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Occurrence, molecular characterization, and antimicrobial susceptibility of Yersinia enterocolitica isolated from retail food samples in China

Juan Wang, Ming Liu, Huixian Wang, Qingping Wu, Yu Ding, Tianxiang Xu, Guoxiang Ma, Yueming Zhong, Jumei Zhang, Moutong Chen, Liang Xue, Qinghua Ye, Haiyan Zeng, Xiaojuan Yang, Runshi Yang,

Yersinia enterocolitica (Y. enterocolitica) is an important foodborne pathogen that causes gastrointestinal syndromes. The objectives of this study were to examine the incidence of Y. enterocolitica strains in food samples in China and determine their genotypes, in addition to assessing their antimicrobial resistance and pathogenic potential. From June 2015 to ... Read more >>

Lebensm Wiss Technol (Lebensmittel-Wissenschaft + [i.e. und] Technologie.)
[2021, 150:Not Available]

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Protein hydrolysates from Pleurotus geesteranus obtained by simulated gastrointestinal digestion exhibit neuroprotective effects in H2 O2 -injured PC12 cells.

Shujian Wu, Mengfei Chen, Xiyu Liao, Rui Huang, Juan Wang, Yizhen Xie, Huiping Hu, Jumei Zhang, Qingping Wu, Yu Ding,

Neurodegenerative diseases are considered to be among the diseases most threatening to human beings. Increasing evidence shows that antioxidant hydrolysates/peptides with neuroprotective effects may relieve neurodegenerative diseases. However, related research in mushrooms, one of the richest sources of antioxidant hydrolysates/peptides, is in its infancy. Therefore, the in vitro neuroprotective effects ... Read more >>

J Food Biochem (Journal of food biochemistry)
[2021, :e13879]

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Effects of perioperative interventions for preventing postoperative delirium : A protocol for systematic review and meta-analysis of randomized controlled trials

Xia Li, Yanting Wang, Jie Liu, Yue Xiong, Shiqiang Chen, Jingjing Han, Wanli Xie, Qingping Wu,

Abstract <h4>Background:</h4> Postoperative delirium (POD) not only increases the medical burden but also adversely affects patient prognosis. Although some cases of delirium can be avoided by early intervention, there is no clear evidence indicating whether any of these measures can effectively prevent POD in specific patient groups. <h4>Objective:</h4> The aim ... Read more >>

Medicine (Baltimore) (Medicine)
[2021, 100(29):]

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First report of the optrA-carrying multidrug resistance genomic island in Campylobacter jejuni isolated from pigeon meat.

Shengjun Tang, Runshi Yang, Qingping Wu, Yu Ding, Zhi Wang, Jumei Zhang, Tao Lei, Shi Wu, Feng Zhang, Weipei Zhang, Liang Xue, Youxiong Zhang, Xianhu Wei, Rui Pang, Juan Wang,

Campylobacter spp., such as Campylobacter jejuni and Campylobacter coli, are important zoonotic Gram-negative pathogens that cause acute intestinal diseases in humans. The optrA gene, encoding an ATP-binding cassette F (ABC-F) protein that confers resistance to oxazolidinones and phenicols, has been found in C. coli in China. In this study, the ... Read more >>

Int J Food Microbiol (International journal of food microbiology)
[2021, 354:109320]

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Imported human norovirus in travelers, Shanghai port, China 2018: An epidemiological and whole genome sequencing study.

Zilong Zhang, Danlei Liu, Shenwei Li, Zilei Zhang, Jian Hou, Dapeng Wang, Qingping Wu, Yuan Jiang, Zhen'gan Tian,

<h4>Background</h4>Global mobility of the population has accelerated spread of the Human Norovirus (HuNoV), with long-distance travel in enclosed spaces increasing the opportunity for viral outbreaks. However, surveillance of HuNoV transmission is still lacking, especially in cross-border transportation.<h4>Method</h4>From 533 self-reported patients, 83 swab samples (15.6%) tested positive for HuNoV by RT-qPCR. ... Read more >>

Travel Med Infect Dis (Travel medicine and infectious disease)
[2021, 43:102140]

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Maximizing Magnesiation Capacity of Nanowire Cluster Oxides by Conductive Macromolecule Pillaring and Multication Intercalation.

Zhenguo Yao, Yifan Yu, Qingping Wu, Mengnan Cui, Xuejun Zhou, Jianjun Liu, Chilin Li,

Magnesium metal batteries (MMBs) have obtained the reputation owing to the high volumetric capacity, low reduction potential, and dendrite-free deposition behavior of the Mg metal anode. However, the bivalent nature of the Mg<sup>2+</sup> causes its strong coulombic interaction with the cathode host, which limits the reaction kinetics and reversibility of ... Read more >>

Small (Small (Weinheim an der Bergstrasse, Germany))
[2021, 17(30):e2102168]

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Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Xiong Gao, Jiayi Qi, Chi-Tang Ho, Bin Li, Yizhen Xie, Shaodan Chen, Huiping Hu, Zhongzheng Chen, Qingping Wu,

Two low-molecular-weight polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma leucocontextum fruiting bodies, and their physicochemical properties and antioxidant activities were investigated and compared in this study. The results showed that GLP-1 and GLP-2 were mainly composed of mannose, glucose, galactose, xylose, and arabinose, with weight-average molecular weights of 6.31 ... Read more >>

Antioxidants (Basel) (Antioxidants (Basel, Switzerland))
[2021, 10(7):]

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Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2.

Yuting Shang, Qifan Sun, Hanfang Chen, Qingping Wu, Moutong Chen, Shuanghong Yang, Mingzhu Du, Fei Zha, Qinghua Ye, Jumei Zhang,

<i>Salmonella</i> is a widely distributed foodborne pathogen. The use of <i>Salmonella</i> phages as biocontrol agents has recently gained significant interest. Because the <i>Salmonella</i> genus has high diversity, efforts are necessary to identify lytic <i>Salmonella</i> phages focusing on different serovars. Here, five <i>Salmonella</i> phages were isolated from soil samples, and vB_SalP_TR2 ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2021, 12:664810]

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Complete Genome Sequence of GII.9 Norovirus

Zilong Zhang, Danlei Liu, Zilei Zhang, Peng Tian, Qingping Wu, Dapeng Wang, Zhengan Tian,

Norovirus is recognized as one of the leading causes of acute gastroenteritis outbreaks. Genotype GII.9 was first detected in Norfolk, USA in 1997. However, the complete genome sequence of this genotype was not established yet. In this study, a complete genome sequence of a GII.9[P7] norovirus, marked as SCD1878, from ... Read more >>

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Quantitative detection of aflatoxin B1 using quantum dots-based immunoassay in a recyclable gravity-driven microfluidic chip.

Xinran Xiang, Qinghua Ye, Yuting Shang, Fan Li, Baoqing Zhou, Yanna Shao, Chufang Wang, Jumei Zhang, Liang Xue, Moutong Chen, Yu Ding, Qingping Wu,

To achieve rapid and sensitive detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>), we developed a polydimethylsiloxane gravity-driven cyclic microfluidic chip using the two-signal mode strategy. The structural design of the chip, together with the two-wavelength quantum dot ratio fluorescence, effectively eliminates the influence of environmental factors, improves the signal stability, and ensures ... Read more >>

Biosens Bioelectron (Biosensors & bioelectronics)
[2021, 190:113394]

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Roles of tolC on tolerance to bile salts and biofilm formation in Cronobacter malonaticus.

Xin Wang, Yaping Wang, Na Ling, Yizhong Shen, Danfeng Zhang, Dengyu Liu, Dexin Ou, Qingping Wu, Yingwang Ye,

Bile salts is one of essential components of bile secreted into the intestine to confer antibacterial protection. Cronobacter species are associated with necrotizing enterocolitis in newborns and show a strong tolerance to bile salts. However, little attempt has been made to focus on the molecular basis of the tolerance to ... Read more >>

J Dairy Sci (Journal of dairy science)
[2021, :]

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Bacterial community and composition of different traditional fermented dairy products in China, South Africa, and Sri Lanka by high-throughput sequencing of 16S rRNA genes

Tingting Liang, Xinqiang Xie, Jumei Zhang, Yu Ding, Qingping Wu,

The bacterial community and predictive functionality of 51 traditional fermented dairy products (raw milk, yoghurt, fermented milk curd, wrum, milk silk-skin, butter, milk granule, koumiss, and cheese) from three countries (China, Inner Mongolia and Xinjiang; South Africa; Sri Lanka) were investigated and compared by high-throughput sequencing of 16S rRNA genes. ... Read more >>

Lebensm Wiss Technol (Lebensmittel-Wissenschaft + [i.e. und] Technologie.)
[2021, 144:Not Available]

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PCR identification of Salmonella serovars for the E serogroup based on novel specific targets obtained by pan-genome analysis

Yuting Shang, Qinghua Ye, Qingping Wu, Rui Pang, Xinran Xiang, Chufang Wang, Fan Li, Baoqing Zhou, Liang Xue, Yinzhi Zhang, Xiulan Sun, Jumei Zhang,

Accurate serotyping of Salmonella is essential for epidemiological investigations and surveillance of Salmonella infections. Compared to traditional methods, polymerase chain reaction (PCR) is more sensitive and identifies Salmonella to the serotype level. Few genes have been used as a target for PCR serotyping; thus, research to identify specific targets of ... Read more >>

Lebensm Wiss Technol (Lebensmittel-Wissenschaft + [i.e. und] Technologie.)
[2021, 145:Not Available]

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Evaluation of the Cholesterol-Lowering Mechanism of Enterococcus faecium Strain 132 and Lactobacillus paracasei Strain 201 in Hypercholesterolemia Rats.

Lingshuang Yang, Xinqiang Xie, Ying Li, Lei Wu, Congcong Fan, Tingting Liang, Yu Xi, Shuanghong Yang, Haixin Li, Jumei Zhang, Yu Ding, Liang Xue, Moutong Chen, Juan Wang, Qingping Wu,

Hypercholesterolemia can cause many diseases, but it can effectively regulated by <i>Lactobacillus</i>. This study aimed to evaluate the cholesterol-lowering mechanism of <i>Enterococcus faecium</i> strain 132 and <i>Lactobacillus</i><i>paracasei</i> strain 201. These results showed that both the strains decreased serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), liver TC and ... Read more >>

Nutrients (Nutrients)
[2021, 13(6):]

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Prevalence, antibiotic susceptibility and genetic diversity of Campylobacter jejuni isolated from retail food in China

Juan Wang, Zhi Wang, Jumei Zhang, Yu Ding, Zonghao Ma, Fufeng Jiang, Xiang Nie, Shengjun Tang, Moutong Chen, Shi Wu, Haiyan Zeng, Tao Lei, Xiaojuan Yang, Shuhong Zhang, Qingping Wu,

Campylobacter jejuni is a common and important zoonotic pathogen that can cause bacterial gastroenteritis and other diseases, even leading to Guillain-Barré Syndrome or death. It is therefore necessary to investigate the prevalence and pathogenic risk of C. jejuni. Here, we investigated the prevalence of C. jejuni isolated from retail food ... Read more >>

Lebensm Wiss Technol (Lebensmittel-Wissenschaft + [i.e. und] Technologie.)
[2021, 143:Not Available]

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Novel phage vB_CtuP_B1 for controlling Cronobacter malonaticus and Cronobacter turicensis in ready-to-eat lettuce and powered infant formula

Haiyan Zeng, Chengsi Li, Dandan Luo, Jumei Zhang, Yu Ding, Moutong Chen, Xiaojuan Yang, Tao Lei, Shi Wu, Qinghua Ye, Rui Pang, Qihui Gu, Qingping Wu,

Cronobacter spp. are important foodborne pathogens that are a threat to people of all ages, but especially neonates and infants. Bacteriophages are biological agents that are potentially useful for the control of foodborne pathogens. However, there has been little research on the control of C. malonaticus and C. turicensis using ... Read more >>

Food Res Int (Food research international (Ottawa, Ont.))
[2021, 143:Not Available]

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Loop-mediated isothermal amplification (LAMP) for rapid detection of Salmonella in foods based on new molecular targets

Yuting Shang, Qinghua Ye, Shuzhen Cai, Qingping Wu, Rui Pang, Shuanghong Yang, Xinran Xiang, Chufang Wang, Fei Zha, Yu Ding, Yinzhi Zhang, Jiasheng Wang, Xiulan Sun, Jumei Zhang,

Various molecular techniques have been introduced for Salmonella detection. Loop-mediated isothermal amplification (LAMP) is a simple and easy-to-operate detection method compared with conventional PCR. However, the specificity and efficiency of DNA amplification relies on the selected target sequence. In this study, we aimed to identify new molecular targets and develop ... Read more >>

Lebensm Wiss Technol (Lebensmittel-Wissenschaft + [i.e. und] Technologie.)
[2021, 142:Not Available]

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Selective Isolation of Bifidobacterium From Human Faeces Using Pangenomics, Metagenomics, and Enzymology.

Shuanghong Yang, Xinqiang Xie, Jun Ma, Xingxiang He, Ying Li, Mingzhu Du, Longyan Li, Lingshuang Yang, Qingping Wu, Wei Chen, Jumei Zhang,

<i>Bifidobacterium</i>, an important genus for human health, is difficult to isolate. We applied metagenomics, pangenomics, and enzymology to determine the dominant glycoside hydrolase (GH) families of Bifidobacterium and designed selective medium for <i>Bifidobacterium</i> isolation. Pangenomics results showed that the GH13, GH3, GH42, and GH43 families were highly conserved in <i>Bifidobacterium</i>. ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2021, 12:649698]

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A Novel Gene vp0610 Negatively Regulates Biofilm Formation in Vibrio parahaemolyticus.

Fufeng Jiang, Tao Lei, Zhi Wang, Min He, Jumei Zhang, Juan Wang, Haiyan Zeng, Moutong Chen, Liang Xue, Qinghua Ye, Rui Pang, Shi Wu, Qihui Gu, Yu Ding, Qingping Wu,

<i>Vibrio parahaemolyticus</i> is an important foodborne pathogen and its biofilm formation ability facilitates its colonization and persistence in foods by protecting it from stresses including environmental variation and antibiotic exposure. Several important proteins are involved in biofilm formation; however, the identity and function of many remain unknown. In this study, ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2021, 12:656380]

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Mining and evaluating novel serovar-specific Salmonella C1 serogroup genes by polymerase chain reaction analysis

Qinghua Ye, Yuting Shang, Moutong Chen, Rui Pang, Fan Li, Xinran Xiang, Baoqing Zhou, Chufang Wang, Shuhong Zhang, Jumei Zhang, Juan Wang, Liang Xue, Yu Ding, Qingping Wu,

Salmonella is a food-borne pathogen that causes worldwide infections in humans and animals. Over 2600 Salmonella serovars have been identified, and serogroup C1 (O:6, 7 epitopes) contains highly diverse serovars associated with high pathogenicity. Here, we aimed to develop a reliable polymerase chain reaction (PCR) method for detecting serovar-specific markers ... Read more >>

Lebensm Wiss Technol (Lebensmittel-Wissenschaft + [i.e. und] Technologie.)
[2021, 141:Not Available]

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Incidence, toxin gene profiling, antimicrobial susceptibility, and genetic diversity of Bacillus cereus isolated from quick-frozen food in China

Hui Guo, Pengfei Yu, Shubo Yu, Juan Wang, Junhui Zhang, Ying Zhang, Xiyu Liao, Shi Wu, Qinghua Ye, Xiaojuan Yang, Tao Lei, Haiyan Zeng, Rui Pang, Jumei Zhang, Qingping Wu, Yu Ding,

Bacillus cereus is a well-known foodborne pathogen around the world and can be found in a variety of foods. However, few studies have been conducted to evaluate the risk of B. cereus in quick-frozen food in China. Hence, we investigated the prevalence, antimicrobial susceptibility, presence of toxin genes, biofilm formation ... Read more >>

Lebensm Wiss Technol (Lebensmittel-Wissenschaft + [i.e. und] Technologie.)
[2021, 140:Not Available]

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Transcriptional Dynamics of Genes Purportedly Involved in the Control of Meiosis, Carbohydrate, and Secondary Metabolism during Sporulation in Ganoderma lucidum.

Manjun Cai, Xiaowei Liang, Yuanchao Liu, Huiping Hu, Yizhen Xie, Shaodan Chen, Xiong Gao, Xiangmin Li, Chun Xiao, Diling Chen, Qingping Wu,

<i>Ganoderma lucidum</i> spores (GLS), the mature germ cells ejected from the abaxial side of the pileus, have diverse pharmacological effects. However, the genetic regulation of sporulation in this fungus remains unknown. Here, samples corresponding to the abaxial side of the pileus were collected from strain YW-1 at three sequential developmental ... Read more >>

Genes (Basel) (Genes)
[2021, 12(4):]

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An Investigation on the Occurrence and Molecular Characterization of Bacillus cereus in Meat and Meat Products in China.

Li Kong, Shubo Yu, Xiaoming Yuan, Chun Li, Pengfei Yu, Juan Wang, Hui Guo, Shi Wu, Qinghua Ye, Tao Lei, Xiaojuan Yang, Youxiong Zhang, Xianhu Wei, Haiyan Zeng, Jumei Zhang, Qingping Wu, Yu Ding,

<i>Bacillus cereus</i> is a common foodborne pathogen that can cause both gastrointestinal and nongastrointestinal diseases. In this study, we collected 603 meat and meat products from 39 major cities in China. The positive contamination rate of <i>B. cereus</i> in the collected samples was 26.37% (159/603), and the contamination level in ... Read more >>

Foodborne Pathog Dis (Foodborne pathogens and disease)
[2021, 18(5):306-314]

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Bacterial community and composition of soil in world’s longevity township Jiaoling, China by 16S rRNA high-throughput sequencing

Lei Wu, Xinqiang Xie, Jun Ma, Jumei Zhang, Yu Ding, Qingping Wu,

Healthy longevity is a complicated process, however, the underlying mechanisms between longevity and microbiota warrant investigation. To address this, we characterized a longevity trajectory of environmental microbiota in a longevity township. We used high-throughput sequencing of the 16S rRNA gene to analyse the composition and function of soil microbiota. The ... Read more >>

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Identification of Novel Sensitive and Reliable Serovar-Specific Targets for PCR Detection of Salmonella Serovars Hadar and Albany by Pan-Genome Analysis.

Qinghua Ye, Yuting Shang, Moutong Chen, Rui Pang, Fan Li, Xinran Xiang, Chufang Wang, Baoqing Zhou, Shuhong Zhang, Jumei Zhang, Xiaojuan Yang, Liang Xue, Yu Ding, Qingping Wu,

The accurate and rapid classification of <i>Salmonella</i> serovars is an essential focus for the identification of isolates involved in disease in humans and animals. The purpose of current research was to identify novel sensitive and reliable serovar-specific targets and to develop PCR method for <i>Salmonella</i> C2 serogroups (O:8 epitopes) in ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2021, 12:605984]

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