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Author Scot P Ouellette

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Proximity Labeling to Map Host-Pathogen Interactions at the Membrane of a Bacteria Containing Vacuole in Chlamydia trachomatis Infected Human Cells.

Macy G Olson, Ray E Widner, Lisa M Jorgenson, Alyssa Lawrence, Dragana Lagundzin, Nicholas T Woods, Scot P Ouellette, Elizabeth A Rucks,

Many intracellular bacteria, including the obligate intracellular pathogen Chlamydia trachomatis, grow within a membrane-bound "bacteria containing vacuole" (BCV). Secreted cytosolic effectors modulate host activity, but an understanding of the host-pathogen interactions that occur at the BCV membrane is limited by the difficulty in purifying membrane fractions from infected host cells. ... Read more >>

Infect. Immun. (Infection and immunity)
[2019, :]

Cited: 0 times

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Bacterial Endosymbionts Are Common Among, but not Necessarily Within, Insect Species.

Eric J Sazama, Scot P Ouellette, Jeff S Wesner,

Bacterial endosymbionts, particularly Wolbachia (Rickettsiales: Rickettsiaceae), Rickettsia (Rickettsiales: Rickettsiaceae), and Cardinium (Bacteroidales: Bacteroidaceae), are commonly found in several arthropod groups, including insects. Most estimates of the global infection rate of Wolbachia (52% [95% credible intervals: 44-60]) show that these bacteria infect more than half of all insect species. Other endosymbionts, ... Read more >>

Environ. Entomol. (Environmental entomology)
[2019, 48(1):127-133]

Cited: 0 times

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Initial Characterization of the Two ClpP Paralogs of Chlamydia trachomatis Suggests Unique Functionality for Each.

Nicholas A Wood, Krystal Y Chung, Amanda M Blocker, Nathalia Rodrigues de Almeida, Martin Conda-Sheridan, Derek J Fisher, Scot P Ouellette,

Members of Chlamydia are obligate intracellular bacteria that differentiate between two distinct functional and morphological forms during their developmental cycle, elementary bodies (EBs) and reticulate bodies (RBs). EBs are nondividing small electron-dense forms that infect host cells. RBs are larger noninfectious replicative forms that develop within a membrane-bound vesicle, termed ... Read more >>

J. Bacteriol. (Journal of bacteriology)
[2019, 201(2):]

Cited: 0 times

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Characterization of Chlamydial Rho and the Role of Rho-Mediated Transcriptional Polarity during Interferon Gamma-Mediated Tryptophan Limitation.

Scot P Ouellette, Parker R Messerli, Nicholas A Wood, Heather Hajovsky,

As an obligate intracellular, developmentally regulated bacterium, Chlamydia is sensitive to amino acid fluctuations within its host cell. When human epithelial cells are treated with the cytokine interferon gamma (IFN-γ), the tryptophan (Trp)-degrading enzyme, indoleamine-2,3-dioxygenase, is induced. Chlamydiae within such cells are starved for Trp and enter a state of ... Read more >>

Infect. Immun. (Infection and immunity)
[2018, 86(7):]

Cited: 2 times

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Feasibility of a Conditional Knockout System for Chlamydia Based on CRISPR Interference.

Scot P Ouellette,

Chlamydia is an obligate intracellular bacterium and, as such, has significantly reduced its genome size and content. Although recent advances have allowed for transformation of C. trachomatis with an exogenous plasmid, genetic manipulation of Chlamydia remains challenging. In particular, the ability to create conditional knockouts has not been developed. This ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2018, 8:59]

Cited: 4 times

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A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning.

Macy G Olson, Megan Goldammer, Emilie Gauliard, Daniel Ladant, Scot P Ouellette,

The bacterial adenylate cyclase two-hybrid system (BACTH) is a genetic approach used to test protein interactions in vivo in E. coli. This system takes advantage of the two catalytic domains of Bordetella pertussis adenylate cyclase (CyaA) toxin, which can be fused separately to proteins of interest. If the proteins of ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2018, 1794:75-96]

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Analysis of Membrane Protein Interactions with a Bacterial Adenylate Cyclase-Based Two-Hybrid (BACTH) Technique.

Scot P Ouellette, Gouzel Karimova, Marilyne Davi, Daniel Ladant,

The bacterial two-hybrid (BACTH, for "Bacterial Adenylate Cyclase-based Two-Hybrid") technique is a simple and fast genetic approach to analyze protein-protein interactions in vivo. In this system, the proteins of interest are genetically fused to two complementary fragments from the catalytic domain of Bordetella pertussis adenylate cyclase and co-expressed in strains ... Read more >>

Curr Protoc Mol Biol (Current protocols in molecular biology)
[2017, 118:20.12.1-20.12.24]

Cited: 2 times

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Development of a Proximity Labeling System to Map the Chlamydia trachomatis Inclusion Membrane.

Elizabeth A Rucks, Macy G Olson, Lisa M Jorgenson, Rekha R Srinivasan, Scot P Ouellette,

Chlamydia grows within a membrane-bound vacuole termed an inclusion. The cellular processes that support the biogenesis and integrity of this pathogen-specified parasitic organelle are not understood. Chlamydia secretes integral membrane proteins called Incs that insert into the chlamydial inclusion membrane (IM). Incs contain at least two hydrophobic transmembrane domains flanked ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2017, 7:40]

Cited: 7 times

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Incidence of Wolbachia in aquatic insects.

Eric J Sazama, Michael J Bosch, Carmelita S Shouldis, Scot P Ouellette, Jeff S Wesner,

Wolbachia is a genus of intracellular bacteria typically found within the reproductive systems of insects that manipulates those systems of their hosts. While current estimates of Wolbachia incidence suggest that it infects approximately half of all arthropod species, these estimates are based almost entirely on terrestrial insects. No systematic survey ... Read more >>

Ecol Evol (Ecology and evolution)
[2017, 7(4):1165-1169]

Cited: 3 times

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Protein-Protein Interaction: Bacterial Two-Hybrid.

Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P Ouellette, Daniel Ladant,

The bacterial two-hybrid (BACTH, for "Bacterial Adenylate Cyclase-Based Two-Hybrid") system is a simple and fast genetic approach to detecting and characterizing protein-protein interactions in vivo. This system is based on the interaction-mediated reconstitution of a cyclic adenosine monophosphate (cAMP) signaling cascade in Escherichia coli. As BACTH uses a diffusible cAMP ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2017, 1615:159-176]

Cited: 2 times

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Tryptophan Codon-Dependent Transcription in Chlamydia pneumoniae during Gamma Interferon-Mediated Tryptophan Limitation.

Scot P Ouellette, Kelsey J Rueden, Elizabeth A Rucks,

In evolving to an obligate intracellular niche, Chlamydia has streamlined its genome by eliminating superfluous genes as it relies on the host cell for a variety of nutritional needs like amino acids. However, Chlamydia can experience amino acid starvation when the human host cell in which the bacteria reside is ... Read more >>

Infect. Immun. (Infection and immunity)
[2016, 84(9):2703-2713]

Cited: 5 times

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Correction: Polarized Cell Division of Chlamydia trachomatis.

Yasser Abdelrahman, Scot P Ouellette, Robert J Belland, John V Cox,

[This corrects the article DOI: 10.1371/journal.ppat.1005822.]. ... Read more >>

PLoS Pathog. (PLoS pathogens)
[2016, 12(8):e1005866]

Cited: 0 times

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Polarized Cell Division of Chlamydia trachomatis.

Yasser Abdelrahman, Scot P Ouellette, Robert J Belland, John V Cox,

Bacterial cell division predominantly occurs by a highly conserved process, termed binary fission, that requires the bacterial homologue of tubulin, FtsZ. Other mechanisms of bacterial cell division that are independent of FtsZ are rare. Although the obligate intracellular human pathogen Chlamydia trachomatis, the leading bacterial cause of sexually transmitted infections ... Read more >>

PLoS Pathog. (PLoS pathogens)
[2016, 12(8):e1005822]

Cited: 13 times

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Identification and Partial Characterization of Potential FtsL and FtsQ Homologs of Chlamydia.

Scot P Ouellette, Kelsey J Rueden, Yasser M AbdelRahman, John V Cox, Robert J Belland,

Chlamydia is amongst the rare bacteria that lack the critical cell division protein FtsZ. By annotation, Chlamydia also lacks several other essential cell division proteins including the FtsLBQ complex that links the early (e.g., FtsZ) and late (e.g., FtsI/Pbp3) components of the division machinery. Here, we report chlamydial FtsL and ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2015, 6:1264]

Cited: 5 times

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The trans-Golgi SNARE syntaxin 10 is required for optimal development of Chlamydia trachomatis.

Andrea L Lucas, Scot P Ouellette, Emily J Kabeiseman, Kyle H Cichos, Elizabeth A Rucks,

Chlamydia trachomatis, an obligate intracellular pathogen, grows inside of a vacuole, termed the inclusion. Within the inclusion, the organisms differentiate from the infectious elementary body (EB) into the reticulate body (RB). The RB communicates with the host cell through the inclusion membrane to obtain the nutrients necessary to divide, thus ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2015, 5:68]

Cited: 7 times

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Characterization of interactions between inclusion membrane proteins from Chlamydia trachomatis.

Emilie Gauliard, Scot P Ouellette, Kelsey J Rueden, Daniel Ladant,

Chlamydiae are obligate intracellular pathogens of eukaryotes. The bacteria grow in an intracellular vesicle called an inclusion, the membrane of which is heavily modified by chlamydial proteins called Incs (Inclusion membrane proteins). Incs represent 7-10% of the genomes of Chlamydia and, given their localization at the interface between the host ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2015, 5:13]

Cited: 11 times

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Reconceptualizing the chlamydial inclusion as a pathogen-specified parasitic organelle: an expanded role for Inc proteins.

Elizabeth R Moore, Scot P Ouellette,

Chlamydia is an obligate intracellular pathogen that develops in the host cell in a vacuole termed the chlamydial inclusion. The prevailing concept of the chlamydial inclusion is of a parasitophorous vacuole. Here, the inclusion is the recipient of one-way host-pathogen interactions thus draining nutrients from the cell and negatively impacting ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2014, 4:157]

Cited: 29 times

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Analysis of MreB interactors in Chlamydia reveals a RodZ homolog but fails to detect an interaction with MraY.

Scot P Ouellette, Kelsey J Rueden, Emilie Gauliard, Logan Persons, Piet A de Boer, Daniel Ladant,

Chlamydia is an obligate intracellular bacterial pathogen that has significantly reduced its genome in adapting to the intracellular environment. One class of genes for which the bacterium has few annotated examples is cell division, and Chlamydia lacks FtsZ, a central coordinator of the division apparatus. We have previously implicated MreB ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2014, 5:279]

Cited: 9 times

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A Gateway(®) -compatible bacterial adenylate cyclase-based two-hybrid system.

Scot P Ouellette, Emilie Gauliard, Zuzana Antosová, Daniel Ladant,

The bacterial adenylate cyclase two-hybrid (BACTH) system has been widely used to characterize protein-protein interactions in the prokaryotic world. This system relies on the interaction-mediated reconstitution of adenylate cyclase activity in Escherichia coli by bringing together two complementary fragments of the catalytic domain of the adenylate cyclase toxin of Bordetella ... Read more >>

Environ Microbiol Rep (Environmental microbiology reports)
[2014, 6(3):259-267]

Cited: 8 times

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Chlamydia co-opts the rod shape-determining proteins MreB and Pbp2 for cell division.

Scot P Ouellette, Gouzel Karimova, Agathe Subtil, Daniel Ladant,

Chlamydiae are obligate intracellular bacterial pathogens that have extensively reduced their genome in adapting to the intracellular environment. The chlamydial genome contains only three annotated cell division genes and lacks ftsZ. How this obligate intracellular pathogen divides is uncharacterized. Chlamydiae contain two high-molecular-weight (HMW) penicillin binding proteins (Pbp) implicated in ... Read more >>

Mol. Microbiol. (Molecular microbiology)
[2012, 85(1):164-178]

Cited: 40 times

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A Functional Slow Recycling Pathway of Transferrin is Required for Growth of Chlamydia.

Scot P Ouellette, Rey A Carabeo,

An inhibitor of host cell lysophospholipid acyltransferase, an enzyme involved in lipid metabolism blocked growth of the obligate intracellular pathogen Chlamydia through its action on the transport of transferrin (Tf) via the slow pathway of recycling. A detailed characterization of this inhibition revealed that Tf accumulated in vesicles positive for ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2010, 1:112]

Cited: 23 times

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Global transcriptional upregulation in the absence of increased translation in Chlamydia during IFNgamma-mediated host cell tryptophan starvation.

Scot P Ouellette, Thomas P Hatch, Yasser M AbdelRahman, Lorne A Rose, Robert J Belland, Gerald I Byrne,

The developmentally regulated intracellular pathogen Chlamydia pneumoniae is a natural tryptophan auxotroph. These organisms survive tryptophan starvation induced by host cell activation with IFNgamma by blocking maturation to the infectious form. In most bacteria, the stringent response is induced during amino acid starvation to promote survival. However, the response of ... Read more >>

Mol. Microbiol. (Molecular microbiology)
[2006, 62(5):1387-1401]

Cited: 51 times

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Different growth rates of Chlamydia trachomatis biovars reflect pathotype.

Isao Miyairi, Olaimatu S Mahdi, Scot P Ouellette, Robert J Belland, Gerald I Byrne,

Despite small genomic differences, Chlamydia trachomatis biovars exhibit diverse disease manifestations and different growth rates in vivo and in cell culture models.Chlamydial inclusion-forming units were enumerated over time in HeLa cells, to evaluate the length of the developmental cycle for C. trachomatis strains A, B, C, and E/Bour (ocular strains) ... Read more >>

J. Infect. Dis. (The Journal of infectious diseases)
[2006, 194(3):350-357]

Cited: 38 times

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The Chlamydia pneumoniae type III secretion-related lcrH gene clusters are developmentally expressed operons.

Scot P Ouellette, Yasser M Abdelrahman, Robert J Belland, Gerald I Byrne,

Two chlamydial homologues of the Yersinia lcrH chaperone for type III secretion system structural components are present within separate gene clusters. Quantitative transcriptional analyses demonstrated that each cluster is differentially regulated and expressed as an operon using major sigma factor elements, suggesting the presence of more elaborate developmental regulation mechanisms ... Read more >>

J. Bacteriol. (Journal of bacteriology)
[2005, 187(22):7853-7856]

Cited: 18 times

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Chlamydia pneumoniae and atherosclerosis.

Robert J Belland, Scot P Ouellette, Jens Gieffers, Gerald I Byrne,

Exposure to Chlamydia pneumoniae is extremely common, and respiratory infections occur repeatedly among most people. Strong associations exist between C. pneumoniae infection and atherosclerosis as demonstrated by: (i) sero-epidemiological studies showing that patients with cardiovascular disease have higher titres of anti-C. pneumoniae antibodies compared with control patients; (ii) detection of ... Read more >>

Cell. Microbiol. (Cellular microbiology)
[2004, 6(2):117-127]

Cited: 80 times

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