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Acceptability of dual HIV/syphilis rapid test in community- and home-based testing strategy among transgender women in Buenos Aires, Argentina.

Virginia Zalazar, Claudia E Frola, Ana Gun, Pablo D Radusky, Natalia K Panis, Nadir F Cardozo, Solange Fabian, Mariana I Duarte, Inés Aristegui, Pedro Cahn, Omar Sued,

<h4>Background</h4>Little is known of acceptability and feasibility of dual HIV and syphilis rapid tests in community- and home-based provider-initiated strategies among transgender women (TGW), in Latin America. Objectives were (1) to assess the acceptability of this strategy and, (2) to determine the percentage of positive results of HIV and syphilis, ... Read more >>

Int J STD AIDS (International journal of STD & AIDS)
[2021, :956462420979852]

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Two Self-Incompatibility Sites Occur Simultaneously in the Same Acianthera Species (Orchidaceae, Pleurothallidinae).

Mariana Oliveira Duarte, Denise Maria Trombert Oliveira, Eduardo Leite Borba,

In most species of Pleurothallidinae, the self-incompatibility site occurs in the stylar canal inside the column, which is typical of gametophytic self-incompatibility (GSI). However, in some species of <i>Acianthera</i>, incompatible pollen tubes with anomalous morphology reach the ovary, as those are obstructed in the column. We investigated if a distinct ... Read more >>

Plants (Basel) (Plants (Basel, Switzerland))
[2020, 9(12):]

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Pulse Consumption among Portuguese Adults: Potential Drivers and Barriers towards a Sustainable Diet.

Mariana Duarte, Marta Vasconcelos, Elisabete Pinto,

The transition from diets rich in animal products to plant-based protein foods-like pulses-is crucial, for both environmental sustainability and human health. The aim of this study was to characterize the current consumption and to describe the drivers of and barriers to pulse intake in Portugal. Using a quantitative approach, a ... Read more >>

Nutrients (Nutrients)
[2020, 12(11):]

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Corrigendum to "Leishmania infantum pyridoxal kinase evaluated in a recombinant protein and DNA vaccine to protects against visceral leishmaniasis". Molecular Immunology 124 (2020) 161-171.

João A Oliveira-da-Silva, Daniela P Lage, Fernanda F Ramos, Amanda S Machado, Grasiele S V Tavares, Débora V C Mendonça, Isabela A G Pereira, Vívian T Martins, Lívia M Carvalho, Fernanda Ludolf, Thaís T O Santos, Thiago A R Reis, Camila S Freitas, Raquel S Bandeira, Alessandra M Silva, Lourena E Costa, Jamil S Oliveira, Mariana C Duarte, Bruno M Roatt, Antônio L Teixeira, Eduardo A F Coelho,

Mol Immunol (Molecular immunology)
[2020, 128:55]

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Parasitological and immunological evaluation of a novel chemotherapeutic agent against visceral leishmaniasis.

Isabela A G Pereira, Débora V C Mendonça, Grasiele S V Tavares, Daniela P Lage, Fernanda F Ramos, João A Oliveira-da-Silva, Luciana M R Antinarelli, Amanda S Machado, Lívia M Carvalho, Ana Maria R S Carvalho, Iorrana V Salustiano, Thiago A R Reis, Raquel S Bandeira, Alessandra M Silva, Vívian T Martins, Miguel A Chávez-Fumagalli, Maria V Humbert, Bruno M Roatt, Mariana C Duarte, Daniel Menezes-Souza, Elaine S Coimbra, João Paulo V Leite, Eduardo A F Coelho, Denise U Gonçalves,

<h4>Aims</h4>Treatment for visceral leishmaniasis (VL) is hampered by the toxicity and/or high cost of drugs, as well as by emergence of parasite resistance. Therefore, there is an urgent need for new antileishmanial agents.<h4>Methods and results</h4>In this study, the antileishmanial activity of a diprenylated flavonoid called 5,7,3,4'-tetrahydroxy-6,8-diprenylisoflavone (CMt) was tested against ... Read more >>

Parasite Immunol (Parasite immunology)
[2020, 42(12):e12784]

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A Leishmania amastigote-specific hypothetical protein evaluated as recombinant protein plus Th1 adjuvant or DNA plasmid-based vaccine to protect against visceral leishmaniasis.

João A Oliveira-da-Silva, Amanda S Machado, Fernanda F Ramos, Grasiele S V Tavares, Daniela P Lage, Débora V C Mendonça, Isabela A G Pereira, Thaís T O Santos, Vívian T Martins, Lívia M Carvalho, Camila S Freitas, Fernanda Ludolf, Thiago A R Reis, Raquel S Bandeira, Alessandra M Silva, Lourena E Costa, Jamil S Oliveira, Mariana C Duarte, Bruno M Roatt, Antônio L Teixeira, Eduardo A F Coelho,

Most studies evaluating vaccine candidates against visceral leishmaniasis (VL) have used parasite promastigote-expressed antigens; however, Leishmania proteins expressed in the amastigote forms should be considered, since few hours after infection this stage comes into contact with the host immune system and is responsible for the development of the disease. In ... Read more >>

Cell Immunol (Cellular immunology)
[2020, 356:104194]

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A new Leishmania hypothetical protein can be used for accurate serodiagnosis of canine and human visceral leishmaniasis and as a potential prognostic marker for human disease.

Amanda S Machado, Fernanda F Ramos, Thaís T O Santos, Lourena E Costa, Fernanda Ludolf, Daniela P Lage, Raquel S Bandeira, Grasiele S V Tavares, João A Oliveira-da-Silva, Bethina T Steiner, Ana T Chaves, Jamil S Oliveira, Miguel A Chávez-Fumagalli, Danielle F de Magalhães-Soares, Julia A G Silveira, Mariana C Duarte, Ricardo A Machado-de-Ávila, Sandra Lyon, Denise U Gonçalves, Rachel B Caligiorne, Eduardo A F Coelho,

Distinct antigens have been evaluated with diagnostic purpose for canine and human visceral leishmaniasis (VL), and variable sensitivity and specificity values have been obtained in the assays. In the present study, a Leishmania infantum hypothetical protein called LiHyG, which was identified in an immunoproteomics study in Leishmania infantum amastigote extracts ... Read more >>

Exp Parasitol (Experimental parasitology)
[2020, 216:107941]

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Leishmania infantum pyridoxal kinase evaluated in a recombinant protein and DNA vaccine to protects against visceral leishmaniasis.

João A Oliveira-da-Silva, Daniela P Lage, Fernanda F Ramos, Amanda S Machado, Grasiele S V Tavares, Débora V C Mendonça, Isabela A G Pereira, Vívian T Martins, Lívia M Carvalho, Fernanda Ludolf, Thaís T O Santos, Thiago A R Reis, Camila S Oliveira, Raquel S Bandeira, Alessandra M Silva, Lourena E Costa, Jamil S Oliveira, Mariana C Duarte, Daniel Menezes-Souza, Bruno M Roatt, Antônio L Teixeira, Eduardo A F Coelho,

Leishmania infantum pyridoxal kinase (PK) protein was characterized after an immunoproteomics screening performed with the sera from patients suffering visceral leishmaniasis (VL). Since it was recognized by sera of mammalian hosts infected by a viscerotropic Leishmania species, PK could emerge as a new vaccine candidate against disease, due to its ... Read more >>

Mol Immunol (Molecular immunology)
[2020, 124:161-171]

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Misdosing of Non-Vitamin K Antagonist Oral Anticoagulants in Primary Care.

Carlos Seiça Cardoso, João André Sousa, Pedro Simões, Beatriz Silva, Ana Albuquerque, Ana Esperança, Ana Cibrão, Artur Correia, Joana Gonçalves, Joana Mortágua, José Eduardo Almeida, Leonor Oliveira, José Garcia, Mariana Duarte, Mariana Loureiro, Marta Costa E Silva, Marta Fraga, Paulo Lopes, Rui Brandão, Susana Miguel, Teresa Queirós, Gustavo Santo, Fernando Silva, João Sargento-Freitas,

<h4>Purpose</h4>Prescription patterns of non-vitamin K antagonist oral anticoagulants (NOACs) are unknown among primary care physicians, where most patients with nonvalvular atrial fibrillation (NVAF) are diagnosed and followed up. The goal of this study was to evaluate overdosing and underdosing of NOACs in patients with NVAF followed up in primary care ... Read more >>

Clin Ther (Clinical therapeutics)
[2020, 42(6):1132-1136.e1]

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Evaluation of the protective efficacy of a Leishmania protein associated with distinct adjuvants against visceral leishmaniasis and in vitro immunogenicity in human cells.

Patrícia A F Ribeiro, Daniel S Dias, Daniela P Lage, Débora V C Mendonça, Danniele L Vale, Fernanda F Ramos, Lívia M Carvalho, Ana Maria R S Carvalho, Bethina T Steiner, Marjorie C Roque, João A Oliveira-da-Silva, Jamil S Oliveira, Grasiele S V Tavares, Vívian T Martins, Miguel A Chávez-Fumagalli, Bruno M Roatt, Ricardo L F Moreira, Daniel Menezes-Souza, Mariana C Duarte, Mônica C Oliveira, Ricardo A Machado-de-Ávila, Antônio L Teixeira, Eduardo A F Coelho,

The treatment against visceral leishmaniasis (VL) presents problems, mainly related to the toxicity and/or high cost of the drugs. In this context, a prophylactic vaccination is urgently required. In the present study, a Leishmania protein called LiHyE, which was suggested recently as an antigenic marker for canine and human VL, ... Read more >>

Parasitol Res (Parasitology research)
[2020, 119(8):2609-2622]

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An immunoproteomics approach to identify Leishmania infantum proteins to be applied for the diagnosis of visceral leishmaniasis and human immunodeficiency virus co-infection.

Amanda S Machado, Fernanda F Ramos, João A Oliveira-da-Silva, Thaís T O Santos, Grasiele S V Tavares, Lourena E Costa, Daniela P Lage, André Teixeira-Ferreira, Jonas Perales, Ana Paula Fernandes, Ricardo L F Moreira, Mariana C Duarte, Unaí Tupinambás, Rachel B Caligiorne, Gláucia F Cota, Eduardo A F Coelho, Fernanda Ludolf,

The co-infection between visceral leishmaniasis (VL) and human immunodeficiency virus (HIV) has increased in several countries in the world. The current serological tests are not suitable since they present low sensitivity to detect the most of VL/HIV cases, and a more precise diagnosis should be performed. In this context, in ... Read more >>

Parasitology (Parasitology)
[2020, 147(9):932-939]

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Osteoarticular infections in paediatric sickle cell disease: in the era of multidrugresistant bacteria.

Catarina Gouveia, Mariana Duarte, Susana Norte, Pedro Alves, Paula Kjöllerström, Maria Brito, Delfin Tavares,

Br J Haematol (British journal of haematology)
[2020, 189(4):e147-e150]

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Evaluation of Leishmania infantum pyridoxal kinase protein for the diagnosis of human and canine visceral leishmaniasis.

João A Oliveira-da-Silva, Amanda S Machado, Fernanda F Ramos, Grasiele S V Tavares, Daniela P Lage, Fernanda Ludolf, Bethina T Steiner, Thiago A R Reis, Thaís T O Santos, Lourena E Costa, Vívian T Martins, Nathália C Galvani, Ana T Chaves, Jamil S Oliveira, Miguel A Chávez-Fumagalli, Danielle F de Magalhães-Soares, Mariana C Duarte, Daniel Menezes-Souza, Julia A G Silveira, Ricardo L F Moreira, Ricardo A Machado-de-Ávila, Unaí Tupinambás, Denise U Gonçalves, Eduardo A F Coelho,

Visceral leishmaniasis (VL) is a highly neglected disease that is present in several countries worldwide. Present-day treatments against this disease are unsuitable, mainly due to the toxicity and/or high cost of drugs. In addition, the development of vaccines is still insufficient. In this scenario, a prompt VL diagnosis was deemed ... Read more >>

Immunol Lett (Immunology letters)
[2020, 220:11-20]

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A Leishmania infantum hypothetical protein evaluated as a recombinant protein and specific B-cell epitope for the serodiagnosis and prognosis of visceral leishmaniasis.

Amanda S Machado, Fernanda F Ramos, João A Oliveira-da-Silva, Thaís T O Santos, Fernanda Ludolf, Grasiele S V Tavares, Lourena E Costa, Daniela P Lage, Bethina T Steiner, Ana T Chaves, Miguel A Chávez-Fumagalli, Danielle F de Magalhães-Soares, Julia A G Silveira, Karina M N Napoles, Unaí Tupinambás, Mariana C Duarte, Ricardo A Machado-de-Ávila, Lílian L Bueno, Ricardo T Fujiwara, Ricardo L F Moreira, Manoel O C Rocha, Rachel B Caligiorne, Eduardo A F Coelho,

The serodiagnosis of visceral leishmaniasis (VL) presents problems related to the sensitivity and/or specificity of the tests. In this context, more refined antigens should be identified and applied for the improvement of disease diagnosis. In the present study, DNA with an encoding of a Leishmania infantum hypothetical protein, LiHyC, was ... Read more >>

Acta Trop (Acta tropica)
[2020, 203:105318]

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Comparison of body composition analysis methods among centenary women: Seeking simpler methods.

Mariana Gf Duarte, Paulo O Duarte, Anderson Pelichek, Eduardo Ferriolli, Julio C Moriguti, Karina Pfrimer, Nereida Kc Lima,

<h4>Objectives</h4>The aim of this study was to evaluate the most commonly used body composition tools in clinical practice, such as anthropometry and electrical bioimpedance, and compare it with deuterium oxide.<h4>Methods</h4>An exploratory cross-sectional study was conducted on women aged 100 years or above at home. Body composition was determined by measuring skinfolds ... Read more >>

SAGE Open Med (SAGE open medicine)
[2019, 7:2050312119865126]

Cited: 1 time

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Immunodiagnosis of human and canine visceral leishmaniasis using recombinant Leishmania infantum Prohibitin protein and a synthetic peptide containing its conformational B-cell epitope.

Marcella R Rodrigues, Lucas M O Santos, Carolina K Miyazaki, Vivian T Martins, Fernanda R Ludolf, Amanda C Kursancew, Fernanda F Ramos, Daniel S Dias, Jamil S Oliveira, Paula M A Vieira, Bruno M Roatt, Ricardo A Machado de Ávila, Denise U Gonçalves, Daniel Menezes-Souza, Eduardo A F Coelho, Mariana C Duarte,

In the present study, Leishmania infantum's Prohibitin was cloned and, alongside a synthetic peptide, evaluated for the serodiagnosis of visceral and tegumentary leishmaniasis (CVL and TL, respectively) in dogs and humans. For TL diagnosis, this study analyzed serum samples from cutaneous (n = 20) or mucosal (n = 39) leishmaniasis patients, and from Chagas ... Read more >>

J Immunol Methods (Journal of immunological methods)
[2019, 474:112641]

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Screening diagnostic candidates from Leishmania infantum proteins for human visceral leishmaniasis using an immunoproteomics approach.

Daniela P Lage, Fernanda Ludolf, Patrícia C Silveira, Amanda S Machado, Fernanda F Ramos, Daniel S Dias, Patrícia A F Ribeiro, Lourena E Costa, Danniele L Vale, Grasiele S V Tavares, Vívian T Martins, Miguel A Chávez-Fumagalli, Rachel B Caligiorne, Ana T Chaves, Denise U Gonçalves, Manoel O C Rocha, Mariana C Duarte, Eduardo A F Coelho,

There is no suitable vaccine against human visceral leishmaniasis (VL) and available drugs are toxic and/or present high cost. In this context, diagnostic tools should be improved for clinical management and epidemiological evaluation of disease. However, the variable sensitivity and/or specificity of the used antigens are limitations, showing the necessity ... Read more >>

Parasitology (Parasitology)
[2019, 146(11):1467-1476]

Cited: 3 times

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A biomarker for tegumentary and visceral leishmaniasis based on a recombinant Leishmania hypothetical protein.

Daniela P Lage, Amanda S Machado, Fernanda F Ramos, Patrícia C Silveira, Daniel S Dias, Patrícia A F Ribeiro, Grasiele S V Tavares, Lourena E Costa, Thaís T O Santos, Bethina T Steiner, Mírian I Fagundes, Miguel A Chávez-Fumagalli, Sandra Lyon, Ricardo L F Moreira, Mariana C Duarte, Daniel Menezes-Souza, Rachel B Caligiorne, Ricardo A Machado-de-Ávila, Antônio L Teixeira, Eduardo A F Coelho,

The measures for leishmaniasis control include the precise diagnosis of disease. However, although several recombinant antigens have been tested with this biotechnological purpose, no effective product exists, which could detects patients with the active disease, as well as differentiates them from cured and treated patients. In this study, a conserved ... Read more >>

Immunobiology (Immunobiology)
[2019, 224(4):477-484]

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Leishmania infantum β-Tubulin Identified by Reverse Engineering Technology through Phage Display Applied as Theranostic Marker for Human Visceral Leishmaniasis.

Lourena E Costa, Patrícia T Alves, Ana Paula Carneiro, Ana C S Dias, Patrícia T Fujimura, Galber R Araujo, Grasiele S V Tavares, Fernanda F Ramos, Mariana C Duarte, Daniel Menezes-Souza, Peter Briza, Fátima F Briza, Eduardo A F Coelho, Luiz Ricardo Goulart,

Two <i>Leishmania infantum</i> mimotopes (B10 and C01) identified by phage display showed to be antigenic and immunogenic for visceral (VL) and tegumentary (TL) leishmaniasis; however, their biological targets in the parasites have not been identified. The aim of the present study was to investigate the native antigens expressing both mimotopes, ... Read more >>

Int J Mol Sci (International journal of molecular sciences)
[2019, 20(8):]

Cited: 4 times

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New antigens for the serological diagnosis of human visceral leishmaniasis identified by immunogenomic screening.

Ana Maria Ravena Severino Carvalho, Tiago Antônio de Oliveira Mendes, Eduardo Antonio Ferraz Coelho, Mariana Costa Duarte, Daniel Menezes-Souza,

Visceral leishmaniasis (VL) still represents a serious public health problem in Brazil due to the inefficiency of the control measures currently employed, that included early diagnosis and treatment of human cases, vector control, euthanasia of infected dogs and, recently approved in Brazil, treatment with Milteforam drug. Effective clinical management depend ... Read more >>

PLoS One (PloS one)
[2018, 13(12):e0209599]

Cited: 4 times

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Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection.

Patrícia A F Ribeiro, Daniel S Dias, Daniela P Lage, Vívian T Martins, Lourena E Costa, Thaís T O Santos, Fernanda F Ramos, Grasiele S V Tavares, Débora V C Mendonça, Fernanda Ludolf, Dawidson A Gomes, Michele A Rodrigues, Miguel A Chávez-Fumagalli, Eduardo S Silva, Alexsandro S Galdino, Mariana C Duarte, Bruno M Roatt, Daniel Menezes-Souza, Antonio L Teixeira, Eduardo A F Coelho,

Vaccination is one the most important strategies for the prevention of visceral leishmaniasis (VL). In the current study, a new Leishmania hypothetical protein, LiHyP, which was previously showed as antigenic in an immunoproteomic search in canine VL, was evaluated regarding its immunogenicity and protective efficacy against Leishmania infantum infection. The ... Read more >>

Mol Immunol (Molecular immunology)
[2019, 106:108-118]

Cited: 3 times

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Exploring Morphine-Triggered PKC-Targets and Their Interaction with Signaling Pathways Leading to Pain via TrkA.

Darlene A Pena, Mariana Lemos Duarte, Dimitrius T Pramio, Lakshmi A Devi, Deborah Schechtman,

It is well accepted that treatment of chronic pain with morphine leads to μ opioid receptor (MOR) desensitization and the development of morphine tolerance. MOR activation by the selective peptide agonist, D-Ala2, N-MePhe4, Gly-ol]-enkephalin(DAMGO), leads to robust G protein receptor kinase activation, β-arrestin recruitment, and subsequent receptor endocytosis, which does ... Read more >>

Proteomes (Proteomes)
[2018, 6(4):]

Cited: 2 times

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Diagnostic application of recombinant Leishmania proteins and evaluation of their in vitro immunogenicity after stimulation of immune cells collected from tegumentary leishmaniasis patients and healthy individuals.

Mariana P Lima, Lourena E Costa, Daniela P Lage, Daniel S Dias, Patrícia A F Ribeiro, Amanda S Machado, Fernanda F Ramos, Beatriz C S Salles, Mirian I Fagundes, Gerusa B Carvalho, Michelle L Franklin, Miguel A Chávez-Fumagalli, Ricardo A Machado-de-Ávila, Daniel Menezes-Souza, Mariana C Duarte, Antonio L Teixeira, Denise U Gonçalves, Eduardo A F Coelho,

The present study evaluated the cytokine profile in PBMC supernatants and the humoral response in mucosal leishmaniasis (ML) patients and in healthy subjects living in an endemic area. Four proteins, which had previously proven to be antigenic in the human disease, were tested: LiHyM, enolase, eukaryotic initiation factor 5a, and ... Read more >>

Cell Immunol (Cellular immunology)
[2018, 334:61-69]

Cited: 1 time

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In vivo antileishmanial efficacy of a naphthoquinone derivate incorporated into a Pluronic® F127-based polymeric micelle system against Leishmania amazonensis infection.

Débora V C Mendonça, Grasiele S V Tavares, Daniela P Lage, Tauane G Soyer, Lívia M Carvalho, Daniel S Dias, Patrícia A F Ribeiro, Flaviano M Ottoni, Luciana M R Antinarelli, Danniele L Vale, Fernanda Ludolf, Mariana C Duarte, Elaine S Coimbra, Miguel A Chávez-Fumagalli, Bruno M Roatt, Daniel Menezes-Souza, José Mário Barichello, Ricardo J Alves, Eduardo A F Coelho,

New therapeutic strategies against leishmaniasis are desirable, since the treatment against disease presents problems, such as the toxicity, high cost and/or parasite resistance. As consequence, new antileishmanial compounds are necessary to be identified, as presenting high activity against Leishmania parasites, but low toxicity in mammalian hosts. Flau-A is a naphthoquinone ... Read more >>

Biomed Pharmacother (Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie)
[2019, 109:779-787]

Cited: 4 times

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A Pluronic® F127-based polymeric micelle system containing an antileishmanial molecule is immunotherapeutic and effective in the treatment against Leishmania amazonensis infection.

Grasiele S V Tavares, Débora V C Mendonça, Carolina K Miyazaki, Daniela P Lage, Tauane G Soyer, Lívia M Carvalho, Flaviano M Ottoni, Daniel S Dias, Patrícia A F Ribeiro, Luciana M R Antinarelli, Fernanda Ludolf, Mariana C Duarte, Elaine S Coimbra, Miguel A Chávez-Fumagalli, Bruno M Roatt, Daniel Menezes-Souza, José Mário Barichello, Ricardo J Alves, Eduardo A F Coelho,

Clioquinol (5-chloro-7-iodoquinolin-8-ol or ICHQ) was recently showed to presents an in vitro effective antileishmanial action, causing changes in membrane permeability, mitochondrial functionality, and parasite morphology. In the present study, ICHQ was incorporated into a Poloxamer 407-based polymeric micelles system (ICHQ/M), and its antileishmanial activity was in vivo evaluated in L. ... Read more >>

Parasitol Int (Parasitology international)
[2019, 68(1):63-72]

Cited: 3 times

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