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The effectiveness of EGFR knockdown by RNAi lines varies depending on the tissue.

Mikaela Follmer, Brandy Shrawder, Kara Eckert, Briana Heinly, Pavithra Vivekanand,

In <i>Drosophila</i> , the Epidermal growth factor receptor (EGFR) signaling pathway is known to be critically involved in multiple stages of development. We induced a loss of function phenotype in the eyes, wings, and somatic follicle cells using four EGFR RNAi lines: HMS05003 and JF02283, which produce short hairpin RNAs, ... Read more >>

MicroPubl Biol (microPublication biology)
[2022, 2022:]

Cited: 0 times

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Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains.

Samiksha Kaul, Han Ting Chou, Seleipiri Charles, Guillaume Aubry, Hang Lu, Annalise B Paaby,

Wild <i>C. elegans</i> strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryos of two distinct strains. We capture transcripts at high resolution by ... Read more >>

MicroPubl Biol (microPublication biology)
[2022, 2022:]

Cited: 0 times

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