological mechanisms ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2018, 8(4):e57]

Cited: 1 time

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Studying Osteoarthritis Pathogenesis in Mice.

Andrew Blease, Patricia Das Neves Borges, Marcia Curtinha, Behzad Javaheri, Isabell S von Loga, Ida Parisi, Jadwiga Zarebska, Andrew Pitsillides, Tonia L Vincent, Paul K Potter,

With the increasing availability and complexity of mouse models of disease, either spontaneous or induced, there is a concomitant increase in their use in the analysis of pathogenesis. Among such diseases is osteoarthritis, a debilitating disease with few treatment options. While advances in our understanding of the pathogenesis of osteoarthritis ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2018, 8(4):e50]

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Muscle Stem Cell Immunostaining.

Shuaiyu Wang, Bao Zhang, Gregory C Addicks, Hui Zhang, Keir J Menzies, Hongbo Zhang,

Muscle stem cells (MuSCs) are essential for maintaining muscle homeostasis by providing progenitor cells for muscle regeneration after injury and in muscular diseases. MuSC properties dynamically change, reflecting physiology or pathological status. For instance, MuSCs are activated after muscle injury, but become exhausted in late stages of Duchenne Muscular Dystrophy ... Read more >>

Curr Protoc Mouse Biol (Current protocols in mouse biology)
[2018, 8(3):e47]

Cited: 1 time

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