Full Text Journal Articles by
Author Paul Tempst


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Protein Biomarkers for Early Detection of Pancreatic Ductal Adenocarcinoma: Progress and Challenges.

Alex Root, Peter Allen, Paul Tempst, Kenneth Yu,

Approximately 75% of patients with pancreatic ductal adenocarcinoma are diagnosed with advanced cancer, which cannot be safely resected. The most commonly used biomarker CA19-9 has inadequate sensitivity and specificity for early detection, which we define as Stage I/II cancers. Therefore, progress in next-generation biomarkers is greatly needed. Recent reports have ... Read more >>

Cancers (Basel) (Cancers)
[2018, 10(3):]

Cited: 5 times

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Retraction Notice to: ATP Hydrolysis-Dependent Disassembly of the 26S Proteasome Is Part of the Catalytic Cycle.

Shalon E Babbitt, Alexi Kiss, Andrew E Deffenbaugh, Yie-Hwa Chang, Eric Bailly, Hediye Erdjument-Bromage, Paul Tempst, Tione Buranda, Larry A Sklar, Jennifer Baumler, Edward Gogol, Dorota Skowyra,

Cell (Cell)
[2018, 173(3):804]

Cited: 0 times

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Unique Transcriptional Programs Identify Subtypes of AKI.

Katherine Xu, Paul Rosenstiel, Neal Paragas, Christian Hinze, Xiaobo Gao, Tian Huai Shen, Max Werth, Catherine Forster, Rong Deng, Efrat Bruck, Roger W Boles, Alexandra Tornato, Tejashree Gopal, Madison Jones, Justin Konig, Jacob Stauber, Vivette D'Agati, Hediye Erdjument-Bromage, Subodh Saggi, Gebhard Wagener, Kai M Schmidt-Ott, Nicholas Tatonetti, Paul Tempst, Juan A Oliver, Paolo Guarnieri, Jonathan Barasch,

Two metrics, a rise in serum creatinine concentration and a decrease in urine output, are considered tantamount to the injury of the kidney tubule and the epithelial cells thereof (AKI). Yet neither criterion emphasizes the etiology or the pathogenetic heterogeneity of acute decreases in kidney excretory function. In fact, whether ... Read more >>

J Am Soc Nephrol (Journal of the American Society of Nephrology : JASN)
[2017, 28(6):1729-1740]

Cited: 38 times

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The histone variant MacroH2A1 regulates target gene expression in part by recruiting the transcriptional coregulator PELP1.

Kristine M Hussey, Hongshan Chen, Christine Yang, Eugene Park, Nasun Hah, Hediye Erdjument-Bromage, Paul Tempst, Matthew J Gamble, W Lee Kraus,

MacroH2A1 is a histone variant harboring an ∼25-kDa carboxyl-terminal macrodomain. Due to its enrichment on the inactive X chromosome, macroH2A1 was thought to play a role in transcriptional repression. However, recent studies have shown that macroH2A1 occupies autosomal chromatin and regulates genes in a context-specific manner. The macrodomain may play ... Read more >>

Mol Cell Biol (Molecular and cellular biology)
[2014, 34(13):2437-2449]

Cited: 14 times

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Aminopeptidase activities as prospective urinary biomarkers for bladder cancer.

Jennifer M Taylor, Mariana Yaneva, Kevin Velasco, John Philip, Hediye Erdjument-Bromage, Irina Ostrovnaya, Hans G Lilja, Bernard H Bochner, Paul Tempst,

<h4>Purpose</h4>Proteases have been implicated in cancer progression and invasiveness. We have investigated the activities, as opposed to simple protein levels, of selected aminopeptidases in urine specimens to serve as potential novel biomarkers for urothelial cancer.<h4>Experimental design</h4>The unique urinary proteomes of males and females were profiled to establish the presence of ... Read more >>

Proteomics Clin Appl (Proteomics. Clinical applications)
[2014, 8(5-6):317-326]

Cited: 6 times

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PRMT4 blocks myeloid differentiation by assembling a methyl-RUNX1-dependent repressor complex.

Ly P Vu, Fabiana Perna, Lan Wang, Francesca Voza, Maria E Figueroa, Paul Tempst, Hediye Erdjument-Bromage, Rui Gao, Sisi Chen, Elisabeth Paietta, Tony Deblasio, Ari Melnick, Yan Liu, Xinyang Zhao, Stephen D Nimer,

Defining the role of epigenetic regulators in hematopoiesis has become critically important, because recurrent mutations or aberrant expression of these genes has been identified in both myeloid and lymphoid hematological malignancies. We found that PRMT4, a type I arginine methyltransferase whose function in normal and malignant hematopoiesis is unknown, is ... Read more >>

Cell Rep (Cell reports)
[2013, 5(6):1625-1638]

Cited: 36 times

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Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress.

Marcus D Wilson, Michelle Harreman, Michael Taschner, Michael Taschner, James Reid, Jane Walker, Hediye Erdjument-Bromage, Paul Tempst, Jesper Q Svejstrup,

DNA damage triggers polyubiquitylation and degradation of the largest subunit of RNA polymerase II (RNAPII), a "mechanism of last resort" employed during transcription stress. In yeast, this process is dependent on Def1 through a previously unresolved mechanism. Here, we report that Def1 becomes activated through ubiquitylation- and proteasome-dependent processing. Def1 ... Read more >>

Cell (Cell)
[2013, 154(5):983-995]

Cited: 33 times

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Mass-encoded, synthetic biomarkers and multiplexed urinary monitoring: new frontiers in disease monitoring.

Paul Tempst,

Clin Chem (Clinical chemistry)
[2013, 59(12):1694-1695]

Cited: 0 times

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Monoubiquitination of filamin B regulates vascular endothelial growth factor-mediated trafficking of histone deacetylase 7.

Yu-Ting Su, Chengzhuo Gao, Yu Liu, Shuang Guo, Anthony Wang, Benlian Wang, Hediye Erdjument-Bromage, Masaru Miyagi, Paul Tempst, Hung-Ying Kao,

Nucleocytoplasmic shuttling of class IIa of histone deacetylases (HDACs) is a key mechanism that controls cell fate and animal development. We have identified the filamin B (FLNB) as a novel HDAC7-interacting protein that is required for temporal and spatial regulation of vascular endothelial growth factor (VEGF)-mediated HDAC7 cytoplasmic sequestration. This ... Read more >>

Mol Cell Biol (Molecular and cellular biology)
[2013, 33(8):1546-1560]

Cited: 15 times

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TRIM3, a tumor suppressor linked to regulation of p21(Waf1/Cip1.).

Y Liu, R Raheja, N Yeh, D Ciznadija, A M Pedraza, T Ozawa, E Hukkelhoven, H Erdjument-Bromage, P Tempst, N P Gauthier, C Brennan, E C Holland, A Koff,

The TRIM family of genes is largely studied because of their roles in development, differentiation and host cell antiviral defenses; however, roles in cancer biology are emerging. Loss of heterozygosity of the TRIM3 locus in ∼20% of human glioblastomas raised the possibility that this NHL-domain containing member of the TRIM ... Read more >>

Oncogene (Oncogene)
[2014, 33(3):308-315]

Cited: 24 times

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Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs.

Shruti Malu, Pablo De Ioannes, Mikhail Kozlov, Marsha Greene, Dailia Francis, Mary Hanna, Jesse Pena, Carlos R Escalante, Aya Kurosawa, Hediye Erdjument-Bromage, Paul Tempst, Noritaka Adachi, Paolo Vezzoni, Anna Villa, Aneel K Aggarwal, Patricia Cortes,

Artemis is an endonuclease that opens coding hairpin ends during V(D)J recombination and has critical roles in postirradiation cell survival. A direct role for the C-terminal region of Artemis in V(D)J recombination has not been defined, despite the presence of immunodeficiency and lymphoma development in patients with deletions in this ... Read more >>

J Exp Med (The Journal of experimental medicine)
[2012, 209(5):955-963]

Cited: 36 times

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NGAL (Lcn2) monomer is associated with tubulointerstitial damage in chronic kidney disease.

Thomas L Nickolas, Catherine S Forster, Meghan E Sise, Nicholas Barasch, David Solá-Del Valle, Melanie Viltard, Charles Buchen, Shlomo Kupferman, Maria Luisa Carnevali, Michael Bennett, Silvia Mattei, Achiropita Bovino, Lucia Argentiero, Andrea Magnano, Prasad Devarajan, Kiyoshi Mori, Hediye Erdjument-Bromage, Paul Tempst, Landino Allegri, Jonathan Barasch,

The type and the extent of tissue damage inform the prognosis of chronic kidney disease (CKD), but kidney biopsy is not a routine test. Urinary tests that correlate with specific histological findings might serve as surrogates for the kidney biopsy. We used immunoblots and ARCHITECT-NGAL assays to define the immunoreactivity ... Read more >>

Kidney Int (Kidney international)
[2012, 82(6):718-722]

Cited: 56 times

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LRPPRC is necessary for polyadenylation and coordination of translation of mitochondrial mRNAs.

Benedetta Ruzzenente, Metodi D Metodiev, Anna Wredenberg, Ana Bratic, Chan Bae Park, Yolanda Cámara, Dusanka Milenkovic, Volker Zickermann, Rolf Wibom, Kjell Hultenby, Hediye Erdjument-Bromage, Paul Tempst, Ulrich Brandt, James B Stewart, Claes M Gustafsson, Nils-Göran Larsson,

Regulation of mtDNA expression is critical for maintaining cellular energy homeostasis and may, in principle, occur at many different levels. The leucine-rich pentatricopeptide repeat containing (LRPPRC) protein regulates mitochondrial mRNA stability and an amino-acid substitution of this protein causes the French-Canadian type of Leigh syndrome (LSFC), a neurodegenerative disorder characterized ... Read more >>

EMBO J (The EMBO journal)
[2012, 31(2):443-456]

Cited: 155 times

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Merlin/NF2 functions upstream of the nuclear E3 ubiquitin ligase CRL4DCAF1 to suppress oncogenic gene expression.

Jonathan Cooper, Wei Li, Liru You, Gaia Schiavon, Angela Pepe-Caprio, Lu Zhou, Ryohei Ishii, Marco Giovannini, C Oliver Hanemann, Stephen B Long, Hediye Erdjument-Bromage, Pengbo Zhou, Paul Tempst, Filippo G Giancotti,

Integrin-mediated activation of PAK (p21-activated kinase) causes phosphorylation and inactivation of the FERM (4.1, ezrin, radixin, moesin) domain-containing protein Merlin, which is encoded by the NF2 (neurofibromatosis type 2) tumor suppressor gene. Conversely, cadherin engagement inactivates PAK, thus leading to accumulation of unphosphorylated Merlin. Current models imply that Merlin inhibits ... Read more >>

Sci Signal (Science signaling)
[2011, 4(188):pt6]

Cited: 16 times

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Superoxide dismutase 1 (SOD1) is a target for a small molecule identified in a screen for inhibitors of the growth of lung adenocarcinoma cell lines.

Romel Somwar, Hediye Erdjument-Bromage, Erik Larsson, David Shum, William W Lockwood, Guangli Yang, Chris Sander, Ouathek Ouerfelli, Paul J Tempst, Hakim Djaballah, Harold E Varmus,

We previously described four small molecules that reduced the growth of lung adenocarcinoma cell lines with either epidermal growth factor receptor (EGFR) or KRAS mutations in a high-throughout chemical screen. By combining affinity proteomics and gene expression analysis, we now propose superoxide dismutase 1 (SOD1) as the most likely target ... Read more >>

Proc Natl Acad Sci U S A (Proceedings of the National Academy of Sciences of the United States of America)
[2011, 108(39):16375-16380]

Cited: 59 times

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Bromodomain protein 7 interacts with PRMT5 and PRC2, and is involved in transcriptional repression of their target genes.

Sookil Tae, Vrajesh Karkhanis, Kevin Velasco, Mariana Yaneva, Hediye Erdjument-Bromage, Paul Tempst, Saïd Sif,

Histone modification regulates gene expression, and one major regulatory step in this process is the ability of proteins that recognize epigenetic marks to recruit enzymes required to specify transcriptional outcome. Here we show that BRD7 is a component of hSWI-SNF complexes that interacts with PRMT5 and PRC2. Recruitment studies revealed ... Read more >>

Nucleic Acids Res (Nucleic acids research)
[2011, 39(13):5424-5438]

Cited: 53 times

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MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome.

Yolanda Cámara, Jorge Asin-Cayuela, Chan Bae Park, Metodi D Metodiev, Yonghong Shi, Benedetta Ruzzenente, Christian Kukat, Bianca Habermann, Rolf Wibom, Kjell Hultenby, Thomas Franz, Hediye Erdjument-Bromage, Paul Tempst, B Martin Hallberg, Claes M Gustafsson, Nils-Göran Larsson,

Precise control of mitochondrial DNA gene expression is critical for regulation of oxidative phosphorylation capacity in mammals. The MTERF protein family plays a key role in this process, and its members have been implicated in regulation of transcription initiation and site-specific transcription termination. We now demonstrate that a member of ... Read more >>

Cell Metab (Cell metabolism)
[2011, 13(5):527-539]

Cited: 145 times

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L3MBTL2 protein acts in concert with PcG protein-mediated monoubiquitination of H2A to establish a repressive chromatin structure.

Patrick Trojer, Alina R Cao, Zhonghua Gao, Yan Li, Jin Zhang, Xiaoqin Xu, Guohong Li, Regine Losson, Hediye Erdjument-Bromage, Paul Tempst, Peggy J Farnham, Danny Reinberg,

We have identified human MBT domain-containing protein L3MBTL2 as an integral component of a protein complex that we termed Polycomb repressive complex 1 (PRC1)-like 4 (PRC1L4), given the copresence of PcG proteins RING1, RING2, and PCGF6/MBLR. PRC1L4 also contained E2F6 and CBX3/HP1γ, known to function in transcriptional repression. PRC1L4-mediated repression ... Read more >>

Mol Cell (Molecular cell)
[2011, 42(4):438-450]

Cited: 84 times

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PRC2 complexes with JARID2, MTF2, and esPRC2p48 in ES cells to modulate ES cell pluripotency and somatic cell reprogramming.

Zhuo Zhang, Amanda Jones, Chiao-Wang Sun, Chao Li, Chia-Wei Chang, Heui-Yun Joo, Qian Dai, Matthew R Mysliwiec, Li-Chen Wu, Yahong Guo, Wei Yang, Kaimao Liu, Kevin M Pawlik, Hediye Erdjument-Bromage, Paul Tempst, Youngsook Lee, Jinrong Min, Tim M Townes, Hengbin Wang,

Polycomb repressive complex two (PRC2) has been implicated in embryonic stem (ES) cell pluripotency; however, the mechanistic roles of this complex are unclear. It was assumed that ES cells contain PRC2 with the same subunit composition as that identified in HeLa cells and Drosophila embryos. Here, we report that PRC2 ... Read more >>

Stem Cells (Stem cells (Dayton, Ohio))
[2011, 29(2):229-240]

Cited: 91 times

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SETDB1 is involved in postembryonic DNA methylation and gene silencing in Drosophila.

Dawei Gou, Monica Rubalcava, Silvia Sauer, Felipe Mora-Bermúdez, Hediye Erdjument-Bromage, Paul Tempst, Elisabeth Kremmer, Frank Sauer,

DNA methylation is fundamental for the stability and activity of genomes. Drosophila melanogaster and vertebrates establish a global DNA methylation pattern of their genome during early embryogenesis. Large-scale analyses of DNA methylation patterns have uncovered revealed that DNA methylation patterns are dynamic rather than static and change in a gene-specific ... Read more >>

PLoS One (PloS one)
[2010, 5(5):e10581]

Cited: 15 times

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Protein-based multiplex assays: mock presubmissions to the US Food and Drug Administration.

Fred E Regnier, Steven J Skates, Mehdi Mesri, Henry Rodriguez, Zivana Tezak, Marina V Kondratovich, Michail A Alterman, Joshua D Levin, Donna Roscoe, Eugene Reilly, James Callaghan, Kellie Kelm, David Brown, Reena Philip, Steven A Carr, Daniel C Liebler, Susan J Fisher, Paul Tempst, Tara Hiltke, Larry G Kessler, Christopher R Kinsinger, David F Ransohoff, Elizabeth Mansfield, N Leigh Anderson,

As a part of ongoing efforts of the NCI-FDA Interagency Oncology Task Force subcommittee on molecular diagnostics, members of the Clinical Proteomic Technology Assessment for Cancer program of the National Cancer Institute have submitted 2 protein-based multiplex assay descriptions to the Office of In Vitro Diagnostic Device Evaluation and Safety, ... Read more >>

Clin Chem (Clinical chemistry)
[2010, 56(2):165-171]

Cited: 36 times

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Super-SILAC for tumors and tissues.

Thomas A Neubert, Paul Tempst,

Nat Methods (Nature methods)
[2010, 7(5):361-362]

Cited: 12 times

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Analytical validation of protein-based multiplex assays: a workshop report by the NCI-FDA interagency oncology task force on molecular diagnostics.

Henry Rodriguez, Zivana Tezak, Mehdi Mesri, Steven A Carr, Daniel C Liebler, Susan J Fisher, Paul Tempst, Tara Hiltke, Larry G Kessler, Christopher R Kinsinger, Reena Philip, David F Ransohoff, Steven J Skates, Fred E Regnier, N Leigh Anderson, Elizabeth Mansfield, ,

Clinical proteomics has the potential to enable the early detection of cancer through the development of multiplex assays that can inform clinical decisions. However, there has been some uncertainty among translational researchers and developers as to the specific analytical measurement criteria needed to validate protein-based multiplex assays. To begin to ... Read more >>

Clin Chem (Clinical chemistry)
[2010, 56(2):237-243]

Cited: 32 times

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Interlaboratory study characterizing a yeast performance standard for benchmarking LC-MS platform performance.

Amanda G Paulovich, Dean Billheimer, Amy-Joan L Ham, Lorenzo Vega-Montoto, Paul A Rudnick, David L Tabb, Pei Wang, Ronald K Blackman, David M Bunk, Helene L Cardasis, Karl R Clauser, Christopher R Kinsinger, Birgit Schilling, Tony J Tegeler, Asokan Mulayath Variyath, Mu Wang, Jeffrey R Whiteaker, Lisa J Zimmerman, David Fenyo, Steven A Carr, Susan J Fisher, Bradford W Gibson, Mehdi Mesri, Thomas A Neubert, Fred E Regnier, Henry Rodriguez, Cliff Spiegelman, Stephen E Stein, Paul Tempst, Daniel C Liebler,

Optimal performance of LC-MS/MS platforms is critical to generating high quality proteomics data. Although individual laboratories have developed quality control samples, there is no widely available performance standard of biological complexity (and associated reference data sets) for benchmarking of platform performance for analysis of complex biological proteomes across different laboratories ... Read more >>

Mol Cell Proteomics (Molecular & cellular proteomics : MCP)
[2010, 9(2):242-254]

Cited: 98 times

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Repeatability and reproducibility in proteomic identifications by liquid chromatography-tandem mass spectrometry.

David L Tabb, Lorenzo Vega-Montoto, Paul A Rudnick, Asokan Mulayath Variyath, Amy-Joan L Ham, David M Bunk, Lisa E Kilpatrick, Dean D Billheimer, Ronald K Blackman, Helene L Cardasis, Steven A Carr, Karl R Clauser, Jacob D Jaffe, Kevin A Kowalski, Thomas A Neubert, Fred E Regnier, Birgit Schilling, Tony J Tegeler, Mu Wang, Pei Wang, Jeffrey R Whiteaker, Lisa J Zimmerman, Susan J Fisher, Bradford W Gibson, Christopher R Kinsinger, Mehdi Mesri, Henry Rodriguez, Stephen E Stein, Paul Tempst, Amanda G Paulovich, Daniel C Liebler, Cliff Spiegelman,

The complexity of proteomic instrumentation for LC-MS/MS introduces many possible sources of variability. Data-dependent sampling of peptides constitutes a stochastic element at the heart of discovery proteomics. Although this variation impacts the identification of peptides, proteomic identifications are far from completely random. In this study, we analyzed interlaboratory data sets ... Read more >>

J Proteome Res (Journal of proteome research)
[2010, 9(2):761-776]

Cited: 258 times

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