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Unspecific DNA recombination in AdipoqCre-ERT2 - mediated knockout approaches in transgenic mice is sex-, age- and genotype-dependent.

Andreas Lindhorst, Ingo Bechmann, Martin Gericke,

Due to the epidemic rise of obesity prevalence, adipose tissue (AT) research is of major interest. Our aim was to study specificity of the most-common Cre/loxP approach for inducible gene manipulation of AT in mice (AdipoqCre-ERT2). We used mice with tamoxifen-sensitive Cre recombinase controlled by the adiponectin promoter (AdipoqCre-ERT2), which ... Read more >>

Adipocyte (Adipocyte)
[2020, 9(1):1-6]

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Protective effects of ascorbic acid and calcitriol combination on airway remodelling in ovalbumin-induced chronic asthma.

Farzaneh Kianian, Seyed Morteza Karimian, Mehri Kadkhodaee, Nasrin Takzaree, Behjat Seifi, Hamid Reza Sadeghipour,

Context: Airway remodelling is one of the most refractory problems in asthma. According to the critical roles of oxidative stress and inflammation in airway remodelling, it is supposed that ascorbic acid and calcitriol have beneficial effects. However, a combination of antioxidants may be more effective for asthma therapy.Objective: This study ... Read more >>

Pharm Biol (Pharmaceutical biology)
[2020, 58(1):107-115]

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A qualitative examination of barriers against effective medical education and practices related to breastfeeding promotion and support in Lebanon.

Sara Moukarzel, Christoforos Mamas, Antoine Farhat, Antoine Abi Abboud, Alan J Daly,

Background: Insufficient breastfeeding promotion and support by physicians contribute to suboptimal breastfeeding rates globally. Understanding setting-specific barriers against breastfeeding promotion and support from the perspective of medical students and addressing those that can be modified through undergraduate medical education may help improve learning outcomes, medical practice, and ultimately health outcomes ... Read more >>

Med Educ Online (Medical education online)
[2020, 25(1):1723950]

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Percutaneous Repair of Ventricular Ruptures.

Rong Bing, Miles W.H. Behan, Renzo Pessotto, Nicholas L.M. Cruden, David B. Northridge,

Acquired ventricular wall ruptures can be life-threatening. Depending on the pathological features and anatomy, surgical repair can be technically… ... Read more >>

JACC Case Rep (Jacc. Case Reports)
[0, 2(3):341-346]

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Artificial Intelligence-Based Diagnosis of Cardiac and Related Diseases.

Muhammad Arsalan, Muhammad Owais, Tahir Mahmood, Jiho Choi, Kang Ryoung Park,

Automatic chest anatomy segmentation plays a key role in computer-aided disease diagnosis, such as for cardiomegaly, pleural effusion, emphysema, and pneumothorax. Among these diseases, cardiomegaly is considered a perilous disease, involving a high risk of sudden cardiac death. It can be diagnosed early by an expert medical practitioner using a ... Read more >>

J Clin Med (Journal of clinical medicine)
[2020, 9(3):]

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Invasive cardiovascular magnetic resonance (iCMR) for diagnostic right and left heart catheterization using an MR-conditional guidewire and passive visualization in congenital heart disease.

Surendranath R Veeram Reddy, Yousef Arar, Riad Abou Zahr, Vasu Gooty, Jennifer Hernandez, Amanda Potersnak, Phillip Douglas, Zachary Blair, Joshua S Greer, Sébastien Roujol, Mari Nieves Velasco Forte, Gerald Greil, Alan W Nugent, Tarique Hussain,

Today's standard of care, in the congenital heart disease (CHD) population, involves performing cardiac catheterization under x-ray fluoroscopy and cardiac magnetic resonance (CMR) imaging separately. The unique ability of CMR to provide real-time functional imaging in multiple views without ionizing radiation exposure has the potential to be a powerful tool for ... Read more >>

J Cardiovasc Magn Reson (Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance)
[2020, 22(1):20]

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A cardiomyocyte show of force: A fluorescent alpha-actinin reporter line sheds light on human cardiomyocyte contractility versus substrate stiffness.

Marcelo C Ribeiro, Rolf H Slaats, Verena Schwach, José M Rivera-Arbelaez, Leon G J Tertoolen, Berend J van Meer, Robert Molenaar, Christine L Mummery, Mireille M A E Claessens, Robert Passier,

Cardiovascular disease is often associated with cardiac remodeling, including cardiac fibrosis, which may lead to increased stiffness of the heart wall. This stiffness in turn may cause subsequent failure of cardiac myocytes, however the response of these cells to increased substrate stiffness is largely unknown. To investigate the contractile response ... Read more >>

J. Mol. Cell. Cardiol. (Journal of molecular and cellular cardiology)
[2020, :]

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RIP3/MLKL-mediated neuronal necroptosis induced by methamphetamine at 39°C.

Li-Min Guo, Zhen Wang, Shi-Ping Li, Mi Wang, Wei-Tao Yan, Feng-Xia Liu, Chu-Dong Wang, Xu-Dong Zhang, Dan Chen, Jie Yan, Kun Xiong,

Methamphetamine is one of the most prevalent drugs abused in the world. Methamphetamine abusers usually present with hyperpyrexia (39°C), hallucination and other psychiatric symptoms. However, the detailed mechanism underlying its neurotoxic action remains elusive. This study investigated the effects of methamphetamine + 39°C on primary cortical neurons from the cortex ... Read more >>

Neural Regen Res (Neural regeneration research)
[2020, 15(5):865-874]

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Comment on: Hybrid off-pump coronary artery bypass grafting surgery and transaortic transcatheter aortic valve replacement: Literature review of a feasible bailout for patients with complex coronary anatomy and poor femoral access.

Panagiotis Artemiou, Michal Hulman,

J Card Surg (Journal of cardiac surgery)
[2020, :]

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A patient with recurrent palpitations and unusual anatomy.

C J M Lawson, A D Margulescu, J Barry,

Neth Heart J (Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation)
[2020, :]

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A patient with recurrent palpitations and unusual anatomy.

C J M Lawson, A D Margulescu, J Barry,

Neth Heart J (Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation)
[2020, :]

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Cardiac anatomy pertinent to the catheter and surgical treatment of atrial fibrillation.

James L Cox, Andrei Churyla, Olga N Kislitsina, S Chris Malaisrie, Patrick M McCarthy,

In the 1980s when surgery was first introduced for the treatment of atrial fibrillation (AF), one would often hear comments like "Every little old grandmother has AF. Why on earth would you operate on someone like that?". ... Read more >>

J. Cardiovasc. Electrophysiol. (Journal of cardiovascular electrophysiology)
[2020, :]

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Creatine kinase rate constant in the human heart at 7T with 1D-ISIS/2D CSI localization.

Adil Bashir, Jianyi Zhang, Thomas S Denney,

PURPOSE:Creatine Kinase (CK) reaction plays an important role in energy metabolism and estimate of its reaction rate constant in heart provides important insight into cardiac energetics. Fast saturation transfer method ([Formula: see text] nominal) to measure CK reaction rate constant (kf) was previously demonstrated in open chest swine hearts. The ... Read more >>

PLoS ONE (PloS one)
[2020, 15(3):e0229933]

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Pulmonary vessels and bronchial anatomy of the left lower lobe.

Ryunosuke Maki, Masahiro Miyajima, Keishi Ogura, Makoto Tada, Yuki Takahashi, Wataru Arai, Hirofumi Adachi, Atsushi Watanabe,

PURPOSE:To identify and clarify the comprehensive anatomic patterns in the left lower lobe (LLL). METHODS:Using computed tomography (CT) imaging data, including that obtained using three-dimensional CT, we reviewed the anatomic patterns of the pulmonary vessels and bronchi in the left lungs of 539 patients, focusing on the LLL. RESULTS:The two-stem ... Read more >>

Surg. Today (Surgery today)
[2020, :]

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Applicability of the Zenith Inner Branched Arch Endograft.

Christopher R Burke, Jarin A Kratzberg, Anthony D Yoder, Natasha Z Steele, Gabriel S Aldea, Matthew P Sweet,

Purpose: To evaluate the clinical and anatomical features of patients with arch pathology to better understand the applicability of the Zenith inner branched arch endograft (IBAE). Materials and Methods: A retrospective review was performed of 60 consecutive patients (mean age 62.5 years; 42 men) who presented with nonruptured aortic arch ... Read more >>

J. Endovasc. Ther. (Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists)
[2020, 27(2):252-257]

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Current era outcomes of pulmonary atresia with ventricular septal defect: A single center cohort in Thailand.

Kanthalas Lertsakulpiriya, Chodchanok Vijarnsorn, Prakul Chanthong, Paweena Chungsomprasong, Supaluck Kanjanauthai, Kritvikrom Durongpisitkul, Jarupim Soongswang, Thaworn Subtaweesin, Somchai Sriyoschati,

Pulmonary atresia with ventricular septal defect (PA/VSD) is a complex cyanotic congenital heart disease with a wide-range of presentations and treatment strategies, depending on the source of pulmonary circulation, anatomy of pulmonary arteries (PAs), and major aortopulmonary collateral arteries (MAPCAs). Data about the outcomes in developing countries is scarce. We ... Read more >>

Sci Rep (Scientific Reports)
[2020, 10(1):5165]

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Cavotricuspid isthmus ablation for atrial flutter: Anatomic challenges and troubleshooting.

Georgios Christopoulos, Konstantinos C Siontis, Ugur Kucuk, Samuel J Asirvatham,

HeartRhythm Case Rep (HeartRhythm case reports)
[2020, 6(3):115-120]

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Guidelines for Animal Exercise and Training Protocols for Cardiovascular Studies.

David C Poole, Steven W Copp, Trenton D Colburn, Jesse C Craig, David Allen, Michael Sturek, Donal S O'Leary, Irving H Zucker, Timothy I Musch,

Whole body exercise tolerance is the consummate example of integrative physiological function among the metabolic, neuromuscular, cardiovascular and respiratory systems. Depending on the animal selected the energetic demands and flux through the oxygen transport system can increase two orders of magnitude from rest to maximal exercise. Thus, animal models in ... Read more >>

Am. J. Physiol. Heart Circ. Physiol. (American journal of physiology. Heart and circulatory physiology)
[2020, :]

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Imaging of the aortic root on high-pitch non-gated and ECG-gated CT: awareness is the key!

Prashant Nagpal, Mukta D Agrawal, Sachin S Saboo, Sandeep Hedgire, Sarv Priya, Michael L Steigner,

The aortic pathologies are well recognized on imaging. However, conventionally cardiac and proximal aortic abnormalities were only seen on dedicated cardiac or aortic studies due to need for ECG gating. Advances in CT technology have allowed motionless imaging of the chest and abdomen, leading to an increased visualization of cardiac ... Read more >>

Insights Imaging (Insights into imaging)
[2020, 11(1):51]

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First-ever ischemic stroke and increased risk of incident heart disease in older adults.

Luciano A Sposato, Melody Lam, Britney Allen, Lucie Richard, Salimah Z Shariff, Gustavo Saposnik,

OBJECTIVE:Poststroke cardiac complications are common. It is unknown whether the reason is shared risk factors and preexisting heart disease or stroke-associated myocardial and coronary injury. We tested the hypothesis that first-ever ischemic stroke is associated with increased risk of incident cardiovascular complications in patients without known preexisting cardiac comorbid conditions. ... Read more >>

Neurology (Neurology)
[2020, :]

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Cardiac ketone body metabolism.

Azrul Abdul Kadir, Kieran Clarke, Rhys D Evans,

The ketone bodies, d-β-hydroxybutyrate and acetoacetate, are soluble 4-carbon compounds derived principally from fatty acids, that can be metabolised by many oxidative tissues, including heart, in carbohydrate-depleted conditions as glucose-sparing energy substrates. They also have important signalling functions, acting through G-protein coupled receptors and histone deacetylases to regulate metabolism and ... Read more >>

Biochim Biophys Acta Mol Basis Dis (Biochimica et biophysica acta. Molecular basis of disease)
[2020, 1866(6):165739]

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Thanatogenomic Investigation of the Hydroxymethylome and Mitochondrial Genome of Cadaveric Cardiomyocytes: Proposal for a Proof-of-Concept Study.

Nerissa Naidoo, Gurjyot Bajwa, Ruthwik Duvuru, Yajnavalka Banerjee,

BACKGROUND:Cardiovascular disease (CVD) remains the leading cause of death in the United Arab Emirates (UAE). One of the common CVDs is hypertrophic cardiomyopathy (HCM). Recent studies conducted in heart cells of mice have shown that this condition involves a chemical modification called hydroxymethylation of the DNA of heart cells. OBJECTIVE:Objectives ... Read more >>

JMIR Res Protoc (JMIR research protocols)
[2020, 9(3):e17241]

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Morphometric Features of Patent Foramen Ovale as a Risk Factor of Cerebrovascular Accidents: A Systematic Review and Meta-Analysis.

Mateusz K Hołda, Mateusz Koziej,

INTRODUCTION:It is still disputable whether specific morphometric features of the patent foramen ovale (PFO) may stratify patients by the related probability that a discovered PFO is incidental or stroke related. OBJECTIVE:We aimed to determine whether certain morphometrical characteristics of PFO are associated with an increased risk of cerebrovascular accidents, using ... Read more >>

Cerebrovasc. Dis. (Cerebrovascular diseases (Basel, Switzerland))
[2020, 49(1):1-9]

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Pre-culture of mesenchymal stem cells within RGD-modified hyaluronic acid hydrogel improves their resilience to ischaemic conditions.

Laura B Gallagher, Eimear B Dolan, Janice O'Sullivan, Ruth Levey, Brenton L Cavanagh, Lenka Kovarova, Martin Pravda, Vladimir Velebny, Tom Farrell, Fergal J O'Brien, Garry P Duffy,

The incorporation of the RGD peptide (arginine-glycine-aspartate) into biomaterials has been proposed to promote cell adhesion to the matrix, which can influence and control cell behaviour and function. While many studies have utilised RGD modified biomaterials for cell delivery, few have examined its effect under the condition of reduced oxygen ... Read more >>

Acta Biomater (Acta biomaterialia)
[2020, :]

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Left Atrial Appendage Closure: Technical Considerations of Endocardial Closure.

Carlos E Sanchez, Steven J Yakubov, Anish Amin, Arash Arshi,

Left atrial appendage closure (LAAC) is a safe and effective therapy for the prevention of stroke in patients with nonvalvular atrial fibrillation and high bleeding risk with oral anticoagulants. Multimodality imaging with transesophageal echocardiography and computed tomography angiography to define the anatomy and its implications on endocardial exclusion is becoming ... Read more >>

Card Electrophysiol Clin (Cardiac electrophysiology clinics)
[2020, 12(1):47-54]

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