Full Text Journal Articles by
Author Sergio Giuffrida

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Closed-Locked and Apo-Resting State Structures of the Human α7 Nicotinic Receptor: A Computational Study.

Letizia Chiodo, Thérèse E Malliavin, Sergio Giuffrida, Luca Maragliano, Grazia Cottone,

Nicotinic acetylcholine receptors, belonging to the Cys-loop superfamily of ligand-gated ion channels (LGICs), are membrane proteins present in neurons and at neuromuscular junctions. They are responsible for signal transmission, and their function is regulated by neurotransmitters, agonists, and antagonists drugs. A detailed knowledge of their conformational transition in response to ... Read more >>

J Chem Inf Model (Journal of chemical information and modeling)
[2018, 58(11):2278-2293]

Cited: 1 time

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Bioprotection Can Be Tuned with a Proper Protein/Saccharide Ratio: The Case of Solid Amorphous Matrices.

Sergio Giuffrida, Lorenzo Cordone, Grazia Cottone,

Saccharides, and in particular trehalose, are well known for their high efficiency in protecting biostructures against adverse environmental conditions. The protein dynamics is known to be highly inhibited in a low-water trehalose host medium, the inhibition being markedly dependent on the amount of residual water. Besides hydration, the protein/sugar ratio ... Read more >>

J Phys Chem B (The journal of physical chemistry. B)
[2018, 122(37):8642-8653]

Cited: 1 time

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Biopreservation of Myoglobin in Crowded Environment: A Comparison between Gelatin and Trehalose Matrixes.

Enrico F Semeraro, Sergio Giuffrida, Grazia Cottone, Antonio Cupane,

Biopreservation by sugar and/or polymeric matrixes is a thoroughly studied research topic with wide technological relevance. Ternary amorphous systems containing both saccharides and proteins are extensively exploited to model the in vivo biopreservation process. With the aim of disentangling the effect of saccharides and polypeptidic crowders (such as gelatin) on ... Read more >>

J Phys Chem B (The journal of physical chemistry. B)
[2017, 121(37):8731-8741]

Cited: 1 time

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The water association band as a marker of hydrogen bonds in trehalose amorphous matrices.

Sergio Giuffrida, Grazia Cottone, Lorenzo Cordone,

The relevant role played by residual water in modulating the dynamics and structure of a protein, a matrix and their coupling has been thoroughly studied in bioprotective amorphous saccharide matrices via experiments and simulations. In order to better characterize this residual water and the hydrogen bond structures in which it ... Read more >>

Phys Chem Chem Phys (Physical chemistry chemical physics : PCCP)
[2017, 19(6):4251-4265]

Cited: 0 times

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Protein thermal denaturation and matrix glass transition in different protein-trehalose-water systems.

Giuseppe Bellavia, Sergio Giuffrida, Grazia Cottone, Antonio Cupane, Lorenzo Cordone,

Biopreservation by saccharides is a widely studied issue due to its scientific and technological importance; in particular, ternary amorphous protein-saccharide-water systems are extensively exploited to model the characteristics of the in vivo biopreservation process. We present here a differential scanning calorimetry (DSC) study on amorphous trehalose-water systems with embedded different ... Read more >>

J Phys Chem B (The journal of physical chemistry. B)
[2011, 115(19):6340-6346]

Cited: 16 times

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Myoglobin embedded in saccharide amorphous matrices: water-dependent domains evidenced by small angle X-ray scattering.

Alessandro Longo, Sergio Giuffrida, Grazia Cottone, Lorenzo Cordone,

We report Small Angle X-ray Scattering (SAXS) measurements performed on samples of carboxy-myoglobin (MbCO) embedded in low-water trehalose glasses. Results showed that, in such samples, "low-protein" trehalose-water domains are present, surrounded by a protein-trehalose-water background; such finding is supported by Infrared Spectroscopy (FTIR) measurements. These domains, which do not appear ... Read more >>

Phys Chem Chem Phys (Physical chemistry chemical physics : PCCP)
[2010, 12(25):6852-6858]

Cited: 5 times

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Thermal denaturation of myoglobin in water--disaccharide matrixes: relation with the glass transition of the system.

Giuseppe Bellavia, Grazia Cottone, Sergio Giuffrida, Antonio Cupane, Lorenzo Cordone,

Proteins embedded in glassy saccharide systems are protected against adverse environmental conditions [Crowe et al. Annu. Rev. Physiol. 1998, 60, 73-103]. To further characterize this process, we studied the relationship between the glass transition temperature of the protein-containing saccharide system (T(g)) and the temperature of thermal denaturation of the embedded ... Read more >>

J Phys Chem B (The journal of physical chemistry. B)
[2009, 113(33):11543-11549]

Cited: 11 times

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Adsorbed CO on group 10 metal fragments: a DFT study.

Sergio Giuffrida, Giampaolo Barone, Dario Duca,

DFT calculations on the helicopter and cartwheel rotations of one CO molecule adsorbed at the bridge site on metal-surface fragments, characterized by two (M(8)) or three (M(14)) metal-atom layers (M = Ni, Pd, Pt) were performed by the B3LYP[LANL2DZ+6-31 g(d,p)] method, to rationalize the adsorption energetics and the steric hindrance ... Read more >>

J Chem Inf Model (Journal of chemical information and modeling)
[2009, 49(5):1223-1233]

Cited: 0 times

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Role of solvent on protein-matrix coupling in MbCO embedded in water-saccharide systems: a Fourier transform infrared spectroscopy study.

Sergio Giuffrida, Grazia Cottone, Lorenzo Cordone,

Embedding protein in sugar systems of low water content enables one to investigate the protein dynamic-structure function in matrixes whose rigidity is modulated by varying the content of residual water. Accordingly, studying the dynamics and structure thermal evolution of a protein in sugar systems of different hydration constitutes a tool ... Read more >>

Biophys. J. (Biophysical journal)
[2006, 91(3):968-980]

Cited: 18 times

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Internal dynamics and protein-matrix coupling in trehalose-coated proteins.

Lorenzo Cordone, Grazia Cottone, Sergio Giuffrida, Gerardo Palazzo, Giovanni Venturoli, Cristiano Viappiani,

We review recent studies on the role played by non-liquid, water-containing matrices on the dynamics and structure of embedded proteins. Two proteins were studied, in water-trehalose matrices: a water-soluble protein (carboxy derivative of horse heart myoglobin) and a membrane protein (reaction centre from Rhodobacter sphaeroides). Several experimental techniques were used: ... Read more >>

Biochim. Biophys. Acta (Biochimica et biophysica acta)
[2005, 1749(2):252-281]

Cited: 38 times

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Light-induced protein-matrix uncoupling and protein relaxation in dry samples of trehalose-coated MbCO at room temperature.

Stefania Abbruzzetti, Sergio Giuffrida, Silvia Sottini, Cristiano Viappiani, Lorenzo Cordone,

In humid samples of trehalose-coated carboxy-myoglobin (MbCO), thermally driven conformational relaxation takes place after photodissociation of the carbon monoxide (CO) molecule at room temperature. In such samples, because of the extreme viscosity of the external matrix, photodissociated CO cannot diffuse out of the protein and explores the whole (proximal and ... Read more >>

Cell Biochem. Biophys. (Cell biochemistry and biophysics)
[2005, 43(3):431-437]

Cited: 6 times

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