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Isolated THATCH domain of End4 is unable to bind F-actin independently in the fission yeast Schizosaccharomyces pombe.

Yuan Ren, Julien Berro,

Clathrin mediated endocytosis (CME) in the fission yeast <i>Schizosaccharomyces pombe</i> critically depends on the connection between the lipid membrane and F-actin. The fission yeast endocytic protein End4 (homologous to Sla2 in budding yeast and HIP1R in human) contains a N-terminal domain that binds to PIP2 on the membrane, and a ... Read more >>

MicroPubl Biol (microPublication biology)
[2022, 2022:]

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Cryopreservation of dehydrated Caenorhabditis elegans with multiple recoveries using a granular medium.

Julia Hayden, Christopher Fang-Yen,

Ultracold storage is widely used to preserve genetic stocks. Standard cryopreservation methods for the nematode <i>C. elegans</i> are vulnerable to refrigeration failures, which can result in the loss of stock viability due to freeze-thaw damage. In previous work our laboratory developed a method for cryopreserving worms in a dehydrated form ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Additional Landing Sites for Recombination-Mediated Cassette Exchange in C. elegans.

Michael Nonet,

Recombination-mediated cassette exchange (RMCE) is a recently developed alternative method for creating single copy transgenes using recombination rather than repair of double stranded breaks as the mechanism for driving integration into the genome. Two alternative methods for performing RMCE have been developed; a two-component approach using an unlinked source of ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Microscopy analysis of the smallest subunit of the RPA complex, Rfa3p, prompts consideration of how RPA subunits gather at single-stranded DNA sites.

Agnès Ramonatxo, María Moriel-Carretero,

The heterotrimeric Replication Protein A (RPA) complex preserves genome integrity by protecting the single-stranded DNA that becomes exposed during repair, replication, and recombination. Its two biggest subunits, Rfa1p and Rfa2p (as named in <i>S. cerevisiae</i>) contact DNA and interact with other partners, while the smallest Rfa3p subunit is considered to ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Overactive EGF signaling suppresses a C. elegans pnc-1 egg-laying phenotype independent of known signaling mediators.

Matt Crook, Wendy Hanna-Rose,

Nicotinamide recycling is critical to the development and function of <i>Caenorhabditis elegans</i>. Excess nicotinamide in a <i>pnc-1</i> nicotinamidase mutant causes the necrosis of uv1 and OLQ cells and a highly penetrant egg laying defect. An EGF receptor (<i>let-23</i>) gain-of-function mutation suppresses the Egl phenotype in <i>pnc-1</i> animals. However, gain-of-function mutations ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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SGS1-SuOff rescues the mild methylmethane sulfonate sensitivity of srs2Δ cells in Saccharomyces cerevisiae.

Belen Herce-Hagiwara, Yee Mon Thu,

Sgs1p in <i>Saccharomyces cerevisiae</i> belongs to the RecQ helicase family. Sgs1p is involved in recombination during DNA damage repair and sumoylation of Sgs1p is one mechanism by which the protein is regulated. To further understand the significance of Sgs1p sumoylation in DNA damage repair, we examined the genetic interaction between ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression.

Michael Nonet,

The first generation of <i>C. elegans</i> GAL4 drivers for bipartite expression function less well than <i>C. elegans</i> tet ON/OFF, QF and LexA drivers. The main difference between the GAL4 drivers and the others is the absence of a flexible linker between the DNA binding and activation domain in the GAL4 ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Suppression of him-14(it44ts) by a transgene insertion expressing GFP::COSA-1.

Chloé Girard, Chantal C Akerib, Anne M Villeneuve,

Meiotic crossover formation requires the activity of multiple pro-crossover factors, including the MutSγ complex and the cyclin-related protein COSA-1, that become concentrated together at the sites of crossover recombination intermediates. Here we show that a transgene insertion expressing GFP::COSA-1 can suppress the crossover deficit caused by a partial reduction in ... Read more >>

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[2021, 2021:]

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Live-cell imaging reveals square shape spindles and long mitosis duration in the silkworm holocentric cells.

Lucien Vanpoperinghe, Frederique Carlier-Grynkorn, Gaetan Cornilleau, Takahiro Kusakabe, Ines A Drinnenberg, Phong T Tran,

Proper chromosome segregation during mitosis requires both the assembly of a microtubule (MT)-based spindle and the assembly of DNA-centromere-based kinetochore structure. Kinetochore-to-MT attachment enables chromosome separation. Monocentric cells, such as found in human, have one unique kinetochore per chromosome. Holocentric cells, such as found in the silkworm, in contrast, have ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Dynein and MEL-28 contribute in parallel to oogenic maturity in C. elegans.

Jay Gandhi, Giulia Crosio, Anita G Fernandez,

<i>dhc-1(or283ts); mel-28(t1684)</i> double mutants have a severely reduced brood size compared to the wild-type and compared to each single mutant. To determine if this low-fecundity phenotype is associated with oocyte maturity defects, we used markers to assess the maturity of oocytes in the proximal gonad. We studied phosphorylated histone H3, ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Synergistic effects of hmp-2/β-catenin and sma-1H-spectrin on epidermal morphogenesis in Caenorhabditis elegans.

Sydney Wieberg, Harper Euwer, Anna Gerst, Stephanie L Maiden,

The F-actin, spectrin, and microtubule cytoskeletons are important mediators of embryonic epidermal morphogenesis in <i>Caenorhabditis elegans</i>. While SMA-1/β<sub>H</sub>-spectrin is known to organize actin bundles that connect to cadherin-based adhesions, the role of microtubules in the developing epidermis is not well understood. To determine if the spectrin cytoskeleton also plays a ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Saul-Wilson Syndrome Missense Allele Does Not Show Obvious Golgi Defects in a C. elegans Model.

Isabella Zafra, Benjamin Nebenfuehr, Andy Golden,

Saul-Wilson Syndrome is an ultra-rare skeletal syndrome caused by a mutation in the COG4 gene resulting in a glycine-to-arginine substitution at amino acid position 516. The COG4 gene encodes one of 8 subunits of the conserved oligomeric Golgi complex. Using CRISPR-Cas9, our lab generated a <i>C. elegans</i> model for Saul-Wilson ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Anti-oxidant MitoQ rescue of AWB chemosensory neuron impairment in a C. elegans model of X-linked Adrenoleukodystrophy.

Sanjib Guha, Aurora Pujol, Esther Dalfo,

X-linked Adrenoleukodystrophy (X-ALD) is a neurometabolic disorder caused by a defective peroxisomal ABCD1 transporter of very long-chain fatty acids (VLCFAs). We have characterized a nematode model of X-ALD with loss of the <i>pmp-4</i> gene, the worm orthologue of <i>ABCD1</i>. These mutants recapitulated the key hallmarks of X-ALD and importantly mitochondria ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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B.2.16 is a non-lethal modifier of the Dark82 mosaic eye phenotype in Drosophila melanogaster.

Alysia D Vrailas-Mortimer, Nitish Aggarwal, Neha N Ahmed, Ian M Alberts, Masa Alhawasli, Ibrahim A Aljerdi, Brooke M Allen, Ahmad M Alnajar, Michael A Anderson, Ryan Armstong, Chace C Avery, Eric J Avila, Tyra N Baker, Soolmaz Basardeh, Natasha A Bates, Farrah N Beidas, Ashton C Bosler, Deja M Brewer, Ryan S Buenaventura, Natalie Jl Burrell, Alyn P Cabrera-Lopez, Amairani B Cervantes-Gonzalez, Raymond P Cezar, Joselyn Coronel, Corinne Croslyn, Konnor R Damery, Lucero Diaz-Alavez, Nupur P Dixit, Dilcia L Duarte, Amanda R Emke, Katherine English, Audrey A Eshun, Samuel R Esterly, Arnold J Estrada, Mark Feng, Meghan M Freund, Niko Garcia, Charandeep S Ghotra, Haddia Ghyasi, Christa Sa Hale, Luci Hulsman, Lindsey Jamerson, Austin K Jones, Madeline Kuczynski, Takailah N Lacey-Kennedy, Mindy J Lee, Tala Mahjoub, Molly C Mersinger, Alyssa D Muckerheide, David W Myers, Karen Nielsen, Paul J Nosowicz, Jacinda A Nunez, Amy C Ortiz, Tulsi T Patel, Natalie N Perry, W Storm A Poser, Deisy M Puga, Cathrine Quam, Paulina Quintana-Lopez, Piper Rennerfeldt, Nicholas M Reyes, Ian G Rines, Cally Roberts, Daniel B Robinson, Kalina M Rossa, Gloria J Ruhlmann, Jeremy Schmidt, John R Sherwood, Dimyana H Shonoda, Hannah Soellner, Juan C Torrez, Mayukah Velide, Zachary Weinzapfel, Alisa C Ward, Kayla L Bieser, Julie A Merkle, Joyce C Stamm, Richard L Tillett, Jacob D Kagey,

Genetic screens have been used to identify genes involved in the regulation of different biological processes. We identified growth mutants in a Flp/FRT screen using the <i>Drosophila melanogaster</i> eye to identify conditional regulators of cell growth and cell division. One mutant identified from this screen, <i>B.2.16</i>, was mapped and characterized ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Conserved missense variant in ALDH1A3 ortholog impairs fecundity in C. elegans.

Wan-Rong Wong, Shayda Maher, Jun Young Oh, Katherine I Brugman, Shahla Gharib, Paul W Sternberg,

Accumulating evidence demonstrates that mutations in <i>ALDH1A3</i> (the aldehyde dehydrogenase 1 family, member A3) are associated with developmental defects. The ALDH1A3 enzyme catalyzes retinoic acid biosynthesis and is essential to patterning and neuronal differentiation in the development of embryonic nervous system. Several missense mutations in <i>ALDH1A3</i> have been identified in ... Read more >>

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[2021, 2021:]

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Ovarian somatic Piwi regulates nurse cell proliferation and oocyte specification in Drosophila.

Lauren E Gonzalez, Gina Zhu, Haifan Lin,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Expression of an endosome-excluded Cd63 prevents axis elongation in Xenopus.

Jennifer Kreis, Ramona Bonß, Kerstin Feistel, Philipp Vick,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The daf-2 insulin receptor functions in C. elegans embryo elongation.

Annu Suresh, Bruce Wightman,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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tmem-258 is dispensable for both nuclear anchorage and migration in C. elegans.

Ellen Gregory, Daniel Starr,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Caspar SUMOylation regulates Drosophila lifespan.

Bhagyashree Kaduskar, Deepti Trivedi, Girish S Ratnaparkhi,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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me101 is a new allele of rad-51.

Baptiste Roelens, Karl Zawadzki, Anne Villeneuve,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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Genetic mapping of EgfrL.3.1 in Drosophila melanogaster.

Joyce Stamm, Gnanda Joshi, M A Anderson, Katie Bussing, Colton Houchin, Amber Elinsky, Jacob Flyte, Nadine Husseini, Dominika Jarosz, Chelsea Johnson, Abby Johnson, Christina Jones, Taj Kooner, Daniel Myhre, Thomas Rafaill, Sarah Sayed, Kirby Swan, Jonathan Toma, Jacob Kagey,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

Cited: 4 times

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The mapping of Drosophila melanogaster mutant A.4.4.

Kayla Bieser, Joyce Stamm, Ayala Aldo, Suneil Bhaskara, Makayla Clairborne, Joselyn Coronel Gómez, Ron Dean, Aaron Dowell, Evan Dowell, Mathew Eissa, Ahmad Fawaz, Michael Fouad-Meshriky, Dustin Godoy, Krista Gonzalez, Malak Hachem, Malak Hammoud, Anthony Huffman, Hunter Ingram, Alex Jackman, Bibek Karki, Natalia Khalil, Houda Khalil, Tran Khanh Ha, Arjun Kharel, Izabell Kobylarz, Hunter Lomprey, Adam Lonnberg, Safa Mahbuba, Hend Massarani, Madeline Minster, Krystina Molina, Lynette Molitor, Taylor Murray, Payal Patel, Sydney Pechulis, Architha Raja, Gladys Rastegari, Skylar Reeves, Niveda Sabu, Rafael Salazar, Devan Schulert, Matthew Senopole, Kristen Sportiello, Claudia Torres, Jade Villalobos, Joseph Wu, Stacy Zeigler, Jacob Kagey,

MicroPubl Biol (microPublication biology)
[2018, 2018:]

Cited: 4 times

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Characterizing Dynein's Role in P-cell Nuclear Migration using an Auxin-Induced Degradation System.

Jamie Ho, Venecia A Valdez, Linda Ma, Daniel A Starr,

MicroPubl Biol (microPublication biology)
[2018, 2018:]

Cited: 0 times

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Screening for C. elegans male copulation-defective mutants by the mating plug phenotype.

Katharine Liu, Yvonne Hajdu-Cronin, Ann Chen, Gary Schindelman, Allyson Whittaker, Shahla Gharib, Paul Sternberg,

MicroPubl Biol (microPublication biology)
[2017, 2017:]

Cited: 2 times

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