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Using the Drosophila Transcriptional Reporter of Intracellular Calcium (TRIC) to examine lasting ethanol-induced changes in neuroexcitability.

Kahlan Merriman, Emily Petruccelli,

The <i>Drosophila</i> transcriptional reporter of intracellular calcium (TRIC) is a genetic tool used to measure lasting changes in neuroexcitability. Both pan-neuronal and dopaminergic cells were examined with TRIC to test the hypothesis that ethanol exposure causes lasting changes in adult brain neuroexcitability. We found little to no impact on TRIC ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Modeling human glucose-6-phosphate dehydrogenase mutations using C. elegans GSPD-1.

Luiza N Loges, Katherine M Walstrom,

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked, recessive condition that causes intermittent jaundice or hemolytic anemia because of low NADPH levels in red blood cells. We performed steady-state enzyme kinetics with the recombinant <i>C. elegans</i> ortholog of human G6PD, GSPD-1, and two mutants containing amino acid changes found in human ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Over-expression of the constitutive AMPylase FIC-1(E274G) does not deplete cellular ATP pools in C. elegans.

Margaret Champion, Matthias C Truttmann,

Protein AMPylation has emerged as a posttranslational protein modification regulating cellular proteostasis. AMPylation is conferred by Fic AMPylases, which catalyze the covalent attachment of AMP to target proteins at the expense of ATP. Over-expression of constitutive-active Fic AMPylases is toxic. Here, we test the hypothesis that excessive Fic AMPylase activity ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Methylation of the D2 dopamine receptor affects binding with the human regulatory proteins Par-4 and Calmodulin.

Alexander Bowitch, Ansuman Sahoo, Andrea M Clark, Christiana Ntangka, Krishna K Raut, Paul Gollnick, Michael C Yu, Steven M Pascal, Sarah E Walker, Denise M Ferkey,

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Serum-free adapted Drosophila S2R+ line is amenable to RNA interference.

Arthur Luhur, Daniel Mariyappa, Kristin M Klueg, Stephen L Rogers, Andrew C Zelhof,

We have previously adapted a select number of <i>Drosophila</i> cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against <i>Rho1</i> ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Purified Bighead protein efficiently promotes head development in the South African clawed frog, Xenopus laevis.

Gabriele Colozza,

Vertebrate embryonic development is regulated by a few families of extracellular signaling molecules. <i>Xenopus laevis</i> embryos offer an excellent system to study the cell-cell communication signals that govern embryonic patterning. In the frog embryos, Wnt/β-catenin plays a pivotal role in regulating embryonic axis development, and modulation of the Wnt pathway ... Read more >>

MicroPubl Biol (microPublication biology)
[2021, 2021:]

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Expression of an endosome-excluded Cd63 prevents axis elongation in Xenopus.

Jennifer Kreis, Ramona Bonß, Kerstin Feistel, Philipp Vick,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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The tetraspanin Cd63 is required for eye morphogenesis in Xenopus.

Jennifer Kreis, Ramona Bonß, Philipp Vick,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Phenotypic modulation of pentylenetetrazole-induced convulsive behaviors in C. elegans carrying a mutation associated with Alzheimer's disease.

Madeline A Vaji, Guy A Caldwell, Kim A Caldwell,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Evidence of slightly increased Pol II pausing in UPF1-depleted Drosophila melanogaster cells.

Anand K Singh, Jie Zhang, Daniel Hebenstreit, Saverio Brogna,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Zebrafish Trak proteins 1a and 2 localize to the mitochondria.

Kelsey A Oonk, Lauren B Bienvenu, Paxton S Sickler, Christine Martin, Emily Nickoloff-Bybel, Albert Matthew Volk, Douglas C Weiser, Susan Walsh,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Single-cell resolution long-term luciferase imaging in cultivated Drosophila brains.

Frank K Schubert, Charlotte Helfrich-Förster, Dirk Rieger,

MicroPubl Biol (microPublication biology)
[2020, 2020:]

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Identification of four Drosophila Toll-related proteins as ligands for the PTP69D receptor tyrosine phosphatase.

Namrata Bali, Hyung-Kook Lee, Kai Zinn,

MicroPubl Biol (microPublication biology)
[2019, 2019:]

Cited: 1 time

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Novel deletion alleles of a C. elegans gene Y48E1C.1, named as tm5468, tm5625 and tm5626.

Sayaka Hori, Yuji Suehiro, Sawako Yoshina, Shohei Mitani,

MicroPubl Biol (microPublication biology)
[2017, 2017:]

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Pre-clinical study of human umbilical cord mesenchymal stem cell transplantation for the treatment of traumatic brain injury: safety evaluation from immunogenic and oncogenic perspectives.

Gang Wang, Hua-Ling Wu, Yue-Ping Liu, De-Qi Yan, Zi-Lin Yuan, Li Chen, Qian Yang, Yu-Song Gao, Bo Diao,

Stem cell therapy is a promising strategy for the treatment of traumatic brain injury (TBI). However, animal experiments are needed to evaluate safety; in particular, to examine the immunogenicity and tumorigenicity of human umbilical cord mesenchymal stem cells (huMSCs) before clinical application. In this study, huMSCs were harvested from human ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(2):354-361]

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A pooled testing system to rapidly identify cattle carrying the elite controller BoLA-DRB3*009:02 haplotype against bovine leukemia virus infection.

Kosuke Notsu, Hala El Daous, Shuya Mitoma, Junzo Norimine, Satoshi Sekiguchi,

<h4>Background</h4>As genetically resistant individuals, the "elite controllers" (ECs) of human immunodeficiency virus infection have been focused on as the keys to developing further functional treatments in medicine. In the livestock production field, identifying the ECs of bovine leukemia virus (BLV) infection in cattle is desired to stop BLV transmission chains ... Read more >>

HLA (HLA)
[2021, :]

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Association between BoLA-DRB3 polymorphism and bovine leukemia virus proviral load in Vietnamese Holstein Friesian cattle.

Dung Le Thi, Son Nguyen Vu, Chieh-Wen Lo, Tung Duy Dao, Vuong Nghia Bui, Haruko Ogawa, Kunitoshi Imai, Katsuaki Sugiura, Yoko Aida, Takeshi Haga,

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis. Polymorphism in bovine leukocyte antigen (BoLA)-DRB3 allele can influence the host immune response to pathogens, including BLV. However, association between specific BoLA-DRB3 alleles and BLV proviral load (PVL), which is a useful index for estimating disease progression and ... Read more >>

HLA (HLA)
[2021, :]

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Different impact of bovine complement regulatory protein 46 (CD46bov) as a cellular receptor for members of the species Pestivirus H and Pestivirus G.

Elena Leveringhaus, Gökce Nur Cagatay, Juliane Hardt, Paul Becher, Alexander Postel,

The genus <i>Pestivirus</i> within the family <i>Flaviviridae</i> comprises highly relevant animal pathogens such as bovine viral diarrhoea virus 1 and 2 (BVDV-1 and -2) classified into the two species <i>Pestivirus A</i> and <i>Pestivirus B</i>, respectively. First described in 2004, HoBi-like pestiviruses (HoBiPeV) represent emerging bovine pathogens that belong to a ... Read more >>

Emerg Microbes Infect (Emerging microbes & infections)
[2021, :1-38]

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Prediction of molecular markers of bovine mastitis by meta-analysis of differentially expressed genes using combined p-value and robust rank aggregation

Anushri Umesh, Praveen Kumar Guttula, Mukesh Kumar Gupta,

Bovine mastitis causes significant economic loss to the dairy industry by affecting milk quality and quantity. E.coli and S.aureus are the two common mastitis-causing bacteria among the consortia of mastitis pathogens, wherein E.coli is an opportunistic environmental pathogen, and S.aureus is a contagious pathogen. This study was designed to predict ... Read more >>

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Preparation of Surface Plasmon Resonance Aptasensor for Human Activated Protein C Sensing.

Semra Akgönüllü, Seda Koyun, Handan Yavuz, Arzum Erdem, Adil Denizli,

Nucleic acid aptamers are an emerging class of artificial ligands and have recently gained attention in several areas. Here we report the design of a surface plasmon resonance (SPR) aptasensor for highly sensitive and selective sensing of human activated protein C (APC). First, DNA aptamer (DNA-Apt) specific for APC is ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2022, 2393:37-56]

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Lesion-induced changes of brevican expression in the perineuronal net of the superior vestibular nucleus.

Agnes Magyar, Eva Racz, Clara Matesz, Ervin Wolf, Peter Kiss, Botond Gaal,

Damage to the vestibular sense organs evokes static and dynamic deficits in the eye movements, posture and vegetative functions. After a shorter or longer period of time, the vestibular function is partially or completely restored via a series of processes such as modification in the efficacy of synaptic inputs. As ... Read more >>

Neural Regen Res (Neural regeneration research)
[2022, 17(3):649-654]

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Gold nanoclusters performing as contrast agents for non-invasive imaging of tissue-like phantoms via two-photon excited fluorescence lifetime imaging.

Alexandru-Milentie Hada, Ana-Maria Craciun, Simion Astilean,

Recently, gold nanoclusters (AuNCs) have received considerable scientific interest due to their ability to generate intrinsic photoluminescence (PL), making them suitable for a wide range of applications, such as sensing, biolabeling and bioimaging. Fluorescence lifetime imaging microscopy (FLIM) is an extremely promising technique when it comes to tissue imaging, especially ... Read more >>

Analyst (The Analyst)
[2021, 146(23):7126-7130]

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Bovine serum albumin as a resuscitation promoting factor for viable but non-culturable Mycobacterium tuberculosis via the activation of protein kinase A-dependent cellular processes

Yuta Morishige, Yoshiro Murase, Kinuyo Chikamatsu, Akio Aono, Yuriko Igarashi, Yoshiko Shimomura, Makiko Hosoya, Keisuke Kamada, Hiroyuki Yamada, Akiko Takaki, Satoshi Mitarai,

<h4>Objective</h4> Mycobacterium tuberculosis (Mtb) H37Ra strain has been reported to rapidly enter the viable but non-culturable (VBNC) state following treatment with an NADH oxidase inhibitor (diphenyleneiodonium [DPI]) and to be resuscitated by fetal bovine serum (FBS). However, the mechanism underlying FBS-induced resuscitation is currently unclear. We tried to reveal the ... Read more >>

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MiR-33a plays an crucial role in the proliferation of bovine preadipocytes.

Wenzhen Zhang, Li Wang, Sayed Haidar Abbas Raza, Xiaoyu Wang, Guohu Wang, Chengcheng Liang, Gong Cheng, Bingzhi Li, Linsen Zan,

Preadipocyte proliferation is a critical and precisely orchestrated procedure in adipogenesis, which is highly regulated by microRNAs (miRNAs). A previous study identified that the expression of miR-33a is different in intramuscular fat (IMF) tissues from steers and bulls. In the present study, miR-33a was overexpressed in bovine preadipocytes, and a ... Read more >>

Adipocyte (Adipocyte)
[2021, 10(1):189-200]

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Monitoring the interactions between bovine serum albumin and ZnO/Ag nanoparticles by spectroscopic techniques.

Abhishek Udnoor, Manjunath Lokolkar, Basappa C Yallur, Raju Kale, Muttanagoud N Kalasad, Umesha Katrahalli, Devagondanahalli H Manjunatha,

Inducing the bio-functionalization in noble metal nanoparticles like gold, silver, zinc is very important to accomplish their biocompatibility in biological activities. These metal nanoparticles are being rigorously used in bio-sensing tools keeping their remarkable properties in mind. Amongst the serum albumins, the most ample proteins in plasma are bovine serum ... Read more >>

J Biomol Struct Dyn (Journal of biomolecular structure & dynamics)
[2021, :1-14]

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