Full Text Journal Articles by
Author Scott K. Dessain


Find full text journal articles

Monoclonal Antibodies From Anti-NMDA Receptor Encephalitis Patient as a Tool to Study Autoimmune Seizures.

Olga Taraschenko, Howard S Fox, Ember Eldridge, Wenyi Wang, Samuel W Dowd, Fetweh Al-Saleem, Chandana Devi Kattala, Scott K Dessain, Raymond Dingledine,

Anti-N-methyl-D-aspartate (NMDA) receptor encephalitis manifests with precipitous cognitive decline, abnormal movements, and severe seizures that can be challenging to control with conventional anti-seizure medications. We previously demonstrated that intracerebroventricular (i.c.v.) administration of cerebrospinal fluid from affected patients, or purified NMDA receptor antibodies from encephalitis patients to mice precipitated seizures, thereby ... Read more >>

Front Neurosci (Frontiers in neuroscience)
[2021, 15:710650]

Cited: 0 times

View full text PDF listing >>

Human IgA Monoclonal Antibodies That Neutralize Poliovirus, Produced by Hybridomas and Recombinant Expression.

Rama Devudu Puligedda, Vladimir Vigdorovich, Diana Kouiavskaia, Chandana Devi Kattala, Jiang-Yang Zhao, Fetweh H Al-Saleem, Konstantin Chumakov, D Noah Sather, Scott K Dessain,

Poliovirus (PV)-specific intestinal IgAs are important for cessation of PV shedding in the gastrointestinal tract following an acute infection with wild type or vaccine-derived PV strains. We sought to produce IgA monoclonal antibodies (mAbs) with PV neutralizing activity. We first performed de novo IgA discovery from primary human B cells ... Read more >>

Antibodies (Basel) (Antibodies (Basel, Switzerland))
[2020, 9(1):]

Cited: 2 times

View full text PDF listing >>


Capture and display of antibodies secreted by hybridoma cells enables fluorescent on-cell screening.

Rama Devudu Puligedda, Rashmi Sharma, Fetweh H Al-Saleem, Diana Kouiavskaia, Arul Balaji Velu, Chandana Devi Kattala, George C Prendergast, David R Lynch, Konstantin Chumakov, Scott K Dessain,

Hybridoma methods for monoclonal antibody (mAb) cloning are a mainstay of biomedical research, but they are hindered by the need to maintain hybridomas in oligoclonal pools during antibody screening. Here, we describe a system in which hybridomas specifically capture and display the mAbs they secrete: On-Cell mAb Screening (OCMS™). In ... Read more >>

MAbs (mAbs)
[2019, 11(3):546-558]

Cited: 3 times

View full text PDF listing >>

Monoclonal antibodies from a patient with anti-NMDA receptor encephalitis.

Rashmi Sharma, Fetweh H Al-Saleem, Jessica Panzer, Jiwon Lee, Rama Devudu Puligedda, Liza F Felicori, Chandana Devi Kattala, Amy J Rattelle, Gregory Ippolito, Robert H Cox, David R Lynch, Scott K Dessain,

<h4>Objective</h4>Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal encephalitis attributed to autoantibodies against the <i>N</i>-methyl-D-aspartate receptor (NMDAR). We sought to clone and characterize monoclonal antibodies (mAbs) from an ANRE patient.<h4>Methods</h4>We used a hybridoma method to clone two IgG mAbs from a female patient with ANRE without teratoma, and characterized their ... Read more >>

Ann Clin Transl Neurol (Annals of clinical and translational neurology)
[2018, 5(8):935-951]

Cited: 8 times

View full text PDF listing >>

Membrane-bound and soluble forms of an NMDA receptor extracellular domain retain epitopes targeted in auto-immune encephalitis.

Rashmi Sharma, Fetweh H Al-Saleem, Rama Devudu Puligedda, Amy Rattelle, David R Lynch, Scott K Dessain,

<h4>Background</h4>Anti-NMDA receptor encephalitis (ANRE) is a potentially lethal disease attributed to auto-antibodies against the N-methyl-D-aspartate receptor (NMDAR). Full recovery is possible if therapy is initiated early in the disease course. Detection of ANRE antibodies in the cerebrospinal fluid (CSF) is essential for diagnosis. The assays for ANRE-associated IgGs often rely on cells ... Read more >>

BMC Biotechnol (BMC biotechnology)
[2018, 18(1):41]

Cited: 2 times

View full text PDF listing >>

RBC Adherence of Immune Complexes Containing Botulinum Toxin Improves Neutralization and Macrophage Uptake.

Fetweh H Al-Saleem, Rashmi Sharma, Rama Devudu Puligedda, Md Elias, Chandana Devi Kattala, Paul M Simon, Lance L Simpson, Scott K Dessain,

In the paralytic disease botulism, the botulinum neurotoxin (BoNT) passes through the bloodstream to reach and inactivate neuromuscular junctions. Monoclonal antibodies (mAbs) may be useful BoNT countermeasures, as mAb combinations can rapidly clear BoNT from the blood circulation. We have previously shown that the BoNT-neutralizing potency of mAbs can be ... Read more >>

Toxins (Basel) (Toxins)
[2017, 9(5):]

Cited: 3 times

View full text PDF listing >>

A human monoclonal IgG that binds aβ assemblies and diverse amyloids exhibits anti-amyloid activities in vitro and in vivo.

Yona Levites, Brian O'Nuallain, Rama Devudu Puligedda, Tomas Ondrejcak, Sharad P Adekar, Cindy Chen, Pedro E Cruz, Awilda M Rosario, Sallie Macy, Alexandra J Mably, Dominic M Walsh, Ruben Vidal, Alan Solomon, Daniel Brown, Michael J Rowan, Todd E Golde, Scott K Dessain,

Alzheimer's disease (AD) and familial Danish dementia (FDD) are degenerative neurological diseases characterized by amyloid pathology. Normal human sera contain IgG antibodies that specifically bind diverse preamyloid and amyloid proteins and have shown therapeutic potential in vitro and in vivo. We cloned one of these antibodies, 3H3, from memory B ... Read more >>

J Neurosci (The Journal of neuroscience : the official journal of the Society for Neuroscience)
[2015, 35(16):6265-6276]

Cited: 7 times

View full text PDF listing >>

Mechanisms of enhanced neutralization of botulinum neurotoxin by monoclonal antibodies conjugated to antibodies specific for the erythrocyte complement receptor.

Rashmi Sharma, Huiwu Zhao, Fetweh H Al-Saleem, Ahmed Syed Ubaid, Rama Devudu Puligedda, Andrew T Segan, Margaret A Lindorfer, Rodney Bermudez, Md Elias, Sharad P Adekar, Lance L Simpson, Ronald P Taylor, Scott K Dessain,

Immune complexes formed between monoclonal antibodies (mAbs) and toxins can neutralize toxicity in vivo by multiple mechanisms. Toxin sequestration and clearance by mAbs may be improved by enhancing their ability to bind to red blood cells (RBCs) through immune adherence. This can be achieved by converting the mAbs to heteropolymers ... Read more >>

Mol Immunol (Molecular immunology)
[2014, 57(2):247-254]

Cited: 10 times

View full text PDF listing >>

Enhanced neutralization potency of botulinum neurotoxin antibodies using a red blood cell-targeting fusion protein.

Sharad P Adekar, Andrew T Segan, Cindy Chen, Rodney Bermudez, M D Elias, Bernard H Selling, B P Kapadnis, Lance L Simpson, Paul M Simon, Scott K Dessain,

Botulinum neurotoxin (BoNT) potently inhibits cholinergic signaling at the neuromuscular junction. The ideal countermeasures for BoNT exposure are monoclonal antibodies or BoNT antisera, which form BoNT-containing immune complexes that are rapidly cleared from the general circulation. Clearance of opsonized toxins may involve complement receptor-mediated immunoadherence to red blood cells (RBC) ... Read more >>

PLoS One (PloS one)
[2011, 6(3):e17491]

Cited: 8 times

View full text PDF listing >>

Inherent anti-amyloidogenic activity of human immunoglobulin gamma heavy chains.

Sharad P Adekar, Igor Klyubin, Sally Macy, Michael J Rowan, Alan Solomon, Scott K Dessain, Brian O'Nuallain,

We have previously shown that a subpopulation of naturally occurring human IgGs were cross-reactive against conformational epitopes on pathologic aggregates of Abeta, a peptide that forms amyloid fibrils in the brains of patients with Alzheimer disease, inhibited amyloid fibril growth, and dissociated amyloid in vivo. Here, we describe similar anti-amyloidogenic ... Read more >>

J Biol Chem (The Journal of biological chemistry)
[2010, 285(2):1066-1074]

Cited: 12 times

View full text PDF listing >>

Neutralization of botulinum neurotoxin by a human monoclonal antibody specific for the catalytic light chain.

Sharad P Adekar, Tsuyoshi Takahashi, R Mark Jones, Fetweh H Al-Saleem, Denise M Ancharski, Michael J Root, B P Kapadnis, Lance L Simpson, Scott K Dessain,

<h4>Background</h4>Botulinum neurotoxins (BoNT) are a family of category A select bioterror agents and the most potent biological toxins known. Cloned antibody therapeutics hold considerable promise as BoNT therapeutics, but the therapeutic utility of antibodies that bind the BoNT light chain domain (LC), a metalloprotease that functions in the cytosol of ... Read more >>

PLoS One (PloS one)
[2008, 3(8):e3023]

Cited: 41 times

View full text PDF listing >>

Enumeration of the Simian Virus 40 Early Region Elements Necessary for Human Cell Transformation

William C. Hahn, Scott K. Dessain, Mary W. Brooks, Jessie E. King, Brian Elenbaas, David M. Sabatini, James A. DeCaprio, Robert A. Weinberg,

Mol Cell Biol (Molecular and cellular biology)
[2002, 22(10):3562-3562]

Cited: 0 times

View full text PDF listing >>



0.8022 s