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Author Mami Matano

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Wnt Signaling Shapes the Histological Variation in Diffuse Gastric Cancer.

Kazuhiro Togasaki, Shinya Sugimoto, Yuki Ohta, Kosaku Nanki, Mami Matano, Sirirat Takahashi, Masayuki Fujii, Takanori Kanai, Toshiro Sato,

BACKGROUND & AIMS:Diffuse type gastric cancer is currently subdivided into signet-ring cell carcinoma (SRCC) and non-SRCC referred to as poorly cohesive carcinoma not otherwise specified (PCC-NOS). Although these subtypes are considered to be independent, they often co-exist in the same tumors, raising a question whether they clonally differ or not. ... Read more >>

Gastroenterology (Gastroenterology)
[2020, :]

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An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping.

Kenta Kawasaki, Kohta Toshimitsu, Mami Matano, Masashi Fujita, Masayuki Fujii, Kazuhiro Togasaki, Toshiki Ebisudani, Mariko Shimokawa, Ai Takano, Sirirat Takahashi, Yuki Ohta, Kosaku Nanki, Ryo Igarashi, Kazuhiro Ishimaru, Hiroki Ishida, Yasutaka Sukawa, Shinya Sugimoto, Yoshimasa Saito, Kazuhiro Maejima, Shota Sasagawa, Hwajin Lee, Hong-Gee Kim, Kyungsik Ha, Junko Hamamoto, Koichi Fukunaga, Aya Maekawa, Minoru Tanabe, Soichiro Ishihara, Yasuo Hamamoto, Hiroyuki Yasuda, Shigeki Sekine, Atsushi Kudo, Yuko Kitagawa, Takanori Kanai, Hidewaki Nakagawa, Toshiro Sato,

Gastroenteropancreatic (GEP) neuroendocrine neoplasm (NEN) that consists of neuroendocrine tumor and neuroendocrine carcinoma (NEC) is a lethal but under-investigated disease owing to its rarity. To fill the scarcity of clinically relevant models of GEP-NEN, we here established 25 lines of NEN organoids and performed their comprehensive molecular characterization. GEP-NEN organoids ... Read more >>

Cell (Cell)
[2020, 183(5):1420-1435.e21]

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LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium.

Rosalie T Zwiggelaar, Håvard T Lindholm, Madeleine Fosslie, Marianne Terndrup Pedersen, Yuki Ohta, Alberto Díez-Sánchez, Mara Martín-Alonso, Jenny Ostrop, Mami Matano, Naveen Parmar, Emilie Kvaløy, Roos R Spanjers, Kamran Nazmi, Morten Rye, Finn Drabløs, Cheryl Arrowsmith, John Arne Dahl, Kim B Jensen, Toshiro Sato, Menno J Oudhoff,

Intestinal epithelial homeostasis is maintained by adult intestinal stem cells, which, alongside Paneth cells, appear after birth in the neonatal period. We aimed to identify regulators of neonatal intestinal epithelial development by testing a small library of epigenetic modifier inhibitors in Paneth cell-skewed organoid cultures. We found that lysine-specific demethylase ... Read more >>

Sci Adv (Science advances)
[2020, 6(37):]

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Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids.

Sayoko Ito-Harashima, Mami Matano, Kana Onishi, Tomofumi Nomura, Saki Nakajima, Shingo Ebata, Kazuhiro Shiizaki, Masanobu Kawanishi, Takashi Yagi,

Background:Sex steroid hormone receptors are classified into three classes of receptors: estrogen receptors (ER) α and β, androgen receptor (AR), and progesterone receptor (PR). They belong to the nuclear receptor superfamily and activate their downstream genes in a ligand-dependent manner. Since sex steroid hormones are involved in a wide variety ... Read more >>

Genes Environ (Genes and environment : the official journal of the Japanese Environmental Mutagen Society)
[2020, 42:20]

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LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium

Rosalie Zwiggelaar, Håvard Lindholm, Madeleine Fosslie, Marianne Pedersen, Yuki Ohta, Alberto Díez-Sánchez, Mara Martín-Alonso, Jenny Ostrop, Mami Matano, Naveen Parmar, Emilie Kvaløy, Roos Spanjers, Kamran Nazmi, Morten Rye, Finn Drabløs, Cheryl Arrowsmith, John Arne Dahl, Kim Jensen, Toshiro Sato, Menno Oudhoff,

ABSTRACT Intestinal epithelial homeostasis is maintained by adult intestinal stem cells, which, alongside Paneth cells, appear after birth in the neonatal period. We aimed to identify new regulators of neonatal intestinal epithelial development by testing a small library of epigenetic modifier inhibitors in Paneth cell-skewed organoid cultures. We found that ... Read more >>

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Somatic inflammatory gene mutations in human ulcerative colitis epithelium.

Kosaku Nanki, Masayuki Fujii, Mariko Shimokawa, Mami Matano, Shingo Nishikori, Shoichi Date, Ai Takano, Kohta Toshimitsu, Yuki Ohta, Sirirat Takahashi, Shinya Sugimoto, Kazuhiro Ishimaru, Kenta Kawasaki, Yoko Nagai, Ryota Ishii, Kosuke Yoshida, Nobuo Sasaki, Toshifumi Hibi, Soichiro Ishihara, Takanori Kanai, Toshiro Sato,

With ageing, normal human tissues experience an expansion of somatic clones that carry cancer mutations1-7. However, whether such clonal expansion exists in the non-neoplastic intestine remains unknown. Here, using whole-exome sequencing data from 76 clonal human colon organoids, we identify a unique pattern of somatic mutagenesis in the inflamed epithelium ... Read more >>

Nature (Nature)
[2020, 577(7789):254-259]

Cited: 6 times

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Chromosome Engineering of Human Colon-Derived Organoids to Develop a Model of Traditional Serrated Adenoma.

Kenta Kawasaki, Masayuki Fujii, Shinya Sugimoto, Keiko Ishikawa, Mami Matano, Yuki Ohta, Kohta Toshimitsu, Sirirat Takahashi, Naoki Hosoe, Shigeki Sekine, Takanori Kanai, Toshiro Sato,

BACKGROUND & AIMS:Traditional serrated adenomas (TSAs) are rare colorectal polyps with unique histologic features. Fusions in R-spondin genes have been found in TSAs, but it is not clear whether these are sufficient for TSA development, due to the lack of a chromosome engineering platform for human tissues. We studied the ... Read more >>

Gastroenterology (Gastroenterology)
[2020, 158(3):638-651.e8]

Cited: 1 time

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Author Correction: Efficient genetic engineering of human intestinal organoids using electroporation.

Masayuki Fujii, Mami Matano, Kosaku Nanki, Toshiro Sato,

The version of this paper originally published shows incorrect units for two plasmid concentrations. In the "Reagent Setup" section, the instructions for sgRNA-Cas9 plasmid should read "Adjust the concentration of each plasmid to 1 μg μl-1," rather than "to 1 μg ml-1." Similarly, all concentrations in the tables in Steps ... Read more >>

Nat Protoc (Nature protocols)
[2019, 14(8):2595]

Cited: 0 times

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Human Intestinal Organoids Maintain Self-Renewal Capacity and Cellular Diversity in Niche-Inspired Culture Condition.

Masayuki Fujii, Mami Matano, Kohta Toshimitsu, Ai Takano, Yohei Mikami, Shingo Nishikori, Shinya Sugimoto, Toshiro Sato,

Cellular diversity that shapes tissue architecture and function is governed by multiple niche signals. Nonetheless, maintaining cellular diversity in human intestinal organoids has been challenging. Based on niche ligands present in the natural stem cell milieu, we establish a refined organoid culture condition for intestinal epithelia that allows human intestinal ... Read more >>

Cell Stem Cell (Cell stem cell)
[2018, 23(6):787-793.e6]

Cited: 22 times

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Divergent Routes toward Wnt and R-spondin Niche Independency during Human Gastric Carcinogenesis.

Kosaku Nanki, Kohta Toshimitsu, Ai Takano, Masayuki Fujii, Mariko Shimokawa, Yuki Ohta, Mami Matano, Takashi Seino, Shingo Nishikori, Keiko Ishikawa, Kenta Kawasaki, Kazuhiro Togasaki, Sirirat Takahashi, Yasutaka Sukawa, Hiroki Ishida, Shinya Sugimoto, Hirofumi Kawakubo, Jihoon Kim, Yuko Kitagawa, Shigeki Sekine, Bon-Kyoung Koo, Takanori Kanai, Toshiro Sato,

Recent sequencing analyses have shed light on heterogeneous patterns of genomic aberrations in human gastric cancers (GCs). To explore how individual genetic events translate into cancer phenotypes, we established a biological library consisting of genetically engineered gastric organoids carrying various GC mutations and 37 patient-derived organoid lines, including rare genomically ... Read more >>

Cell (Cell)
[2018, 174(4):856-869.e17]

Cited: 25 times

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Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression.

Takashi Seino, Shintaro Kawasaki, Mariko Shimokawa, Hiroki Tamagawa, Kohta Toshimitsu, Masayuki Fujii, Yuki Ohta, Mami Matano, Kosaku Nanki, Kenta Kawasaki, Sirirat Takahashi, Shinya Sugimoto, Eisuke Iwasaki, Junichi Takagi, Takao Itoi, Minoru Kitago, Yuko Kitagawa, Takanori Kanai, Toshiro Sato,

Despite recent efforts to dissect the inter-tumor heterogeneity of pancreatic ductal adenocarcinoma (PDAC) by determining prognosis-predictive gene expression signatures for specific subtypes, their functional differences remain elusive. Here, we established a pancreatic tumor organoid library encompassing 39 patient-derived PDACs and identified 3 functional subtypes based on their stem cell niche ... Read more >>

Cell Stem Cell (Cell stem cell)
[2018, 22(3):454-467.e6]

Cited: 61 times

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Reconstruction of the Human Colon Epithelium In Vivo.

Shinya Sugimoto, Yuki Ohta, Masayuki Fujii, Mami Matano, Mariko Shimokawa, Kosaku Nanki, Shoichi Date, Shingo Nishikori, Yoshihiro Nakazato, Tetsuya Nakamura, Takanori Kanai, Toshiro Sato,

Genetic lineage tracing has revealed that Lgr5+ murine colon stem cells (CoSCs) rapidly proliferate at the crypt bottom. However, the spatiotemporal dynamics of human CoSCs in vivo have remained experimentally intractable. Here we established an orthotopic xenograft system for normal human colon organoids, enabling stable reconstruction of the human colon epithelium ... Read more >>

Cell Stem Cell (Cell stem cell)
[2018, 22(2):171-176.e5]

Cited: 17 times

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Visualization and targeting of LGR5+ human colon cancer stem cells.

Mariko Shimokawa, Yuki Ohta, Shingo Nishikori, Mami Matano, Ai Takano, Masayuki Fujii, Shoichi Date, Shinya Sugimoto, Takanori Kanai, Toshiro Sato,

The cancer stem cell (CSC) theory highlights a self-renewing subpopulation of cancer cells that fuels tumour growth. The existence of human CSCs is mainly supported by xenotransplantation of prospectively isolated cells, but their clonal dynamics and plasticity remain unclear. Here, we show that human LGR5+ colorectal cancer cells serve as ... Read more >>

Nature (Nature)
[2017, 545(7653):187-192]

Cited: 123 times

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A Colorectal Tumor Organoid Library Demonstrates Progressive Loss of Niche Factor Requirements during Tumorigenesis.

Masayuki Fujii, Mariko Shimokawa, Shoichi Date, Ai Takano, Mami Matano, Kosaku Nanki, Yuki Ohta, Kohta Toshimitsu, Yoshihiro Nakazato, Kenta Kawasaki, Toshio Uraoka, Toshiaki Watanabe, Takanori Kanai, Toshiro Sato,

Colorectal tumor is a heterogeneous disease, with varying clinical presentation and prognosis in patients. To establish a platform encompassing this diversity, we generated 55 colorectal tumor organoid lines from a range of histological subtypes and clinical stages, including rare subtypes. Each line was defined by gene expression signatures and optimized for organoid ... Read more >>

Cell Stem Cell (Cell stem cell)
[2016, 18(6):827-838]

Cited: 135 times

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Efficient genetic engineering of human intestinal organoids using electroporation.

Masayuki Fujii, Mami Matano, Kosaku Nanki, Toshiro Sato,

Gene modification in untransformed human intestinal cells is an attractive approach for studying gene function in intestinal diseases. However, because of the lack of practical tools, such studies have largely depended upon surrogates, such as gene-engineered mice or immortalized human cell lines. By taking advantage of the recently developed intestinal ... Read more >>

Nat Protoc (Nature protocols)
[2015, 10(10):1474-1485]

Cited: 54 times

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Sweetness characterization of recombinant human lysozyme.

Mami Matano, Kana Nakajima, Yutaka Kashiwagi, Shigezo Udaka, Kenji Maehashi,

Lysozyme, a bacteriolytic enzyme, is widely distributed in nature and is a component of the innate immune system. It is established that chicken egg lysozyme elicits sweetness. However, the sweetness of human milk lysozyme, which is vital for combating microbial infections of the gastrointestinal tract of breast-fed infants, has not ... Read more >>

Comp Biochem Physiol B Biochem Mol Biol (Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology)
[2015, 188:8-14]

Cited: 1 time

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Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids.

Mami Matano, Shoichi Date, Mariko Shimokawa, Ai Takano, Masayuki Fujii, Yuki Ohta, Toshiaki Watanabe, Takanori Kanai, Toshiro Sato,

Human colorectal tumors bear recurrent mutations in genes encoding proteins operative in the WNT, MAPK, TGF-β, TP53 and PI3K pathways. Although these pathways influence intestinal stem cell niche signaling, the extent to which mutations in these pathways contribute to human colorectal carcinogenesis remains unclear. Here we use the CRISPR-Cas9 genome-editing ... Read more >>

Nat. Med. (Nature medicine)
[2015, 21(3):256-262]

Cited: 291 times

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Cross-talk between RORγt+ innate lymphoid cells and intestinal macrophages induces mucosal IL-22 production in Crohn's disease.

Shinta Mizuno, Yohei Mikami, Nobuhiko Kamada, Tango Handa, Atsushi Hayashi, Toshiro Sato, Katsuyoshi Matsuoka, Mami Matano, Yuki Ohta, Akira Sugita, Kazutaka Koganei, Rikisaburo Sahara, Masakazu Takazoe, Tadakazu Hisamatsu, Takanori Kanai,

Interleukin (IL)-22-producing RORγt innate lymphoid cells (ILCs) play a pivotal role in intestinal immunity. Recent reports demonstrated that ILCs contribute to mucosal protection and intestinal inflammation in mice. In humans, numbers of RORγt ILCs are significantly increased in the intestine of patients with Crohn's disease (CD), suggesting that ILCs may ... Read more >>

Inflamm. Bowel Dis. (Inflammatory bowel diseases)
[2014, 20(8):1426-1434]

Cited: 30 times

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Biochemical and functional characterization of transiently expressed in neural precursor (TENP) protein in emu egg white.

Kenji Maehashi, Megumi Ueda, Mami Matano, Junko Takeuchi, Masataka Uchino, Yutaka Kashiwagi, Toshihiro Watanabe,

A protein transiently expressed in the neural precursors of developing tissues (TENP) was found to be present in emu (Dromaius novaehollandiae) egg white as one of the major proteins. Nucleotide analysis of its encoding cDNA revealed a sequence of 452 amino acids including a 19 amino acid peptide signal. Phylogenetic ... Read more >>

J Agric Food Chem (Journal of agricultural and food chemistry)
[2014, 62(22):5156-5162]

Cited: 5 times

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Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white.

Kenji Maehashi, Mami Matano, Tomohiro Irisawa, Masataka Uchino, Yutaka Kashiwagi, Toshihiro Watanabe,

Lysozyme (LZ), a bacteriolytic enzyme, is found in the egg white of many avian eggs and plays an important role in host defense; however, LZ activity in emu (Dromaius novaehollandiae) egg white is exceptionally undetectable. We cloned and characterized emu goose-type LZ (LZG) and chicken-type LZ (LZC) genes. RT-PCR analysis ... Read more >>

Gene (Gene)
[2012, 492(1):244-249]

Cited: 3 times

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Primary structure of potential allergenic proteins in emu (Dromaius novaehollandiae) egg white.

Kenji Maehashi, Mami Matano, Tomohiro Irisawa, Masataka Uchino, Yasuharu Itagaki, Katsumi Takano, Yutaka Kashiwagi, Toshihiro Watanabe,

The emu (Dromaius novaehollandiae) egg is considered promising as an alternative egg product. To obtain basic biochemical information on emu egg white, the major protein compositions in emu and chicken egg whites and the primary structures of potential allergenic proteins were compared. The dominant protein in emu egg white was ... Read more >>

J Agric Food Chem (Journal of agricultural and food chemistry)
[2010, 58(23):12530-12536]

Cited: 3 times

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Extracellular production of riboflavin-binding protein, a potential bitter inhibitor, by Brevibacillus choshinensis.

Kenji Maehashi, Mami Matano, Makiko Saito, Shigezo Udaka,

Riboflavin-binding protein (RBP) is a glycophosphoprotein found in hen eggs. We previously identified the extraordinary characteristic of RBP in reducing bitterness. For a more detailed study on the mode of action and industrial application of this characteristic, we investigated the microbial production of recombinant RBP (rRBP). We constructed a chicken ... Read more >>

Protein Expr Purif (Protein expression and purification)
[2010, 71(1):85-90]

Cited: 6 times

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The primary structure of a novel riboflavin-binding protein of emu (Dromaius novaehollandiae).

Kenji Maehashi, Mami Matano, Masataka Uchino, Yasushi Yamamoto, Katsumi Takano, Toshihiro Watanabe,

Emu riboflavin-binding protein (RBP) was purified from egg white and yolk, and its N-terminal amino acid sequence was determined. The molecular mass of emu RBP was estimated at approximately 48 and 45 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, i.e., 10 kDa larger than chicken RBP. The molecular mass of ... Read more >>

Comp. Biochem. Physiol. B, Biochem. Mol. Biol. (Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology)
[2009, 153(1):95-100]

Cited: 3 times

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Riboflavin-binding protein is a novel bitter inhibitor.

Kenji Maehashi, Mami Matano, Miho Nonaka, Shigezo Udaka, Yasushi Yamamoto,

Riboflavin-binding protein (RBP) from chicken egg, which was recently reported to be a selective sweet inhibitor for protein sweeteners, was also found to be a bitter inhibitor. RBP elicited broadly tuned inhibition of various bitter substances including quinine-HCl, naringin, theobromine, caffeine, glycyl-L-phenylalanine (Gly-Phe), and denatonium benzoate, whereas several other proteins, ... Read more >>

Chem. Senses (Chemical senses)
[2008, 33(1):57-63]

Cited: 7 times

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Bitter peptides activate hTAS2Rs, the human bitter receptors.

Kenji Maehashi, Mami Matano, Hong Wang, Lynn A Vo, Yasushi Yamamoto, Liquan Huang,

Fermented food contains numerous peptides derived from material proteins. Bitter peptides formed during the fermentation process are responsible for the bitter taste of fermented food. We investigated whether human bitter receptors (hTAS2Rs) recognize bitterness of peptides with a heterologous expression system. HEK293 cells expressing hTAS2R1, hTAS2R4, hTAS2R14, and hTAS2R16 responded ... Read more >>

Biochem Biophys Res Commun (Biochemical and biophysical research communications)
[2008, 365(4):851-855]

Cited: 33 times

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