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Endogenous localization of TOP-2 in C. elegans using a C-terminal GFP-tag.

Christine K Rourke, Darline Murat, Tyler J Hansen, Aimee Jaramillo-Lambert,

To investigate the dynamic localization of Topoisomerase II in live <i>C. elegans</i> we have generated a C-terminally GFP-tagged version of TOP-2 at the endogenous locus. We found that TOP-2::GFP localizes in a similar pattern to the previously published TOP-2::3XFLAG strain and does not disrupt the meiotic chromosome segregation functions of ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Mutating two putative phosphorylation sites on ZHP-3 does not affect its localization or function during meiotic chromosome segregation.

Anna E Russo, Christian R Nelson, Needhi Bhalla,

Meiotic chromosome segregation depends on crossover recombination to link homologous chromosomes together and promote accurate segregation in the first meiotic division. In <i>Caenorhabditis elegans</i>, a conserved RING finger protein, ZHP-3, is essential for meiotic recombination and localizes to sites of crossover formation. Whether ZHP-3 is regulated to promote recombination remains ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Mutations in the Physcomitrium patens gene encoding Aminodeoxychorismate Synthase confer auxotrophic phenotypes.

Michael J Prigge, Yingluo Wang, Mark Estelle,

To facilitate genetic mapping of developmental mutants of <i>Physcomitrium patens</i>, we produced a genetic marker that combines recessive auxotrophy with dominant positive selection. We first identified the gene affected by the <i>pabB4</i> auxotrophic mutation and then replaced it with a cassette that confers antibiotic resistance. This strain may be used ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Comparison of N- and C-terminally endogenously GFP-tagged WEE-1.3 strains in C. elegans.

Lourds M Fernando, Kyrionna Golliday, Ruby Boateng, Anna K Allen,

We have generated a WEE-1.3 strain in <i>C. elegans</i> wherewe have endogenously tagged the C-terminus with GFP<i>.</i> In this publication we demonstrate that this new strain exhibits the same expression localization pattern as the WEE-1.3 antibody and N-terminally endogenously GFP-tagged WEE-1.3 strain that have been previously published. We also show ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

Cited: 1 time

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Engineering essential genes with a "jump board" strategy using CRISPR/Cas9.

Ye Duan, Sungwook Choi, Charles Nelson, Victor Ambros,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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fs(1)A13041 is a 5' UTR deletion of the essential gene small ovary in Drosophila.

Myles Hammond, Jillian G Gomez, Brian Oliver, Steve Kucera, Leif Benner,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca.

Erika B Sorensen, Hannah S Seidel, Sarah L Crittenden, Joseph H Ballard, Judith Kimble,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

Cited: 1 time

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Difference in phenotypic severity of presumed null alleles of capg-1.

Sarah VanDiepenbos, Györgyi Csankovszki,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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-Using the C. elegans lem-2 Gene to Reconstruct the Human LEMD2 Mutation Associated with Hutterite-type Cataract/Cardiomyopathy.

Ayaa AlKhaleefa, Frances L Snider, Henry J Duff, James D McGhee,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The mir-35-42 binding site in the nhl-2 3'UTR is dispensable for development and fecundity.

Bing Yang, Katherine McJunkin,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

Cited: 1 time

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Auxin-independent depletion of degron-tagged proteins by TIR1.

Erin Schiksnis, Angela Nicholson, Matthew Modena, Makena Pule, Joshua Arribere, Amy Pasquinelli,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

Cited: 2 times

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The autophagy gene product BEC-1 supports normal aging and neurodevelopment in Caenorhabditis elegans I.

Nicholas Ashley, Andrea Holgado,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

Cited: 2 times

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The autophagy gene product BEC-1 supports normal aging and neurodevelopment in Caenorhabditis elegans: Integration.

Nicholas Ashley, Andrea Holgado,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

Cited: 0 times

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A CRISPR/Cas9-generated cdc-7 loss of function mutation does not cause temperature-dependent fertility defects.

Heather Currey, Nicole Liachko,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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Disruption of the mutator complex triggers a low penetrance larval arrest phenotype.

Alicia Kathryn Rogers, Carolyn Marie Phillips,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Attenuation of porcine deltacoronavirus disease severity by porcine reproductive and respiratory syndrome virus coinfection in a weaning pig model.

Xinrong Zhou, Xinna Ge, Yongning Zhang, Jun Han, Xin Guo, Yanhong Chen, Lei Zhou, Hanchun Yang,

Porcine deltacoronavirus (PDCoV) is a potentially emerging zoonotic pathogen that causes severe diarrhea in young pigs, with a risk of fatal dehydration. Its pathogenicity on neonatal piglet has been previously reported, however, it is less known if the coinfection with immunosuppressive pathogens can influence PDCoV disease manifestation. Here, a coinfection ... Read more >>

Virulence (Virulence)
[2021, 12(1):1011-1021]

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May mepolizumab used in asthma correct subfertility?

Guzin Ozden, Pelin Pınar Deniz,

<h4>İntroduction</h4>Asthma is one of the most common chronic airway disease among reproductive period of women. Chronic inflammation in asthma, eosinophilia, high steroid treatment and uncontrolled asthma may cause infertility by affecting the reproductive organs, menstrual cycle and quality of life. Some patients with severe asthma have frequent exacerbations associated with ... Read more >>

Ann Med (Annals of medicine)
[2021, 53(1):456-458]

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Effects of silencing epididymal vascular endothelial growth factor (VEGF) expression on hyaluronidase (HYD) activity in arsenic poisoning rats through downregulating VEGF receptor 2 (VEGFR2).

Yan-Ping Dai, Xiao-Qin Gao,

RNA interference (RNAi) was used to investigate the role of epididymal vascular endothelial growth factor (VEGF) gene expression on sperm hyaluronidase (HYD) in a rat model of arsenic poisoning and to identify a new gene therapy target for male infertility caused by arsenic poisoning. The Rat model of chronic arsenic ... Read more >>

Bioengineered (Bioengineered)
[2021, 12(1):1351-1359]

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Well-being in trainee and faculty physicians.

Gary N Frishman, Christina A Raker, David Frankfurter,

<b>Background:</b> Physician well-being remains a critical topic with limited information concerning the impact of the progression of training and duty hours. To date, our knowledge and interventions have not adequately addressed these issues. We assessed differences in well-being across the USA: (1) between all post-graduate trainees and their academic core ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1950107]

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Moral frameworks of commercial surrogacy within the US, India and Russia.

Marcin Smietana, Sharmila Rudrappa, Christina Weis,

In this paper, we draw on three ethnographic studies of surrogacy we carried out separately in different contexts: the western US state of California, the south Indian state of Karnataka, and the western Russian metropolis of St Petersburg. In our interviews with surrogate mothers, intended parents, and surrogacy professionals, we ... Read more >>

Sex Reprod Health Matters (Sexual and reproductive health matters)
[2021, 29(1):1-17]

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Validation of magnetic resonance relaxometry R2 value and cyst fluid iron level for diagnosis of ovarian endometrioma.

Shogo Imanaka, Yuki Yamada, Naoki Kawahara, Hiroshi Kobayashi,

<h4>Objectives</h4>Magnetic resonance (MR) R2 relaxometry is a safe, noninvasive diagnostic modality for the evaluation of iron levels in the contents of ovarian cysts. The study aims to investigate the sensitivity and specificity of the two methods, R2 value and iron level, in diagnosing OMA patients in the validation set.<h4>Methods</h4>A prospective ... Read more >>

Redox Rep (Redox report : communications in free radical research)
[2021, 26(1):105-110]

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Muslim men's perceptions and attitudes on family planning: a qualitative study in Wajir and Lamu counties in Kenya.

Batula Abdi, Jerry Okal, Gamal Serour, Marleen Temmerman,

In patriarchal societies like Kenya, understanding men's perceptions and attitudes on family planning is critical given their decision-making roles that affect uptake of contraception. Yet, most programmes mainly target women as primary users of contraceptive methods since they bear the burden of pregnancy. However, women-focused approaches tend to overlook gender ... Read more >>

Sex Reprod Health Matters (Sexual and reproductive health matters)
[2021, 29(1):1893890]

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gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication.

Yu Chen, Shanshan Zhu, Jiao Hu, Zenglei Hu, Xiaowen Liu, Xiaoquan Wang, Min Gu, Shunlin Hu, Xiufan Liu,

As the causative agent of Newcastle disease (ND), Newcastle disease virus (NDV) has seriously restricted the development of the poultry industry. Previous research has shown that miRNAs, members of the small noncoding RNA family, are implicated in the regulation NDV replication through extensive interactions with host mRNAs, but whether miRNAs ... Read more >>

Virulence (Virulence)
[2021, 12(1):45-56]

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Verbascoside attenuates experimental varicocele-induced damage to testes and sperm levels through up-regulation of the hypothalamus-pituitary-gonadal (HPG) axis.

Letian Han, Shan Xiang, Baohai Rong, Yanchen Liang, Shengtian Zhao,

<h4>Context</h4>Verbascoside (VB), which is found in many medicinal plant families, exhibits biological activities in various diseases. However, its effects on varicocele (VCL)-induced damage remain unknown.<h4>Objective</h4>To investigate the effects and mechanism of VB on experimental rats with varicocele (VCL)-induced damage.<h4>Materials and methods</h4>Sixty sexually mature male Sprague-Dawley (SD) rats were divided into ... Read more >>

Pharm Biol (Pharmaceutical biology)
[2021, 59(1):715-722]

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Seeking synergies: understanding the evidence that links menstrual health and sexual and reproductive health and rights.

Lucy C Wilson, Kate H Rademacher, Julia Rosenbaum, Rebecca L Callahan, Geeta Nanda, Sarah Fry, Amelia C L Mackenzie,

Global efforts to improve menstrual health and sexual and reproductive health and rights (SRHR) are fundamentally intertwined and share similar goals for improving health and well-being and increasing gender equality. Historically, however, the two fields have operated independently and missed opportunities to build upon their biological and sociocultural linkages. Biological ... Read more >>

Sex Reprod Health Matters (Sexual and reproductive health matters)
[2021, 29(1):1882791]

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