Full Text Journal Articles by
Author Timothy L Mosbruger

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Genomic characterization of MICA gene using multiple next generation sequencing platforms: A validation study.

Yizhou Zou, Jamie L Duke, Deborah Ferriola, Qizhi Luo, Jenna Wasserman, Timothy L Mosbruger, Weiguang Luo, Liang Cai, Kevin Zou, Nikolaos Tairis, Georgios Damianos, Ioanna Pagkrati, Debra Kukuruga, Yanping Huang, Dimitri S Monos,

We have developed a protocol regarding the genomic characterization of the MICA gene by next generation sequencing (NGS). The amplicon includes the full length of the gene and is about 13 kb. A total of 156 samples were included in the study. Ninety-seven of these samples were previously characterized at MICA ... Read more >>

HLA (HLA)
[2020, 96(4):430-444]

Cited: 0 times

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Utilizing nanopore sequencing technology for the rapid and comprehensive characterization of eleven HLA loci; addressing the need for deceased donor expedited HLA typing.

Timothy L Mosbruger, Amalia Dinou, Jamie L Duke, Deborah Ferriola, Hilary Mehler, Ioanna Pagkrati, Georgios Damianos, Eric Mbunwe, Mahdi Sarmady, Ioannis Lyratzakis, Sarah A Tishkoff, Anh Dinh, Dimitri S Monos,

The comprehensive characterization of human leukocyte antigen (HLA) genomic sequences remains a challenging problem. Despite the significant advantages of next-generation sequencing (NGS) in the field of Immunogenetics, there has yet to be a single solution for unambiguous, accurate, simple, cost-effective, and timely genotyping necessary for all clinical applications. This report ... Read more >>

Hum Immunol (Human immunology)
[2020, 81(8):413-422]

Cited: 0 times

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Resolving MiSeq-Generated Ambiguities in HLA-DPB1 Typing by Using the Oxford Nanopore Technology.

Jamie L Duke, Timothy L Mosbruger, Deborah Ferriola, Nilesh Chitnis, Taishan Hu, Nikolaos Tairis, David J Margolis, Dimitri S Monos,

The technical limitations of current next-generation sequencing technologies, combined with an ever-increasing number of human leukocyte antigen (HLA) alleles, form the basis for the additional ambiguities encountered at an increasing rate in clinical practice. HLA-DPB1 characterization, particularly, generates a significant percentage of ambiguities (25.5%), posing a challenge for accurate and ... Read more >>

J Mol Diagn (The Journal of molecular diagnostics : JMD)
[2019, 21(5):852-861]

Cited: 1 time

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Transcriptome-wide profiling of multiple RNA modifications simultaneously at single-base resolution.

Vahid Khoddami, Archana Yerra, Timothy L Mosbruger, Aaron M Fleming, Cynthia J Burrows, Bradley R Cairns,

The breadth and importance of RNA modifications are growing rapidly as modified ribonucleotides can impact the sequence, structure, function, stability, and fate of RNAs and their interactions with other molecules. Therefore, knowing cellular RNA modifications at single-base resolution could provide important information regarding cell status and fate. A current major ... Read more >>

Proc Natl Acad Sci U S A (Proceedings of the National Academy of Sciences of the United States of America)
[2019, 116(14):6784-6789]

Cited: 13 times

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Next-generation HLA typing of 382 International Histocompatibility Working Group reference B-lymphoblastoid cell lines: Report from the 17th International HLA and Immunogenetics Workshop.

Lisa E Creary, Sandra G Guerra, Winnie Chong, Colin J Brown, Thomas R Turner, James Robinson, Will P Bultitude, Neema P Mayor, Steven G E Marsh, Katsuyuki Saito, Kevin Lam, Jamie L Duke, Timothy L Mosbruger, Deborah Ferriola, Dimitrios Monos, Amanda Willis, Medhat Askar, Gottfried Fischer, Chee Loong Saw, Jiannis Ragoussis, Martin Petrek, Carles Serra-Pagés, Manel Juan, Catherine Stavropoulos-Giokas, Amalia Dinou, Reem Ameen, Salem Al Shemmari, Eric Spierings, Ketevan Gendzekhadze, Gerald P Morris, Qiuheng Zhang, Zahra Kashi, Susan Hsu, Sridevi Gangavarapu, Kalyan C Mallempati, Fumiko Yamamoto, Kazutoyo Osoegawa, Tamara Vayntrub, Chia-Jung Chang, John A Hansen, Marcelo A Fernández-Viňa,

Extended molecular characterization of HLA genes in the IHWG reference B-lymphoblastoid cell lines (B-LCLs) was one of the major goals for the 17th International HLA and Immunogenetics Workshop (IHIW). Although reference B-LCLs have been examined extensively in previous workshops complete high-resolution typing was not completed for all the classical class ... Read more >>

Hum Immunol (Human immunology)
[2019, 80(7):449-460]

Cited: 2 times

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FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer.

Soledad A Camolotto, Shrivatsav Pattabiraman, Timothy L Mosbruger, Alex Jones, Veronika K Belova, Grace Orstad, Mitchell Streiff, Lydia Salmond, Chris Stubben, Klaus H Kaestner, Eric L Snyder,

Changes in cancer cell identity can alter malignant potential and therapeutic response. Loss of the pulmonary lineage specifier NKX2-1 augments the growth of KRAS-driven lung adenocarcinoma and causes pulmonary to gastric transdifferentiation. Here, we show that the transcription factors FoxA1 and FoxA2 are required for initiation of mucinous NKX2-1-negative lung ... Read more >>

Elife (eLife)
[2018, 7:]

Cited: 8 times

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The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment.

Gurkan Mollaoglu, Alex Jones, Sarah J Wait, Anandaroop Mukhopadhyay, Sangmin Jeong, Rahul Arya, Soledad A Camolotto, Timothy L Mosbruger, Chris J Stubben, Christopher J Conley, Arjun Bhutkar, Jeffery M Vahrenkamp, Kristofer C Berrett, Melissa H Cessna, Thomas E Lane, Benjamin L Witt, Mohamed E Salama, Jason Gertz, Kevin B Jones, Eric L Snyder, Trudy G Oliver,

The major types of non-small-cell lung cancer (NSCLC)-squamous cell carcinoma and adenocarcinoma-have distinct immune microenvironments. We developed a genetic model of squamous NSCLC on the basis of overexpression of the transcription factor Sox2, which specifies lung basal cell fate, and loss of the tumor suppressor Lkb1 (SL mice). SL tumors ... Read more >>

Immunity (Immunity)
[2018, 49(4):764-779.e9]

Cited: 17 times

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Characterization of 108 Genomic DNA Reference Materials for 11 Human Leukocyte Antigen Loci: A GeT-RM Collaborative Project.

Maria P Bettinotti, Deborah Ferriola, Jamie L Duke, Timothy L Mosbruger, Nikolaos Tairis, Lawrence Jennings, Lisa V Kalman, Dimitri Monos,

The highly polymorphic human leukocyte antigen (HLA) genes, located in the human major histocompatibility complex, encode the class I and II antigen-presenting molecules, which are centrally involved in the immune response. HLA typing is used for several clinical applications, such as transplantation, pharmacogenetics, and diagnosis of autoimmune disease. HLA typing ... Read more >>

J Mol Diagn (The Journal of molecular diagnostics : JMD)
[2018, 20(5):703-715]

Cited: 0 times

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Active BRAF-V600E is the key player in generation of a sessile serrated polyp-specific DNA methylation profile.

Somaye Dehghanizadeh, Vahid Khoddami, Timothy L Mosbruger, Sue S Hammoud, Kornelia Edes, Therese S Berry, Michelle Done, Wade S Samowitz, James A DiSario, Daniel G Luba, Randall W Burt, David A Jones,

Sessile serrated polyps (SSPs) have emerged as important precursors for a large number of sporadic colorectal cancers. They are difficult to detect during colonoscopy due to their flat shape and the excessive amounts of secreted mucin that cover the polyps. The underlying genetic and epigenetic basis for the emergence of ... Read more >>

PLoS One (PloS one)
[2018, 13(3):e0192499]

Cited: 3 times

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Reversible LSD1 inhibition with HCI-2509 induces the p53 gene expression signature and disrupts the MYCN signature in high-risk neuroblastoma cells.

Sumati Gupta, Kelly Doyle, Timothy L Mosbruger, Andrew Butterfield, Alexis Weston, Allison Ast, Mohan Kaadige, Anupam Verma, Sunil Sharma,

Lysine-Specific Demethylase 1 (LSD1) over-expression correlates with poorly differentiated neuroblastoma and predicts poor outcome despite multimodal therapy. We have studied the efficacy of reversible and specific LSD1 inhibition with HCI-2509 in neuroblastoma cell lines and particularly the effect of HCI-2509 on the transcriptomic profile in MYCN amplified NGP cells. Cell ... Read more >>

Oncotarget (Oncotarget)
[2018, 9(11):9907-9924]

Cited: 5 times

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miR-155 promotes FLT3-ITD-induced myeloproliferative disease through inhibition of the interferon response.

Jared A Wallace, Dominique A Kagele, Anna M Eiring, Carissa N Kim, Ruozhen Hu, Marah C Runtsch, Margaret Alexander, Thomas B Huffaker, Soh-Hyun Lee, Ami B Patel, Timothy L Mosbruger, Warren P Voth, Dinesh S Rao, Rodney R Miles, June L Round, Michael W Deininger, Ryan M O'Connell,

FLT3-ITD+ acute myeloid leukemia (AML) accounts for ∼25% of all AML cases and is a subtype that carries a poor prognosis. microRNA-155 (miR-155) is specifically overexpressed in FLT3-ITD+ AML compared with FLT3 wild-type (FLT3-WT) AML and is critical for the growth of FLT3-ITD+ AML cells in vitro. However, miR-155's role ... Read more >>

Blood (Blood)
[2017, 129(23):3074-3086]

Cited: 16 times

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MYC Drives Progression of Small Cell Lung Cancer to a Variant Neuroendocrine Subtype with Vulnerability to Aurora Kinase Inhibition.

Gurkan Mollaoglu, Matthew R Guthrie, Stefanie Böhm, Johannes Brägelmann, Ismail Can, Paul M Ballieu, Annika Marx, Julie George, Christine Heinen, Milind D Chalishazar, Haixia Cheng, Abbie S Ireland, Kendall E Denning, Anandaroop Mukhopadhyay, Jeffery M Vahrenkamp, Kristofer C Berrett, Timothy L Mosbruger, Jun Wang, Jessica L Kohan, Mohamed E Salama, Benjamin L Witt, Martin Peifer, Roman K Thomas, Jason Gertz, Jane E Johnson, Adi F Gazdar, Robert J Wechsler-Reya, Martin L Sos, Trudy G Oliver,

Loss of the tumor suppressors RB1 and TP53 and MYC amplification are frequent oncogenic events in small cell lung cancer (SCLC). We show that Myc expression cooperates with Rb1 and Trp53 loss in the mouse lung to promote aggressive, highly metastatic tumors, that are initially sensitive to chemotherapy followed by ... Read more >>

Cancer Cell (Cancer cell)
[2017, 31(2):270-285]

Cited: 82 times

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MEK Inhibitors Reverse Growth of Embryonal Brain Tumors Derived from Oligoneural Precursor Cells.

Katarzyna Modzelewska, Elena F Boer, Timothy L Mosbruger, Daniel Picard, Daniela Anderson, Rodney R Miles, Mitchell Kroll, William Oslund, Theodore J Pysher, Joshua D Schiffman, Randy Jensen, Cicely A Jette, Annie Huang, Rodney A Stewart,

Malignant brain tumors are the leading cause of cancer-related deaths in children. Primitive neuroectodermal tumors of the CNS (CNS-PNETs) are particularly aggressive embryonal tumors of unknown cellular origin. Recent genomic studies have classified CNS-PNETs into molecularly distinct subgroups that promise to improve diagnosis and treatment; however, the lack of cell- ... Read more >>

Cell Rep (Cell reports)
[2016, 17(5):1255-1264]

Cited: 11 times

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Genome-Wide CRISPR-Cas9 Screen Identifies MicroRNAs That Regulate Myeloid Leukemia Cell Growth.

Jared Wallace, Ruozhen Hu, Timothy L Mosbruger, Timothy J Dahlem, W Zac Stephens, Dinesh S Rao, June L Round, Ryan M O'Connell,

Mammalian microRNA expression is dysregulated in human cancer. However, the functional relevance of many microRNAs in the context of tumor biology remains unclear. Using CRISPR-Cas9 technology, we performed a global loss-of-function screen to simultaneously test the functions of individual microRNAs and protein-coding genes during the growth of a myeloid leukemia ... Read more >>

PLoS One (PloS one)
[2016, 11(4):e0153689]

Cited: 19 times

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Discordant Haplotype Sequencing Identifies Functional Variants at the 2q33 Breast Cancer Risk Locus.

Nicola J Camp, Wei-Yu Lin, Alex Bigelow, George J Burghel, Timothy L Mosbruger, Marina A Parry, Rosalie G Waller, Sushilaben H Rigas, Pei-Yi Tai, Kristofer Berrett, Venkatesh Rajamanickam, Rachel Cosby, Ian W Brock, Brandt Jones, Dan Connley, Robert Sargent, Guoying Wang, Rachel E Factor, Philip S Bernard, Lisa Cannon-Albright, Stacey Knight, Ryan Abo, Theresa L Werner, Malcolm W R Reed, Jason Gertz, Angela Cox,

The findings from genome-wide association studies hold enormous potential for novel insight into disease mechanisms. A major challenge in the field is to map these low-risk association signals to their underlying functional sequence variants (FSV). Simple sequence study designs are insufficient, as the vast numbers of statistically comparable variants and ... Read more >>

Cancer Res (Cancer research)
[2016, 76(7):1916-1925]

Cited: 3 times

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Exosome-delivered microRNAs modulate the inflammatory response to endotoxin.

Margaret Alexander, Ruozhen Hu, Marah C Runtsch, Dominique A Kagele, Timothy L Mosbruger, Tanya Tolmachova, Miguel C Seabra, June L Round, Diane M Ward, Ryan M O'Connell,

MicroRNAs regulate gene expression posttranscriptionally and function within the cells in which they are transcribed. However, recent evidence suggests that microRNAs can be transferred between cells and mediate target gene repression. We find that endogenous miR-155 and miR-146a, two critical microRNAs that regulate inflammation, are released from dendritic cells within ... Read more >>

Nat Commun (Nature communications)
[2015, 6:7321]

Cited: 223 times

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A comparison of deep sequencing of TCRG rearrangements vs traditional capillary electrophoresis for assessment of clonality in T-Cell lymphoproliferative disorders.

Jonathan A Schumacher, Eric J Duncavage, Timothy L Mosbruger, Philippe M Szankasi, Todd W Kelley,

OBJECTIVES: To design and evaluate a next-generation sequencing (NGS)-based method for T-cell receptor γ (TCRG) gene-based T-cell clonality testing on the Ion Torrent Personal Genome Machine (Life Technologies, Carlsbad, CA) platform. METHODS: We analyzed a series of peripheral blood, bone marrow, and formalin-fixed paraffin-embedded tissue specimens with NGS vs traditional ... Read more >>

Am. J. Clin. Pathol. (American journal of clinical pathology)
[2014, 141(3):348-359]

Cited: 16 times

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Large-scale candidate gene analysis of spontaneous clearance of hepatitis C virus.

Timothy L Mosbruger, Priya Duggal, James J Goedert, Gregory D Kirk, W Keith Hoots, Leslie H Tobler, Michael Busch, Marion G Peters, Hugo R Rosen, David L Thomas, Chloe L Thio,

Human genetic variation is a determinant of recovery from acute hepatitis C virus (HCV) infection; however, to date, single-nucleotide polymorphisms (SNPs) in only a limited number of genes have been studied with respect to HCV clearance. We determined whether SNPs in 112 selected immune response genes are important for HCV ... Read more >>

J Infect Dis (The Journal of infectious diseases)
[2010, 201(9):1371-1380]

Cited: 41 times

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