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Author Sara Montecchini

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An unusual cause of Steven-Johnson Syndrome.

Claudia De Guido, Adriana Calderaro, Maria Beatrice Ruozi, Valentina Maffini, Margherita Varini, Irene Lapetina, Monica Rubini, Sara Montecchini, Carlo Caffarelli, Icilio Dodi,

SJS is a rare mucocutaneous syndrome characterized by skin and mucous detachment. The main etiological factors are drugs and infections; sometimes the cause remains unknown. In the prodromal phase we observed non-specific symptoms, followed by mucocutaneous manifestation. Due to risk of complications and mortality a multidisciplinary approach is needed.  We ... Read more >>

Acta Biomed (Acta bio-medica : Atenei Parmensis)
[2020, 91(1):128-131]

Cited: 0 times

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Rapid microbial identification and phenotypic antimicrobial susceptibility testing directly from positive blood cultures: a new platform compared to routine laboratory methods.

Adriana Calderaro, Mirko Buttrini, Monica Martinelli, Silvia Covan, Sara Montecchini, Alberto Ruggeri, Maria Cristina Arcangeletti, Flora De Conto, Carlo Chezzi,

The Accelerate Pheno™ System (APS), a new platform that combines rapid identification (ID) of bacteria and yeasts and phenotypic antimicrobial susceptibility testing (AST) in a single assay, has been evaluated directly from positive blood cultures in comparison to routine laboratory methods. The APS ID results showed an overall sensitivity and ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2020, 96(3):114955]

Cited: 0 times

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Contribution of the FilmArray® Gastrointestinal Panel in the laboratory diagnosis of gastroenteritis in a cohort of children: a two-year prospective study.

Adriana Calderaro, Monica Martinelli, Mirko Buttrini, Sara Montecchini, Silvia Covan, Sabina Rossi, Francesca Ferraglia, Paolo Montagna, Federica Pinardi, Sandra Larini, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto,

This study represents a 2-year picture of the epidemiology of enteric pathogens in children suffering from gastroenteritis using the FilmArray® Gastrointestinal Panel (FA-GP), a multiplex molecular assay that allows to simultaneously detect a large panel of pathogens independently of the etiological suspicion and to evaluate its potential contribution to the ... Read more >>

Int. J. Med. Microbiol. (International journal of medical microbiology : IJMM)
[2018, 308(5):514-521]

Cited: 3 times

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Pleural tuberculosis: medical thoracoscopy greatly increases the diagnostic accuracy.

Angelo Gianni Casalini, Pier Anselmo Mori, Maria Majori, Miriam Anghinolfi, Enrico Maria Silini, Letizia Gnetti, Federica Motta, Sandra Larini, Sara Montecchini, Roberta Pisi, Adriana Calderaro,

Our objective was to evaluate the efficacy of a standardised work-up in the diagnosis of pleural tuberculosis (TB) that included fibreoptic bronchoscopy and medical thoracoscopy. A consecutive series of 52 pleural TB patients observed during the period 2001-2015 was evaluated retrospectively. 20 females, mean (range) age 39.7 (18-74) years, and 32 ... Read more >>

ERJ Open Res (ERJ open research)
[2018, 4(1):]

Cited: 2 times

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MALDI-TOF MS as a new tool for the identification of Dientamoeba fragilis.

Adriana Calderaro, Mirko Buttrini, Sara Montecchini, Sabina Rossi, Giovanna Piccolo, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto,

BACKGROUND:In this study for the first time, a Dientamoeba fragilis protein profile by MALDI-TOF MS was created in order to identify specific markers for the application of this technology in the laboratory diagnosis of dientamoebiasis. In particular, one D. fragilis reference strain was used to create a reference spectrum and ... Read more >>

Parasit Vectors (Parasites & vectors)
[2018, 11(1):11]

Cited: 1 time

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Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae.

Adriana Calderaro, Mirko Buttrini, Maddalena Piergianni, Sara Montecchini, Monica Martinelli, Silvia Covan, Giovanna Piccolo, Maria Cristina Medici, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto,

Carbapenem-resistant Enterobacteriaceae (CRE) have spread globally and represent a serious and growing threat to public health. The introduction of rapid and sensitive methods for the detection of carbapenemase-producing bacteria is of increasing importance. The carbapenemase production can be detected using non-molecular methods (such as the modified Hodge test, the synergy ... Read more >>

PLoS ONE (PloS one)
[2017, 12(4):e0174908]

Cited: 5 times

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MALDI-TOF mass spectrometry as a potential tool for Trichomonas vaginalis identification.

Adriana Calderaro, Maddalena Piergianni, Sara Montecchini, Mirko Buttrini, Giovanna Piccolo, Sabina Rossi, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto,

Trichomonas vaginalis is a flagellated protozoan causing trichomoniasis, a sexually transmitted human infection, with around 276.4 million new cases estimated by World Health Organization. Culture is the gold standard method for the diagnosis of T. vaginalis infection. Recently, immunochromatographic assays as well as PCR assays for the detection of T. ... Read more >>

BMC Infect. Dis. (BMC infectious diseases)
[2016, 16:261]

Cited: 4 times

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Diagnostic performances of antigen detection compared to conventional and nucleic acid detection of Entamoeba histolytica in a non-endemic setting.

Adriana Calderaro, Maddalena Piergianni, Giovanna Piccolo, Sabina Rossi, Sara Montecchini, Mirko Buttrini, Maria Cristina Arcangeletti, Maria Cristina Medici, Carlo Chezzi, Flora De Conto,

This study evaluated the immunochromatographic (IC) assay "TECHLAB(®) E. HISTOLYTICA QUIK CHEK™" analysing 36 faecal samples and 7 cultured strains. This assay was compared to the methods performed in our laboratory for the diagnosis of amoebiasis. The IC assay revealed a detection limit of 103 trophozoites/g faeces and no cross-reactivity ... Read more >>

New Microbiol. (The new microbiologica)
[2016, 39(2):153-155]

Cited: 0 times

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Higher recovery rate of microorganisms from cerebrospinal fluid samples by the BACTEC culture system in comparison with agar culture.

Adriana Calderaro, Monica Martinelli, Sara Montecchini, Federica Motta, Silvia Covan, Sandra Larini, Maria Cristina Medici, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto,

The aim of this study was to assess the diagnostic value of the BACTEC FX blood culture (BC) system as compared to the agar culture (AC) of cerebrospinal fluid samples (CSF), evaluating the recovery rate and the time to detection of microorganisms in a 3.5-year period. From December 2011 to ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2016, 84(4):281-286]

Cited: 2 times

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MALDI-TOF mass spectrometry for the detection and differentiation of Entamoeba histolytica and Entamoeba dispar.

Adriana Calderaro, Maddalena Piergianni, Mirko Buttrini, Sara Montecchini, Giovanna Piccolo, Chiara Gorrini, Sabina Rossi, Carlo Chezzi, Maria Cristina Arcangeletti, Maria Cristina Medici, Flora De Conto,

Detection of Entamoeba histolytica and its differentiation from Entamoeba dispar is an important goal of the clinical parasitology laboratory. The aim of this study was the identification and differentiation of E. histolytica and E. dispar by MALDI-TOF MS, in order to evaluate the application of this technique in routine diagnostic ... Read more >>

PLoS ONE (PloS one)
[2015, 10(4):e0122448]

Cited: 8 times

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Identification of Dermatophyte species after implementation of the in-house MALDI-TOF MS database.

Adriana Calderaro, Federica Motta, Sara Montecchini, Chiara Gorrini, Giovanna Piccolo, Maddalena Piergianni, Mirko Buttrini, Maria Cristina Medici, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto,

Despite that matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a powerful tool in the clinical microbiology setting, few studies have till now focused on MALDI-TOF MS-based identification of dermatophytes. In this study, we analyze dermatophytes strains isolated from clinical samples by MALDI-TOF MS to supplement the reference ... Read more >>

Int J Mol Sci (International journal of molecular sciences)
[2014, 15(9):16012-16024]

Cited: 12 times

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Leptospira species and serovars identified by MALDI-TOF mass spectrometry after database implementation.

Adriana Calderaro, Giovanna Piccolo, Chiara Gorrini, Sara Montecchini, Mirko Buttrini, Sabina Rossi, Maddalena Piergianni, Flora De Conto, Maria Cristina Arcangeletti, Carlo Chezzi, Maria Cristina Medici,

BACKGROUND: Leptospirosis, a spirochaetal zoonotic disease of worldwide distribution, endemic in Europe, has been recognized as an important emerging infectious disease, though yet it is mostly a neglected disease which imparts its greatest burden on impoverished populations from developing countries. Leptospirosis is caused by the infection with any of the ... Read more >>

BMC Res Notes (BMC research notes)
[2014, 7:330]

Cited: 8 times

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Intestinal parasitoses in a tertiary-care hospital located in a non-endemic setting during 2006-2010.

Adriana Calderaro, Sara Montecchini, Sabina Rossi, Chiara Gorrini, Flora De Conto, Maria Cristina Medici, Carlo Chezzi, Maria Cristina Arcangeletti,

BACKGROUND: The aim of this study was to assess the epidemiology of intestinal parasitoses during a 5-year period in patients attending a tertiary-care hospital in a non-endemic setting. METHODS: In the period 2006-2010, 15,752 samples from 8,886 patients with clinically suspected parasitosis were subjected to macroscopic and microscopic examination, to ... Read more >>

BMC Infect. Dis. (BMC infectious diseases)
[2014, 14:264]

Cited: 10 times

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Accurate identification of the six human Plasmodium spp. causing imported malaria, including Plasmodium ovale wallikeri and Plasmodium knowlesi.

Adriana Calderaro, Giovanna Piccolo, Chiara Gorrini, Sabina Rossi, Sara Montecchini, Maria Loretana Dell'Anna, Flora De Conto, Maria Cristina Medici, Carlo Chezzi, Maria Cristina Arcangeletti,

BACKGROUND: Accurate identification of Plasmodium infections in non-endemic countries is of critical importance with regard to the administration of a targeted therapy having a positive impact on patient health and management and allowing the prevention of the risk of re-introduction of endemic malaria in such countries. Malaria is no longer ... Read more >>

Malar. J. (Malaria journal)
[2013, 12:321]

Cited: 29 times

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A 22-year survey of leishmaniasis cases in a tertiary-care hospital in an endemic setting.

Adriana Calderaro, Sara Montecchini, Sabina Rossi, Chiara Gorrini, Maria Loretana Dell'Anna, Giovanna Piccolo, Maria Cristina Medici, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto,

The northward spread of leishmaniasis from Mediterranean to Continental Europe affects our area where it is typically associated with Leishmania infantum infection. In this study a 22-year survey was performed in patients (including both patients with and without history of travel through endemic areas other than Italy) attending the University ... Read more >>

Int J Environ Res Public Health (International journal of environmental research and public health)
[2014, 11(3):2834-2845]

Cited: 2 times

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Identification of Borrelia species after creation of an in-house MALDI-TOF MS database.

Adriana Calderaro, Chiara Gorrini, Giovanna Piccolo, Sara Montecchini, Mirko Buttrini, Sabina Rossi, Maddalena Piergianni, Maria Cristina Arcangeletti, Flora De Conto, Carlo Chezzi, Maria Cristina Medici,

Lyme borreliosis (LB) is a multisystemic disease caused by Borrelia burgdorferi sensu lato (sl) complex transmitted to humans by Ixodes ticks. B. burgdorferi sl complex, currently comprising at least 19 genospecies, includes the main pathogenic species responsible for human disease in Europe: B. burgdorferi sensu stricto (ss), B. afzelii, and ... Read more >>

PLoS ONE (PloS one)
[2014, 9(2):e88895]

Cited: 18 times

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Two rare cases of Acremonium acute endophthalmitis after cataract surgery in a tertiary care hospital.

Adriana Calderaro, Federica Motta, Sandra Larini, Chiara Gorrini, Monica Martinelli, Giovanni Piscopo, Magda Benecchi, Maria Cristina Arcangeletti, Maria Cristina Medici, Flora De Conto, Sara Montecchini, Alberto Neri, Patrizia Scaroni, Stefano Gandolfi, Carlo Chezzi,

This report describes two cases of Acremonium sp. endophthalmitis, occurring in two patients who underwent cataract surgery on the same day in the same operating room of our hospital ophthalmology clinic. Diagnosis of fungal endophthalmitis was established by the repeated isolation of the same fungal agent from vitreous washing, acqueous ... Read more >>

New Microbiol. (The new microbiologica)
[2013, 36(4):427-431]

Cited: 1 time

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Comparative analysis of different methods to detect Clostridium difficile infection.

Adriana Calderaro, Mirko Buttrini, Monica Martinelli, Chiara Gorrini, Sara Montecchini, Maria Cristina Medici, Maria Cristina Arcangeletti, Flora De Conto, Silvia Covan, Carlo Chezzi,

The increased incidence and severity of Clostridium difficile infection, particularly in North America and Europe, have brought renewed focus on the most appropriate method to detect C. difficile and/or its toxins in stools. This prospective study evaluated the usefulness of the Illumigene TM C. difficile assay in diagnostic practice for ... Read more >>

New Microbiol. (The new microbiologica)
[2013, 36(1):57-63]

Cited: 2 times

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MALDI-TOF MS analysis of human and animal Brachyspira species and benefits of database extension.

Adriana Calderaro, Giovanna Piccolo, Sara Montecchini, Mirko Buttrini, Chiara Gorrini, Sabina Rossi, Maria Cristina Arcangeletti, Flora De Conto, Maria Cristina Medici, Carlo Chezzi,

Spirochaetes belonging to the genus Brachyspira are anaerobic bacteria that colonize the large intestine of humans and animals, mainly pigs. The main species are namely, B. hyodysenteriae, the etiological agent of swine dysentery, B. pilosicoli, a zoonotic agent causing colonic spirochaetosis both in humans and different animal species, B. aalborgi, ... Read more >>

J Proteomics (Journal of proteomics)
[2013, 78:273-280]

Cited: 18 times

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A new real-time PCR for the detection of Plasmodium ovale wallikeri.

Adriana Calderaro, Giovanna Piccolo, Chiara Gorrini, Sara Montecchini, Sabina Rossi, Maria Cristina Medici, Carlo Chezzi, Georges Snounou,

It has been proposed that ovale malaria in humans is caused by two closely related but distinct species of malaria parasites: P. ovale curtisi and P. ovale wallikeri. We have extended and optimized a Real-time PCR assay targeting the parasite's small subunit ribosomal RNA (ssrRNA) gene to detect both these ... Read more >>

PLoS ONE (PloS one)
[2012, 7(10):e48033]

Cited: 14 times

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Intestinal spirochaetosis associated with hyperplastic and adenomatous colonic polyps.

Adriana Calderaro, Chiara Gorrini, Sara Montecchini, Vincenzo Villanacci, Gabrio Bassotti, Giuseppe Dettori, Carlo Chezzi,

Intestinal spirochaetosis is a human colonic infection due to Brachyspira aalborgi and Brachyspira pilosicoli causing various abdominal complaints. Although the presence of healthy epithelial cells was hypothesized to be essential for the adhesion of spirochaetes to the colonic mucosa, their adhesion to hyperplastic and adenomatous colonic polyps has been observed ... Read more >>

Pathol. Res. Pract. (Pathology, research and practice)
[2012, 208(3):177-180]

Cited: 6 times

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Similar diagnostic performances of antigen detection and nucleic acid detection of Cryptosporidium spp. in a low-prevalence setting.

Adriana Calderaro, Sara Montecchini, Chiara Gorrini, Giuseppe Dettori, Carlo Chezzi,

The diagnostic value of a real-time PCR assay for the detection of Cryptosporidium spp. in fecal samples was assessed as compared to the combination of the immunocromatographic assay (IC) and immunofluorescence assay (IF) currently performed in our laboratory for the diagnosis of cryptosporidiosis. On a total of 1040 samples collected ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2011, 70(1):72-77]

Cited: 7 times

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Presence of anti-Borrelia burgdorferi antibodies and Borrelia burgdorferi sensu lato DNA in samples of subjects in an area of the Northern Italy in the period 2002-2008.

Adriana Calderaro, Sara Montecchini, Chiara Gorrini, Giovanna Piccolo, Carlo Chezzi, Giuseppe Dettori,

Lyme borreliosis (LB) is a multisystemic disease caused by Borrelia burgdorferi sensu lato complex transmitted to humans by Ixodes ticks and whose epidemiology is poorly investigated in Europe. In this study an epidemiologic survey on the prevalence of the causative agent of such infectious disease was performed in the area ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2011, 70(4):455-460]

Cited: 5 times

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Evaluation of a real-time polymerase chain reaction assay for the detection of Dientamoeba fragilis.

Adriana Calderaro, Chiara Gorrini, Sara Montecchini, Simona Peruzzi, Giovanna Piccolo, Sabina Rossi, Franco Gargiulo, Nino Manca, Giuseppe Dettori, Carlo Chezzi,

The diagnostic value of a real-time polymerase chain reaction (PCR) assay targeting the 5.8S rDNA of Dientamoeba fragilis was investigated as compared with conventional parasitologic methods including cultivation testing 959 fecal samples from 491 patients attending a tertiary-care hospital and suspected of having an intestinal parasitosis. The real-time PCR assay ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2010, 67(3):239-245]

Cited: 17 times

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Evaluation of a real-time polymerase chain reaction assay for the laboratory diagnosis of giardiasis.

Adriana Calderaro, Chiara Gorrini, Sara Montecchini, Simona Peruzzi, Giovanna Piccolo, Sabina Rossi, Franco Gargiulo, Nino Manca, Giuseppe Dettori, Carlo Chezzi,

A real-time polymerase chain reaction (PCR) assay was evaluated in comparison with the combination of conventional methods (microscopic examination and antigen detection assay) during the period 2006 to 2008 on 771 fecal samples belonging to 386 patients to assess its usefulness for an accurate laboratory diagnosis of giardiasis. The real-time ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2010, 66(3):261-267]

Cited: 20 times

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