Full Text Journal Articles by
Author Steve F Perry

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Hif-1α is not required for the development of cardiac adrenergic control in zebrafish (Danio rerio).

William Joyce, Steve F Perry,

Adrenergic regulation, acting via the sympathetic nervous system, provides a major mechanism to control cardiac function. It has recently been shown that hypoxia inducible factor-1α (Hif-1α) is necessary for normal development of sympathetic innervation and control of cardiac function in the mouse. To investigate whether this may represent a fundamental ... Read more >>

J Exp Zool A Ecol Integr Physiol (Journal of experimental zoology. Part A, Ecological and integrative physiology)
[2021, 335(7):623-631]

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Does hypoxia-inducible factor 1α play a role in regulating cutaneous oxygen flux in larval zebrafish (Danio rerio)?

Julian J Parker, Steve F Perry,

Previous studies have demonstrated that hypoxia tolerance is improved in zebrafish (Danio rerio) larvae after prior exposure to lowered ambient O<sub>2</sub> levels. Such improved hypoxia performance was attributed in part, to increased levels of hypoxia-inducible factor 1α (Hif-1α) exerting downstream effects on various physiological processes including promotion of trunk skin ... Read more >>

J Comp Physiol B (Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology)
[2021, 191(4):645-655]

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Disruption of tph1 genes demonstrates the importance of serotonin in regulating ventilation in larval zebrafish (Danio rerio).

Yihang Kevin Pan, Greg Jensen, Steve F Perry,

Serotonergic neuroepithelial cells (NECs) in larval zebrafish are believed to be O<sub>2</sub> chemoreceptors. Serotonin (5-HT) within these NECs has been implicated as a neurotransmitter mediating the hypoxic ventilatory response (HVR). Here, we use knockout approaches to discern the role of 5-HT in regulating the HVR by targeting the rate limiting ... Read more >>

Respir Physiol Neurobiol (Respiratory physiology & neurobiology)
[2021, 285:103594]

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Use of a carbonic anhydrase Ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio).

Alex M Zimmer, Milica Mandic, Hong Meng Yew, Emma Kunert, Yihang K Pan, Jimmy Ha, Raymond W M Kwong, Kathleen M Gilmour, Steve F Perry,

In fishes, branchial cytosolic carbonic anhydrase (CA) plays an important role in ion and acid-base regulation. The Ca17a isoform in zebrafish (<i>Danio rerio</i>) is expressed abundantly in Na<sup>+</sup>-absorbing/H<sup>+</sup>-secreting H<sup>+</sup>-ATPase-rich (HR) cells. The present study aimed to identify the role of Ca17a in ion and acid-base regulation across life stages using ... Read more >>

Am J Physiol Regul Integr Comp Physiol (American journal of physiology. Regulatory, integrative and comparative physiology)
[2021, 320(1):R55-R68]

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Respiratory responses to external ammonia in zebrafish (Danio rerio).

Cosima Porteus, Yusuke Kumai, Sara J Abdallah, Hong M Yew, Raymond W M Kwong, Yihang Pan, William K Milsom, Steve F Perry,

The effects of high external ammonia (HEA) exposure on breathing and the potential involvement of ammonia transporting Rh proteins in ammonia sensing were assessed in larval and adult zebrafish. Acute exposure of adults to either 250 or 500 μM (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> caused increases in ventilation amplitude (A<sub>VENT</sub>) without affecting frequency (f<sub>VENT</sub>), resembling ... Read more >>

Comp Biochem Physiol A Mol Integr Physiol (Comparative biochemistry and physiology. Part A, Molecular & integrative physiology)
[2021, 251:110822]

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Correction: Reassessing the contribution of the Na+/H+ exchanger Nhe3b to Na+ uptake in zebrafish (Danio rerio) using CRISPR/Cas9 gene editing.

Alex M Zimmer, Khatereh Shir-Mohammadi, Raymond W M Kwong, Steve F Perry,

J Exp Biol (The Journal of experimental biology)
[2020, 223(Pt 17):]

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Respirometry and cutaneous oxygen flux measurements reveal a negligible aerobic cost of ion regulation in larval zebrafish (Danio rerio).

Julian J Parker, Alex M Zimmer, Steve F Perry,

Fishes living in fresh water counter the passive loss of salts by actively absorbing ions through specialized cells termed ionocytes. Ionocytes contain ATP-dependent transporters and are enriched with mitochondria; therefore ionic regulation is an energy-consuming process. The purpose of this study was to assess the aerobic costs of ion transport ... Read more >>

J Exp Biol (The Journal of experimental biology)
[2020, 223(Pt 18):]

Cited: 2 times

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Loss of hypoxia-inducible factor 1α affects hypoxia tolerance in larval and adult zebrafish (Danio rerio).

Milica Mandic, Carol Best, Steve F Perry,

The coordination of the hypoxic response is attributed, in part, to hypoxia-inducible factor 1α (Hif-1α), a regulator of hypoxia-induced transcription. After the teleost-specific genome duplication, most teleost fishes lost the duplicate copy of Hif-1α, except species in the cyprinid lineage that retained both paralogues of Hif-1α (Hif1aa and Hif1ab). Little ... Read more >>

Proc Biol Sci (Proceedings. Biological sciences)
[2020, 287(1927):20200798]

Cited: 1 time

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Hypoxia inducible factor-1α knockout does not impair acute thermal tolerance or heat hardening in zebrafish.

William Joyce, Steve F Perry,

The rapid increase in critical thermal maximum (CT<sub>max</sub>) in fish (or other animals) previously exposed to critically high temperature is termed 'heat hardening', which likely represents a key strategy to cope with increasingly extreme environments. The physiological mechanisms that determine acute thermal tolerance, and the underlying pathways facilitating heat hardening, ... Read more >>

Biol Lett (Biology letters)
[2020, 16(7):20200292]

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Relationships between the peak hypoxic ventilatory response and critical O2 tension in larval and adult zebrafish (Danio rerio).

Milica Mandic, Yihang K Pan, Kathleen M Gilmour, Steve F Perry,

Fish increase ventilation during hypoxia, a reflex termed the hypoxic ventilatory response (HVR). The HVR is an effective mechanism to increase O<sub>2</sub> uptake, but at a high metabolic cost. Therefore, when hypoxia becomes severe enough, ventilation declines, as its benefit is diminished. The water oxygen partial pressure (<i>P</i>w<sub>O<sub>2</sub></sub> ) at ... Read more >>

J Exp Biol (The Journal of experimental biology)
[2020, 223(Pt 7):]

Cited: 1 time

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The Rhesus glycoprotein Rhcgb is expendable for ammonia excretion and Na+ uptake in zebrafish (Danio rerio).

Alex M Zimmer, Steve F Perry,

In zebrafish (Danio rerio), the ammonia-transporting Rhesus glycoprotein Rhcgb is implicated in mechanisms of ammonia excretion and Na<sup>+</sup> uptake. In particular, Rhcgb is thought to play an important role in maintaining ammonia excretion in response to alkaline conditions and high external ammonia (HEA) exposure, in addition to facilitating Na<sup>+</sup> uptake ... Read more >>

Comp Biochem Physiol A Mol Integr Physiol (Comparative biochemistry and physiology. Part A, Molecular & integrative physiology)
[2020, 247:110722]

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Neuroendocrine control of breathing in fish.

Yihang Kevin Pan, Steve F Perry,

Beginning with the discovery more than 35 years ago that oxygen chemoreceptors of the fish gill are enriched with serotonin, numerous studies have examined the importance of this, and other neuroendocrine factors in piscine chemoreceptor function, and in particular on the chemoreceptor-mediated reflex control of breathing. However, despite these studies, ... Read more >>

Mol Cell Endocrinol (Molecular and cellular endocrinology)
[2020, 509:110800]

Cited: 2 times

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Reassessing the contribution of the Na+/H+ exchanger Nhe3b to Na+ uptake in zebrafish (Danio rerio) using CRISPR/Cas9 gene editing.

Alex M Zimmer, Khatereh Shir-Mohammadi, Raymond W M Kwong, Steve F Perry,

Freshwater fishes absorb Na<sup>+</sup> from their dilute environment using ion-transporting cells. In larval zebrafish (<i>Danio rerio</i>), Na<sup>+</sup> uptake is coordinated by (1) Na<sup>+</sup>/H<sup>+</sup> exchanger 3b (Nhe3b) and (2) H<sup>+</sup>-ATPase-powered electrogenic uptake in H<sup>+</sup>-ATPase-rich (HR) cells and by (3) Na<sup>+</sup>-Cl<sup>-</sup>-cotransporter (Ncc) expressed in NCC cells. The present study aimed to better ... Read more >>

J Exp Biol (The Journal of experimental biology)
[2020, 223(Pt 2):]

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Breathing with fins: do the pectoral fins of larval fishes play a respiratory role?

Alex M Zimmer, Milica Mandic, Kaitlyn M Rourke, Steve F Perry,

Convective water flow across respiratory epithelia in water-breathing organisms maintains transcutaneous oxygen (O<sub>2</sub>) partial pressure (Po<sub>2</sub>) gradients that drive O<sub>2</sub> uptake. Following hatch, larval fishes lack a developed gill and the skin is the dominant site of gas transfer, yet few studies have addressed the contribution of convective water flow ... Read more >>

Am J Physiol Regul Integr Comp Physiol (American journal of physiology. Regulatory, integrative and comparative physiology)
[2020, 318(1):R89-R97]

Cited: 4 times

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Use of gene knockout to examine serotonergic control of ion uptake in zebrafish reveals the importance of controlling for genetic background: A cautionary tale.

Alex M Zimmer, Jeffrey Do, Kaitlin Szederkenyi, Alex Chen, Alden L R Morgan, Greg Jensen, Yihang K Pan, Kathleen M Gilmour, Steve F Perry,

Freshwater (FW) fishes inhabit dilute environments and must actively absorb ions in order to counteract diffusive salt loss. Neuroendocrine control of ion uptake in FW fishes is an important feature of ion homeostasis and several important neuroendocrine factors have been identified. The role of serotonin (5-HT), however, has received less ... Read more >>

Comp Biochem Physiol A Mol Integr Physiol (Comparative biochemistry and physiology. Part A, Molecular & integrative physiology)
[2019, 238:110558]

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Evaluating the physiological significance of hypoxic hyperventilation in larval zebrafish (Danio rerio).

Yihang K Pan, Milica Mandic, Alex M Zimmer, Steve F Perry,

In water-breathing fishes, the hypoxic ventilatory response (HVR) represents an increase in water flow over the gills during exposure to lowered ambient O<sub>2</sub> levels. The HVR is a critical defense mechanism that serves to delay the negative consequences of hypoxia on aerobic respiration. However, the physiological significance of the HVR ... Read more >>

J Exp Biol (The Journal of experimental biology)
[2019, 222(Pt 13):]

Cited: 3 times

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Loss-of-function approaches in comparative physiology: is there a future for knockdown experiments in the era of genome editing?

Alex M Zimmer, Yihang K Pan, Theanuga Chandrapalan, Raymond W M Kwong, Steve F Perry,

Loss-of-function technologies, such as morpholino- and RNAi-mediated gene knockdown, and TALEN- and CRISPR/Cas9-mediated gene knockout, are widely used to investigate gene function and its physiological significance. Here, we provide a general overview of the various knockdown and knockout technologies commonly used in comparative physiology and discuss the merits and drawbacks ... Read more >>

J Exp Biol (The Journal of experimental biology)
[2019, 222(Pt 7):]

Cited: 9 times

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Hif-1α paralogs play a role in the hypoxic ventilatory response of larval and adult zebrafish (Danio rerio).

Milica Mandic, Velislava Tzaneva, Vincent Careau, Steve F Perry,

Hypoxia-inducible factor (Hif) 1α, an extensively studied transcription factor, is involved in the regulation of many biological processes in hypoxia including the hypoxic ventilatory response. In zebrafish, there are two paralogs of Hif-1α (Hif-1A and Hif-1B), but little is known about the specific roles or potential sub-functionalization of the paralogs ... Read more >>

J Exp Biol (The Journal of experimental biology)
[2019, 222(Pt 2):]

Cited: 4 times

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Conflict and Compromise: Using Reversible Remodeling to Manage Competing Physiological Demands at the Fish Gill.

Kathleen M Gilmour, Steve F Perry,

The structural features of the fish gill necessary for oxygen uptake also favor undesirable, passive movements of ions and water. Reversible gill remodeling is one solution to this conflict. Cell masses that limit functional surface area are lost when oxygen availability decreases in hypoxia or oxygen demand increases with exercise ... Read more >>

Physiology (Bethesda) (Physiology (Bethesda, Md.))
[2018, 33(6):412-422]

Cited: 2 times

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Assessing the role of the acid-sensing ion channel ASIC4b in sodium uptake by larval zebrafish.

Alex M Zimmer, Agnieszka K Dymowska, Yusuke Kumai, Greg G Goss, Steve F Perry, Raymond W M Kwong,

Na<sup>+</sup> uptake in larval zebrafish (Danio rerio) is coordinated by three mechanisms: Na<sup>+</sup>/H<sup>+</sup>-exchanger 3b (NHE3b) expressed in H<sup>+</sup>-ATPase-rich (HR) cells, an unidentified Na<sup>+</sup> channel coupled to electrogenic H<sup>+</sup>-ATPase expressed in HR cells, and Na<sup>+</sup>-Cl<sup>-</sup>-cotransporter (NCC) expressed in NCC cells. Recently, acid-sensing ion channels (ASICs) were proposed to be the putative ... Read more >>

Comp Biochem Physiol A Mol Integr Physiol (Comparative biochemistry and physiology. Part A, Molecular & integrative physiology)
[2018, 226:1-10]

Cited: 0 times

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Air breathing and aquatic gas exchange during hypoxia in armoured catfish.

Graham R Scott, Victoria Matey, Julie-Anne Mendoza, Kathleen M Gilmour, Steve F Perry, Vera M F Almeida-Val, Adalberto L Val,

Air breathing in fish is commonly believed to have arisen as an adaptation to aquatic hypoxia. The effectiveness of air breathing for tissue O<sub>2</sub> supply depends on the ability to avoid O<sub>2</sub> loss as oxygenated blood from the air-breathing organ passes through the gills. Here, we evaluated whether the armoured ... Read more >>

J Comp Physiol B (Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology)
[2017, 187(1):117-133]

Cited: 6 times

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A role for sodium-chloride cotransporters in the rapid regulation of ion uptake following acute environmental acidosis: new insights from the zebrafish model.

Raymond W M Kwong, Steve F Perry,

The effects of acute exposure to acidic water on Na<sup>+</sup> and Cl<sup>-</sup> homeostasis, and the mechanisms underlying their compensatory regulation, were investigated in the larval zebrafish Danio rerio Exposure to acidic water (pH 4.0; control pH 7.6) for 2 h significantly reduced Na<sup>+</sup> uptake and whole body Na<sup>+</sup> content. Nevertheless, ... Read more >>

Am J Physiol Cell Physiol (American journal of physiology. Cell physiology)
[2016, 311(6):C931-C941]

Cited: 3 times

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Inhibition of calcium uptake during hypoxia in developing zebrafish is mediated by hypoxia-inducible factor.

Raymond W M Kwong, Yusuke Kumai, Velislava Tzaneva, Estelle Azzi, Nina Hochhold, Cayleih Robertson, Bernd Pelster, Steve F Perry,

The present study investigated the potential role of hypoxia-inducible factor (HIF) in calcium homeostasis in developing zebrafish (Danio rerio). It was demonstrated that zebrafish raised in hypoxic water (30 mmHg; control, 155 mmHg P<sub>O<sub>2</sub></sub> ) until 4 days post-fertilization exhibited a substantial reduction in whole-body Ca<sup>2+</sup> levels and Ca<sup>2+</sup> uptake. Ca<sup>2+</sup> uptake in ... Read more >>

J Exp Biol (The Journal of experimental biology)
[2016, 219(Pt 24):3988-3995]

Cited: 1 time

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Role of endogenous carbon monoxide in the control of breathing in zebrafish (Danio rerio).

Velislava Tzaneva, Steve F Perry,

Carbon monoxide (CO) is a gaseous signaling molecule and is produced in vivo from the intracellular breakdown of heme via the heme oxygenase (HO) family of enzymes. In this study we investigated the role of the HO-1/CO system in the control of ventilation in zebrafish, Danio rerio Immunohistochemistry revealed the ... Read more >>

Am J Physiol Regul Integr Comp Physiol (American journal of physiology. Regulatory, integrative and comparative physiology)
[2016, 311(6):R1262-R1270]

Cited: 4 times

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Neuroendocrine control of ionic balance in zebrafish.

Raymond W M Kwong, Yusuke Kumai, Steve F Perry,

Zebrafish (Danio rerio) is an emerging model for integrative physiological research. In this mini-review, we discuss recent advances in the neuroendocrine control of ionic balance in this species, and identify current knowledge gaps and issues that would benefit from further investigation. Zebrafish inhabit a hypo-ionic environment and therefore are challenged ... Read more >>

Gen Comp Endocrinol (General and comparative endocrinology)
[2016, 234:40-46]

Cited: 6 times

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