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Author Margarida Pinto

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Confronting Ceftolozane-Tazobactam Susceptibility in Multidrug-Resistant Enterobacterales Isolates and Whole-Genome Sequencing Results (STEP Study).

Marta Hernández-García, Sergio García-Fernández, María García-Castillo, José Melo-Cristino, Margarida F Pinto, Elsa Gonçalves, Valquíria Alves, Eliana Costa, Elmano Ramalheira, Luísa Sancho, José Diogo, Rui Ferreira, Tânia Silva, Catarina Chaves, Leonor Pássaro, Laura Paixão, João Romano, Rafael Cantón, ,

Ceftolozane-tazobactam (C/T) is frequently used for infections caused by multidrug-resistant (MDR)-Enterobacterales isolates. Whole-genome sequencing (WGS, Illumina-Hiseq 4000/NovaSeq 6000, OGC, UK) was used to study the population structure, the resistome and the virulome of C/T-susceptible and -resistant MDR Escherichia spp. (n=30) and Klebsiella spp. (n=78) isolates, recovered from lower respiratory, intra-abdominal ... Read more >>

Int J Antimicrob Agents (International journal of antimicrobial agents)
[2020, :106259]

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An Eleven-year-old Boy With Arthritis of the Hip Joint, and an 8-year-old Boy With Sacroiliitis.

Tânia Mendo, Susana Norte, Pedro Alves, Delfin Tavares, Margarida Pinto, Catarina Gouveia,

Pediatr Infect Dis J (The Pediatric infectious disease journal)
[2021, Publish Ahead of Print:]

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Distinct epidemiology and resistance mechanisms affecting ceftolozane/tazobactam in Pseudomonas aeruginosa isolates recovered from ICU patients in Spain and Portugal depicted by WGS.

Marta Hernández-García, María García-Castillo, Sergio García-Fernández, José Melo-Cristino, Margarida F Pinto, Elsa Gonçalves, Valquíria Alves, Ana Raquel Vieira, Elmano Ramalheira, Luísa Sancho, José Diogo, Rui Ferreira, Tânia Silva, Catarina Chaves, Germán Bou, Emilia Cercenado, Mercedes Delgado-Valverde, Antonio Oliver, Cristina Pitart, Jesús Rodríguez-Lozano, Nuria Tormo, João Romano, Leonor Pássaro, Laura Paixão, Diego López-Mendoza, Jazmín Díaz-Regañón, Rafael Cantón, ,

<h4>Objectives</h4>To analyse the epidemiology, the resistome and the virulome of ceftolozane/tazobactam-susceptible or -resistant Pseudomonas aeruginosa clinical isolates recovered from surveillance studies in Portugal (STEP, 2017-18) and Spain (SUPERIOR, 2016-17).<h4>Methods</h4>P. aeruginosa isolates were recovered from intra-abdominal, urinary tract and lower respiratory tract infections in ICU patients admitted to 11 Portuguese and ... Read more >>

J Antimicrob Chemother (The Journal of antimicrobial chemotherapy)
[2021, 76(2):370-379]

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INCIDENCE OF URINARY TRACT INFECTION AFTER CYSTOGRAPHY.

Joana Sousa Martins, Margarida Pinto, Manuela Braga, Paulo Calhau,

<h4>Objective</h4>Cystography an invasive procedure with potential complications such as urinary infection (UI). There are few studies about the incidence of complications associated with this procedure. The purpose of this study is to evaluate the incidence of post-cystography urinary infection (UI.).<h4>Methods</h4>Retrospective study with a review of clinical records of patients under ... Read more >>

Rev Paul Pediatr (Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao Paulo)
[2020, 39:e2019386]

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Multidrug-resistant bacteria in diabetic foot infections: Experience from a portuguese tertiary centre.

Tomás Pessoa E Costa, Bruno Duarte, Ana Luísa João, Miguel Coelho, Ana Formiga, Margarida Pinto, José Neves,

In recent years, the emergence of antibiotic resistant pathogens made increasingly difficult to establish appropriate empiric antimicrobial therapy protocols for acute diabetic foot infection (DFI) treatment. Early recognition of the population at-risk for multidrug-resistant (MDR) bacterial infection is of paramount importance in order to decrease large-spectrum antibiotic overuse. This study ... Read more >>

Int Wound J (International wound journal)
[2020, 17(6):1835-1839]

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Fifteen years of a nationwide culture collection of Neisseria gonorrhoeae antimicrobial resistance in Portugal.

Miguel Pinto, João Carlos Rodrigues, Rui Matias, Ivone Água-Doce, Dora Cordeiro, Cristina Correia, João Paulo Gomes, Maria José Borrego, ,

Neisseria gonorrhoeae antimicrobial resistance (AMR) and gonorrhea disease burden remain major public health concerns worldwide. To contribute to the supranational demands to monitor and manage the spread of antimicrobial-resistant N. gonorrhoeae, the Portuguese NIH promoted the creation of the National Laboratory Network for Neisseria gonorrhoeae Collection (PTGonoNet). The present study ... Read more >>

Eur J Clin Microbiol Infect Dis (European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology)
[2020, 39(9):1761-1770]

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In vitro activity of ceftolozane-tazobactam against Enterobacterales and Pseudomonas aeruginosa causing urinary, intra-abdominal and lower respiratory tract infections in intensive care units in Portugal: The STEP multicenter study.

Sergio García-Fernández, María García-Castillo, José Melo-Cristino, Margarida F Pinto, Elsa Gonçalves, Valquíria Alves, Ana Raquel Vieira, Elmano Ramalheira, Luísa Sancho, José Diogo, Rui Ferreira, Daniela Silva, Catarina Chaves, Leonor Pássaro, Laura Paixão, Rafael Cantón, ,

The STEP surveillance study was designed to increase knowledge about distribution of multidrug-resistant (MDR) Enterobacterales and Pseudomonas aeruginosa in Portugal, focusing on the intensive care unit (ICU). Antimicrobial susceptibility of common agents was also evaluated and compared with that of one of the latest therapeutic introductions, ceftolozane-tazobactam (C/T). Clinical isolates ... Read more >>

Int J Antimicrob Agents (International journal of antimicrobial agents)
[2020, 55(3):105887]

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Changes in emm types and superantigen gene content of Streptococcus pyogenes causing invasive infections in Portugal.

A Friães, J Melo-Cristino, M Ramirez, ,

Fluctuations in the clonal composition of Group A Streptococcus (GAS) have been associated with the emergence of successful lineages and with upsurges of invasive infections (iGAS). This study aimed at identifying changes in the clones causing iGAS in Portugal. Antimicrobial susceptibility testing, emm typing and superantigen (SAg) gene profiling were ... Read more >>

Sci Rep (Scientific reports)
[2019, 9(1):18051]

Cited: 2 times

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Diabetic Foot Infection: Causative Pathogens and Empiric Antibiotherapy Considerations-The Experience of a Tertiary Center.

José Miguel Neves, Bruno Duarte, Margarida Pinto, Ana Formiga, José Neves,

Most moderate-to-severe diabetic foot infections (DFIs) require hospitalization with urgent surgical approach and administration of empiric antibiotherapy. To ensure optimal antibiotic coverage, regular microbiological background updates are imperative. The purpose is to characterize the microbiological profile and the antibiotic sensitivity pattern of the DFI causative pathogens isolated within a specialized ... Read more >>

Int J Low Extrem Wounds (The international journal of lower extremity wounds)
[2019, 18(2):122-128]

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Streptococcus canis Are a Single Population Infecting Multiple Animal Hosts Despite the Diversity of the Universally Present M-Like Protein SCM.

Marcos D Pinho, Geoffrey Foster, Constança Pomba, Miguel P Machado, Johanna L Baily, Thijs Kuiken, José Melo-Cristino, Mário Ramirez, ,

<i>Streptococcus canis</i> is an animal pathogen which occasionally causes infections in humans. The <i>S. canis</i> M-like protein (SCM) encoded by the <i>scm</i> gene, is its best characterized virulence factor but previous studies suggested it could be absent in a substantial fraction of isolates. We studied the distribution and variability of ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2019, 10:631]

Cited: 3 times

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Dominance of vaccine serotypes in pediatric invasive pneumococcal infections in Portugal (2012-2015).

Catarina Silva-Costa, Maria J Brito, Sandra I Aguiar, Joana P Lopes, Mário Ramirez, José Melo-Cristino, , ,

We evaluated the impact of continued 13-valent pneumococcal conjugate vaccine (PCV13) use in the private market (uptake of 61%) in pediatric invasive pneumococcal disease (pIPD) in Portugal (2012-2015). The most frequently detected serotypes were: 3 (n = 32, 13.8%), 14 (n = 23, 9.9%), 1 (n = 23, 9.9%), 7F (n = 15, 6.4%), 19A (n = 13, 5.6%), 6B ... Read more >>

Sci Rep (Scientific reports)
[2019, 9(1):6]

Cited: 1 time

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Streptococcus pyogenes Causing Skin and Soft Tissue Infections Are Enriched in the Recently Emerged emm89 Clade 3 and Are Not Associated With Abrogation of CovRS.

Catarina Pato, José Melo-Cristino, Mario Ramirez, Ana Friães, ,

Although skin and soft tissue infections (SSTI) are the most common focal infections associated with invasive disease caused by <i>Streptococcus pyogenes</i> (Lancefield Group A streptococci - GAS), there is scarce information on the characteristics of isolates recovered from SSTI in temperate-climate regions. In this study, 320 GAS isolated from SSTI ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2018, 9:2372]

Cited: 1 time

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Pediatric Complicated Pneumonia Caused by Streptococcus pneumoniae Serotype 3 in 13-Valent Pneumococcal Conjugate Vaccinees, Portugal, 2010-2015.

Catarina Silva-Costa, Maria João Brito, Marcos D Pinho, Ana Friães, Sandra I Aguiar, M Ramirez, Jose Melo-Cristino, , ,

Despite use of 7-valent pneumococcal conjugate vaccine, incidence of pleural effusion and empyema (pediatric complicated pneumococcal pneumonia [PCPP]) is reportedly increasing globally. We cultured and performed PCR on 152 pleural fluid samples recovered from pediatric patients in Portugal during 2010-2015 to identify and serotype Streptococcus pneumoniae. We identified only 17 ... Read more >>

Emerg Infect Dis (Emerging infectious diseases)
[2018, 24(7):1307-1314]

Cited: 15 times

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Increasing macrolide resistance among Streptococcus agalactiae causing invasive disease in non-pregnant adults was driven by a single capsular-transformed lineage, Portugal, 2009 to 2015.

Elísia Lopes, Tânia Fernandes, Miguel P Machado, João André Carriço, José Melo-Cristino, Mário Ramirez, Elisabete R Martins, ,

We characterised Lancefield group B streptococcal (GBS) isolates causing invasive disease among non-pregnant adults in Portugal between 2009 and 2015. All isolates (n = 555) were serotyped, assigned to clonal complexes (CCs) by multilocus sequence typing and characterised by surface protein and pilus island gene profiling. Antimicrobial susceptibility was tested by disk ... Read more >>

Euro Surveill (Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin)
[2018, 23(21):]

Cited: 3 times

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Pasteurella infections in a tertiary centre - from cellulitis to multiple-organ failure: Retrospective case series.

Zsófia Vesza, Matteo Boattini, Margarida Pinto, Pedro Marques da Silva,

The purpose of this article is to review the clinical features of pasteurellosis in a tertiary centre over a period of 4½ years. We have identified eight cases of Pasteurella multocida and one case of Pasteurella canis infection, with a large diversity of clinical pictures and outcomes. All patients were ... Read more >>

SAGE Open Med Case Rep (SAGE open medical case reports)
[2017, 5:2050313X17748286]

Cited: 0 times

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Streptococcus agalactiae Causing Neonatal Infections in Portugal (2005-2015): Diversification and Emergence of a CC17/PI-2b Multidrug Resistant Sublineage.

Elisabete R Martins, Cristiano Pedroso-Roussado, José Melo-Cristino, Mário Ramirez, ,

The molecular characterization of 218 GBS isolates recovered from neonatal invasive infections in Portugal in 2005-2015 revealed the existence of a small number of genetically distinct lineages that were present over a significant time-span. Serotypes III and Ia were dominant in the population, together accounting for >80% of the isolates. ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2017, 8:499]

Cited: 12 times

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Serotype 3 Remains the Leading Cause of Invasive Pneumococcal Disease in Adults in Portugal (2012-2014) Despite Continued Reductions in Other 13-Valent Conjugate Vaccine Serotypes.

Andreia N Horácio, Catarina Silva-Costa, Joana P Lopes, Mário Ramirez, José Melo-Cristino, ,

Since 2010 the 13-valent pneumococcal conjugate vaccine (PCV13) replaced the 7-valent vaccine (PCV7) as the leading pneumococcal vaccine used in children through the private sector. Although, neither of the PCVs were used significantly in adults, changes in adult invasive pneumococcal disease (IPD) were expected due to herd protection. We characterized ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2016, 7:1616]

Cited: 21 times

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Infections on the rise: Raoultella spp., clinical and microbiological findings from a retrospective study, 2010-2014.

Matteo Boattini, André Almeida, Catarina Cardoso, Cristiano Silva Cruz, Catarina Machado, Zsófia Vesza, Valentina Tosatto, Dionísio Maia, Sara Cardoso, Margarida Pinto, Rita Barata Moura, Teresa Garcia, António Sousa Guerreiro,

We performed a retrospective analysis of clinical and laboratory data over 5 years in a tertiary centre to assess clinical and microbiological characteristics of patients with Raoultella spp. infection. Raoultella spp. were deemed responsible for clinical infections in 57 patients (R. planticola, n = 32 and R. ornithinolytica, n = ... Read more >>

Infect Dis (Lond) (Infectious diseases (London, England))
[2016, 48(1):87-91]

Cited: 15 times

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Two novel CMY-2-type β-lactamases encountered in clinical Escherichia coli isolates.

Vera Manageiro, Eugénia Ferreira, Margarida Pinto, Fernando Fonseca, Mónica Ferreira, Richard Bonnet, Manuela Caniça,

<h4>Background</h4>Chromosomally encoded AmpC β-lactamases may be acquired by transmissible plasmids which consequently can disseminate into bacteria lacking or poorly expressing a chromosomal bla AmpC gene. Nowadays, these plasmid-mediated AmpC β-lactamases are found in different bacterial species, namely Enterobacteriaceae, which typically do not express these types of β-lactamase such as Klebsiella ... Read more >>

Ann Clin Microbiol Antimicrob (Annals of clinical microbiology and antimicrobials)
[2015, 14:12]

Cited: 2 times

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Declining macrolide resistance in Streptococcus pyogenes in Portugal (2007-13) was accompanied by continuous clonal changes.

C Silva-Costa, M Ramirez, J Melo-Cristino, ,

<h4>Objectives</h4>Macrolide resistance among Streptococcus pyogenes [group A streptococci (GAS)] in Portugal decreased between 1999 and 2006 and this decrease was accompanied by alterations in the prevalence of macrolide resistance phenotypes and clonal composition of the population. The aims of this study were to determine the macrolide resistance rate, resistance phenotypes ... Read more >>

J Antimicrob Chemother (The Journal of antimicrobial chemotherapy)
[2015, 70(10):2729-2733]

Cited: 8 times

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Genetic and phenotypic characterization of Listeria monocytogenes from human clinical cases that occurred in Portugal between 2008 and 2012.

Rui Magalhães, Vânia Ferreira, Isabel Santos, Gonçalo Almeida, Paula Teixeira, ,

Listeria monocytogenes infection (listeriosis) is an uncommon but severe foodborne illness that affects mainly individuals with recognized underlying conditions: the elderly, immunocompromised individuals, and pregnant women and their fetuses. The aim of this study was to obtain epidemiological data on cases of listeriosis occurring in Portugal from 2008 through 2012, ... Read more >>

Foodborne Pathog Dis (Foodborne pathogens and disease)
[2014, 11(11):907-916]

Cited: 7 times

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First description of OXA-48 carbapenemase harbored by Escherichia coli and Enterobacter cloacae from a single patient in Portugal.

Vera Manageiro, Eugénia Ferreira, Margarida Pinto, Manuela Caniça,

Antimicrob Agents Chemother (Antimicrobial agents and chemotherapy)
[2014, 58(12):7613-7614]

Cited: 9 times

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Non-invasive pneumococcal pneumonia in Portugal--serotype distribution and antimicrobial resistance.

Andreia N Horácio, Joana P Lopes, Mário Ramirez, José Melo-Cristino, ,

There is limited information on the serotypes causing non-invasive pneumococcal pneumonia (NIPP). Our aim was to characterize pneumococci causing NIPP in adults to determine recent changes in serotype prevalence, the potential coverage of pneumococcal vaccines and changes in antimicrobial resistance. Serotypes and antimicrobial susceptibility profiles of a sample of 1300 ... Read more >>

PLoS One (PloS one)
[2014, 9(7):e103092]

Cited: 6 times

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Massive dissemination of methicillin resistant Staphylococcus aureus in bloodstream infections in a high MRSA prevalence country: establishment and diversification of EMRSA-15.

Nuno A Faria, Maria Miragaia, Hermínia de Lencastre, ,

Portugal is the European country with the highest prevalence of methicillin resistant Staphylococcus aureus (MRSA), in which EMRSA-15 (ST22-IVh) has been the dominant clone since soon after its introduction in Portuguese hospitals in 2001. In this study, we intend to not only, assess the evolution of the invasive MRSA in ... Read more >>

Microb Drug Resist (Microbial drug resistance (Larchmont, N.Y.))
[2013, 19(6):483-490]

Cited: 6 times

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The majority of adult pneumococcal invasive infections in Portugal are still potentially vaccine preventable in spite of significant declines of serotypes 1 and 5.

Andreia N Horácio, Jorge Diamantino-Miranda, Sandra I Aguiar, Mário Ramirez, José Melo-Cristino, ,

In Portugal, pneumococcal conjugate vaccines have been administered to children outside of the national immunization plan since 2001. We determined the serotype and antimicrobial susceptibility of 1265 isolates responsible for adult invasive pneumococcal infections (IPD) between 2009 and 2011 and compared the results with previously published data from 1999 to ... Read more >>

PLoS One (PloS one)
[2013, 8(9):e73704]

Cited: 11 times

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