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Author Sara Deola

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A modular framework for the development of targeted Covid-19 blood transcript profiling panels.

Darawan Rinchai, Basirudeen Syed Ahamed Kabeer, Mohammed Toufiq, Zohreh Tatari-Calderone, Sara Deola, Tobias Brummaier, Mathieu Garand, Ricardo Branco, Nicole Baldwin, Mohamed Alfaki, Matthew C Altman, Alberto Ballestrero, Matteo Bassetti, Gabriele Zoppoli, Andrea De Maria, Benjamin Tang, Davide Bedognetti, Damien Chaussabel,

<h4>Background</h4>Covid-19 morbidity and mortality are associated with a dysregulated immune response. Tools are needed to enhance existing immune profiling capabilities in affected patients. Here we aimed to develop an approach to support the design of targeted blood transcriptome panels for profiling the immune response to SARS-CoV-2 infection.<h4>Methods</h4>We designed a pool ... Read more >>

J Transl Med (Journal of translational medicine)
[2020, 18(1):291]

Cited: 4 times

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A modular framework for the development of targeted Covid-19 blood transcript profiling panels

Darawan Rinchai, Basirudeen Kabeer, Mohammed Toufiq, Zohreh Tatari-Calderone, Sara Deola, Tobias Brummaier, Mathieu Garand, Ricardo Branco, Nicole Baldwin, Mohamed Alfaki, Matthew Altman, Alberto Ballestrero, Matteo Bassetti, Gabriele Zoppoli, Andrea De Maria, Benjamin Tang, Davide Bedognetti, Damien Chaussabel,

<h4>Background: </h4> Covid-19 morbidity and mortality are associated with a dysregulated immune response. Tools are needed to enhance existing immune profiling capabilities in affected patients. Here we aimed to develop an approach to support the design of focused blood transcriptome panels for profiling the immune response to SARS-CoV-2 infection. <h4>Methods:</h4> ... Read more >>

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A modular framework for the development of targeted Covid-19 blood transcript profiling panels

Darawan Rinchai, Basirudeen Kabeer, Mohammed Toufiq, Zohreh Calderone, Sara Deola, Tobias Brummaier, Mathieu Garand, Ricardo Branco, Nicole Baldwin, Mohamed Alfaki, Matthew Altman, Alberto Ballestrero, Matteo Bassetti, Gabriele Zoppoli, Andrea De Maria, Benjamin Tang, Davide Bedognetti, Damien Chaussabel,

<h4>SUMMARY</h4> Covid-19 morbidity and mortality are associated with a dysregulated immune response. Tools are needed to enhance existing immune profiling capabilities in affected patients. Here we aimed to develop an approach to support the design of focused blood transcriptome panels for profiling the immune response to SARS-CoV-2 infection. We designed ... Read more >>

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Influence of storage conditions of small volumes of blood on immune transcriptomic profiles.

Rebecca Mathew, Mohammed Toufiq, Valentina Mattei, Muna Al Hashmi, Harshitha Shobha Manjunath, Basirudeen Syed Ahamed Kabeer, Rita Calzone, Chiara Cugno, Damien Chaussabel, Sara Deola, Sara Tomei,

<h4>Objective</h4>Transcriptome analysis of human whole blood is used to discover biomarkers of diseases and to assess phenotypic traits. Here we have collected small volumes of blood in Tempus solution and tested whether different storage conditions have an impact on transcriptomic profiling. Fifty µl of blood were collected in 100µl of ... Read more >>

BMC Res Notes (BMC research notes)
[2020, 13(1):150]

Cited: 0 times

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Flow-Cytometry Platform for Intracellular Detection of FVIII in Blood Cells: A New Tool to Assess Gene Therapy Efficiency for Hemophilia A.

Muhammad Elnaggar, Anjud Al-Mohannadi, Dhanya Kizhakayil, Christophe Michel Raynaud, Sharefa Al-Mannai, Giusy Gentilcore, Igor Pavlovski, Abbirami Sathappan, Nicholas Van Panhuys, Chiara Borsotti, Antonia Follenzi, Jean-Charles Grivel, Sara Deola,

Detection of factor VIII (FVIII) in cells by flow cytometry is controversial, and no monoclonal fluorescent antibody is commercially available. In this study, we optimized such an assay and successfully used it as a platform to study the functional properties of phosphoglycerate kinase (PGK)-FVIII lentiviral vector-transduced cells by directly visualizing ... Read more >>

Mol Ther Methods Clin Dev (Molecular therapy. Methods & clinical development)
[2020, 17:1-12]

Cited: 1 time

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Clofarabine and Treosulfan as Conditioning for Matched Related and Unrelated Hematopoietic Stem Cell Transplantation: Results from the Clo3o Phase II Trial.

Jacopo Peccatori, Sara Mastaglio, Fabio Giglio, Raffaella Greco, Roberto Crocchiolo, Francesca Patriarca, Barbara Forno, Sara Deola, Andrea Assanelli, Maria Teresa Lupo Stanghellini, Magda Marcatti, Marco Zecca, Sergio Cortelazzo, Renato Fanin, Franca Fagioli, Franco Locatelli, Fabio Ciceri,

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) can be curative for patients with hematologic malignancies. The ideal conditioning regimen before allo-HSCT has not been established. We conducted a Phase II study to evaluate the tolerability and efficacy of clofarabine and treosulfan as conditioning regimen before allo-HSCT. The primary objective was to ... Read more >>

Biol Blood Marrow Transplant (Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation)
[2020, 26(2):316-322]

Cited: 1 time

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Tailoring cells for clinical needs: Meeting report from the Advanced Therapy in Healthcare symposium (October 28-29 2017, Doha, Qatar).

Sara Deola, Bella S Guerrouahen, Heba Sidahmed, Anjud Al-Mohannadi, Muhammad Elnaggar, Ramaz Elsadig, Essam M Abdelalim, Goran Petrovski, Goran Petrovski, Massimo Gadina, Adrian Thrasher, Winfried S Wels, Stephen P Hunger, Ena Wang, Francesco M Marincola, , Cristina Maccalli, Chiara Cugno,

New technologies and therapies designed to facilitate development of personalized treatments are rapidly emerging in the field of biomedicine. Strikingly, the goal of personalized medicine refined the concept of therapy by developing cell-based therapies, the so-called "living drugs". Breakthrough advancements were achieved in this regard in the fields of gene ... Read more >>

J Transl Med (Journal of translational medicine)
[2018, 16(1):276]

Cited: 1 time

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Phase II Study of Sequential Infusion of Donor Lymphocyte Infusion and Cytokine-Induced Killer Cells for Patients Relapsed after Allogeneic Hematopoietic Stem Cell Transplantation.

Martino Introna, Federico Lussana, Alessandra Algarotti, Elisa Gotti, Rut Valgardsdottir, Caterina Micò, Anna Grassi, Chiara Pavoni, Maria Luisa Ferrari, Federica Delaini, Elisabetta Todisco, Irene Cavattoni, Sara Deola, Ettore Biagi, Adriana Balduzzi, Attilio Rovelli, Matteo Parma, Sara Napolitano, Giusy Sgroi, Emanuela Marrocco, Paolo Perseghin, Daniela Belotti, Benedetta Cabiati, Giuseppe Gaipa, Josée Golay, Andrea Biondi, Alessandro Rambaldi,

Seventy-four patients who relapsed after allogeneic stem cell transplantation were enrolled in a phase IIA study and treated with the sequential infusion of donor lymphocyte infusion (DLI) followed by cytokine-induced killer (CIK) cells. Seventy-three patients were available for the intention to treat analysis. At least 1 infusion of CIK cells ... Read more >>

Biol Blood Marrow Transplant (Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation)
[2017, 23(12):2070-2078]

Cited: 13 times

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Two hits in one: whole genome sequencing unveils LIG4 syndrome and urofacial syndrome in a case report of a child with complex phenotype.

Abeer Fadda, Fiza Butt, Sara Tomei, Sara Deola, Bernice Lo, Amal Robay, Alya Al-Shakaki, Noor Al-Hajri, Ronald Crystal, Marios Kambouris, Ena Wang, Francesco M Marincola, Khalid A Fakhro, Chiara Cugno,

<h4>Background</h4>Ligase IV syndrome, a hereditary disease associated with compromised DNA damage response mechanisms, and Urofacial syndrome, caused by an impairment of neural cell signaling, are both rare genetic disorders, whose reports in literature are limited. We describe the first case combining both disorders in a specific phenotype.<h4>Case presentation</h4>We report a ... Read more >>

BMC Med Genet (BMC medical genetics)
[2016, 17(1):84]

Cited: 9 times

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Granulocyte transfusions in children and adults with hematological malignancies: benefits and controversies.

Chiara Cugno, Sara Deola, Perla Filippini, David F Stroncek, Sergio Rutella,

Bacterial and fungal infections continue to pose a major clinical challenge in patients with prolonged severe neutropenia after chemotherapy or hematopoietic stem cell transplantation (HSCT). With the advent of granulocyte colony-stimulating factor (G-CSF) to mobilize neutrophils in healthy donors, granulocyte transfusions have been broadly used to prevent and/or treat life-threatening ... Read more >>

J Transl Med (Journal of translational medicine)
[2015, 13:362]

Cited: 9 times

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Abnormal cervical cytology after allogeneic bone marrow transplantation.

Giovanni Negri, Martina Herz, Sara Deola, Andrea Piccin, Marco Casini, Bianca Babich, Martina Tauber, Sergio Messini, Maria Raffaella Marucci, Fabio Vittadello,

<h4>Objectives</h4>Allogeneic bone marrow transplantation (BMT) is a procedure mostly used for high-risk hematologic malignances. In women, follow-up protocols after BMT include gynecologic checkups with Papanicolaou (Pap) smears.<h4>Methods</h4>We evaluated 117 Pap smears in 54 women who underwent allogeneic BMT and correlated the smear morphology with the BMT-related medical treatment.<h4>Results</h4>Abnormal Pap smears ... Read more >>

Am J Clin Pathol (American journal of clinical pathology)
[2014, 142(2):222-226]

Cited: 1 time

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Treatment of graft versus host disease with mesenchymal stromal cells: a phase I study on 40 adult and pediatric patients.

Martino Introna, Giovanna Lucchini, Erica Dander, Stefania Galimberti, Attilio Rovelli, Adriana Balduzzi, Daniela Longoni, Fabio Pavan, Francesca Masciocchi, Alessandra Algarotti, Caterina Micò, Anna Grassi, Sara Deola, Irene Cavattoni, Giuseppe Gaipa, Daniela Belotti, Paolo Perseghin, Matteo Parma, Enrico Pogliani, Josee Golay, Olga Pedrini, Chiara Capelli, Sergio Cortelazzo, Giovanna D'Amico, Andrea Biondi, Alessandro Rambaldi, Ettore Biagi,

This phase I multicenter study was aimed at assessing the feasibility and safety of intravenous administration of third party bone marrow-derived mesenchymal stromal cells (MSC) expanded in platelet lysate in 40 patients (15 children and 25 adults), experiencing steroid-resistant grade II to IV graft-versus-host disease (GVHD). Patients received a median ... Read more >>

Biol Blood Marrow Transplant (Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation)
[2014, 20(3):375-381]

Cited: 95 times

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Next generation sequencing: new tools in immunology and hematology.

Antonio Mori, Sara Deola, Luciano Xumerle, Vladan Mijatovic, Giovanni Malerba, Vladia Monsurrò,

One of the hallmarks of the adaptive immune system is the specificity of B and T cell receptors. Thanks to somatic recombination, a large repertoire of receptors can be generated within an individual that guarantee the recognition of a vast number of antigens. Monoclonal antibodies have limited applicability, given the ... Read more >>

Blood Res (Blood research)
[2013, 48(4):242-249]

Cited: 22 times

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Genomic loss of patient-specific HLA in acute myeloid leukemia relapse after well-matched unrelated donor HSCT.

Cristina Toffalori, Irene Cavattoni, Sara Deola, Sara Mastaglio, Fabio Giglio, Benedetta Mazzi, Andrea Assanelli, Jacopo Peccatori, Claudio Bordignon, Chiara Bonini, Sergio Cortelazzo, Fabio Ciceri, Katharina Fleischhauer, Luca Vago,

Blood (Blood)
[2012, 119(20):4813-4815]

Cited: 21 times

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Bendamustine salvage for the treatment of relapsed Hodgkin's lymphoma after allogeneic bone marrow transplantation.

Michael Mian, Mohsen Farsad, Norbert Pescosta, Marco Casini, Irene Maria Cavattoni, Sara Deola, Sergio Cortelazzo,

Ann Hematol (Annals of hematology)
[2013, 92(1):121-123]

Cited: 6 times

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Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS.

Silvia Selleri, Marco Palazzo, Sara Deola, Ena Wang, Andrea Balsari, Francesco M Marincola, Cristiano Rumio,

Enteroendocrine cells are hormone-secreting cells spread along the intestinal epithelium. Their principal function is to promote the digestion of food. However, little is known about other functions that these cells may play, since they are difficult to study as a whole endocrine organ due to their diffuse localization. It is ... Read more >>

Int Immunol (International immunology)
[2008, 20(8):961-970]

Cited: 26 times

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GM-CSF/IL-3/IL-5 receptor common beta chain (CD131) expression as a biomarker of antigen-stimulated CD8+ T cells.

Silvia Selleri, Sara Deola, Zoltan Pos, Ping Jin, Andrea Worschech, Stefanie L Slezak, Cristiano Rumio, Monica C Panelli, Dragan Maric, David F Stroncek, Ena Wang, Francesco M Marincola,

<h4>Background</h4>Upon Ag-activation cytotoxic T cells (CTLs) produce IFN-gamma GM-CSF and TNF-alpha, which deliver simultaneously pro-apoptotic and pro-inflammatory signals to the surrounding microenvironment. Whether this secretion affects in an autocrine loop the CTLs themselves is unknown.<h4>Methods</h4>Here, we compared the transcriptional profile of Ag-activated, Flu-specific CTL stimulated with the FLU M1:58-66 peptide ... Read more >>

J Transl Med (Journal of translational medicine)
[2008, 6:17]

Cited: 6 times

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Helper B cells promote cytotoxic T cell survival and proliferation independently of antigen presentation through CD27/CD70 interactions.

Sara Deola, Monica C Panelli, Dragan Maric, Silvia Selleri, Natalia I Dmitrieva, Ching Y Voss, Harvey Klein, David Stroncek, Ena Wang, Francesco M Marincola,

CD8-expressing cytotoxic T cell (CTL) interactions with APCs and helper T cells determine their function and ability to survive. In this study, we describe a novel interaction independent of Ag presentation between activated CTLs and bystander CD19-expressing B lymphocytes. Ag-stimulated CTLs serially engage autologous B lymphocytes through CD27/CD70 contact that ... Read more >>

J Immunol (Journal of immunology (Baltimore, Md. : 1950))
[2008, 180(3):1362-1372]

Cited: 37 times

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Molecular purging of multiple myeloma cells by ex-vivo culture and retroviral transduction of mobilized-blood CD34+ cells.

Sara Deola, Samantha Scaramuzza, Roberto Sciarretta Birolo, Massimiliano Cergnul, Francesca Ficara, Jonathan Dando, Claudia Voena, Sergio Vai, Marta Monari, Enrico Pogliani, Gianmarco Corneo, Jacopo Peccatori, Silvia Selleri, Claudio Bordignon, Maria Grazia Roncarolo, Alessandro Aiuti, Marco Bregni,

<h4>Background</h4>Tumor cell contamination of the apheresis in multiple myeloma is likely to affect disease-free and overall survival after autografting.<h4>Objective</h4>To purge myeloma aphereses from tumor contaminants with a novel culture-based purging method.<h4>Methods</h4>We cultured myeloma-positive CD34+ PB samples in conditions that retained multipotency of hematopoietic stem cells, but were unfavourable to survival ... Read more >>

J Transl Med (Journal of translational medicine)
[2007, 5:35]

Cited: 5 times

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Molecular signatures induced by interleukin-2 on peripheral blood mononuclear cells and T cell subsets.

Ping Jin, Ena Wang, Maurizio Provenzano, Sara Deola, Silvia Selleri, Jiaqiang Ren, Sonia Voiculescu, David Stroncek, Monica C Panelli, Francesco M Marincola,

Experimentally, interleukin-2 (IL-2) exerts complex immunological functions promoting the proliferation, survival and activation of T cells on one hand and inducing immune regulatory mechanisms on the other. This complexity results from a cross talk among immune cells which sways the effects of IL-2 according to the experimental or clinical condition ... Read more >>

J Transl Med (Journal of translational medicine)
[2006, 4:26]

Cited: 25 times

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Polarized monocyte response to cytokine stimulation.

Dirk Nagorsen, Sara Deola, Kina Smith, Ena Wang, Vladia Monsurro, Paola Zanovello, Francesco M Marincola, Monica C Panelli,

<h4>Background</h4>Mononuclear phagocytes (MPs) stand at the crossroads between the induction of acute inflammation to recruit and activate immune effector cells and the downmodulation of the inflammatory process to contain collateral damage. This decision is extensively modulated by the cytokine microenvironment, which includes a broad array of cytokines whose direct effect ... Read more >>

Genome Biol (Genome biology)
[2005, 6(2):R15]

Cited: 20 times

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IL-3 or IL-7 increases ex vivo gene transfer efficiency in ADA-SCID BM CD34+ cells while maintaining in vivo lymphoid potential.

Francesca Ficara, Daniela B Superchi, Raisa Jofra Hernández, Cristina Mocchetti, Nicole Carballido-Perrig, Grazia Andolfi, Sara Deola, Augusto Colombo, Claudio Bordignon, José M Carballido, Maria Grazia Roncarolo, Alessandro Aiuti,

To improve maintenance and gene transfer of human lymphoid progenitors for clinical use in gene therapy of adenosine deaminase (ADA)-deficient SCID we investigated several gene transfer protocols using various stem cell-enriched sources. The lymphoid differentiation potential was measured by an in vitro clonal assay for B/NK cells and in the ... Read more >>

Mol Ther (Molecular therapy : the journal of the American Society of Gene Therapy)
[2004, 10(6):1096-1108]

Cited: 10 times

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Efficient gene transfer into primitive hematopoietic progenitors using a bone marrow microenvironment cell line engineered to produce retroviral vectors.

Jonathan S Dando, Francesca Ficara, Sara Deola, Maria Grazia Roncarolo, Claudio Bordignon, Alessandro Aiuti,

<h4>Background and objectives</h4>Effective gene transfer into human hematopoietic stem/progenitor cells is a compromise between achieving high transduction efficiency and maintaining the desired biological characteristics of the target cell. The aim of our work was to exploit the stromal microenvironment to increase gene transfer and maintenance of hematopoietic progenitors.<h4>Design and methods</h4>The ... Read more >>

Haematologica (Haematologica)
[2004, 89(4):462-470]

Cited: 0 times

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Mobilized blood CD34+ cells transduced and selected with a clinically applicable protocol reconstitute lymphopoiesis in SCID-Hu mice.

Sara Deola, Samantha Scaramuzza, Roberto Sciarretta Birolo, Nicole Carballido-Perrig, Francesca Ficara, Cristina Mocchetti, Jonathan Dando, José M Carballido, Claudio Bordignon, Maria Grazia Roncarolo, Marco Bregni, Alessandro Aiuti,

We developed a clinically applicable gene transfer procedure into mobilized peripheral blood (MPB) CD34(+) hematopoietic progenitor cells, based on single viral exposure and selection of engineered cells. CD34(+) cells were transduced with a retroviral vector carrying the truncated form of the nerve growth factor receptor (Delta NGFR) marker gene, and ... Read more >>

Hum Gene Ther (Human gene therapy)
[2004, 15(3):305-311]

Cited: 10 times

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Correction of ADA-SCID by stem cell gene therapy combined with nonmyeloablative conditioning.

Alessandro Aiuti, Shimon Slavin, Memet Aker, Francesca Ficara, Sara Deola, Alessandra Mortellaro, Shoshana Morecki, Grazia Andolfi, Antonella Tabucchi, Filippo Carlucci, Enrico Marinello, Federica Cattaneo, Sergio Vai, Paolo Servida, Roberto Miniero, Maria Grazia Roncarolo, Claudio Bordignon,

Hematopoietic stem cell (HSC) gene therapy for adenosine deaminase (ADA)-deficient severe combined immunodeficiency (SCID) has shown limited clinical efficacy because of the small proportion of engrafted genetically corrected HSCs. We describe an improved protocol for gene transfer into HSCs associated with nonmyeloablative conditioning. This protocol was used in two patients ... Read more >>

Science (Science (New York, N.Y.))
[2002, 296(5577):2410-2413]

Cited: 607 times

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