Full Text Journal Articles by
Author Morag Livingstone


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Defining immune correlates during latent and active chlamydial infection in sheep.

Sean R Wattegedera, Morag Livingstone, Stephen Maley, Mara Rocchi, Susan Lee, Yvonne Pang, Nick M Wheelhouse, Kevin Aitchison, Javier Palarea-Albaladejo, David Buxton, David Longbottom, Gary Entrican,

Ovine enzootic abortion (OEA) caused by the obligate intracellular bacterial pathogen Chlamydia abortus (C. abortus), is an endemic disease in most sheep-rearing countries worldwide. Following infection, C. abortus establishes a complex host-pathogen interaction with a latent phase in non-pregnant sheep followed by an active disease phase in the placenta during ... Read more >>

Vet. Res. (Veterinary research)
[2020, 51(1):75]

Cited: 0 times

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Complete Genome Sequence of Chlamydia avium PV 4360/2, Isolated from a Feral Pigeon in Italy.

Anna Maria Floriano, Sara Rigamonti, Francesco Comandatore, Erika Scaltriti, David Longbottom, Morag Livingstone, Karine Laroucau, Alessandra Gaffuri, Stefano Pongolini, Simone Magnino, Nadia Vicari,

We report here the whole-genome sequence of a Chlamydia avium isolate recovered from a feral pigeon in 1999 in Italy. Only one complete genome of a C. avium strain has been published so far. Future comparative analyses could provide valuable insights on the genomic evolution of the pathogen. ... Read more >>

Microbiol Resour Announc (Microbiology resource announcements)
[2020, 9(16):]

Cited: 0 times

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Proteomic characterisation of the Chlamydia abortus outer membrane complex (COMC) using combined rapid monolithic column liquid chromatography and fast MS/MS scanning.

David Longbottom, Morag Livingstone, Kevin D Aitchison, Lisa Imrie, Erin Manson, Nicholas Wheelhouse, Neil F Inglis,

Data are presented on the identification and partial characterisation of proteins comprising the chlamydial outer membrane complex (COMC) fraction of Chlamydia abortus (C. abortus)-the aetiological agent of ovine enzootic abortion. Inoculation with the COMC fraction is known to be highly effective in protecting sheep against experimental challenge and its constituent ... Read more >>

PLoS ONE (PloS one)
[2019, 14(10):e0224070]

Cited: 0 times

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Genomic evidence that the live Chlamydia abortus vaccine strain 1B is not attenuated and has the potential to cause disease.

David Longbottom, Michelle Sait, Morag Livingstone, Karine Laroucau, Konrad Sachse, Simon R Harris, Nicholas R Thomson, Helena M B Seth-Smith,

The live, temperature-attenuated vaccine strain 1B of Chlamydia abortus, the aetiological agent of ovine enzootic abortion (OEA), has been implicated in cases of vaccine breakdown. The aim of this study was to understand the nature of this attenuation through sequencing of the vaccine parent strain (AB7) and the derived mutant ... Read more >>

Vaccine (Vaccine)
[2018, 36(25):3593-3598]

Cited: 1 time

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Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS.

Morag Livingstone, Nicholas Wheelhouse, Hannah Ensor, Mara Rocchi, Stephen Maley, Kevin Aitchison, Sean Wattegedera, Kim Wilson, Michelle Sait, Victoria Siarkou, Evangelia Vretou, Gary Entrican, Mark Dagleish, David Longbottom,

This study investigated the pathogenesis of two variant strains (LLG and POS) of Chlamydia abortus, in comparison to a typical wild-type strain (S26/3) which is known to be responsible for late term abortion in small ruminants. Challenge with the three strains at mid-gestation resulted in similar pregnancy outcomes, with abortion ... Read more >>

PLoS ONE (PloS one)
[2017, 12(5):e0177653]

Cited: 2 times

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Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila.

Nicholas Wheelhouse, Allen Flockhart, Kevin Aitchison, Morag Livingstone, Jeanie Finlayson, Virginie Flachon, Eric Sellal, Mark P Dagleish, David Longbottom,

Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in cattle. Despite an increasing number of observational studies linking W. chondrophila infection to cattle abortion, little direct experimental evidence exists. Given this paucity of direct ... Read more >>

Sci Rep (Scientific reports)
[2016, 6:37150]

Cited: 3 times

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Analysis of Humoral Immune Responses to Surface and Virulence-Associated Chlamydia abortus Proteins in Ovine and Human Abortions by Use of a Newly Developed Line Immunoassay.

Jürgen Benjamin Hagemann, Ulrike Simnacher, David Longbottom, Morag Livingstone, Julia Maile, Erwin Soutschek, Gernot Walder, Katharina Boden, Konrad Sachse, Andreas Essig,

The obligate intracellular bacterium Chlamydia abortus is the causative agent of enzootic abortion of ewes and poses a significant zoonotic risk for pregnant women. Using proteomic analysis and gene expression library screening in a previous project, we identified potential virulence factors and candidates for serodiagnosis, of which nine were scrutinized ... Read more >>

J. Clin. Microbiol. (Journal of clinical microbiology)
[2016, 54(7):1883-1890]

Cited: 6 times

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Abortion in flocks vaccinated against enzootic abortion.

Morag Livingstone, Kevin Aitchison, David Longbottom,

Vet. Rec. (The Veterinary record)
[2014, 174(24):613-614]

Cited: 2 times

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Genome sequencing and comparative analysis of three Chlamydia pecorum strains associated with different pathogenic outcomes.

Michelle Sait, Morag Livingstone, Ewan M Clark, Nick Wheelhouse, Lucy Spalding, Bryan Markey, Simone Magnino, Frederick A Lainson, Garry S A Myers, David Longbottom,

Chlamydia pecorum is the causative agent of a number of acute diseases, but most often causes persistent, subclinical infection in ruminants, swine and birds. In this study, the genome sequences of three C. pecorum strains isolated from the faeces of a sheep with inapparent enteric infection (strain W73), from the ... Read more >>

BMC Genomics (BMC genomics)
[2014, 15:23]

Cited: 18 times

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Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep.

David Longbottom, Morag Livingstone, Stephen Maley, Arjan van der Zon, Mara Rocchi, Kim Wilson, Nicholas Wheelhouse, Mark Dagleish, Kevin Aitchison, Sean Wattegedera, Mintu Nath, Gary Entrican, David Buxton,

Latency is a key feature of the animal pathogen Chlamydia abortus, where infection remains inapparent in the non-pregnant animal and only becomes evident during a subsequent pregnancy. Often the first sign that an animal is infected is abortion occurring late in gestation. Despite this, little is understood of the underlying ... Read more >>

PLoS ONE (PloS one)
[2013, 8(2):e57950]

Cited: 23 times

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Processing of Chlamydia abortus polymorphic membrane protein 18D during the chlamydial developmental cycle.

Nick M Wheelhouse, Michelle Sait, Kevin Aitchison, Morag Livingstone, Frank Wright, Kevin McLean, Neil F Inglis, David G E Smith, David Longbottom,

Chlamydia possess a unique family of autotransporter proteins known as the Polymorphic membrane proteins (Pmps). While the total number of pmp genes varies between Chlamydia species, all encode a single pmpD gene. In both Chlamydia trachomatis (C. trachomatis) and C. pneumoniae, the PmpD protein is proteolytically cleaved on the cell ... Read more >>

PLoS ONE (PloS one)
[2012, 7(11):e49190]

Cited: 11 times

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Seroprevalence of chlamydial infection in cattle in Ireland.

Kim Wilson, Donal Sammin, Silke Harmeyer, Mintu Nath, Morag Livingstone, David Longbottom,

Although few studies have investigated the prevalence of chlamydial infections in cattle, reported prevalence rates vary hugely. In order to assess the prevalence of this infection in cattle in Ireland, serum samples (100 herds, 20 samples/herd) collected for statutory screening for brucellosis were examined by soluble chlamydial antigen indirect ELISA. ... Read more >>

Vet. J. (Veterinary journal (London, England : 1997))
[2012, 193(2):583-585]

Cited: 7 times

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Genome sequence of the Chlamydophila abortus variant strain LLG.

Michelle Sait, Ewan M Clark, Nick Wheelhouse, Morag Livingstone, Lucy Spalding, Victoria I Siarkou, Evangelia Vretou, David G E Smith, F Alex Lainson, David Longbottom,

Chlamydophila abortus is a common cause of ruminant abortion. Here we report the genome sequence of strain LLG, which differs genotypically and phenotypically from the wild-type strain S26/3. Genome sequencing revealed differences between LLG and S26/3 to occur in pseudogene content, in transmembrane head/inc family proteins, and in biotin biosynthesis ... Read more >>

J. Bacteriol. (Journal of bacteriology)
[2011, 193(16):4276-4277]

Cited: 8 times

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Identification, sequencing and molecular analysis of Chp4, a novel chlamydiaphage of Chlamydophila abortus belonging to the family Microviridae.

Michelle Sait, Morag Livingstone, Rebecca Graham, Neil F Inglis, Nick Wheelhouse, David Longbottom,

Members of the family Microviridae have been identified in a number of chlamydial species infecting humans (phage CPAR39 in Chlamydophila pneumoniae), other mammals (φCPG1 in Chlamydophila caviae, Chp2 in Chlamydophila abortus and Chp3 in Chlamydophila pecorum) and birds (Chp1 in Chlamydophila psittaci). This study describes the identification and genome sequencing ... Read more >>

J. Gen. Virol. (The Journal of general virology)
[2011, 92(Pt 7):1733-1737]

Cited: 10 times

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Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes.

Michelle Sait, Ewan M Clark, Nicholas Wheelhouse, Lucy Spalding, Morag Livingstone, Konrad Sachse, Bryan K Markey, Simone Magnino, Victoria I Siarkou, Evangelia Vretou, María R Caro, Raja Yaga, F Alex Lainson, David G E Smith, Frank Wright, David Longbottom,

This study used PCR-RFLP to investigate the genetic variability of pmp-encoding genes from fifty-two Chlamydophila abortus (C. abortus) strains originating from abortion cases from various geographical regions and host species. Six primer pairs were used to PCR-amplify DNA fragments encoding eighteen pmps. PCR products were digested using four restriction endonucleases ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2011, 151(3-4):284-290]

Cited: 3 times

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Inflammatory cytokine responses in a pregnant mouse model of Chlamydophila abortus infection.

Karen Kerr, Nicholas Wheelhouse, Morag Livingstone, Ian E Anderson, Gary Entrican, Declan McKeever, David Longbottom,

Chlamydophila abortus (C. abortus) is the aetiological agent of ovine enzootic abortion (OEA). The highly elevated expression of the pro-inflammatory cytokine tumour necrosis factor-alpha (TNFalpha) and low-level expression of interferon-gamma (IFNgamma) that are detected in C. abortus-infected placentas have been implicated in the pathogenesis of OEA. Late-term abortions similar to ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2010, 144(3-4):392-398]

Cited: 2 times

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Transcriptional analysis of in vitro expression patterns of Chlamydophila abortus polymorphic outer membrane proteins during the chlamydial developmental cycle.

Nicholas Wheelhouse, Kevin Aitchison, Lucy Spalding, Morag Livingstone, David Longbottom,

Chlamydophila abortus is the aetiological agent of ovine enzootic abortion. Sequencing, annotation and comparative analysis of the genome of C. abortus strain S26/3 has revealed variation in the loci encoding the polymorphic membrane proteins (Pmps). These Pmps resemble autotransporter proteins of the type V secretion system, suggesting an important role ... Read more >>

Vet. Res. (Veterinary research)
[2009, 40(5):47]

Cited: 5 times

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Comparative evaluation of eight serological assays for diagnosing Chlamydophila abortus infection in sheep.

Kim Wilson, Morag Livingstone, David Longbottom,

Chlamydophila abortus is one of the principal causes of late-term abortion (enzootic abortion of ewes or EAE) in sheep across Europe. Serological diagnosis of EAE is routinely carried out by the complement fixation test, although the interpretation of results can often be difficult because of cross reaction with Chlamydophila pecorum, ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2009, 135(1-2):38-45]

Cited: 13 times

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Molecular detection of Chlamydophila abortus in post-abortion sheep at oestrus and subsequent lambing.

Morag Livingstone, Nicholas Wheelhouse, Stephen W Maley, David Longbottom,

Enzootic abortion of ewes (EAE), resulting from infection with the bacterium Chlamydophila abortus (C. abortus), is a major cause of lamb loss in Europe. The purpose of this study was to assess the potential impact of the shedding of organisms in post-abortion ewes at oestrus and subsequent lambing on the ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2009, 135(1-2):134-141]

Cited: 14 times

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Recent developments in the laboratory diagnosis of chlamydial infections.

Konrad Sachse, Evangelia Vretou, Morag Livingstone, Nicole Borel, Andreas Pospischil, David Longbottom,

There are two main approaches to diagnosing infections by Chlamydia and Chlamydophila spp. in mammals and birds. The first involves the direct detection of the agent in tissue or swab samples, while the second involves the serological screening of blood samples for the presence of anti-chlamydial antibodies. Ultimately, the test ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2009, 135(1-2):2-21]

Cited: 45 times

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The Chlamydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies variation.

Nicholas R Thomson, Corin Yeats, Kenneth Bell, Matthew T G Holden, Stephen D Bentley, Morag Livingstone, Ana M Cerdeño-Tárraga, Barbara Harris, Jon Doggett, Doug Ormond, Karen Mungall, Kay Clarke, Theresa Feltwell, Zahra Hance, Mandy Sanders, Michael A Quail, Claire Price, Bart G Barrell, Julian Parkhill, David Longbottom,

The obligate intracellular bacterial pathogen Chlamydophila abortus strain S26/3 (formerly the abortion subtype of Chlamydia psittaci) is an important cause of late gestation abortions in ruminants and pigs. Furthermore, although relatively rare, zoonotic infection can result in acute illness and miscarriage in pregnant women. The complete genome sequence was determined ... Read more >>

Genome Res. (Genome research)
[2005, 15(5):629-640]

Cited: 117 times

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Antibody responses to recombinant protein fragments of the major outer membrane protein and polymorphic outer membrane protein POMP90 in Chlamydophila abortus-infected pregnant sheep.

Morag Livingstone, Gary Entrican, Sean Wattegedera, David Buxton, Iain J McKendrick, David Longbottom,

Chlamydophila abortus is one of the major causes of infectious abortion in pregnant sheep (enzootic abortion of ewes or EAE) worldwide. Organisms shed in infected placentas and uterine discharges at lambing time are the main sources of environmental contamination, responsible for transmission to susceptible animals and possible human contacts. In ... Read more >>

Clin. Diagn. Lab. Immunol. (Clinical and diagnostic laboratory immunology)
[2005, 12(6):770-777]

Cited: 20 times

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Serological diagnosis of ovine enzootic abortion by enzyme-linked immunosorbent assay with a recombinant protein fragment of the polymorphic outer membrane protein POMP90 of Chlamydophila abortus.

David Longbottom, Susan Fairley, Stephanie Chapman, Evgenia Psarrou, Evangelia Vretou, Morag Livingstone,

Ovine enzootic abortion (OEA) resulting from infection of sheep and goats with Chlamydophila abortus is of major economic importance worldwide. Over the last 50 years the serological diagnosis of infection has been based mainly on the complement fixation test (CFT), which lacks both sensitivity and specificity because of cross-reactive antibodies ... Read more >>

J. Clin. Microbiol. (Journal of clinical microbiology)
[2002, 40(11):4235-4243]

Cited: 23 times

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