Full Text Journal Articles by
Author Robert J Belland


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Comparative Transcriptional Analyses of Francisella tularensis and Francisella novicida.

Siva T Sarva, Robert H Waldo, Robert J Belland, Karl E Klose,

Francisella tularensis is composed of a number of subspecies with varied geographic distribution, host ranges, and virulence. In view of these marked differences, comparative functional genomics may elucidate some of the molecular mechanism(s) behind these differences. In this study a shared probe microarray was designed that could be used to ... Read more >>

PLoS ONE (PloS one)
[2016, 11(8):e0158631]

Cited: 0 times

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Correction: Polarized Cell Division of Chlamydia trachomatis.

Yasser Abdelrahman, Scot P Ouellette, Robert J Belland, John V Cox,

[This corrects the article DOI: 10.1371/journal.ppat.1005822.]. ...

PLoS Pathog. (PLoS pathogens)
[2016, 12(8):e1005866]

Cited: 0 times

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Polarized Cell Division of Chlamydia trachomatis.

Yasser Abdelrahman, Scot P Ouellette, Robert J Belland, John V Cox,

Bacterial cell division predominantly occurs by a highly conserved process, termed binary fission, that requires the bacterial homologue of tubulin, FtsZ. Other mechanisms of bacterial cell division that are independent of FtsZ are rare. Although the obligate intracellular human pathogen Chlamydia trachomatis, the leading bacterial cause of sexually transmitted infections ... Read more >>

PLoS Pathog. (PLoS pathogens)
[2016, 12(8):e1005822]

Cited: 16 times

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Identification and Partial Characterization of Potential FtsL and FtsQ Homologs of Chlamydia.

Scot P Ouellette, Kelsey J Rueden, Yasser M AbdelRahman, John V Cox, Robert J Belland,

Chlamydia is amongst the rare bacteria that lack the critical cell division protein FtsZ. By annotation, Chlamydia also lacks several other essential cell division proteins including the FtsLBQ complex that links the early (e.g., FtsZ) and late (e.g., FtsI/Pbp3) components of the division machinery. Here, we report chlamydial FtsL and ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2015, 6:1264]

Cited: 6 times

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Chlamydia trachomatis utilizes the mammalian CLA1 lipid transporter to acquire host phosphatidylcholine essential for growth.

John V Cox, Yasser M Abdelrahman, Jan Peters, Nirun Naher, Robert J Belland,

Phosphatidylcholine is a constituent of Chlamydia trachomatis membranes that must be acquired from its mammalian host to support bacterial proliferation. The CLA1 (SR-B1) receptor is a bi-directional phosphatidylcholine/cholesterol transporter that is recruited to the inclusion of Chlamydia-infected cells along with ABCA1. C. trachomatis growth was inhibited in a dose-dependent manner ... Read more >>

Cell. Microbiol. (Cellular microbiology)
[2016, 18(3):305-318]

Cited: 6 times

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Aminomethyl spectinomycins as therapeutics for drug-resistant respiratory tract and sexually transmitted bacterial infections.

David F Bruhn, Samanthi L Waidyarachchi, Dora B Madhura, Dimitri Shcherbakov, Zhong Zheng, Jiuyu Liu, Yasser M Abdelrahman, Aman P Singh, Stefan Duscha, Chetan Rathi, Robin B Lee, Robert J Belland, Bernd Meibohm, Jason W Rosch, Erik C Böttger, Richard E Lee,

The antibiotic spectinomycin is a potent inhibitor of bacterial protein synthesis with a unique mechanism of action and an excellent safety index, but it lacks antibacterial activity against most clinically important pathogens. A series of N-benzyl-substituted 3'-(R)-3'-aminomethyl-3'-hydroxy spectinomycins was developed on the basis of a computational analysis of the aminomethyl ... Read more >>

Sci Transl Med (Science translational medicine)
[2015, 7(288):288ra75]

Cited: 8 times

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Infection with Chlamydia pneumoniae as a cause of coronary heart disease: the hypothesis is still untested.

J Thomas Grayston, Robert J Belland, Gerald I Byrne, Cho Chou Kuo, Julius Schachter, Walter E Stamm, Guangming Zhong,

Pathog Dis (Pathogens and disease)
[2015, 73(1):1-9]

Cited: 8 times

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Influence of the tryptophan-indole-IFNγ axis on human genital Chlamydia trachomatis infection: role of vaginal co-infections.

Ashok Aiyar, Alison J Quayle, Lyndsey R Buckner, Shardulendra P Sherchand, Theresa L Chang, Arnold H Zea, David H Martin, Robert J Belland,

The natural history of genital Chlamydia trachomatis infections can vary widely; infections can spontaneously resolve but can also last from months to years, potentially progressing to cause significant pathology. The host and bacterial factors underlying this wide variation are not completely understood, but emphasize the bacterium's capacity to evade/adapt to ... Read more >>

Front Cell Infect Microbiol (Frontiers in Cellular and Infection Microbiology)
[2014, 4:72]

Cited: 31 times

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Host HDL biogenesis machinery is recruited to the inclusion of Chlamydia trachomatis-infected cells and regulates chlamydial growth.

John V Cox, Nirun Naher, Yasser M Abdelrahman, Robert J Belland,

Chlamydia trachomatis is an obligate intracellular bacterial pathogen that is the most common cause of sexually transmitted bacterial infections and is the etiological agent of trachoma, the leading cause of preventable blindness. The organism infects epithelial cells of the genital tract and eyelid resulting in a damaging inflammatory response. Chlamydia ... Read more >>

Cell. Microbiol. (Cellular microbiology)
[2012, 14(10):1497-1512]

Cited: 30 times

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Plasmid-cured Chlamydia caviae activates TLR2-dependent signaling and retains virulence in the guinea pig model of genital tract infection.

Lauren C Frazer, Toni Darville, Kumar Chandra-Kuntal, Charles W Andrews, Matthew Zurenski, Margaret Mintus, Yasser M AbdelRahman, Robert J Belland, Robin R Ingalls, Catherine M O'Connell,

Loss of the conserved "cryptic" plasmid from C. trachomatis and C. muridarum is pleiotropic, resulting in reduced innate inflammatory activation via TLR2, glycogen accumulation and infectivity. The more genetically distant C. caviae GPIC is a natural pathogen of guinea pigs and induces upper genital tract pathology when inoculated intravaginally, modeling ... Read more >>

PLoS ONE (PloS one)
[2012, 7(1):e30747]

Cited: 19 times

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Toll-like receptor 2 activation by Chlamydia trachomatis is plasmid dependent, and plasmid-responsive chromosomal loci are coordinately regulated in response to glucose limitation by C. trachomatis but not by C. muridarum.

Catherine M O'Connell, Yasser M AbdelRahman, Erin Green, Hillary K Darville, Kazima Saira, Bennett Smith, Toni Darville, Amy M Scurlock, Christopher R Meyer, Robert J Belland,

We previously demonstrated that plasmid-deficient Chlamydia muridarum retains the ability to infect the murine genital tract but does not elicit oviduct pathology because it fails to activate Toll-like receptor 2 (TLR2). We derived a plasmid-cured derivative of the human genital isolate Chlamydia trachomatis D/UW-3/Cx, strain CTD153, which also fails to ... Read more >>

Infect. Immun. (Infection and immunity)
[2011, 79(3):1044-1056]

Cited: 58 times

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Developmental expression of non-coding RNAs in Chlamydia trachomatis during normal and persistent growth.

Yasser M Abdelrahman, Lorne A Rose, Robert J Belland,

Chlamydia trachomatis is an obligate intracellular bacterium that exhibits a unique biphasic developmental cycle that can be disrupted by growth in the presence of IFN-γ and β-lactams, giving rise to an abnormal growth state termed persistence. Here we have examined the expression of a family of non-coding RNAs (ncRNAs) that ... Read more >>

Nucleic Acids Res. (Nucleic acids research)
[2011, 39(5):1843-1854]

Cited: 19 times

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Proteome cataloging and relative quantification of Francisella tularensis tularensis strain Schu4 in 2D PAGE using preparative isoelectric focusing.

Robert H Waldo, Edwin D Cummings, Siva T Sarva, Janet M Brown, Crystal M Lauriano, Lorne A Rose, Robert J Belland, Karl E Klose, George M Hilliard,

The protein complement of whole cell extract of the bacterium Francisella tularensis tularensis was analyzed using two-dimensional electrophoresis with preparative isoelectric focusing in the first dimension. The format allows the quantification of relative protein abundance by linear densitometry and extends the potential dynamic range of protein detection by as much ... Read more >>

J. Proteome Res. (Journal of proteome research)
[2007, 6(9):3484-3490]

Cited: 4 times

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The p47 GTPases Iigp2 and Irgb10 regulate innate immunity and inflammation to murine Chlamydia psittaci infection.

Isao Miyairi, Venkat R R Arva Tatireddigari, Olaimatu S Mahdi, Lorne A Rose, Robert J Belland, Lu Lu, Robert W Williams, Gerald I Byrne,

C57BL/6J mice were 10(5)-fold more resistant to Chlamydia psittaci infection than DBA/2J mice by LD(100) determinations. Linkage analysis using BXD recombinant inbred strains revealed a single effector locus at a 1.5-Mbp region on chromosome 11 encoding a cluster of three p47 GTPases (Irgb10, Igtp, and Iigp2). Western blots of infected ... Read more >>

J. Immunol. (Journal of immunology (Baltimore, Md. : 1950))
[2007, 179(3):1814-1824]

Cited: 48 times

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Global transcriptional upregulation in the absence of increased translation in Chlamydia during IFNgamma-mediated host cell tryptophan starvation.

Scot P Ouellette, Thomas P Hatch, Yasser M AbdelRahman, Lorne A Rose, Robert J Belland, Gerald I Byrne,

The developmentally regulated intracellular pathogen Chlamydia pneumoniae is a natural tryptophan auxotroph. These organisms survive tryptophan starvation induced by host cell activation with IFNgamma by blocking maturation to the infectious form. In most bacteria, the stringent response is induced during amino acid starvation to promote survival. However, the response of ... Read more >>

Mol. Microbiol. (Molecular microbiology)
[2006, 62(5):1387-1401]

Cited: 51 times

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Different growth rates of Chlamydia trachomatis biovars reflect pathotype.

Isao Miyairi, Olaimatu S Mahdi, Scot P Ouellette, Robert J Belland, Gerald I Byrne,

Despite small genomic differences, Chlamydia trachomatis biovars exhibit diverse disease manifestations and different growth rates in vivo and in cell culture models.Chlamydial inclusion-forming units were enumerated over time in HeLa cells, to evaluate the length of the developmental cycle for C. trachomatis strains A, B, C, and E/Bour (ocular strains) ... Read more >>

J. Infect. Dis. (The Journal of infectious diseases)
[2006, 194(3):350-357]

Cited: 40 times

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The Chlamydia pneumoniae type III secretion-related lcrH gene clusters are developmentally expressed operons.

Scot P Ouellette, Yasser M Abdelrahman, Robert J Belland, Gerald I Byrne,

Two chlamydial homologues of the Yersinia lcrH chaperone for type III secretion system structural components are present within separate gene clusters. Quantitative transcriptional analyses demonstrated that each cluster is differentially regulated and expressed as an operon using major sigma factor elements, suggesting the presence of more elaborate developmental regulation mechanisms ... Read more >>

J. Bacteriol. (Journal of bacteriology)
[2005, 187(22):7853-7856]

Cited: 18 times

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Polymorphisms in the Chlamydia trachomatis cytotoxin locus associated with ocular and genital isolates.

John H Carlson, Scott Hughes, Daniel Hogan, Gordon Cieplak, Daniel E Sturdevant, Grant McClarty, Harlan D Caldwell, Robert J Belland,

Chlamydia trachomatis is a strict human pathogen producing infections that cause medically important chronic inflammatory diseases, such as blinding trachoma and tubal factor infertility. Isolates exist as serotypes that fall into distinct biologic and pathological groups corresponding to differences in infection tissue tropism and invasion properties. Paradoxically, genome sequencing of ... Read more >>

Infect. Immun. (Infection and immunity)
[2004, 72(12):7063-7072]

Cited: 68 times

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Genotypic differences in the Chlamydia pneumoniae tyrP locus related to vascular tropism and pathogenicity.

Jens Gieffers, Luke Durling, Scot P Ouellette, Jan Rupp, Matthias Maass, Gerald I Byrne, Harlan D Caldwell, Robert J Belland,

Chlamydia pneumoniae is an obligate intracellular pathogen that causes respiratory infections and has been associated with cardiovascular disease. We compared respiratory and cardiovascular isolates to find genetic differences associated with pathogenicity. A polymorphic region encoding a tyrosine/tryptophan permease was found to differ between disease isolates. Respiratory strains contained multiple copies ... Read more >>

J. Infect. Dis. (The Journal of infectious diseases)
[2003, 188(8):1085-1093]

Cited: 23 times

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Chlamydia pneumoniae and atherosclerosis.

Robert J Belland, Scot P Ouellette, Jens Gieffers, Gerald I Byrne,

Exposure to Chlamydia pneumoniae is extremely common, and respiratory infections occur repeatedly among most people. Strong associations exist between C. pneumoniae infection and atherosclerosis as demonstrated by: (i) sero-epidemiological studies showing that patients with cardiovascular disease have higher titres of anti-C. pneumoniae antibodies compared with control patients; (ii) detection of ... Read more >>

Cell. Microbiol. (Cellular microbiology)
[2004, 6(2):117-127]

Cited: 81 times

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Transcriptome analysis of chlamydial growth during IFN-gamma-mediated persistence and reactivation.

Robert J Belland, David E Nelson, Dezso Virok, Deborah D Crane, Daniel Hogan, Daniel Sturdevant, Wandy L Beatty, Harlan D Caldwell,

Chlamydia trachomatis is an obligatory intracellular prokaryotic parasite that causes a spectrum of clinically important chronic inflammatory diseases of humans. Persistent infection may play a role in the pathophysiology of chlamydial disease. Here we describe the chlamydial transcriptome in an in vitro model of IFN-gamma-mediated persistence and reactivation from persistence. ... Read more >>

Proc. Natl. Acad. Sci. U.S.A. (Proceedings of the National Academy of Sciences of the United States of America)
[2003, 100(26):15971-15976]

Cited: 120 times

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Micromanipulation of the Chlamydia pneumoniae inclusion: implications for cloning and host-pathogen interactions.

Jens Gieffers, Van Tamplin, Matthias Maass, Robert J Belland, Harlan D Caldwell,

The Chlamydia trachomatis inclusion is fragile, rendering it incompatible to micromanipulation. We show that the Chlamydia pneumoniae inclusion differs, being resistant to micromanipulation as shown by direct microinjection of the infected host cytosol or the inclusion itself. We have used micromanipulation to clone C. pneumoniae and to free it from ... Read more >>

FEMS Microbiol. Lett. (FEMS microbiology letters)
[2003, 226(1):45-49]

Cited: 2 times

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Carcinoembryonic antigen family receptor recognition by gonococcal Opa proteins requires distinct combinations of hypervariable Opa protein domains.

Martine P Bos, David Kao, Daniel M Hogan, Christopher C R Grant, Robert J Belland,

Neisserial Opa proteins function as a family of adhesins that bind heparan sulfate proteoglycan (HSPG) or carcinoembryonic antigen family (CEACAM) receptors on human host cells. In order to define the CEACAM binding domain on Opa proteins, we tested the binding properties of a series of gonococcal (strain MS11) recombinants producing ... Read more >>

Infect. Immun. (Infection and immunity)
[2002, 70(4):1715-1723]

Cited: 24 times

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Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase.

Christine Fehlner-Gardiner, Christine Roshick, John H Carlson, Scott Hughes, Robert J Belland, Harlan D Caldwell, Grant McClarty,

Here we report the cloning and sequencing of a region of the chlamydiae chromosome termed the "plasticity zone" from all the human serovars of C. trachomatis containing the tryptophan biosynthesis genes. Our results show that this region contains orthologues of the tryptophan repressor as well as the alpha and beta ... Read more >>

J. Biol. Chem. (The Journal of biological chemistry)
[2002, 277(30):26893-26903]

Cited: 109 times

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