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Purification and characterization of glucoamylase of Aspergillus oryzae from Luzhou-flavour Daqu.

Chuan Wang, Lianli Yang, Lunan Luo, Shichao Tang, Qiang Wang,

OBJECTIVE:To obtain novel glucoamylase from Daqu microbe. RESULTS:A dominant strain known as LZ2 with high activity of hydrolyzing starch was isolated from Luzhou Daqu, a Chinese traditional fermentation starter. The LZ2 was identified as Aspergillus oryzae by 18S rDNA sequence analysis. Glucoamylase from LZ2, named as GA-LZ2, was purified to ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Inhibition of nattokinase against the production of poly (γ-glutamic Acid) in Bacillus subtilis natto.

Li Wang, Ning Liu, Chenrui Yu, Junliu Chen, Kangjin Hong, Yipeng Zang, Mengmeng Wang, Guangjun Nie,

OBJECTIVE:To study the effect of nattokinse (NK) on the synthesis of poly(γ-glutamic acid) (γ-PGA) in Bacillus subtilis natto. RESULTS:γ-PGA yield significantly decreased as NK was added in the original medium. With the increment of NK dosage, the yield decreased increasingly, but biomass increased instead of decreasing. The fact that cell ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Improving enzyme activity of glucosamine-6-phosphate synthase by semi-rational design strategy and computer analysis.

Piwu Li, Kang Li, Xu Li, Fei Zhao, Ruiming Wang, Junqing Wang,

OBJECTIVE:To improve enzyme activity of Glucosamine-6-phosphate synthase (Glms) of Bacillus subtilis by site saturation mutagenesis at Leu593, Ala594, Lys595, Ser596 and Val597 based on computer-aided semi-rational design. RESULTS:The results indicated that L593S had the greatest effect on the activity of BsGlms and the enzyme activity increased from 5 to 48 ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Co-evolution of β-glucosidase activity and product tolerance for increasing cellulosic ethanol yield.

Kexin Wang, Qiuxia Huang, Hanxin Li, Xihua Zhao,

β-Glucosidase (BGL) plays a key role in cellulose hydrolysis. However, it is still a great challenge to enhance product tolerance and enzyme activity of BGL simultaneously. Here, we utilized one round error-prone PCR to engineer the Penicillium oxalicum 16 BGL (16BGL) for improving the cellulosic ethanol yield. We identified a ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Investigation of the gut microbiome of Apis cerana honeybees from Vietnam.

Bui Thi Thuy Duong, Nguyen Thi Kim Lien, Ha Thi Thu, Nguyen Thi Hoa, Pham Thi Lanh, Bo-Ram Yun, Mi-Sun Yoo, Yun Sang Cho, Dong Van Quyen,

OBJECTIVES:In this study, the gut microbiome of healthy adult honeybees, Apis cerana, was investigated by sequencing the V3 - V4 region in 16S rRNA gene using Illumina Miseq platform. RESULTS:The total of 37,853 reads for 16S rRNA gene were obtained and 30,121 (79.6%) reads were valid with 25,291 (84.0%) reads ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Enhancement of S-adenosylmethionine production by deleting thrB gene and overexpressing SAM2 gene in Bacillus amyloliquefaciens.

Cong Jiang, Liying Ruan, Xuetuan Wei, Ailing Guo,

OBJECTIVES:To improve the S-adenosylmethionine (SAM) production in methionine-free medium, effects of deleting genes of SAM decarboxylase (speD) and homoserine kinase (thrB) on SAM titers were investigated, and the SAM synthetase gene (SAM2) was also overexpressed. RESULTS:In B. amyloliquefaciens HSAM2, deleting speD to block the SAM utilization pathway significantly reduced the ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Recombinant ferritin nanoparticles can induce dendritic cell maturation through TLR4/NF-κB pathway.

Zhehui Qu, Yongli Guo, Mingzhu Li, Chong Cao, Junwei Wang, Mingchun Gao,

OBJECTIVE:Immune response initiation and regulation require activation of dendritic cells (DCs). However, the mechanism by which ferritin, a carrier for immunogen, induces DCs maturation remains unclear. RESULTS:Recombinant ferritin nanoparticle (RFNp), were prepared through the baculovirus expression vector system, formed spherical and hollow cage-liked proteins with a diameter of approximately 12.17 ± 0.87 nm. ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Aerobic biodegradation of high explosive hexahydro-1,3,5- trinitro-1,3,5-triazine by Janibacter cremeus isolated from contaminated soil.

Anchita Kalsi, S Mary Celin, Jai Gopal Sharma,

OBJECTIVE:To evaluate the ability of Janibacter cremeus a soil bacterium isolated from explosive contaminated site in degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and to study enzyme responsible for degradation. RESULTS:The isolate exhibited 88% degradation of RDX in 30 days of incubation. The biodegradation process followed the first order kinetics. The half- life of ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Correction to: Bioconversion of arachidonic acid into human 14,15-hepoxilin B3 and 13,14,15-trioxilin B3 by recombinant cells expressing microbial 15-lipoxygenase without and with epoxide hydrolase.

J Lee, T-H Kim, D-K Oh,

In the original publication of the article, some chirality of Fig. 1 was published incorrectly. The corrected figure is provided below. ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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By targeting TRAF6, miR-140-3p inhibits TGF-β1-induced human osteosarcoma epithelial-to-mesenchymal transition, migration, and invasion.

Qianchen Guo, Nai Zhang, Shen Liu, Zixuan Pang, Zhao Chen,

OBJECTIVES:We evaluated the effects of miR-140-3p on EMT, cellular migration, and invasion in TGF-β1 treated human OS cells. Human fresh OS tissue and normal bone tissue specimens were gathered from 42 patients (29 male and 13 female, 11 to 24 years of age with a mean age of 17.5 ± 2.3 years) diagnosed with ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Engineered formate dehydrogenase from Chaetomium thermophilum, a promising enzymatic solution for biotechnical CO2 fixation.

Mehmet M Çakar, Jouni Ruupunen, Juan Mangas-Sanchez, William R Birmingham, Deniz Yildirim, Ossi Turunen, Nicholas J Turner, Jarkko Valjakka, Barış Binay,

OBJECTIVES:Formate dehydrogenases (FDHs) are NAD(P)H-dependent enzymes that catalyse the reversible oxidation of formate to CO2. The main goal was to use directed evolution to obtain variants of the FDH from Chaetomium thermophilum (CtFDH) with enhanced reduction activity in the conversion of CO2 into formic acid. RESULTS:Four libraries were constructed targeting ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Inhibition of berberine hydrochloride on Candida albicans biofilm formation.

Xiaoxue Huang, Mingyue Zheng, Yuling Yi, Anamica Patel, Zhen Song, Yan Li,

OBJECTIVE:This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS:This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 μg/mL BH significantly inhibited ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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A novel system to rapidly detect protein-protein interactions (PPIs) based on fluorescence co-localization.

Nan Hu, Zhan-Qi Dong, Ting-Ting Chen, Ning Zheng, Qin Wu, Peng Chen, Cheng Lu, Min-Hui Pan,

OBJECTIVE:Rapid and convenient detection of protein-protein interactions (PPIs) is of great significance for understanding function of protein. RESULTS:For efficiently detecting PPIs, we used the changes of proteins fluorescence localization to design a novel system, fluorescence translocation co-localization (FTCL), based on nuclear localization signal (NLS) in living cells. Depending on the ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Purification and characterization of a native lytic polysaccharide monooxygenase from Thermoascus aurantiacus.

Susanne Fritsche, Cynthia Hopson, Jennifer Gorman, Raphael Gabriel, Steven W Singer,

Lytic polysaccharide monooxygenases (LPMOs) have emerged as key proteins for depolymerization of cellulose. These copper-containing enzymes oxidize C-1 and/or C-4 bonds in cellulose, promoting increased hydrolysis of the oxidized cellulose chains. The LPMO from Thermoascus aurantiacus, a thermophilic ascomycete fungus, has been extensively studied and has served as a model ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Overexpression and characterization of a novel GH16 β-agarase (Aga1) from Cellulophaga omnivescoria W5C.

Kristine Rose M Ramos, Kris Niño G Valdehuesa, Angelo B Bañares, Grace M Nisola, Won-Keun Lee, Wook-Jin Chung,

OBJECTIVE:To identify and characterize a new β-agarase from Cellulophaga omnivescoria W5C capable of producing biologically-active neoagarooligosaccharides from agar. RESULTS:The β-agarase, Aga1, has signal peptides on both N- and C-terminals, which are involved in the type IX secretion system. It shares 75% protein sequence identity with AgaD from Zobellia galactanivorans and ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Neuroprotection of melatonin on spinal cord injury by activating autophagy and inhibiting apoptosis via SIRT1/AMPK signaling pathway.

Kai Gao, Jianbing Niu, Xiaoqian Dang,

The effect of melatonin (MT) on spinal cord injury (SCI) has attracted increasing research attention. However, the specific role and molecular mechanism of MT on SCI have not been elucidated. An experiment was performed to investigate the effect and molecular mechanism of MT on the neuronal autophagy after SCI and ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Production and characterization of an enzyme extract with cellulase activity produced by an indigenous strain of Fusarium verticillioides ITV03 using sweet sorghum bagasse.

María Inés Infanzón-Rodríguez, Juan Arturo Ragazzo-Sánchez, Sandra Del Moral, Montserrat Calderón-Santoyo, María Guadalupe Aguilar-Uscanga,

OBJECTIVES:To evaluate a strain of Fusarium verticillioides ITV03 isolated from wood residues in the Veracruz region of Mexico. Endoglucanase and β-glucosidase production by submerged fermentation was optimized using a Box-Behnken design, where the independent variables were urea, ammonium sulfate and yeast extract. RESULTS:After optimization, an endoglucanase activity of 0.27 U/mL was ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Improvement of the recombinant human coagulation factor IX expression by co-expression of a novel transcript of Drosophila γ carboxylase in a human cell line.

Solmaz Moniri Javadhesari, Alireza Zomorodipour,

OBJECTIVE:Mammalian cells as the main host for production of human proteins are incapable of complete γ-carboxylation of over-expressed Vitamin K Dependent (VKD) proteins. The Drosophila γ-glutamyl carboxylase (DγC) has been shown to be more efficient than its human counterpart in γ-carboxylation of human substrates, in vitro. Considering the Drosophila γ-carboxylase ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method.

Yan-Yan Wei, Qun-Mei Zhan, Xiang-Xing Zhu, Ai-Fen Yan, Juan Feng, Lian Liu, Jian-Hao Li, Dong-Sheng Tang,

OBJECTIVES:Guangdong Small-ear Spotted (GDSS) pigs are a pig breed native to China that possesses unfortunate disadvantages, such as slow growth rate, low lean-meat percentage, and reduced feed utilization. In contrast to traditional genetic breeding methods with long cycle time and high cost, CRISPR/Cas9-mediated gene editing for the modification of the ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Piperine inhibits colorectal cancer migration and invasion by regulating STAT3/Snail-mediated epithelial-mesenchymal transition.

Lingyu Song, Yan Wang, Yunhuan Zhen, Dengke Li, Xidong He, Hong Yang, Huayang Zhang, Qi Liu,

Cancer metastasis is the primary cause of death in patients diagnosed with colorectal cancer. Piperine, an active nontoxic ingredient in pepper, has potent anti-inflammatory and anti-cancer properties. However, little is known about the anti-migratory and anti-invasive effects of piperine on colorectal cancer. We demonstrated piperine inhibited the migration and invasion ... Read more >>

Biotechnol. Lett. (Biotechnology letters.)
[2020, :]

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Nanoparticle technology improves in-vitro attachment of cattle (Bos taurus) trophectoderm cells.

Jaewook Chung, Ganesh Sriram, Carol L Keefer,

The bovine cell line, cow trophectoderm-1 (CT-1), provides an excellent in-vitro cell culture model to study early embryonic development. Obtaining consistent attachment and outgrowth, however, is difficult because enzymatic disassociation into single cells is detrimental; therefore, CT-1 cells must be passaged in clumps, which do not attach readily to the ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Improving the thermostability of a GH97 dextran glucosidase by rational design.

Xiaomin Zhang, Feiyun Chen, Chao He, Wei Fang, Zemin Fang, Xuecheng Zhang, Xiaotang Wang, Yazhong Xiao,

This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using the TKSA-MC program based on the optimization of surface charge-charge interactions and were mutated to glutamate for ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Codon usage trend in genes associated with obesity.

Supriyo Chakraborty, Parvin A Barbhuiya, Sunanda Paul, Arif Uddin, Yashmin Choudhury, Yeongseon Ahn, Yoon Shin Cho,

Obesity is not only a social menace but also an economic burden as it reduces productivity and increases health care cost. We used bioinformatic tools to analyze the CUB of obesity associated genes and compared with housekeeping genes (control) to explore the similarities and differences between two data sets as ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines.

Rafael A Donassolo, Marcos Roberto A Ferreira, Clóvis Moreira, Lucas M Dos Santos, Emili Griep, Gustavo M S G Moreira, Rafael R Rodrigues, Ângela N Moreira, Fabricio R Conceição,

OBJECTIVES:Earlier studies have demonstrated the use of inactivated recombinant E. coli (bacterins), to protect against Clostridium spp. in vaccinated animals. These bacterins have a simpler, safer, and faster production process. However, these bacterins carry expression plasmids, containing antibiotic resistance gene, which could be assimilate accidentally by environmental microorganisms. Considering this, ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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Continuous biomass and lipid production from local chlorella-bacteria consortium in raw wastewater using volatile fatty acids.

Yongmei Zuo, Tassnapa Wongsnansilp, Xiaodong Zhang, Guiping Chen, Zhe Wu,

OBJECTIVES:To evaluate mixotrophic cultivation of microalgae-bacteria consortium in raw wastewater by stepwise addition of fermented effluent containing volatile fatty acids (VFAs). RESULTS:Stepwise increase of VFAs enhanced algal biomass and lipid production, ammonia and phosphate removals. The highest biomass and lipid yield were 1.94 g L-1 and 310 mg L-1 when the addition ... Read more >>

Biotechnol. Lett. (Biotechnology letters)
[2020, :]

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