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Author Seleipiri Charles

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Single-molecule FISH in C. elegans embryos reveals early embryonic expression dynamics of par-2 , lgl-1 and chin-1 and possible differences between hyper-diverged strains.

Samiksha Kaul, Han Ting Chou, Seleipiri Charles, Guillaume Aubry, Hang Lu, Annalise B Paaby,

Wild <i>C. elegans</i> strains harbor natural variation in developmental pathways, but investigating these differences requires precise and well-powered phenotyping methods. Here we employ a microfluidics platform for single-molecule FISH to simultaneously visualize the transcripts of three genes in embryos of two distinct strains. We capture transcripts at high resolution by ... Read more >>

MicroPubl Biol (microPublication biology)
[2022, 2022:]

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High-Temporal-Resolution smFISH Method for Gene Expression Studies in Caenorhabditis elegans Embryos.

Seleipiri Charles, Guillaume Aubry, Han-Ting Chou, Annalise B Paaby, Hang Lu,

Recent development in fluorescence-based molecular tools has contributed significantly to developmental studies, including embryogenesis. Many of these tools rely on multiple steps of sample manipulation, so obtaining large sample sizes presents a major challenge as it can be labor-intensive and time-consuming. However, large sample sizes are required to uncover critical ... Read more >>

Anal Chem (Analytical chemistry)
[2021, 93(3):1369-1376]

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