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Direct comparisons of bisulfite pyrosequencing versus targeted bisulfite sequencing.

Dillon E King, A Clare Sparling, Rashmi Joglekar, Joel N Meyer, Susan K Murphy,

DNA methylation is an important epigenetic mechanism involved in proper genome function. Bisulfite pyrosequencing (PSQ) is a commonly used technique to quantify DNA methylation. Although very accurate, bisulfite pyrosequencing can be expensive and time consuming for large-scale quantitative DNA methylation analysis at the single nucleotide level. High throughput DNA methylation ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Annotating Putative D. discoideum Proteins Using I-TASSER.

Jacquelyn McCullough, Petra Fey, Ryan J Rahman, Morgan Wallace, Seeta Morey, Kyle Sahlberg, Ethan McGonagle, Danielle Hess, Chance Hatfield, Michaela-Romina Sarmiento, Jordi Velasquez, Richard H Gomer,

Using Gene Ontology annotation in any aspect or using any evidence code, we found that approximately 14% percent of predicted <i>D. discoideum</i> proteins have no GO annotations and no obvious similarity to any annotated protein across diverse organisms. We have been systematically examining these unannotated protein sequences using software that ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Time-to-enrollment in clinical trials investigating neurological recovery in chronic spinal cord injury: observations from a systematic review and ClinicalTrials.gov database.

F M Moinuddin, Yagiz Ugur Yolcu, Waseem Wahood, Jad Zreik, Sandy Goncalves, Anthony John Windebank, Wenchun Qu, Mohamad Bydon,

Currently, large numbers of clinical trials are performed to investigate different forms of experimental therapy for patients suffering from chronic spinal cord injury (SCI). However, for the enrollment process, there are different views on how the time period between injury and interventions should be determined. Herein, we sought to evaluate ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(5):953-958]

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Social skills and psychopathology are associated with autonomic function in children: a cross-sectional observational study.

Elisa Cainelli, Luca Vedovelli, Daniele Bottigliengo, Dario Boschiero, Agnese Suppiej,

In recent years, the increase of psychopathological disorders in the population has become a health emergency, leading to a great effort to understand psychological vulnerability mechanisms. In this scenario, the role of the autonomic nervous system (ANS) has become increasingly important. This study investigated the association between ANS, social skills, ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(4):920-928]

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Comparative analgesic, hemodynamic, pain and duration of sensory and motor block effects of dexmedetomidine, granisetron, and nitroglycerin added to ropivacaine in intravenous anesthesia for forearm surgeries: a randomized clinical study.

Esmail Moshiri, Hesameddin Modir, Alireza Kamali, Mehran Azami, Morteza Molouk,

This trial-based paper strives to address the comparative efficacy of some ropivacaine adjuvant options, comprising dexmedetomidine, granisetron, and nitroglycerin, on pain and hemodynamic changes in intravenous anesthesia for forearm surgeries. This double-blind, placebo-controlled study enrolled four block-randomized eligible groups with patients (overall, n=128) undergoing orthopedic forearm surgeries in the dexmedetomidine, ... Read more >>

Med Gas Res (Medical gas research)
[2022, 12(3):77-82]

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Risk of bias in observational studies using routinely collected data of comparative effectiveness research: a meta-research study.

Van Thu Nguyen, Mishelle Engleton, Mauricia Davison, Philippe Ravaud, Raphael Porcher, Isabelle Boutron,

<h4>Background</h4>To assess the completeness of reporting, research transparency practices, and risk of selection and immortal bias in observational studies using routinely collected data for comparative effectiveness research.<h4>Method</h4>We performed a meta-research study by searching PubMed for comparative effectiveness observational studies evaluating therapeutic interventions using routinely collected data published in high impact ... Read more >>

BMC Med (BMC medicine)
[2021, 19(1):279]

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Caregivers' report of preventive dental care visits and services for US WIC-participating and non-participating children.

Denise M Claiborne, Chun Chen, Qi Zhang,

To determine whether children participating in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) had more caregiver-reported preventive dental care visits (PDCVs) and preventive dental services (PDS) than nonparticipating children. A secondary data analysis was performed utilizing the National Survey of Children's Health 2017 and 2018 combined ... Read more >>

J Public Health Dent (Journal of public health dentistry)
[2021, :]

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Asthma biologic trial eligibility and real-world outcomes in the United States.

Regina W Lam, Jonathan W Inselman, Molly M Jeffery, Jacob T Maddux, Nilay D Shah, Matthew A Rank,

<b>Objective</b>: To compare the outcomes of real-world patients who would have been eligible for asthma biologics to those who would not have been eligible.<b>Methods:</b> We used data from the OptumLabs Data Warehouse (OLDW) to categorize patients into eligible and ineligible groups based on clinical trials (<i>n</i> = 19 trials) used for Food ... Read more >>

J Asthma (The Journal of asthma : official journal of the Association for the Care of Asthma)
[2021, :1-12]

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Differences in standardized neonatal encephalopathy exam criteria may impact therapeutic hypothermia eligibility.

Brian H Walsh, Hoda El-Shibiny, Chelsea Munster, Edward Yang, Terrie E Inder, Mohamed El-Dib,

<h4>Background</h4>Therapeutic hypothermia (TH) is routinely provided to those with moderate or severe neonatal encephalopathy (NE). Subtle differences exist in the standardized exams used to define NE severity. We aimed to assess if an infant's TH eligibility status differed if they were evaluated using either the NICHD/Neonatal Research Network's (NICHD-NRN) or ... Read more >>

Pediatr Res (Pediatric research)
[2021, :]

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Ultra-early amplitude decrement after repetitive nerve stimulation supports early neuromuscular junction injury in amyotrophic lateral sclerosis: a prospective cross-sectional study.

Jing-Yue Ma, Xiang-Yi Liu, Shuo Zhang, Dong-Sheng Fan,

The dying-back hypothesis holds that the damage to neuromuscular junctions and distal axons in amyotrophic lateral sclerosis occurs at the earliest stage of the disease. Previous basic studies have confirmed early damage to neuromuscular junctions, but it is difficult to obtain such evidence directly in clinical practice. In this prospective ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(3):655-660]

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Neutrophil-to-lymphocyte ratio in sporadic amyotrophic lateral sclerosis.

Qian-Qian Wei, Yan-Bing Hou, Ling-Yu Zhang, Ru-Wei Ou, Bei Cao, Yong-Ping Chen, Hui-Fang Shang,

The neutrophil-to-lymphocyte ratio (NLR) is considered a robust prognostic biomarker for predicting patient survival outcomes in many diseases. However, it remains unclear whether it can be used as a biomarker for amyotrophic lateral sclerosis (ALS). To correlate NLR with disease progression and survival in sporadic ALS, 1030 patients with ALS ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(4):875-880]

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Factors influencing performance on the Board Certified Psychiatric Pharmacist Examination: Passing rates and domain-level scores.

Timothy J Muckle, Julie Dopheide, Kelly Gable, Yu Meng, Samuel G Johnson, William Ellis,

The Board Certified Psychiatric Pharmacist (BCPP) specialty certification was launched by the Board of Pharmacy Specialties in 1994. Candidates for the BCPP can qualify for the examination through 3 possible pathways: practice experience (4 years) in the specialty, completion of a PGY-1 residency plus an additional 2 years of practice ... Read more >>

Ment Health Clin (The mental health clinician)
[2021, 11(6):358-364]

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The Line Between Medicaid and Marketplace: Coverage Effects from Wisconsin's Partial Expansion.

Laura Dague, Marguerite Burns, Donna Friedsam,

<h4>Context</h4>States have sought to experiment with the income eligibility threshold between Medicaid coverage and access to subsidized Marketplace plans in an effort to increase coverage for low-income adults while meeting other state priorities, particularly a balanced budget. In 2014, Wisconsin opted against adoption of an ACA Medicaid expansion, instead setting ... Read more >>

J Health Polit Policy Law (Journal of health politics, policy and law)
[2021, :]

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Clinical trial recruiters' experiences working with trial eligibility criteria: results of an exploratory, cross-sectional, online survey in the UK.

William J Cragg, Kathryn McMahon, Jamie B Oughton, Rachel Sigsworth, Christopher Taylor, Vicky Napp,

<h4>Background</h4>Eligibility criteria are a fundamental element of clinical trial design, defining who can and who should not participate in a trial. Problems with the design or application of criteria are known to occur and pose risks to participants' safety and trial integrity, sometimes also negatively impacting on trial recruitment and ... Read more >>

Trials (Trials)
[2021, 22(1):736]

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Eligibility of patients with heart failure with preserved ejection fraction for sacubitril/valsartan according to the PARAGON-HF trial.

Lars H Lund, Gianluigi Savarese, Ashwin Venkateshvaran, Lina Benson, Anna Lundberg, Erwan Donal, Jean-Claude Daubert, Emmanuel Oger, Cecilia Linde, Camilla Hage,

<h4>Aims</h4>In the heart failure (HF) with preserved ejection fraction (HFpEF) PARAGON-HF trial, sacubitril/valsartan vs. valsartan improved mortality/morbidity in patients with left ventricular ejection fraction (LVEF) below median (57%). We assessed eligibility for sacubitril/valsartan based on four scenarios.<h4>Methods and results</h4>Eligibility was assessed in the Karolinska-Rennes study (acute HFpEF, LVEF ≥ 45%, and N-terminal ... Read more >>

ESC Heart Fail (ESC heart failure)
[2021, :]

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A modeling analysis to compare eligibility strategies for lung cancer screening in Brazil.

Adalberto Miranda-Filho, Hadrien Charvat, Freddie Bray, Arn Migowski, Li C Cheung, Salvatore Vaccarella, Mattias Johansson, Andre L Carvalho, Hilary A Robbins,

<h4>Background</h4>Country-specific evidence is needed to guide decisions regarding whether and how to implement lung cancer screening in different settings. For this study, we estimated the potential numbers of individuals screened and lung cancer deaths prevented in Brazil after applying different strategies to define screening eligibility.<h4>Methods</h4>We applied the Lung Cancer Death Risk ... Read more >>

EClinicalMedicine (EClinicalMedicine)
[2021, 42:101176]

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HIV Prevention Program Eligibility Among Adolescent Girls and Young Women - Namibia, 2019.

Nickolas T Agathis, Francis B Annor, Rachel Coomer, Jennifer Hegle, Pragna Patel, Norbert Forster, Gabrielle O'Malley, Alison L Ensminger, Rahimisa Kamuingona, Helena Andjamba, Molisa Manyando, Greta M Massetti,

The U.S. President's Emergency Plan for AIDS Relief (PEPFAR) relies on comprehensive and reliable population data to implement interventions to reduce HIV transmission in high-incidence areas among populations disproportionately affected by the HIV epidemic. Adolescent girls and young women in sub-Saharan Africa account for a disproportionate number of new HIV ... Read more >>

MMWR Morb Mortal Wkly Rep (MMWR. Morbidity and mortality weekly report)
[2021, 70(45):1570-1574]

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Eligibility, Utilization, and Effectiveness of 17-Alpha Hydroxyprogesterone Caproate (17OHPC) in a Statewide Population-Based Cohort of Medicaid Enrollees.

Xi Wang, Stephanie M Garcia, Katherine S Kellom, Rupsa C Boelig, Meredith Matone,

<h4>Objectives</h4> The primary objective was to estimate the initiation and adherence rates of 17 α-hydroxyprogesterone caproate (17OHPC) among eligible mothers in a statewide population-based cohort of Medicaid enrollees. The secondary objectives were to (1) determine the association of maternal sociodemographic and clinical characteristics with 17OHPC utilization and (2) assess the real-world ... Read more >>

Am J Perinatol (American journal of perinatology)
[2021, :]

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Surgical antibiotic prophylaxis administration practices.

S Shrestha, K Hann, K W Y Kyaw, P Koju, M Khogali,

<h4>Setting</h4>A referral hospital in Kavre, Nepal.<h4>Objectives</h4>To assess 1) compliance with National Antibiotic Treatment Guidelines (NATG), specifically, whether the administration of surgical antibiotic prophylaxis (SAP) (initial dosing and redosing) was in compliance with NATG for patients who were and were not eligible, and 2) development of surgical site infections (SSIs) among ... Read more >>

Public Health Action (Public health action)
[2021, 11(Suppl 1):18-23]

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University students experience the COVID-19 induced shift to remote instruction.

Bob Ives,

The COVID-19 pandemic required an abrupt shift from face-to-face to online instruction for many students in higher education in the United States. Prior research has raised some concerns about both equitable access to online courses, and the quality of instruction in online courses compared to face-to-face courses. This survey study ... Read more >>

Int J Educ Technol High Educ (International journal of educational technology in higher education)
[2021, 18(1):59]

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Increased prevalence of irritable bowel syndrome in migraine patients: a systematic review and meta-analysis.

Wasit Wongtrakul, Nipith Charoenngam, Patompong Ungprasert,

<h4>Objective</h4>Even though evidence showing increased prevalence of irritable bowel syndrome (IBS) among migraine patients exists, it has not been well-established and the magnitude of association varies substantially across the studies. This study aimed to comprehensively compare the prevalence of IBS among migraineurs versus nonmigraineurs using the systematic review and the ... Read more >>

Eur J Gastroenterol Hepatol (European journal of gastroenterology & hepatology)
[2022, 34(1):56-63]

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HIV incidence and compliance with deferral criteria over three progressively shorter time deferrals for men who have sex with men in Canada.

Niamh Caffrey, Mindy Goldman, Lori Osmond, Qi-Long Yi, Wenli Fan, Sheila F O'Brien,

In Canada, the deferral for men who have sex with men (MSM) has been progressively reduced from a permanent deferral for MSM since 1977, to 5 years, 1 year, and, most recently, 3 months. We estimated human immunodeficiency virus (HIV) residual risk and compliance with the MSM time deferral after each change. Four ... Read more >>

Transfusion (Transfusion)
[2021, :]

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UK Women's Views of the Concepts of Personalised Breast Cancer Risk Assessment and Risk-Stratified Breast Screening: A Qualitative Interview Study.

Charlotte Kelley-Jones, Suzanne Scott, Jo Waller,

Any introduction of risk-stratification within the NHS Breast Screening Programme needs to be considered acceptable by women. We conducted interviews to explore women's attitudes to personalised risk assessment and risk-stratified breast screening. Twenty-five UK women were purposively sampled by screening experience and socioeconomic background. Interview transcripts were qualitatively analysed using ... Read more >>

Cancers (Basel) (Cancers)
[2021, 13(22):]

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Leukoaraiosis is associated with clinical symptom severity, poor neurological function prognosis and stroke recurrence in mild intracerebral hemorrhage: a prospective multi-center cohort study.

Tian-Qi Xu, Wei-Zhi Lin, Yu-Lan Feng, Fan-Xia Shen, Jie Chen, Wei-Wen Wu, Xiao-Dong Zhu, Lin Gu, Yi Fu,

Leukoaraiosis (LA) results from ischemic injury in small cerebral vessels, which may be attributable to decreased vascular density, reduced cerebrovascular angiogenesis, decreased cerebral blood flow, or microcirculatory dysfunction in the brain. In this study, we enrolled 357 patients with mild intracerebral hemorrhage (ICH) from five hospitals in China and analyzed ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(4):819-823]

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Clinical Characteristics and Outcomes of Very Elderly Patients With Atrial Fibrillation at High Bleeding Risk - The Fushimi AF Registry.

Yuya Ide, Hisashi Ogawa, Kenjiro Ishigami, Syuhei Ikeda, Kosuke Doi, Yasuhiro Hamatani, Akiko Fujino, Yoshimori An, Mitsuru Ishii, Moritake Iguchi, Nobutoyo Masunaga, Masahiro Esato, Hikari Tsuji, Hiromichi Wada, Koji Hasegawa, Mitsuru Abe, Gregory Y H Lip, Masaharu Akao, ,

<b><i>Background:</i></b> The ELDERCARE-AF trial demonstrated that low-dose edoxaban prevented stroke or systemic embolism (SE) in very elderly Japanese patients with non-valvular atrial fibrillation (NVAF) in whom standard oral anticoagulant therapy was inappropriate because of high bleeding risk. The aim of this study was to elucidate the characteristics and outcomes of ... Read more >>

Circ Rep (Circulation reports)
[2021, 3(11):629-638]

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