Full Text Journal Articles by
Author Parbir S Grewal

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Peroxisome compartmentalization of a toxic enzyme improves alkaloid production.

Parbir S Grewal, Jennifer A Samson, Jordan J Baker, Brian Choi, John E Dueber,

Eukaryotic cells compartmentalize metabolic pathways in organelles to achieve optimal reaction conditions and avoid crosstalk with cytosolic factors. We found that cytosolic expression of norcoclaurine synthase (NCS), the enzyme that catalyzes the first committed reaction in benzylisoquinoline alkaloid biosynthesis, is toxic in Saccharomyces cerevisiae and, consequently, restricts (S)-reticuline production. We ... Read more >>

Nat Chem Biol (Nature chemical biology)
[2020, :]

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A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids.

Michael E Pyne, Kaspar Kevvai, Parbir S Grewal, Lauren Narcross, Brian Choi, Leanne Bourgeois, John E Dueber, Vincent J J Martin,

The tetrahydroisoquinoline (THIQ) moiety is a privileged substructure of many bioactive natural products and semi-synthetic analogs. Plants manufacture more than 3,000 THIQ alkaloids, including the opioids morphine and codeine. While microbial species have been engineered to synthesize a few compounds from the benzylisoquinoline alkaloid (BIA) family of THIQs, low product ... Read more >>

Nat Commun (Nature communications)
[2020, 11(1):3337]

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Investigation of Metal-Catalyzed Antibody Carbonylation With an Improved Protein Carbonylation Assay.

Yi Yang, Anna Mah, Inn H Yuk, Parbir S Grewal, Abigail Pynn, Will Cole, Di Gao, Fan Zhang, Jia Chen, Lynn Gennaro, Christian Schöneich,

Protein carbonylation is a posttranslational modification referring to the occurrence of aldehydes and ketones in proteins. The current understanding of how carbonylation, in particular, metal-catalyzed carbonylation, occurs in recombinant mAbs during production and storage is very limited. To facilitate investigations into mAb carbonylation, we developed a protein carbonylation assay with ... Read more >>

J Pharm Sci (Journal of pharmaceutical sciences)
[2018, 107(10):2570-2580]

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Short- and long-term effects on mAb-producing CHO cell lines after cryopreservation.

Jayashree Subramanian, Rigzen P S Aulakh, Parbir S Grewal, Mark Sanford, Abigail F J Pynn, Inn H Yuk,

Cryopreservation provides the foundation for research, development, and manufacturing operations in the CHO-based biopharmaceutical industry. Despite its criticality, studies are lacking that explicitly demonstrate that the routine cell banking process and the potential stress and damage during cryopreservation and recovery from thaw have no lasting detrimental effects on CHO cells. ... Read more >>

Biotechnol. Prog. (Biotechnology progress)
[2018, 34(2):463-477]

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Bioproduction of a betalain color palette in Saccharomyces cerevisiae.

Parbir S Grewal, Cyrus Modavi, Zachary N Russ, Nicholas C Harris, John E Dueber,

Betalains are a family of natural pigments found exclusively in the plant order Caryophyllales. All members of this chemical family are biosynthesized through the common intermediate betalamic acid, which is capable of spontaneously condensing with various primary and secondary amines to produce betalains. Of particular interest is the red-violet betanin, ... Read more >>

Metab Eng (Metabolic engineering)
[2018, 45:180-188]

Cited: 11 times

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