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Author Xiaoying Bian

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[Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva].

Wentao Zheng, Xue Wang, Yuwei Chen, Yachao Dong, Diao Zhou, Ruxin Liu, Haibo Zhou, Xiaoying Bian, Hailong Wang, Qiang Tu, Vinothkannan Ravichandran, Youming Zhang, Aiying Li, Jun Fu, Jia Yin,

Microbial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva CRS01-1 is a broad-spectrum antagonistic bacterium present in plants. However, no natural products have been isolated from this strain till date. Corresponding biosynthetic gene clusters to natural products ... Read more >>

Biotechnol J (Biotechnology journal)
[2021, :e2000575]

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Engineering and elucidation of the lipoinitiation process in nonribosomal peptide biosynthesis.

Lin Zhong, Xiaotong Diao, Na Zhang, Fengwei Li, Haibo Zhou, Hanna Chen, Xianping Bai, Xintong Ren, Youming Zhang, Dalei Wu, Xiaoying Bian,

Nonribosomal peptide synthetases containing starter condensation domains direct the biosynthesis of nonribosomal lipopeptides, which generally exhibit wide bioactivities. The acyl chain has strong impacts on bioactivity and toxicity, but the lack of an in-depth understanding of starter condensation domain-mediated lipoinitiation limits the bioengineering of NRPSs to obtain novel derivatives with ... Read more >>

Nat Commun (Nature communications)
[2021, 12(1):296]

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Reassembly of the Biosynthetic Gene Cluster Enables High Epothilone Yield in Engineered Schlegelella brevitalea.

Yucong Yu, Huimin Wang, Biao Tang, Junheng Liang, Lin Zhang, Hongkuan Wang, Xiaoying Bian, Yue-Zhong Li, Youming Zhang, Guo-Ping Zhao, Xiaoming Ding,

Epothilones, as a new class of microtubule-stabilizing anticancer drugs, exhibit strong bioactivity against taxane-resistant cells and show clinical activity for the treatment of advanced breast cancer. Additionally, they also show great potential for a central nervous system injury and Alzheimer's disease. However, due to the long fermentation period of the ... Read more >>

ACS Synth Biol (ACS synthetic biology)
[2020, 9(8):2009-2022]

Cited: 1 time

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Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.

Wentao Zheng, Xue Wang, Haibo Zhou, Youming Zhang, Aiying Li, Xiaoying Bian,

The Burkholderiales are an emerging source of bioactive natural products. Their genomes contain a large number of cryptic biosynthetic gene clusters (BGCs), indicating great potential for novel structures. However, the lack of genetic tools for the most of Burkholderiales strains restricts the mining of these cryptic BGCs. We previously discovered ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(2):397-405]

Cited: 1 time

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Attenuation of Pseudomonas aeruginosa Quorum Sensing by Natural Products: Virtual Screening, Evaluation and Biomolecular Interactions.

Lin Zhong, Vinothkannan Ravichandran, Na Zhang, Hailong Wang, Xiaoying Bian, Youming Zhang, Aiying Li,

Natural products play vital roles against infectious diseases since ancient times and most drugs in use today are derived from natural sources. Worldwide, multi-drug resistance becomes a massive threat to the society with increasing mortality. Hence, it is very crucial to identify alternate strategies to control these 'super bugs'. <i>Pseudomonas ... Read more >>

Int J Mol Sci (International journal of molecular sciences)
[2020, 21(6):]

Cited: 1 time

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Effect of the nitrogen-fixing bacterium Pseudomonas protegens CHA0-ΔretS-nif on garlic growth under different field conditions

Mei Wang, Zhilong Bian, Jing Shi, Yuxia Wu, Xu Yu, Yan Yang, Haiping Ni, Hanna Chen, Xiaoying Bian, Tao Li, Youming Zhang, Lihua Jiang, Qiang Tu,

Pseudomonas protegens CHA0 is a well-characterized plant-growth promoting rhizobacterium commonly used as biofertilizer or biocontrol agent for plant growth promotion and disease control. Genetically engineered strain CHA0-ΔretS-nif with introduced nitrogen-fixing ability and enhanced bactericidal activity has the potential to stimulate plant growth and suppress phytopathogens. Field experiments was conducted to ... Read more >>

(Industrial crops and products.)
[2020, 145:Not Available]

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Identification of Holrhizins E-Q Reveals the Diversity of Nonribosomal Lipopeptides in Paraburkholderia rhizoxinica.

Hanna Chen, Haibo Zhou, Tao Sun, Jiaying Xu, Qiang Tu, Jie Yang, Youming Zhang, Xiaoying Bian,

The products of a nonribosomal peptide synthetase gene, holA, from Paraburkholderia rhizoxinica were investigated using our recently established recombineering technique. Fifteen products, including 13 new linear lipopeptides, holrhizins E-Q (2-8, 10-15), together with the two known holrhizins A and B (1, 9), were detected in the activated mutant, and their ... Read more >>

J Nat Prod (Journal of natural products)
[2020, 83(2):537-541]

Cited: 1 time

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Genome Mining and Biosynthesis of Primary Amine-Acylated Desferrioxamines in a Marine Gliding Bacterium.

Zong-Jie Wang, Haibo Zhou, Guannan Zhong, Liujie Huo, Ya-Jie Tang, Youming Zhang, Xiaoying Bian,

Genome mining of <i>Fulvivirga</i> sp. W222 revealed a desferrioxamine-like biosynthetic gene cluster containing an unknown gene <i>fulF</i> that is conserved in many <i>Bacteroidetes</i> species. A series of primary amine-acylated desferrioxamine G<sub>1</sub> analogues, fulvivirgamides, were identified, and fulvivirgamides A<sub>2</sub>, B<sub>2</sub>, B<sub>3</sub>, and B<sub>4</sub> (<b>1</b>-<b>4</b>) were purified and characterized. The function of ... Read more >>

Org Lett (Organic letters)
[2020, 22(3):939-943]

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Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways.

Muhammad Nazeer Abbasi, Jun Fu, Xiaoying Bian, Hailong Wang, Youming Zhang, Aiying Li,

Microbial genomes encode many cryptic and uncharacterized biosynthetic gene clusters (BGCs). Exploiting this unexplored genetic wealth to discover microbial novel natural products (NPs) remains a challenging issue. We review homologous recombination (HR)-based recombineering, mediated by the recombinases RecE/RecT from Rac prophage and Redα/Redβ from lambda phage, which has developed into ... Read more >>

Trends Biotechnol (Trends in biotechnology)
[2020, 38(7):715-728]

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Promoter Screening Facilitates Heterologous Production of Complex Secondary Metabolites in Burkholderiales Strains.

Qing Ouyang, Xue Wang, Na Zhang, Lin Zhong, Jiaqi Liu, Xiaoming Ding, Youming Zhang, Xiaoying Bian,

Burkholderiales are an emerging source of bioactive secondary metabolites and have the potential to be a robust chassis for metabolites from Gram-negative bacteria. However, only a few constitutive promoters can be utilized in Burkholderiales. Herein, we described the screening of strong constitutive promoters from Burkholderiales strain DSM 7029, and 37 ... Read more >>

ACS Synth Biol (ACS synthetic biology)
[2020, 9(2):457-460]

Cited: 1 time

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Heterologous expression of bacterial natural product biosynthetic pathways.

Liujie Huo, Joachim J Hug, Chengzhang Fu, Xiaoying Bian, Youming Zhang, Rolf Müller,

Covering: 2013 to June 2018 Heterologous expression of natural product biosynthetic pathways is of increasing interest in microbial biotechnology, drug discovery and optimization. It empowers not only the robust production of valuable biomolecules in more amenable heterologous hosts but also permits the generation of novel analogs through biosynthetic engineering. This ... Read more >>

Nat Prod Rep (Natural product reports)
[2019, 36(10):1412-1436]

Cited: 12 times

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Reclassification of 'Polyangium brachysporum' DSM 7029 as Schlegelella brevitalea sp. nov.

Biao Tang, Yucong Yu, Junheng Liang, Youming Zhang, Xiaoying Bian, Xiaoyang Zhi, Xiaoming Ding,

Strain DSM 7029, isolated from a soil sample in Greece, can produce antitumour glidobactins, and has been found, as a heterologous host, to produce useful nonribosomal peptide synthetase-polyketide synthase hybrid molecules known as epothilones. This strain was originally named 'Polyangium brachysporum' of the family Polyangiaceae and the order Myxococcales. However, ... Read more >>

Int J Syst Evol Microbiol (International journal of systematic and evolutionary microbiology)
[2019, 69(9):2877-2883]

Cited: 3 times

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Biosynthesis of polyketides by trans-AT polyketide synthases in Burkholderiales.

Hanna Chen, Zhilong Bian, Vinothkannan Ravichandran, Ruijuan Li, Yi Sun, Liujie Huo, Jun Fu, Xiaoying Bian, Liqiu Xia, Qiang Tu, Youming Zhang,

Widely used as drugs and agrochemicals, polyketides are a family of bioactive natural products, with diverse structures and functions. Polyketides are produced by megaenzymes termed as polyketide synthases (PKSs). PKS biosynthetic pathways are divided into the <i>cis</i>-AT PKSs and <i>trans</i>-AT PKSs; a division based mainly on the absence of an ... Read more >>

Crit Rev Microbiol (Critical reviews in microbiology)
[2019, 45(2):162-181]

Cited: 0 times

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Expressing cytotoxic compounds in Escherichia coli Nissle 1917 for tumor-targeting therapy.

Ruijuan Li, Linda Helbig, Jun Fu, Xiaoying Bian, Jennifer Herrmann, Michael Baumann, A Francis Stewart, Rolf Müller, Aiying Li, Daniel Zips, Youming Zhang,

Abnormal blood vessels and hypoxic and necrotic regions are common features of solid tumors and related to the malignant phenotype and therapy resistance. Certain obligate or facultative anaerobic bacteria exhibit inherent ability to colonize and proliferate within solid tumors in vivo. Escherichia coli Nissle 1917 (EcN), a non-pathogenic probiotic in European ... Read more >>

Res Microbiol (Research in microbiology)
[2019, 170(2):74-79]

Cited: 6 times

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Engineering Pseudomonas protegens Pf-5 to improve its antifungal activity and nitrogen fixation.

Xiaoshu Jing, Qingwen Cui, Xiaochen Li, Jia Yin, Vinothkannan Ravichandran, Deng Pan, Jun Fu, Qiang Tu, Hailong Wang, Xiaoying Bian, Youming Zhang,

In agricultural production, sustainability is currently one of the most significant concerns. The genetic modification of plant growth-promoting rhizobacteria may provide a novel way to use natural bacteria as microbial inoculants. In this study, the root-colonizing strain Pseudomonas protegens Pf-5 was genetically modified to act as a biocontrol agent and ... Read more >>

Microb Biotechnol (Microbial biotechnology)
[2020, 13(1):118-133]

Cited: 0 times

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Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency.

Fangnan Yu, Xiaoshu Jing, Xiaochen Li, Hailong Wang, Hanna Chen, Lin Zhong, Jia Yin, Deng Pan, Yulong Yin, Jun Fu, Liqiu Xia, Xiaoying Bian, Qiang Tu, Youming Zhang,

Pseudomonas protegens CHA0 is a well-characterized, root-colonizing bacterium with broad-spectrum biocontrol ability. Therefore, it has a great potential to curb plant diseases and to partly replace synthetic chemical pesticides that are harmful to humans. Here, we obtained the multifunctional mutant CHA0-ΔretS-Nif via Red/ET recombineering technology. After deletion of the retS ... Read more >>

Microbiol Res (Microbiological research)
[2019, 218:58-65]

Cited: 1 time

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Yield improvement of epothilones in Burkholderia strain DSM7029 via transporter engineering.

Chenlang Liu, Fangnan Yu, Qingshu Liu, Xiaoying Bian, Shengbiao Hu, Huansheng Yang, Yulong Yin, Yuezhong Li, Yuemao Shen, Liqiu Xia, Qiang Tu, Youming Zhang,

Transporter engineering has been shown to be a positive approach for enhancing natural product titers in microbial cell factories by expelling target compounds out of feasible hosts. In this work, two multidrug efflux pumps, Orf14 and Orf3, were modulated in the epothilone production strain Burkholderia DSM7029::Tn5-km-epo (named G32) via Red/ET ... Read more >>

FEMS Microbiol Lett (FEMS microbiology letters)
[2018, 365(9):]

Cited: 2 times

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Heterologous Expression Guides Identification of the Biosynthetic Gene Cluster of Chuangxinmycin, an Indole Alkaloid Antibiotic.

Xiaokun Xu, Haibo Zhou, Yang Liu, Xiaotong Liu, Jun Fu, Aiying Li, Yue-Zhong Li, Yuemao Shen, Xiaoying Bian, Youming Zhang,

The indole alkaloid antibiotic chuangxinmycin, from Actinobacteria Actinoplanes tsinanensis, containing a unique thiopyrano[4,3,2- cd]indole scaffold, is a potent and selective inhibitor of bacterial tryptophanyl-tRNA synthetase. The chuangxinmycin biosynthetic gene cluster was identified by in silico analysis of the genome sequence, then verified by heterologous expression. Systemic gene inactivation and intermediate ... Read more >>

J Nat Prod (Journal of natural products)
[2018, 81(4):1060-1064]

Cited: 3 times

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Identification of a contact-dependent growth inhibition system in the probiotic Escherichia coli Nissle 1917.

Hanna Chen, Qian Fang, Qiang Tu, Chenlang Liu, Jia Yin, Yulong Yin, Liqiu Xia, Xiaoying Bian, Youming Zhang,

Contact-dependent growth inhibition (CDI) is a type of competitive mechanisms and has been identified in various strains including Burkholderia, Dickeya, E. coli and Yersinia. Classical CDI systems contain three genes, cdiB, cdiA and cdiI. CdiB encoded by cdiB gene is a conserved β-barrel protein and required for export of CdiA. ... Read more >>

FEMS Microbiol Lett (FEMS microbiology letters)
[2018, 365(11):]

Cited: 1 time

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Discovery of recombinases enables genome mining of cryptic biosynthetic gene clusters in Burkholderiales species.

Xue Wang, Haibo Zhou, Hanna Chen, Xiaoshu Jing, Wentao Zheng, Ruijuan Li, Tao Sun, Jiaqi Liu, Jun Fu, Liujie Huo, Yue-Zhong Li, Yuemao Shen, Xiaoming Ding, Rolf Müller, Xiaoying Bian, Youming Zhang,

Bacterial genomes encode numerous cryptic biosynthetic gene clusters (BGCs) that represent a largely untapped source of drugs or pesticides. Mining of the cryptic products is limited by the unavailability of streamlined genetic tools in native producers. Precise genome engineering using bacteriophage recombinases is particularly useful for genome mining. However, recombinases ... Read more >>

Proc Natl Acad Sci U S A (Proceedings of the National Academy of Sciences of the United States of America)
[2018, 115(18):E4255-E4263]

Cited: 8 times

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Gaze Patterns in Auditory-Visual Perception of Emotion by Children with Hearing Aids and Hearing Children.

Yifang Wang, Wei Zhou, Yanhong Cheng, Xiaoying Bian,

This study investigated eye-movement patterns during emotion perception for children with hearing aids and hearing children. Seventy-eight participants aged from 3 to 7 were asked to watch videos with a facial expression followed by an oral statement, and these two cues were either congruent or incongruent in emotional valence. Results ... Read more >>

Front Psychol (Frontiers in psychology)
[2017, 8:2281]

Cited: 0 times

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Heterologous Production and Yield Improvement of Epothilones in Burkholderiales Strain DSM 7029.

Xiaoying Bian, Biao Tang, Yucong Yu, Qiang Tu, Frank Gross, Hailong Wang, Aiying Li, Jun Fu, Yuemao Shen, Yue-Zhong Li, A Francis Stewart, Guoping Zhao, Xiaoming Ding, Rolf Müller, Youming Zhang,

The cloning of microbial natural product biosynthetic gene clusters and their heterologous expression in a suitable host have proven to be a feasible approach to improve the yield of valuable natural products and to begin mining cryptic natural products in microorganisms. Myxobacteria are a prolific source of novel bioactive natural ... Read more >>

ACS Chem Biol (ACS chemical biology)
[2017, 12(7):1805-1812]

Cited: 12 times

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A Practical Guide to Recombineering in Photorhabdus and Xenorhabdus.

Jia Yin, Hailong Wang, Ruijuan Li, Vinothkannan Ravichandran, Xiaoying Bian, Aiying Li, Qiang Tu, A Francis Stewart, Jun Fu, Youming Zhang,

Fluent genetic manipulation of prokaryote genomes is still limited to only a few commonly used hosts. Ideally the advanced technologies available for cloning into recombinant Escherichia coli should also be applicable in other prokaryotes. In particular, 'recombineering' is mediated by the lambda Red operon that permits fluent and precise engineering ... Read more >>

Curr Top Microbiol Immunol (Current topics in microbiology and immunology)
[2017, 402:195-213]

Cited: 1 time

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RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression.

Hailong Wang, Zhen Li, Ruonan Jia, Yu Hou, Jia Yin, Xiaoying Bian, Aiying Li, Rolf Müller, A Francis Stewart, Jun Fu, Youming Zhang,

Full-length RecE and RecT from Rac prophage mediate highly efficient linear-linear homologous recombination that can be used to clone large DNA regions directly from genomic DNA into expression vectors, bypassing library construction and screening. Homologous recombination mediated by Redαβ from lambda phage has been widely used for recombinant DNA engineering. ... Read more >>

Nat Protoc (Nature protocols)
[2016, 11(7):1175-1190]

Cited: 24 times

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Two more pieces of the colibactin genotoxin puzzle from Escherichia coli show incorporation of an unusual 1-aminocyclopropanecarboxylic acid moiety.

Xiaoying Bian, Alberto Plaza, Youming Zhang, Rolf Müller,

Colibactin represents a structurally undefined class of bacterial genotoxin inducing DNA damage and genomic instability in mammalian cells, thus promoting tumour development and exacerbating lymphopenia in animal models. The colibactin biosynthetic gene cluster (<i>clb</i>) has been known for ten years and it encodes a hybrid nonribosomal peptide synthetase (NRPS)/polyketide synthase ... Read more >>

Chem Sci (Chemical science)
[2015, 6(5):3154-3160]

Cited: 22 times

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