Full Text Journal Articles by
Author Wuyang Zhu

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Rabies virus glycoprotein serology ELISA for measurement of neutralizing antibodies in sera of vaccinated human subjects.

Rongqing Zhao, Pengcheng Yu, Yi Shan, Nagarajan Thirumeni, Maohua Li, Yanli Lv, Jianli Li, Wenlin Ren, Lisong Huang, Jingshuang Wei, Yufei Sun, Wuyang Zhu, Le Sun,

BACKGROUND:Vaccination provides protection against infection by inducing VNAs mainly against RABV surface GP. The measurement of VNAs to RABV is commonly used to assess the level of immunity in humans and animals after vaccination. A VNA titer of  ≥ 0.5 IU/mL of sera indicates adequate response to vaccination. Here, we report the ... Read more >>

Vaccine (Vaccine)
[2019, 37(41):6060-6067]

Cited: 0 times

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A CpG oligodeoxynucleotide enhances the immune response to rabies vaccination in mice.

Pengcheng Yu, Jianghong Yan, Weicheng Wu, Xiaoyan Tao, Xuexin Lu, Shuqing Liu, Wuyang Zhu,

BACKGROUND:Rabies is a fatal disease that is preventable when post exposure prophylaxis (PEP) is administered in a timely fashion. CpG oligodeoxynucleotides (ODNs) can trigger cells that express Toll-like receptor 9, and their immunopotentiation activity in an inactivated aluminum-adjuvanted rabies vaccine for dogs has been identified using mouse and dog models. ... Read more >>

Virol. J. (Virology journal)
[2018, 15(1):174]

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Recombinant Mtb9.8 of Mycobacterium bovis stimulates TNF-α and IL-1β secretion by RAW264.7 macrophages through activation of NF-κB pathway via TLR2.

Shuqing Liu, Hong Jia, Shaohua Hou, Ting Xin, Xiaoyu Guo, Gaimei Zhang, Xintao Gao, Ming Li, Wuyang Zhu, Hongfei Zhu,

The Mtb9.8 antigenic protein of Mycobacterium bovis/Mycobacterium tuberculosis has been identified as a target of the T-cell response. However, the interaction of Mtb9.8 with Toll-like receptors (TLRs) and the relevant signaling pathways have not been fully clarified. In this study, recombinant Mtb9.8 (rMtb9.8) derived from M. bovis-stimulated RAW264.7 cells initiated ... Read more >>

Sci Rep (Scientific reports)
[2018, 8(1):1928]

Cited: 3 times

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Arboviruses and their related infections in China: A comprehensive field and laboratory investigation over the last 3 decades.

Guodong Liang, Xiaolong Li, Xiaoyan Gao, Shihong Fu, Huanyu Wang, Minghua Li, Zhi Lu, Wuyang Zhu, Xinjun Lu, Lihua Wang, Yuxi Cao, Ying He, Wenwen Lei,

Since the 1980s, a comprehensive field and laboratory investigation has been conducted throughout China, and a total of 29 virus species belonging to 7 families and 13 genera were identified through virological, morphological, and immunological methods, as well as whole-genome sequencing and molecular genetic analyses. Most of the virus isolates ... Read more >>

Rev. Med. Virol. (Reviews in medical virology)
[2018, 28(1):]

Cited: 2 times

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Role of systemic injection of rabies immunoglobulin in rabies vaccination.

Weichen Wu, Shuqing Liu, Pengcheng Yu, Xiaoyan Tao, Xuexin Lu, Jianghong Yan, Qian Wang, Zongshen Zhang, Wuyang Zhu,

To determine the role of systemic injection of rabies immunoglobulin (RIG) in rabies vaccination, we analyzed the level of antibody against rabies virus in the serum of mice that received various doses of RIG combined with rabies vaccine. Our results indicate that systemic injection of RIG does not contribute detectably ... Read more >>

Arch. Virol. (Archives of virology)
[2017, 162(6):1701-1703]

Cited: 1 time

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[Preparation and Preliminary Application of a Virus Library for the Neutralizing Activity of a Monoclonal Antibody Against the Rabies Virus].

Pengcheng Yu, Xiaoyan Tao, Lihua Wang, Qing Tang, Shuqing Liu, Wuyang Zhu,

To establish a method for measurement of the neutralizing activity of a monoclonal antibody against the rabies virus. Twenty-four rabies street virus-positive samples were isolated by the mouse inoculation test. Isolated rabies street viruses were cultured and the virus titer tested in N2A cells. We established a rabies street virus ... Read more >>

Bing Du Xue Bao (Bing du xue bao = Chinese journal of virology)
[2016, 32(6):790-795]

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[Advances in Application of Rabies Immunoglobulin].

Weichen Wu, Jingxia Luo, Weixian Liang, Jianghong Yan, Wuyang Zhu, Xinjun Lv,

China has the second highest number of rabies cases worldwide. There are millions of category III rabies exposure cases in China every year, which are treated with rabies immunoglobulin(RIG)and the rabies vaccine. The price of RIG is relatively expensive, and the application of RIG is relatively complicated. The rate of ... Read more >>

Bing Du Xue Bao (Bing du xue bao = Chinese journal of virology)
[2016, 32(5):666-670]

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Isolation and Genetic Characterization of Mangshi Virus: A Newly Discovered Seadornavirus of the Reoviridae Family Found in Yunnan Province, China.

Jinglin Wang, Huachun Li, Yuwen He, Yang Zhou, Jingxing Meng, Wuyang Zhu, Hongyu Chen, Defang Liao, Yunping Man,

Seadornavirus is a genus of viruses in the family Reoviridae, which consists of Banna virus, Kadipiro virus, and Liao ning virus. Banna virus is considered a potential pathogen for zoonotic diseases. Here, we describe a newly discovered Seadornavirus isolated from mosquitos (Culex tritaeniorhynchus) in Yunnan Province, China, which is related ... Read more >>

PLoS ONE (PloS one)
[2015, 10(12):e0143601]

Cited: 10 times

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[Development and Application of An Assay for High-throughput Antiviral Compounds Screening against Alphaviruses].

Yan Wei, Jianjiao Li, Huanqin Wang, Shan Cen, Guodong Liang, Wenjie Tan, Wuyang Zhu,

To establish a cell-based rapid luciferase suppression assay for high-throughput screening (HTS) anti-alphaviruses compounds screening, which could cause viral encephalitis, raise the social issues associated directly with public health and huge economic burden to the society. The Gaussia luciferase assay system was used for HTS model for identifying inhibitors of ... Read more >>

Bing Du Xue Bao (Bing du xue bao = Chinese journal of virology)
[2015, 31(6):641-646]

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A substitution in nsP1 combined with a double substitution in E2 glycoprotein renders Sindbis-like virus XJ-160 fully neurovirulent for adult mice.

Wuyang Zhu, Lingling Deng, Yan Wei, Huanqin Wang, Jinglin Wang, Guodong Liang,

Sindbis virus (SV) can be rendered neurovirulent for adult mice by a double substitution within the E2 glycoprotein, including replacing Gln at position 55 of E2 with a His (E2-55: Gln-His) and E2-70: Glu to Lys. However, the mutant Sindbis-like virus XJ-160 with the double substitution (BR-E5570) does not show ... Read more >>

Virus Res. (Virus research)
[2015, 196:1-4]

Cited: 1 time

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Vaccine strategies for the control and prevention of Japanese encephalitis in Mainland China, 1951-2011.

Xiaoyan Gao, Xiaolong Li, Minghua Li, Shihong Fu, Huanyu Wang, Zhi Lu, Yuxi Cao, Ying He, Wuyang Zhu, Tingting Zhang, Ernest A Gould, Guodong Liang,

Japanese encephalitis (JE) is arguably one of the most serious viral encephalitis diseases worldwide. China has a long history of high prevalence of Japanese encephalitis, with thousands of cases reported annually and incidence rates often exceeding 15/100,000. In global terms, the scale of outbreaks and high incidence of these pandemics ... Read more >>

PLoS Negl Trop Dis (PLoS neglected tropical diseases)
[2014, 8(8):e3015]

Cited: 23 times

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Phenotypic and molecular characteristics of plaque-purified MX10 virus, an Oriental-Australian genotype of Sindbis virus from Yunnan, China.

Wang Jinglin, Zhu Wuyang, Shihong Fu, Li Wenjuan, Zhang Hailin, Guodong Liang,

A previous investigation showed that MX10 virus, recently isolated in China, belongs to the Oriental-Australian (O/A) genotype of Sindbis virus (SINV) (Wang Jinglin, 2011, ATMH). Similar to the MRE16 isolate, the prototype O/A genotype of SINV, two derivate viruses with obviously different plaque morphologies were derived from MX10 virus, which ... Read more >>

Arch. Virol. (Archives of virology)
[2013, 158(1):71-75]

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Correction: Rapid, Specific Detection of Alphaviruses from Tissue Cultures Using a Replicon-Defective Reporter Gene Assay.

Jiangjiao Li, Wuyang Zhu, Huanqin Wang, Jiandong Li, Quanfu Zhang, Ying He, Jia Li, Juanjuan Fu, Dexin Li, Guodong Liang,

PLoS ONE (PloS one)
[2012, 7(4):]

Cited: 0 times

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Development of EGFP/GLUC-tagged Sindbis-like virus XJ-160.

Zhu Wuyang, Jiangjiao Li, Huanqin Wang, Ying He, Guodong Liang,

Based on an infectious clone of Sindbis-like virus XJ-160, recombinant vectors containing a reporter gene (enhanced green fluorescence protein [EGFP] or Gaussia luciferase [GLUC]) were constructed by placing the reporter gene cassette containing the subgenomic promoter behind the 3' terminus of the viral structural protein gene. EGFP/GLUC-tagged Sindbis-like viruses were ... Read more >>

J. Virol. Methods (Journal of virological methods)
[2013, 189(1):235-237]

Cited: 1 time

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How does cellular heparan sulfate function in viral pathogenicity?

Wuyang Zhu, Jiangjiao Li, Guodong Liang,

Heparan sulfate (HS) is ubiquitously expressed on the surfaces and in the extracellular matrix of virtually all cell types, making it an ideal receptor for viral infection. Compared with wild-type viruses, cell culture-adapted laboratory strains exhibit more efficient binding to cellular HS receptors. HS-binding viruses are typically cleared faster from ... Read more >>

Biomed. Environ. Sci. (Biomedical and environmental sciences : BES)
[2011, 24(1):81-87]

Cited: 22 times

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