Full Text Journal Articles by
Author Michael Przybylski

Advertisement

Find full text journal articles






Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination.

Loredana-Mirela Lupu, Pascal Wiegand, Daria Holdschick, Delia Mihoc, Stefan Maeser, Stephan Rawer, Friedemann Völklein, Ebrahim Malek, Frederik Barka, Sascha Knauer, Christina Uth, Julia Hennermann, Wolfgang Kleinekofort, Andreas Hahn, Günes Barka, Michael Przybylski,

Analytical methods for molecular characterization of diagnostic or therapeutic targets have recently gained high interest. This review summarizes the combination of mass spectrometry and surface plasmon resonance (SPR) biosensor analysis for identification and affinity determination of protein interactions with antibodies and DNA-aptamers. The binding constant (K<sub>D</sub>) of a protein-antibody complex ... Read more >>

Int J Mol Sci (International journal of molecular sciences)
[2021, 22(23):]

Cited: 0 times

View full text PDF listing >>



Enzymatic diagnosis of neuronal lipofuscinoses in dried blood spots using substrates for concomitant tandem mass spectrometry and fluorimetry.

Stefan Maeser, Brindusa-Alina Petre, Laura Ion, Stephan Rawer, Alfried Kohlschütter, Filippo M Santorelli, Alessandro Simonati, Angela Schulz, Michael Przybylski,

Neuronal ceroid lipofuscinoses (NCLs) are a group of neurodegenerative diseases predominantly in childhood that are characterized by psychomotor deterioration, epilepsy, and early death of patients. The NCLs analyzed in the present study are caused by defects of the specific enzymes, CLN1 (palmitoyl protein thioesterase 1; PPT1), CLN2 (tripeptidyl peptidase 1; ... Read more >>

J Mass Spectrom (Journal of mass spectrometry : JMS)
[2021, 56(1):e4675]

Cited: 0 times

View full text PDF listing >>



Advertisement

Antibody Epitope and Affinity Determination of the Myocardial Infarction Marker Myoglobin by SPR-Biosensor Mass Spectrometry.

Delia Mihoc, Loredana-Mirela Lupu, Pascal Wiegand, Wolfgang Kleinekofort, Oliver Müller, Friedemann Völklein, Michael O Glocker, Frederik Barka, Günes Barka, Michael Przybylski,

Myoglobin (MG) is a biomarker for heart muscle injury, making it a potential target protein for early detection of myocardial infarction. Elevated myoglobin levels alone have low specificity for acute myocardial infarction (AMI) but in combination with cardiac troponin T have been considered highly efficient diagnostic biomarkers. Myoglobin is a ... Read more >>

J Am Soc Mass Spectrom (Journal of the American Society for Mass Spectrometry)
[2021, 32(1):106-113]

Cited: 0 times

View full text PDF listing >>



Unveiling the molecular mechanisms underpinning biorecognition of early-glycated human serum albumin and receptor for advanced glycation end products.

Anna Tramarin, Marina Naldi, Genny Degani, Loredana Lupu, Pascal Wiegand, Angelica Mazzolari, Alessandra Altomare, Giancarlo Aldini, Laura Popolo, Giulio Vistoli, Michael Przybylski, Manuela Bartolini,

Serum levels of early-glycated albumin are significantly increased in patients with diabetes mellitus and may play a role in worsening inflammatory status and sustaining diabetes-related complications. To investigate possible pathological recognition involving early-glycated albumin and the receptor for advanced glycation end products (RAGE), an early-glycated human serum albumin (HSAgly), with ... Read more >>

Anal Bioanal Chem (Analytical and bioanalytical chemistry)
[2020, 412(18):4245-4259]

Cited: 0 times

View full text PDF listing >>



Molecular Epitope Determination of Aptamer Complexes of the Multidomain Protein C-Met by Proteolytic Affinity-Mass Spectrometry.

Loredana Lupu, Pascal Wiegand, Nico Hüttmann, Stephan Rawer, Wolfgang Kleinekofort, Irina Shugureva, Anna S Kichkailo, Felix N Tomilin, Alexander Lazarev, Maxim V Berezovski, Michael Przybylski,

C-Met protein is a glycosylated receptor tyrosine kinase of the hepatocyte growth factor (HGF), composed of an α and a β chain. Upon ligand binding, C-Met transmits intracellular signals by a unique multi-substrate docking site. C-Met can be aberrantly activated leading to tumorigenesis and other diseases, and has been recognized ... Read more >>

ChemMedChem (ChemMedChem)
[2020, 15(4):363-369]

Cited: 1 time

View full text PDF listing >>



Epitope Identification and Affinity Determination of an Inhibiting Human Antibody to Interleukin IL8 (CXCL8) by SPR- Biosensor-Mass Spectrometry Combination.

Pascal Wiegand, Loredana Lupu, Nico Hüttmann, Julia Wack, Stephan Rawer, Michael Przybylski, Katja Schmitz,

The polypeptide chemokine Interleukin-8 (IL8) plays a crucial role in inflammatory processes in humans. IL8 is involved in chronic inflammatory lung diseases, rheumatoid arthritis, and cancer. Previous studies have shown that the interaction of IL8 with its natural receptors CXCR1 and CXCR2 is critical in these diseases. Antibodies have been ... Read more >>

J Am Soc Mass Spectrom (Journal of the American Society for Mass Spectrometry)
[2020, 31(1):109-116]

Cited: 2 times

View full text PDF listing >>



Epitope and affinity determination of recombinant Mycobacterium tuberculosis Ag85B antigen towards anti-Ag85 antibodies using proteolytic affinity-mass spectrometry and biosensor analysis.

Francesca Rinaldi, Loredana Lupu, Hendrik Rusche, Zdeněk Kukačka, Sara Tengattini, Roberta Bernardini, Luciano Piubelli, Teodora Bavaro, Stefan Maeser, Loredano Pollegioni, Enrica Calleri, Michael Przybylski, Caterina Temporini,

Tuberculosis (TB) is the first cause of death from infectious diseases worldwide. Only a single anti-TB vaccine is currently available for clinical use, but its efficacy is not achieved with certainty. The aim of this work is to provide a basis for the rational design of a neo-glycoconjugate vaccine against ... Read more >>

Anal Bioanal Chem (Analytical and bioanalytical chemistry)
[2019, 411(2):439-448]

Cited: 2 times

View full text PDF listing >>



Epitope Ligand Binding Sites of Blood Group Oligosaccharides in Lectins Revealed by Pressure-Assisted Proteolytic Excision Affinity Mass Spectrometry.

Yannick Baschung, Loredana Lupu, Adrian Moise, Michael Glocker, Stephan Rawer, Alexander Lazarev, Michael Przybylski,

Affinity mass spectrometry using selective proteolytic excision and extraction combined with MALDI and ESI mass spectrometry has been applied to the identification of epitope binding sites of lactose, GalNac, and blood group oligosaccharides in two blood group-specific lectins, human galectin-3 and glycine max lectin. The epitope peptides identified comprise all ... Read more >>

J Am Soc Mass Spectrom (Journal of the American Society for Mass Spectrometry)
[2018, 29(9):1881-1891]

Cited: 1 time

View full text PDF listing >>



Antibody Epitope of Human α-Galactosidase A Revealed by Affinity Mass Spectrometry: A Basis for Reversing Immunoreactivity in Enzyme Replacement Therapy of Fabry Disease.

Zdenek Kukacka, Marius Iurascu, Loredana Lupu, Hendrik Rusche, Mary Murphy, Lorenzo Altamore, Fabio Borri, Stefan Maeser, Anna Maria Papini, Julia Hennermann, Michael Przybylski,

α-Galactosidase (αGal) is a lysosomal enzyme that hydrolyses the terminal α-galactosyl moiety from glycosphingolipids. Mutations in the encoding genes for αGal lead to defective or misfolded enzyme, which results in substrate accumulation and subsequent organ dysfunction. The metabolic disease caused by a deficiency of human α-galactosidase A is known as Fabry ... Read more >>

ChemMedChem (ChemMedChem)
[2018, 13(9):909-915]

Cited: 1 time

View full text PDF listing >>



Molecular characterization of the β-amyloid(4-10) epitope of plaque specific Aβ antibodies by affinity-mass spectrometry using alanine site mutation.

Raluca Ștefănescu, Loredana Lupu, Marilena Manea, Roxana E Iacob, Michael Przybylski,

Alzheimer disease is a neurodegenerative disease affecting an increasing number of patients worldwide. Current therapeutic strategies are directed to molecules capable to block the aggregation of the β-amyloid(1-42) (Aβ) peptide and its shorter naturally occurring peptide fragments into toxic oligomers and amyloid fibrils. Aβ-specific antibodies have been recently developed as ... Read more >>

J Pept Sci (Journal of peptide science : an official publication of the European Peptide Society)
[2018, 24(1):]

Cited: 5 times

View full text PDF listing >>



Erratum: Growth of organic crystals via attachment and transformation of nanoscopic precursors.

Yuan Jiang, Matthias Kellermeier, Denis Gebauer, Zihao Lu, Rose Rosenberg, Adrian Moise, Michael Przybylski, Helmut Cölfen,

This corrects the article DOI: 10.1038/ncomms15933. ... Read more >>

Nat Commun (Nature communications)
[2017, 8:16132]

Cited: 0 times

View full text PDF listing >>



An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry.

Marius-Ionuţ Iuraşcu, Osiris Marroquin Belaunzanar, Claudia Cozma, Ulf Petrausch, Christoph Renner, Michael Przybylski,

HLA-B27 homodimer formation is believed to be a hallmark of HLA-B27 associated spondyloarthritides. Recently, we have generated a homodimer-specific monoclonal antibody (HD6) and have demonstrated that HLA-B27 homodimer complexes are present on monocytes of healthy HLA-B27 gene carriers at low levels, with significantly increased levels at active disease. The capability ... Read more >>

J Am Soc Mass Spectrom (Journal of the American Society for Mass Spectrometry)
[2016, 27(6):1105-1112]

Cited: 3 times

View full text PDF listing >>



Substrate and Substrate-Mimetic Chaperone Binding Sites in Human α-Galactosidase A Revealed by Affinity-Mass Spectrometry.

Adrian Moise, Stefan Maeser, Stephan Rawer, Frederike Eggers, Mary Murphy, Jeff Bornheim, Michael Przybylski,

Fabry disease (FD) is a rare metabolic disorder of a group of lysosomal storage diseases, caused by deficiency or reduced activity of the enzyme α-galactosidase. Human α-galactosidase A (hαGAL) hydrolyses the terminal α-galactosyl moiety from glycosphingolipids, predominantly globotriaosylceramide (Gb3). Enzyme deficiency leads to incomplete or blocked breakdown and progressive accumulation ... Read more >>

J Am Soc Mass Spectrom (Journal of the American Society for Mass Spectrometry)
[2016, 27(6):1071-1078]

Cited: 2 times

View full text PDF listing >>



Early infantile neuronal ceroid lipofuscinosis (CLN10 disease) associated with a novel mutation in CTSD.

Stefano Doccini, Stefano Sartori, Stefan Maeser, Francesco Pezzini, Sara Rossato, Francesca Moro, Irene Toldo, Michael Przybylski, Filippo M Santorelli, Alessandro Simonati,

J Neurol (Journal of neurology)
[2016, 263(5):1029-1032]

Cited: 10 times

View full text PDF listing >>



Identification and affinity-quantification of ß-amyloid and α-synuclein polypeptides using on-line SAW-biosensor-mass spectrometry.

Stefan Slamnoiu, Camelia Vlad, Mihaela Stumbaum, Adrian Moise, Kathrin Lindner, Nicole Engel, Mar Vilanova, Mireia Diaz, Christiaan Karreman, Marcel Leist, Thomas Ciossek, Bastian Hengerer, Marta Vilaseca, Michael Przybylski,

Bioaffinity analysis using a variety of biosensors has become an established tool for detection and quantification of biomolecular interactions. Biosensors, however, are generally limited by the lack of chemical structure information of affinity-bound ligands. On-line bioaffinity-mass spectrometry using a surface-acoustic wave biosensor (SAW-MS) is a new combination providing the simultaneous ... Read more >>

J Am Soc Mass Spectrom (Journal of the American Society for Mass Spectrometry)
[2014, 25(8):1472-1481]

Cited: 5 times

View full text PDF listing >>



Increased levels of antigen-bound β-amyloid autoantibodies in serum and cerebrospinal fluid of Alzheimer's disease patients.

Madalina Maftei, Franka Thurm, Cathrin Schnack, Hayrettin Tumani, Markus Otto, Thomas Elbert, Iris-Tatjana Kolassa, Michael Przybylski, Marilena Manea, Christine A F von Arnim,

Recent studies have suggested a protective role of physiological β-amyloid autoantibodies (Aβ-autoantibodies) in Alzheimer's disease (AD). However, the determination of both free and dissociated Aβ-autoantibodies in serum hitherto has yielded inconsistent results regarding their function and possible biomarker value. Here we report the application of a new sandwich enzyme-linked immunosorbent ... Read more >>

PLoS One (PloS one)
[2013, 8(7):e68996]

Cited: 22 times

View full text PDF listing >>



Testing the feasibility of fully automated chip-based nanoelectrospray ionization mass spectrometry as a novel tool for rapid diagnosis of Fabry disease.

Corina Flangea, Cristina Mosoarca, Claudia Cozma, Mirela Galusca, Michael Przybylski, Alina D Zamfir,

Fabry condition, a lysosomal storage disease (LSD) is characterized by the absence or reduction of the α-galactosidase A activity. Recently, a new diagnostic method for detection of α-galactosidase activity from dried blood spots (DBS) using a chemical substrate and quantification of reaction mixture was developed. To improve this method in ... Read more >>

Electrophoresis (Electrophoresis)
[2013, 34(11):1572-1580]

Cited: 5 times

View full text PDF listing >>



When is mass spectrometry combined with affinity approaches essential? A case study of tyrosine nitration in proteins.

Brînduşa-Alina Petre, Martina Ulrich, Mihaela Stumbaum, Bogdan Bernevic, Adrian Moise, Gerd Döring, Michael Przybylski,

Tyrosine nitration in proteins occurs under physiologic conditions and is increased at disease conditions associated with oxidative stress, such as inflammation and Alzheimer's disease. Identification and quantification of tyrosine-nitrations are crucial for understanding nitration mechanism(s) and their functional consequences. Mass spectrometry (MS) is best suited to identify nitration sites, but ... Read more >>

J Am Soc Mass Spectrom (Journal of the American Society for Mass Spectrometry)
[2012, 23(11):1831-1840]

Cited: 9 times

View full text PDF listing >>



Why the structure but not the activity of the immunoproteasome subunit low molecular mass polypeptide 2 rescues antigen presentation.

Michael Basler, Christoph Lauer, Jacqueline Moebius, Reinhold Weber, Michael Przybylski, Alexei F Kisselev, Christopher Tsu, Marcus Groettrup,

The proteasome is responsible for the generation of most epitopes presented on MHC class I molecules. Treatment of cells with IFN-γ leads to the replacement of the constitutive catalytic subunits β1, β2, and β5 by the inducible subunits low molecular mass polypeptide (LMP) 2 (β1i), multicatalytic endopeptidase complex-like-1 (β2i), and ... Read more >>

J Immunol (Journal of immunology (Baltimore, Md. : 1950))
[2012, 189(4):1868-1877]

Cited: 21 times

View full text PDF listing >>



Epitope structure and binding affinity of single chain llama anti-β-amyloid antibodies revealed by proteolytic excision affinity-mass spectrometry.

Gabriela Paraschiv, Cécile Vincke, Paulina Czaplewska, Marilena Manea, Serge Muyldermans, Michael Przybylski,

ß-Amyloid (Aß) immunotherapy has become a promising strategy for reducing the level of Aß in brain. New immunological approaches have been recently proposed for rapid, early diagnosis, and molecular treatment of neurodegenerative diseases related to Alzheimer's Disease (AD). The combination of proteolytic epitope excision and extraction and mass spectrometry using ... Read more >>

J Mol Recognit (Journal of molecular recognition : JMR)
[2013, 26(1):1-9]

Cited: 10 times

View full text PDF listing >>



Application of MALDI-TOF-mass spectrometry to proteome analysis using stain-free gel electrophoresis.

Iuliana Susnea, Bogdan Bernevic, Michael Wicke, Li Ma, Shuying Liu, Karl Schellander, Michael Przybylski,

The combination of MALDI-TOF-mass spectrometry with gel electrophoretic separation using protein visualization by staining procedures involving such as Coomassie Brilliant Blue has been established as a widely used approach in proteomics. Although this approach has been shown to present high detection sensitivity, drawbacks and limitations frequently arise from the significant ... Read more >>

Top Curr Chem (Topics in current chemistry)
[2013, 331:37-54]

Cited: 5 times

View full text PDF listing >>



Antigen-bound and free β-amyloid autoantibodies in serum of healthy adults.

Madalina Maftei, Franka Thurm, Vera Maria Leirer, Christine A F von Arnim, Thomas Elbert, Michael Przybylski, Iris-Tatjana Kolassa, Marilena Manea,

Physiological β-amyloid autoantibodies (Aβ-autoantibodies) are currently investigated as potential diagnostic and therapeutic tools for Alzheimer's disease (AD). In previous studies, their determination in serum and cerebrospinal fluid (CSF) using indirect ELISA has provided controversial results, which may be due to the presence of preformed Aβ antigen-antibody immune complexes. Based on ... Read more >>

PLoS One (PloS one)
[2012, 7(9):e44516]

Cited: 6 times

View full text PDF listing >>



Interaction structure of the complex between neuroprotective factor humanin and Alzheimer's β-amyloid peptide revealed by affinity mass spectrometry and molecular modeling.

Madalina Maftei, Xiaodan Tian, Marilena Manea, Thomas E Exner, Daniel Schwanzar, Christine A F von Arnim, Michael Przybylski,

Humanin (HN) is a linear 24-aa peptide recently detected in human Alzheimer's disease (AD) brain. HN specifically inhibits neuronal cell death in vitro induced by ß-amyloid (Aß) peptides and by amyloid precursor protein and its gene mutations in familial AD, thereby representing a potential therapeutic lead structure for AD; however, ... Read more >>

J Pept Sci (Journal of peptide science : an official publication of the European Peptide Society)
[2012, 18(6):373-382]

Cited: 20 times

View full text PDF listing >>



Correction: Antigen-Bound and Free β-Amyloid Autoantibodies in Serum of Healthy Adults

Madalina Maftei, Franka Thurm, Vera Maria Leirer, Christine A. F. von Arnim, Thomas Elbert, Michael Przybylski, Iris-Tatjana Kolassa, Marilena Manea,

PLoS One (PloS one)
[2012, 7(11):]

Cited: 0 times

View full text PDF listing >>



Characterization of oligomerization-aggregation products of neurodegenerative target proteins by ion mobility mass spectrometry.

Camelia Vlad, Marius Ionut Iurascu, Stefan Slamnoiu, Bastian Hengerer, Michael Przybylski,

Protein amyloidogenesis is generally considered to be a major cause of two most severe neurodegenerative disorders, Parkinson's disease (PD) and Alzheimer's disease (AD). Formation and accumulation of fibrillar aggregates and plaques derived from α-synuclein (α-Syn) and ß-amyloid (Aß) polypeptide in brain have been recognized as characteristics of Parkinson's disease and ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2012, 896:399-412]

Cited: 6 times

View full text PDF listing >>



Advertisement


Disclaimer

1.0552 s