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An analog-sensitive allele of Aurora kinase B is lethal in mouse.

Berta N Vazquez, Suzanne M Quartuccio, Karen Schindler,

The mammalian genome encodes three Aurora protein kinase homologs (AURKA/B/C) which regulate chromosome segregation in nearly every cell type. AURKC expression is largely limited to meiotic cells. Because of the similarity in sequences between AURKB and AURKC, determining their separate functions during meiosis is challenging. We designed a chemical genetics ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Highly improved cloning efficiency for plasmid-based CRISPR knock-in in C. elegans.

Ella DeMott, Daniel J Dickinson, Ryan Doonan,

Plasmid-based CRISPR knock-in is a streamlined, scalable, and versatile approach for generating fluorescent protein tags in <i>C. elegans</i> (Dickinson <i>et al</i>. 2015; Schwartz and Jorgensen 2016). However, compared to more recent protocols that utilize commercially available Cas9/RNP products and linear DNA repair templates (Dokshin <i>et al</i>. 2018; Ghanta and Mello ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Identification and in silico analysis of the origin recognition complex in the human fungal pathogen Candida albicans.

Sreedevi Padmanabhan, Kaustuv Sanyal, Dharanidhar Dubey,

DNA replication in eukaryotes is initiated by the orchestrated assembly and association of initiator proteins (heterohexameric Origin Recognition Complex, ORC) on the replication origins. These functionally conserved proteins play significant roles in diverse cellular processes besides their central role in ignition of DNA replication at origins. <i>Candida albicans</i>, a major ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Deletion of a putative HDA-1 binding site in the hlh-2 promoter eliminates expression in C. elegans dorsal uterine cells.

Taylor N Medwig-Kinney, Nicholas J Palmisano, David Q Matus,

The helix-loop-helix transcription factor <i>hlh-2</i> (E/Daughterless) has been shown to play an important role in regulating cell fate patterning, cell cycle, and basement membrane invasion in the context of the development of the <i>C. elegans</i> somatic gonad. Here, using CRISPR/Cas9 genome engineering, we generated a new <i>hlh-2</i> allele (<i>hlh-2(Δ-1303-702)</i>) in ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila.

Masahiko Takemura, Yi-Si Lu, Eriko Nakato, Hiroshi Nakato,

Unpaired1 (Upd1) is a ligand of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway in <i>Drosophila</i>. In this study, using the CRISPR/Cas9 technique, we generate a transgenic fly strain in which a hemagglutinin (HA) epitope tag sequence is inserted into the endogenous locus of the <i>upd1</i> gene. ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Two plasmid modules for introducing the auxin-inducible degron into the fission yeast Schizosaccharomyces pombe by PCR-based gene targeting.

Xiuyi Song, Ruoming Xu, Tomoyasu Sugiyama,

Targeted protein degradation is a powerful approach to study and inhibit protein function <i>in vivo</i>. Introduction of the auxin-inducible degron (AID) system to the fission yeast <i>Schizosaccharomyces pombe</i> was previously reported, but, to the best of our knowledge, no plasmid for constructing AID-tagged fission yeast strains has been described so ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Efficient generation of a single-copy eft-3p::TIR1::F2A:: BFP::AID*::NLS allele in the C. elegans ttTi5605 insertion site through recombination-mediated cassette exchange.

An A Vo, Max T Levenson, James Matthew Ragle, Jordan D Ward,

The auxin-inducible degron (AID) system is a widely used system to conditionally deplete proteins. Using CRISPR/Cas9-based genome editing in <i>C. elegans</i>, we recently generated a set of single-copy, tissue-specific and pan-somatic TIR1-expressing strains carrying a BFP reporter inserted in single-copy into two commonly used, well-characterized genetic loci. However, we were ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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ATG8 is conserved between Saccharomyces cerevisiae and psychrophilic, polar-collected fungi.

Brenna J Ivory, Hannah M Smith, Elizabeth Cabrera, Meaghan R Robinson, Jackson T Sparks, Amanda Solem, Jun-Ichi Ishihara, Hiroki Takahashi, Masaharu Tsuji, Verónica A Segarra,

Autophagy is a conserved catabolic process by which eukaryotic cells respond to stress by targeting damaged or unneeded molecules or organelles for sequestration into specialized vesicles known as autophagosomes. Autophagosomes ultimately facilitate the digestion and recycling of their contents by fusing with the degradative organelle of the cell. Studies of ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Reducing the expression of the Numb adaptor protein in neurons increases the searching behavior of Drosophila larvae.

Andrew Galbraith, Samuel Leone, Katherine Stuart, Josie Emery, M Katie Renkemeyer, Nicholas Pritchett, Lauren Galbraith, Haley Stuckmeyer, Brett Berke,

<i>Drosophila</i> larval crawling is easily-observable and relatively stereotyped. Crawling consists of linear locomotion interrupted by periods when the larvae pause, swing their heads, and change direction (a 'search'). Here we identify Numb, a peripheral membrane adaptor protein, as an important regulator of searching behavior. When Numb RNAi transgenes were expressed ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Creation of a functional unc-11/PICALM GFP knock-in by CRISPR.

Maria Gallegos, Alex Hermes, Debjani Patra,

<i>unc-11</i> is the only <i>C. elegans</i> ortholog of mammalian PICALM/AP180, paralogs that play an important role in Clathrin-Mediated Endocytosis (CME) and the recycling of a subset of SNAREs, the vesicle-associated membrane proteins (VAMPs). In this publication we report the creation of a new <i>unc-11</i> allele that is endogenously-tagged with GFP ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Drosophila grimshawi - Rheb.

Chinmay P Rele, Jared Williams, Laura K Reed, James J Youngblom, Wilson Leung,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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The S1A protease family members CG10764 and CG4793 regulate cellular immunity in Drosophila.

Pooja Kr, Jonathan Lee, Nathan T Mortimer,

In nature, <i>Drosophila melanogaster</i> larvae are infected by parasitoid wasps and mount a cellular immune response to this infection. Several conserved signaling pathways have been implicated in coordinating this response, however our understanding of the integration and regulation of these pathways is incomplete. Members of the S1A serine protease family ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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CRISPRcruncher: A tool for engineering restriction sites into coding regions.

Samuel F Fay, David S Fay, Vikram E Chhatre,

CRISPR/Cas9 genome editing strategies often rely on the placement of an introduced restriction endonuclease (RE) site adjacent to the genomic edit of interest. This allows for rapid initial PCR-based detection of cells and organisms containing the edit of interest and may also be used for subsequent genotyping. Nevertheless, engineering RE ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Engineering essential genes with a "jump board" strategy using CRISPR/Cas9.

Ye Duan, Sungwook Choi, Charles Nelson, Victor Ambros,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Auxin does not affect a suite of morphological or behavioral phenotypes in two wild-type C. elegans strains.

Troy A McDiarmid, Lexis D Kepler, Catharine H Rankin,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Comparison of the efficiency of TIR1 transgenes to provoke auxin induced LAG-1 degradation in Caenorhabditis elegans germline stem cells.

Jian Chen, Ariz Mohammad, Tim Schedl,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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UMEPPI: An ultrasensitive detection method for protein-protein interaction.

Mikiya Umeyama, Jun Hirose, Kengo Morohashi,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Spontaneous neoplasia in the western clawed frog Xenopus tropicalis.

Makoto Suzuki, Takeshi Igawa, Nanoka Suzuki, Hajime Ogino, Haruki Ochi,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Net, skin and flatten, ImageJ plugin tool for extracting surface profiles from curved 3D objects.

Housei Wada, Shigeo Hayashi,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Quantitative insight into the combinatorial interactions within MYB-BHLH-WD complex in Arabidopsis thaliana.

Mikiya Umeyama, Kengo Morohashi,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Vitellogenin-2 acts downstream of PRY-1/Axin to regulate lipids and lifespan in C. elegans.

Avijit Mallick, Bhagwati P Gupta,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Regulation of sleep by KIN-29 is not developmental.

Jeremy J Grubbs, Alexander M van der Linden, David M Raizen,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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fs(1)A13041 is a 5' UTR deletion of the essential gene small ovary in Drosophila.

Myles Hammond, Jillian G Gomez, Brian Oliver, Steve Kucera, Leif Benner,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The mir-35-42 binding site in the nhl-2 3'UTR is dispensable for development and fecundity.

Bing Yang, Katherine McJunkin,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

Cited: 1 time

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The plant terpenoid carvone is a chemotaxis repellent for C. elegans.

Clayton Ellington, Andrew Hayden, Zack LaGrange, Marina Luccioni, Mohammed Osman, Lauren Ramlan, Miranda Vogt, Sujay Guha, Miriam Goodman, Lauren O'Connell,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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