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Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans.

George Huang, Bailey de Jesus, Alex Koh, Sara Blanco, Aubrie Rettmann, Ella DeMott, Melynda Sylvester, Cassie Ren, Carrie Meng, Skye Waterland, Anita Rhodes, Persephone Alicea, Abbey Flynn, Daniel J Dickinson, Ryan Doonan,

Streamlined, selection-based CRISPR knock-in protocols for <i>C. elegans</i> were first introduced six years ago (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). Though these selection-based approaches are powerful, one drawback has been the requirement to inject large numbers of P0 worms (~30-60 per gene target). We have found that a ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Acute exposure of Nicotine during Drosophila puncture injury activates an epidermal wound response reaction.

Chrissy Cherenfant, Michelle T Juarez,

Epidermal wound reaction after injury can be visualized in <i>Drosophila melanogaster</i> embryos by transgenic fluorescent wound reporters. A "local" reaction is limited to the epidermal cells surrounding a wound site occurs in <i>wildtype</i> embryos. A "global" reaction extends beyond the wound site to all epidermal cells occurs in immune response ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Drosophila yakuba - Tsc1.

Bailey Lose, Abigail Myers, Savanah Fondse, Ian Alberts, Joyce Stamm, James J Youngblom, Chinmay P Rele, Laura K Reed,

Gene Model for <i>Tsc1</i> in the <i>Drosophila yakuba</i>'s DyakCAF1 assembly (GCA_000005975.1). ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Drosophila grimshawi - Rheb.

Chinmay P Rele, Jared Williams, Laura K Reed, James J Youngblom, Wilson Leung,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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sym-2 loss-of-function causes glutamatergic neurodegeneration after oxidative stress.

Veronica H Ryan, Anne C Hart,

Although some RNA-binding proteins are known to contribute to neurodegeneration, the genetic interaction between the genes encoding these proteins is unclear. Here, we examine the interaction between <i>sym-2</i>, the gene encoding an ortholog of hnRNPF and hnRNPH, and <i>hrpa-1</i>, the ortholog of of the gene encoding hnRNPA2, which when mutated ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Minimum number of synaptic vesicles for the initiation of a single action potential at C. elegans neuromuscular junction.

Lili Chen, Ya Wang, Shangbang Gao,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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ARENA-based activity profiling of tau and TDP-43 transgenic C. elegans.

Heather N Currey, Anna Malinkevich, Penny Melquist, Nicole F Liachko,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Calx, a sodium/calcium exchanger, may affect lifespan in Drosophila melanogaster.

Jung-Wan Mok, Hyunglok Chung, Kwang-Wook Choi,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Modified auxin improves the auxin-inducible degradation (AID) system for laid C. elegans embryos.

Takefumi Negishi, Masayo Asakawa, Masato Kanemaki, Hitoshi Sawa,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

Cited: 2 times

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Temperature-dependent mitochondrial-nuclear epistasis.

Enrique Cazares-Navarro, Joseph Ross,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

Cited: 0 times

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Caspar SUMOylation regulates Drosophila lifespan.

Bhagyashree Kaduskar, Deepti Trivedi, Girish S Ratnaparkhi,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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A CRISPR/Cas9-generated cdc-7 loss of function mutation does not cause temperature-dependent fertility defects.

Heather Currey, Nicole Liachko,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

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Profile of the RNA in exosomes from astrocytes and microglia using deep sequencing: implications for neurodegeneration mechanisms.

Hui-Min Xie, Xing Su, Feng-Yuan Zhang, Chao-Lun Dai, Rong-Hua Wu, Yan Li, Xiao-Xiao Han, Xing-Mei Feng, Bin Yu, Shun-Xing Zhu, Song-Lin Zhou,

Glial cells play an important role in signal transduction, energy metabolism, extracellular ion homeostasis and neuroprotection of the central nervous system. However, few studies have explained the potential effects of exosomes from glial cells on central nervous system health and disease. In this study, the genes expressed in exosomes from ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(3):608-617]

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Identification of key genes involved in axon regeneration and Wallerian degeneration by weighted gene co-expression network analysis.

Yan Lu, Qi Shan, Mei Ling, Xi-An Ni, Su-Su Mao, Bin Yu, Qian-Qian Cao,

Peripheral nerve injury repair requires a certain degree of cooperation between axon regeneration and Wallerian degeneration. Therefore, investigating how axon regeneration and degeneration work together to repair peripheral nerve injury may uncover the molecular mechanisms and signal cascades underlying peripheral nerve repair and provide potential strategies for improving the low ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(4):911-919]

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A Hybrid Reactive Multiphasic Mixture With a Compressible Fluid Solvent.

Jay J Shim, Gerard A Ateshian,

Mixture theory is a general framework that has been used to model mixtures of solid, fluid, and solute constituents, leading to significant advances in modeling the mechanics of biological tissues and cells. Though versatile and applicable to a wide range of problems in biomechanics and biophysics, standard multiphasic mixture frameworks ... Read more >>

J Biomech Eng (Journal of biomechanical engineering)
[2022, 144(1):]

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Post-residency medical education fellowships: a scoping review.

Mariel L Cataldi, Margot Kelly-Hedrick, Julie Nanavati, Margaret S Chisolm, Walsh Anne L,

The authors conducted a scoping review to investigate the structure, content, and potential impact of post-residency medical education fellowships. The authors searched eight databases to identify English-language articles describing longitudinal, post-residency medical fellowships that both focused on medical education and described the structure and content of the curriculum. The authors ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1920084]

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Satisfaction with online learning in the new normal: perspective of students and faculty at medical and health sciences colleges.

Wiam Elshami, Mohamed H Taha, Mohamed Abuzaid, Coumaravelou Saravanan, Sausan Al Kawas, Mohamed Elhassan Abdalla,

Online learning has become the new normal in many medical and health science schools worldwide, courtesy of COVID-19. Satisfaction with online learning is a significant aspect of promoting successful educational processes. This study aimed to identify factors affecting student and faculty satisfaction with online learning during the new normal. Online ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1920090]

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A quantitative study of letters to the editor by medical students in medical education journals.

Anvarjon Mukhammadaminov, Sanketh Rampes, Yasmin Amy Divecha, Laura Leeves, David Hammond, Azeem Alam, Russell Hearn,

Letters to the Editor offer ways for readers to engage with authors' publications. Letters are the shortest manuscript for medical students to publish and medical-education journals are best suited. The UK Foundation Programme rewards medical students achieving PubMed ID publications and we hypothesise that this is a main motivation for ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1912879]

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Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae.

Sadia Iqbal, John Fosu-Nyarko, Frances Brigg, Michael G K Jones,

Next-generation sequencing and analyses of whole-genome transcripts can be used to identify genes and potential mechanisms that may be responsible for the development of resistance to insecticides. Such genes can be identified by isolating and sequencing high-quality messenger RNA and identifying differentially expressed genes (DEGs), and gene variants from insecticide-treated ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2022, 2360:119-138]

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Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peach Aphid, Myzus persicae.

John Fosu-Nyarko, Sadia Iqbal, Frances Brigg, Michael G K Jones,

Identifying genes responsive to insecticide treatment is the first step towards understanding the mechanism(s) of insecticide resistance and the development of effective insecticides against economic insect pests such as the Green peach aphid (GPA). Functional and Reverse Genetics approaches such as the RNA interference (RNAi) technology can be used to ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2022, 2360:187-208]

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Polysubstance abuse among sexually abused in alcohol, drug, and gambling addiction treatment in Greenland: a cross sectional study.

Sara Viskum Leth, Maibritt Leif Bjerrum, Birgit V Niclasen,

This study aims to investigate the association between earlier sexual abuse and polysubstance abuse among persons in alcohol, drug and gambling addiction treatment in Greenland. The study included 431 individuals treated in 2017-2019. Data on exposure (sexual abuse), outcome (polysubstance abuse), and potential confounders (age, sex, school education, further education, ... Read more >>

Int J Circumpolar Health (International journal of circumpolar health)
[2021, 80(1):1849909]

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Twelve tips for teaching medical students online under COVID-19.

Zhehan Jiang, Hongbin Wu, Huaqin Cheng, Weimin Wang, A'Na Xie, Sarah Rose Fitzgerald,

Universities worldwide are pausing in an attempt to contain COVID-19's spread. In February 2019, universities in China took the lead, cancelling all in-person classes and switching to virtual classrooms, with a wave of other institutes globally following suit. The shift to online platform poses serious challenges to medical education so ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1854066]

Cited: 5 times

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Creating a 'choose your topic' massive open online course: an innovative and flexible approach to delivering injury prevention education.

Nadine I Ibrahim, Lauren Bohm, Jessica S Roche, Sarah A Stoddard, Rebecca M Quintana, Jennifer Vetter, Jeffrey Bennett, Beth Costello, Patrick M Carter, Rebecca Cunningham, Andrew N Hashikawa,

<h4>Background</h4>A pediatric injury prevention course has not been available as a massive open online course (MOOC). Creating a comprehensive topic course is particularly challenging because the traditional, week-by-week linear curriculum design is often a barrier to learners interested in only specific topics. We created a novel, flexible course as both ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1955646]

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Augmented reality in medical education: students' experiences and learning outcomes.

Poshmaal Dhar, Tetyana Rocks, Rasika M Samarasinghe, Garth Stephenson, Craig Smith,

Augmented reality (AR) is a relatively new technology that allows for digitally generated three-dimensional representations to be integrated with real environmental stimuli. AR can make use of smart phones, tablets, or other devices to achieve a highly stimulating learning environment and hands-on immersive experience. The use of AR in industry ... Read more >>

Med Educ Online (Medical education online)
[2021, 26(1):1953953]

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An In Vivo Data-Based Computational Study on Sitting-Induced Hemodynamic Changes in the External Iliac Artery.

Jixin Hou, Xuanyu Li, Zhaojun Li, Lekang Yin, Xin Chen, Fuyou Liang,

Although sedentary behavior (characterized by prolonged sitting without otherwise being active in daily life) is widely regarded as a risk factor for peripheral artery disease (PAD), underlying biomechanical mechanisms remain insufficiently understood. In this study, geometrical models of ten external iliac arteries were reconstructed based on angiographic data acquired from ... Read more >>

J Biomech Eng (Journal of biomechanical engineering)
[2022, 144(2):]

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