Full Text Journal Articles by
Author Nicole Pfarr

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Morphology Matters: A Critical Reappraisal of the Clinical Relevance of Morphologic Criteria From the 2019 WHO Classification in a Large Colorectal Cancer Cohort Comprising 1004 Cases.

Moritz Jesinghaus, Maxime Schmitt, Corinna Lang, Marianne Reiser, Alexander Scheiter, Björn Konukiewitz, Katja Steiger, Miguel Silva, Markus Tschurtschenthaler, Sebastian Lange, Sebastian Foersch, Karl F Becker, Dieter Saur, Helmut Friess, Kathrin Halfter, Jutta Engel, Melanie Boxberg, Nicole Pfarr, Dirk Wilhelm, Wilko Weichert,

The 2019 World Health Organization (WHO) classification of colorectal carcinoma (CRC) profoundly reclassified CRC subtypes and introduces tumor budding as a second major grading criterion, while condensing conventional grade into a 2-tiered system. So far it remains largely unexplored how these parameters interact with each other and whether they truly ... Read more >>

Am J Surg Pathol (The American journal of surgical pathology)
[2021, 45(7):969-978]

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Validation of a Targeted Next-Generation Sequencing Panel for Tumor Mutation Burden Analysis: Results from the Onconetwork Immuno-Oncology Consortium.

Francesca Fenizia, Ilaria Alborelli, Jose Luis Costa, Claudia Vollbrecht, Beatriz Bellosillo, Winand Dinjens, Volker Endris, Carina Heydt, Katharina Leonards, Sabine Merkelback-Bruse, Nicole Pfarr, Ronald van Marion, Christopher Allen, Ruchi Chaudhary, Rajesh Gottimukkala, Fiona Hyland, Elaine Wong-Ho, Philip Jermann, Jose Carlos Machado, Michael Hummel, Albrecht Stenzinger, Nicola Normanno,

Tumor mutation burden (TMB) is evaluated as a biomarker of response to immunotherapy. We present the efforts of the Onconetwork Immuno-Oncology Consortium to validate a commercial targeted sequencing test for TMB calculation. A three-phase study was designed to validate the Oncomine Tumor Mutational Load (OTML) assay at nine European laboratories. ... Read more >>

J Mol Diagn (The Journal of molecular diagnostics : JMD)
[2021, :]

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Therapy response and prognosis of patients with early breast cancer with low positivity for hormone receptors - An analysis of 2765 patients from neoadjuvant clinical trials.

Sonia L Villegas, Valentina Nekljudova, Nicole Pfarr, Jutta Engel, Michael Untch, Simone Schrodi, Frank Holms, Hans U Ulmer, Peter A Fasching, Karsten E Weber, Christian Albig, Clemens Heinrichs, Frederik Marmé, Arndt Hartmann, Claus Hanusch, Wolfgang D Schmitt, Jens Huober, Bianca Lederer, Marion van Mackelenbergh, Hans Tesch, Christian Jackisch, Mahdi Rezai, Peter Sinn, Bruno V Sinn, John Hackmann, Marion Kiechle, Andreas Schneeweiss, Wilko Weichert, Carsten Denkert, Sibylle Loibl,

<h4>Aim</h4>To evaluate HER2-negative breast cancer (BC) with a low hormone receptor (HR) expression, with regard to pathological complete response (pCR) and survival, in comparison to triple-negative BC (TNBC) and strong HR-positive BC.<h4>Methods</h4>We compared negative [oestrogen (ER) and progesterone receptor (PR) <1%], low-positive (ER and/or PR 1-9%) and strong-positive (ER or PR ... Read more >>

Eur J Cancer (European journal of cancer (Oxford, England : 1990))
[2021, 148:159-170]

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PALLD mutation in a European family conveys a stromal predisposition for familial pancreatic cancer.

Lucia Liotta, Sebastian Lange, H Carlo Maurer, Kenneth P Olive, Rickmer Braren, Nicole Pfarr, Sebastian Burger, Alexander Muckenhuber, Moritz Jesinghaus, Katja Steiger, Wilko Weichert, Helmut Friess, Roland Schmid, Hana Algül, Philipp J Jost, Juliane Ramser, Christine Fischer, Anne S Quante, Maximilian Reichert, Michael Quante,

BACKGROUNDPancreatic cancer is one of the deadliest cancers, with low long-term survival rates. Despite recent advances in treatment, it is important to identify and screen high-risk individuals for cancer prevention. Familial pancreatic cancer (FPC) accounts for 4%-10% of pancreatic cancers. Several germline mutations are related to an increased risk and ... Read more >>

JCI Insight (JCI insight)
[2021, 6(8):]

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Detection of gene fusions using targeted next-generation sequencing: a comparative evaluation.

Carina Heydt, Christina B Wölwer, Oscar Velazquez Camacho, Svenja Wagener-Ryczek, Roberto Pappesch, Janna Siemanowski, Jan Rehker, Florian Haller, Abbas Agaimy, Karl Worm, Thomas Herold, Nicole Pfarr, Wilko Weichert, Thomas Kirchner, Andreas Jung, Jörg Kumbrink, Wolfgang Goering, Irene Esposito, Reinhard Buettner, Axel M Hillmer, Sabine Merkelbach-Bruse,

<h4>Background</h4>Gene fusions represent promising targets for cancer therapy in lung cancer. Reliable detection of multiple gene fusions is therefore essential.<h4>Methods</h4>Five commercially available parallel sequencing assays were evaluated for their ability to detect gene fusions in eight cell lines and 18 FFPE tissue samples carrying a variety of known gene fusions. ... Read more >>

BMC Med Genomics (BMC medical genomics)
[2021, 14(1):62]

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TP53 germline mutations in the context of families with hereditary breast and ovarian cancer: a clinical challenge.

Sabine Grill, Juliane Ramser, Heide Hellebrand, Nicole Pfarr, Melanie Boxberg, Christine Brambs, Nina Ditsch, Alfons Meindl, Eva Groß, Thomas Meitinger, Marion Kiechle, Anne S Quante,

<h4>Purpose</h4>TP53germline (g) mutations, associated with the Li-Fraumeni syndrome (LFS), have rarely been reported in the context of hereditary breast and ovarian cancer (HBOC). The prevalence and cancer risks in this target group are unknown and counseling remains challenging. Notably an extensive high-risk surveillance program is implemented, which evokes substantial psychological ... Read more >>

Arch Gynecol Obstet (Archives of gynecology and obstetrics)
[2021, 303(6):1557-1567]

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The immunologic tumor microenvironment in endometrioid endometrial cancer in the morphomolecular context: mutual correlations and prognostic impact depending on molecular alterations.

Barbara Willvonseder, Fabian Stögbauer, Katja Steiger, Moritz Jesinghaus, Peer-Hendrik Kuhn, Christine Brambs, Jutta Engel, Holger Bronger, Georg Philipp Schmidt, Bernhard Haller, Wilko Weichert, Gisela Keller, Aurelia Noske, Nicole Pfarr, Melanie Boxberg,

<h4>Objective</h4>POLE-mutant, microsatellite-instable (MSI), p53-mutant and non-specific molecular profile (NSMP) are TCGA-defined molecular subgroups of endometrial cancer (EC). Hypothesizing that morphology and tumor immunology might differ depending on molecular background concerning composition and prognostic impact, we aimed to comprehensively interconnect morphologic, immunologic and molecular data.<h4>Methods</h4>TCGA-defined molecular groups were determined by immunohistochemistry ... Read more >>

Cancer Immunol Immunother (Cancer immunology, immunotherapy : CII)
[2021, 70(6):1679-1689]

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Loss of RNF43 Function Contributes to Gastric Carcinogenesis by Impairing DNA Damage Response.

Victoria Neumeyer, Anna Brutau-Abia, Michael Allgäuer, Nicole Pfarr, Wilko Weichert, Christina Falkeis-Veits, Elisabeth Kremmer, Michael Vieth, Markus Gerhard, Raquel Mejías-Luque,

<h4>Background & aims</h4>RING finger protein 43 (RNF43) is a tumor suppressor that frequently is mutated in gastric tumors. The link between RNF43 and modulation of Wingless-related integration site (WNT) signaling has not been shown clearly in the stomach. Because mutations in RNF43 are highly enriched in microsatellite-unstable gastric tumors, which ... Read more >>

Cell Mol Gastroenterol Hepatol (Cellular and molecular gastroenterology and hepatology)
[2021, 11(4):1071-1094]

Cited: 2 times

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Next-generation diagnostics for precision oncology: Preanalytical considerations, technical challenges, and available technologies.

Wencke Walter, Nicole Pfarr, Manja Meggendorfer, Philipp Jost, Torsten Haferlach, Wilko Weichert,

Molecular diagnostics as the centrepiece of precision oncology has gone through revolutionary developments over the last decade, becoming tremendously broad, deep and precise with still ongoing advancements. In the majority of scenarios, treatment selection for cancer patients without any type of molecular characterization is no longer conceivable. Considering the impact ... Read more >>

Semin Cancer Biol (Seminars in cancer biology)
[2020, :]

Cited: 1 time

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Aggressive PDACs Show Hypomethylation of Repetitive Elements and the Execution of an Intrinsic IFN Program Linked to a Ductal Cell of Origin.

Elisa Espinet, Zuguang Gu, Charles D Imbusch, Nathalia A Giese, Magdalena Büscher, Mariam Safavi, Silke Weisenburger, Corinna Klein, Vanessa Vogel, Mattia Falcone, Jacob Insua-Rodríguez, Manuel Reitberger, Vera Thiel, Steffi O Kossi, Alexander Muckenhuber, Karnjit Sarai, Alex Y L Lee, Elyne Backx, Soheila Zarei, Matthias M Gaida, Manuel Rodríguez-Paredes, Elisa Donato, Hsi-Yu Yen, Roland Eils, Matthias Schlesner, Nicole Pfarr, Thilo Hackert, Christoph Plass, Benedikt Brors, Katja Steiger, Dieter Weichenhan, H Efsun Arda, Ilse Rooman, Janel L Kopp, Oliver Strobel, Wilko Weichert, Martin R Sprick, Andreas Trumpp,

Pancreatic ductal adenocarcinoma (PDAC) is characterized by extensive desmoplasia, which challenges the molecular analyses of bulk tumor samples. Here we FACS-purified epithelial cells from human PDAC and normal pancreas and derived their genome-wide transcriptome and DNA methylome landscapes. Clustering based on DNA methylation revealed two distinct PDAC groups displaying different ... Read more >>

Cancer Discov (Cancer discovery)
[2021, 11(3):638-659]

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Immunohistochemically Characterized Intratumoral Heterogeneity Is a Prognostic Marker in Human Glioblastoma.

Friederike Liesche-Starnecker, Karoline Mayer, Florian Kofler, Sandra Baur, Friederike Schmidt-Graf, Johanna Kempter, Georg Prokop, Nicole Pfarr, Wu Wei, Jens Gempt, Stephanie E Combs, Claus Zimmer, Bernhard Meyer, Benedikt Wiestler, Jürgen Schlegel,

Tumor heterogeneity is considered to be a hallmark of glioblastoma (GBM). Only more recently, it has become apparent that GBM is not only heterogeneous between patients (intertumoral heterogeneity) but more importantly, also within individual patients (intratumoral heterogeneity). In this study, we focused on assessing intratumoral heterogeneity. For this purpose, the ... Read more >>

Cancers (Basel) (Cancers)
[2020, 12(10):]

Cited: 1 time

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MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically.

Enkhtsetseg Munkhbaatar, Michelle Dietzen, Deepti Agrawal, Martina Anton, Moritz Jesinghaus, Melanie Boxberg, Nicole Pfarr, Pidassa Bidola, Sebastian Uhrig, Ulrike Höckendorf, Anna-Lena Meinhardt, Adam Wahida, Irina Heid, Rickmer Braren, Ritu Mishra, Arne Warth, Thomas Muley, Patrina S P Poh, Xin Wang, Stefan Fröhling, Katja Steiger, Julia Slotta-Huspenina, Martijn van Griensven, Franz Pfeiffer, Sebastian Lange, Roland Rad, Magda Spella, Georgios T Stathopoulos, Jürgen Ruland, Florian Bassermann, Wilko Weichert, Andreas Strasser, Caterina Branca, Mathias Heikenwalder, Charles Swanton, Nicholas McGranahan, Philipp J Jost,

Evasion of programmed cell death represents a critical form of oncogene addiction in cancer cells. Understanding the molecular mechanisms underpinning cancer cell survival despite the oncogenic stress could provide a molecular basis for potential therapeutic interventions. Here we explore the role of pro-survival genes in cancer cell integrity during clonal ... Read more >>

Nat Commun (Nature communications)
[2020, 11(1):4527]

Cited: 2 times

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Implementing cell-free DNA of pancreatic cancer patient-derived organoids for personalized oncology.

Zahra Dantes, Hsi-Yu Yen, Nicole Pfarr, Christof Winter, Katja Steiger, Alexander Muckenhuber, Alexander Hennig, Sebastian Lange, Thomas Engleitner, Rupert Öllinger, Roman Maresch, Felix Orben, Irina Heid, Georgios Kaissis, Kuangyu Shi, Geoffrey Topping, Fabian Stögbauer, Matthias Wirth, Katja Peschke, Aristeidis Papargyriou, Massoud Rezaee-Oghazi, Karin Feldmann, Arlett Pg Schäfer, Raphela Ranjan, Clara Lubeseder-Martellato, Daniel E Stange, Thilo Welsch, Marc Martignoni, Güralp O Ceyhan, Helmut Friess, Alexander Herner, Lucia Liotta, Matthias Treiber, Guido von Figura, Mohamed Abdelhafez, Peter Klare, Christoph Schlag, Hana Algül, Jens Siveke, Rickmer Braren, Gregor Weirich, Wilko Weichert, Dieter Saur, Roland Rad, Roland M Schmid, Günter Schneider, Maximilian Reichert,

One of the major challenges in using pancreatic cancer patient-derived organoids (PDOs) in precision oncology is the time from biopsy to functional characterization. This is particularly true for endoscopic ultrasound-guided fine-needle aspiration biopsies, typically resulting in specimens with limited tumor cell yield. Here, we tested conditioned media of individual PDOs ... Read more >>

JCI Insight (JCI insight)
[2020, 5(15):]

Cited: 3 times

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Impact of Tumor Localization and Molecular Subtypes on the Prognostic and Predictive Significance of p53 Expression in Gastric Cancer.

Bianca Grosser, Meike Kohlruss, Julia Slotta-Huspenina, Moritz Jesinghaus, Nicole Pfarr, Katja Steiger, Alexander Novotny, Matthias M Gaida, Thomas Schmidt, Alexander Hapfelmeier, Katja Ott, Wilko Weichert, Gisela Keller,

We investigated the prognostic and predictive impact of p53 expression for gastric cancer (GC) patients treated without or with preoperative chemotherapy (CTx) and its relationship with specific molecular GC subtypes. Specimens from 694 GC patients (562 surgical resection specimens without or after CTx, 132 biopsies before CTx) were analyzed by ... Read more >>

Cancers (Basel) (Cancers)
[2020, 12(6):]

Cited: 2 times

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NTRK testing: First results of the QuiP-EQA scheme and a comprehensive map of NTRK fusion variants and their diagnostic coverage by targeted RNA-based NGS assays.

Martina Kirchner, Julia Glade, Ulrich Lehmann, Sabine Merkelbach-Bruse, Michael Hummel, Annika Lehmann, Marcel Trautmann, Jörg Kumbrink, Andreas Jung, Wolfgang Dietmaier, Volker Endris, Daniel Kazdal, Carolin Ploeger, Matthias Evert, David Horst, Hans Kreipe, Thomas Kirchner, Eva Wardelmann, Reinhard Büttner, Wilko Weichert, Manfred Dietel, Peter Schirmacher, Albrecht Stenzinger, Nicole Pfarr,

Gene fusions involving the three neurotrophic tyrosine receptor kinase genes NTRK1, NTRK2, or NTRK3 were identified as oncogenic drivers in many cancer types. Two small molecule inhibitors have been tested in clinical trials recently and require the detection of a NTRK fusion gene prior to therapeutic application. Fluorescence in situ ... Read more >>

Genes Chromosomes Cancer (Genes, chromosomes & cancer)
[2020, 59(8):445-453]

Cited: 4 times

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Maintenance of epithelial traits and resistance to mesenchymal reprogramming promote proliferation in metastatic breast cancer

Laura Eichelberger, Massimo Saini, Helena Domínguez Moreno, Corinna Klein, Johanna Bartsch, Mattia Falcone, Manuel Reitberger, Elisa Espinet, Vanessa Vogel, Elisabeth Graf, Thomas Schwarzmayr, Tim-Matthias Strom, Mareike Lehmann, Melanie Königshoff, Nicole Pfarr, Roberto Würth, Elisa Donato, Simon Haas, Saskia Spaich, Marc Sütterlin, Andreas Schneeweiss, Wilko Weichert, Gunnar Schotta, Andreas Trumpp, Martin Sprick, Christina Scheel,

Despite important advances in the treatment of breast cancer, the 5-year survival rate for patients with distant metastasis remains less than 30%. Metastasis is a complex, multi-step process beginning with local invasion and ending with the outgrowth of systemically disseminated cells into actively proliferating metastases that ultimately cause the destruction ... Read more >>

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Evaluation of a Hybrid Capture-Based Pan-Cancer Panel for Analysis of Treatment Stratifying Oncogenic Aberrations and Processes.

Leonie I Kroeze, Richarda M de Voer, Eveline J Kamping, Daniel von Rhein, Erik A M Jansen, Mandy J W Hermsen, Massimo C P Barberis, Johan Botling, Eva M Garrido-Martin, Florian Haller, Ludovic Lacroix, Brigitte Maes, Sabine Merkelbach-Bruse, Valerie Pestinger, Nicole Pfarr, Albrecht Stenzinger, Michel M van den Heuvel, Katrien Grünberg, Marjolijn J L Ligtenberg,

Stratification of patients for targeted and immune-based therapies requires extensive genomic profiling that enables sensitive detection of clinically relevant variants and interrogation of biomarkers, such as tumor mutational burden (TMB) and microsatellite instability (MSI). Detection of single and multiple nucleotide variants, copy number variants, MSI, and TMB was evaluated using ... Read more >>

J Mol Diagn (The Journal of molecular diagnostics : JMD)
[2020, 22(6):757-769]

Cited: 5 times

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Quantifying potential confounders of panel-based tumor mutational burden (TMB) measurement.

Jan Budczies, Daniel Kazdal, Michael Allgäuer, Petros Christopoulos, Eugen Rempel, Nicole Pfarr, Wilko Weichert, Stefan Fröhling, Michael Thomas, Solange Peters, Volker Endris, Peter Schirmacher, Albrecht Stenzinger,

<h4>Objectives</h4>Retrospective data including subgroup analyses in clinical studies have sparked strong interest in developing tumor mutational burden (TMB) as a predictive biomarker for immune checkpoint blockade. While individual factors influencing panel sequencing based measurement of TMB (psTMB) have been discussed in the recent literature, an integrative study quantifying, comparing and ... Read more >>

Lung Cancer (Lung cancer (Amsterdam, Netherlands))
[2020, 142:114-119]

Cited: 5 times

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Harmonization and Standardization of Panel-Based Tumor Mutational Burden Measurement: Real-World Results and Recommendations of the Quality in Pathology Study.

Albrecht Stenzinger, Volker Endris, Jan Budczies, Sabine Merkelbach-Bruse, Daniel Kazdal, Wolfgang Dietmaier, Nicole Pfarr, Udo Siebolts, Michael Hummel, Sylvia Herold, Johanna Andreas, Martin Zoche, Lars Tögel, Eugen Rempel, Jörg Maas, Diana Merino, Mark Stewart, Karim Zaoui, Matthias Schlesner, Hanno Glimm, Stefan Fröhling, Jeff Allen, David Horst, Gustavo Baretton, Claudia Wickenhauser, Markus Tiemann, Matthias Evert, Holger Moch, Thomas Kirchner, Reinhard Büttner, Peter Schirmacher, Andreas Jung, Florian Haller, Wilko Weichert, Manfred Dietel,

<h4>Introduction</h4>Tumor mutational burden (TMB) is a quantitative assessment of the number of somatic mutations within a tumor genome. Immunotherapy benefit has been associated with TMB assessed by whole-exome sequencing (wesTMB) and gene panel sequencing (psTMB). The initiatives of Quality in Pathology (QuIP) and Friends of Cancer Research have jointly addressed ... Read more >>

J Thorac Oncol (Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer)
[2020, 15(7):1177-1189]

Cited: 19 times

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Testing NTRK testing: Wet-lab and in silico comparison of RNA-based targeted sequencing assays.

Nicole Pfarr, Martina Kirchner, Ulrich Lehmann, Jonas Leichsenring, Sabine Merkelbach-Bruse, Julia Glade, Michael Hummel, Fabian Stögbauer, Annika Lehmann, Marcel Trautmann, Jörg Kumbrink, Andreas Jung, Wolfgang Dietmaier, Volker Endris, Daniel Kazdal, Matthias Evert, David Horst, Hans Kreipe, Thomas Kirchner, Eva Wardelmann, Ulrik Lassen, Reinhard Büttner, Wilko Weichert, Manfred Dietel, Peter Schirmacher, Albrecht Stenzinger,

NTRK fusions involving three neurotrophic tyrosine receptor kinase genes NTRK1, NTRK2, and NTRK3 and a variety of fusion partners were identified as oncogenic drivers across many cancer types. Drugs that target the chimeric protein product require the identification of the underlying gene fusion. This advocates the diagnostic use of molecular ... Read more >>

Genes Chromosomes Cancer (Genes, chromosomes & cancer)
[2020, 59(3):178-188]

Cited: 6 times

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Molecular characterization of hepatic epithelioid hemangioendothelioma reveals alterations in various genes involved in DNA repair, epigenetic regulation, signaling pathways, and cell cycle control.

Carolin Mogler, Ronald Koschny, Christoph E Heilig, Stefan Frohling, Peter Schirmacher, Wilko Weichert, Nicole Pfarr,

Epithelioid hemangioendotheliomas (EHE) of the liver are rare, low-malignant vascular tumors whose molecular pathogenesis is incompletely understood. The diagnosis of EHE is challenging, and the course of the disease can be highly variable. Therapeutic options for EHE are limited, including resection of primary and metastatic tumors, organ transplantation and rather ... Read more >>

Genes Chromosomes Cancer (Genes, chromosomes & cancer)
[2020, 59(2):106-110]

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Variant classification in precision oncology.

Jonas Leichsenring, Peter Horak, Simon Kreutzfeldt, Christoph Heining, Petros Christopoulos, Anna-Lena Volckmar, Olaf Neumann, Martina Kirchner, Carolin Ploeger, Jan Budczies, Christoph E Heilig, Barbara Hutter, Martina Fröhlich, Sebastian Uhrig, Daniel Kazdal, Michael Allgäuer, Alexander Harms, Eugen Rempel, Ulrich Lehmann, Michael Thomas, Nicole Pfarr, Ninel Azoitei, Irina Bonzheim, Ralf Marienfeld, Peter Möller, Martin Werner, Falko Fend, Melanie Boerries, Nikolas von Bubnoff, Silke Lassmann, Thomas Longerich, Michael Bitzer, Thomas Seufferlein, Nisar Malek, Wilko Weichert, Peter Schirmacher, Roland Penzel, Volker Endris, Benedikt Brors, Frederick Klauschen, Hanno Glimm, Stefan Fröhling, Albrecht Stenzinger,

Next-generation sequencing has become a cornerstone of therapy guidance in cancer precision medicine and an indispensable research tool in translational oncology. Its rapidly increasing use during the last decade has expanded the options for targeted tumor therapies, and molecular tumor boards have grown accordingly. However, with increasing detection of genetic ... Read more >>

Int J Cancer (International journal of cancer)
[2019, 145(11):2996-3010]

Cited: 6 times

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Several genotypes, one phenotype: PIK3CA/AKT1 mutation-negative hidradenoma papilliferum show genetic lesions in other components of the signalling network.

Nicole Pfarr, Michael Allgäuer, Katja Steiger, Wilko Weichert, Peter Schirmacher, Aurelia Noske, Albrecht Stenzinger,

About 60-70% of hidradenoma papilliferum (HP), a benign tumour of the anogenital region, were recently described to harbour mutations in major driver genes of the PI3K/AKT/MAPK-signalling pathways. However, the underlying genetic defects of the non-mutant cases are still unknown. Using a 409 gene panel, we employed targeted next generation sequencing ... Read more >>

Pathology (Pathology)
[2019, 51(4):362-368]

Cited: 0 times

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Mutational Diversity and Therapy Response in Breast Cancer: A Sequencing Analysis in the Neoadjuvant GeparSepto Trial.

Sibylle Loibl, Denise Treue, Jan Budczies, Karsten Weber, Albrecht Stenzinger, Wolfgang D Schmitt, Wilko Weichert, Paul Jank, Jenny Furlanetto, Frederick Klauschen, Thomas Karn, Nicole Pfarr, Gunter von Minckwitz, Markus Möbs, Christian Jackisch, Christine Sers, Andreas Schneeweiss, Peter A Fasching, Christian Schem, Michael Hummel, Marion van Mackelenbergh, Valentina Nekljudova, Michael Untch, Carsten Denkert,

<h4>Purpose</h4>Next-generation sequencing (NGS) can be used for comprehensive investigation of molecular events in breast cancer. We evaluated the relevance of genomic alterations for response to neoadjuvant chemotherapy (NACT) in the GeparSepto trial.<h4>Experimental design</h4>Eight hundred fifty-one pretherapeutic formalin-fixed paraffin-embedded (FFPE) core biopsies from GeparSepto study were sequenced. The panel included 16 ... Read more >>

Clin Cancer Res (Clinical cancer research : an official journal of the American Association for Cancer Research)
[2019, 25(13):3986-3995]

Cited: 7 times

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Composition and Clinical Impact of the Immunologic Tumor Microenvironment in Oral Squamous Cell Carcinoma.

Melanie Boxberg, Lena Leising, Katja Steiger, Moritz Jesinghaus, Aezlat Alkhamas, Marion Mielke, Nicole Pfarr, Carolin Götz, Klaus Dietrich Wolff, Wilko Weichert, Andreas Kolk,

Immunotherapy shows promising results and revolutionizes treatment of oral squamous cell carcinoma (OSCC). The immunologic microenvironment might have prognostic/predictive implications. Morphologic immunologic parameters (inflammatory infiltrate, stromal content, and budding activity [BA] [potentially indicating epithelial-mesenchymal transition]) were evaluated in 66 human primary therapy-naive OSCCs. Intraepithelial/stromal tumor-infiltrating lymphocytes (TILs; CD3<sup>+</sup>/CD4<sup>+</sup>/CD8<sup>+</sup>/CD4<sup>+</sup>FOXP3<sup>+</sup>/IL-17A<sup>+</sup>) were quantified, ... Read more >>

J Immunol (Journal of immunology (Baltimore, Md. : 1950))
[2019, 202(1):278-291]

Cited: 11 times

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