Full Text Journal Articles by
Author Lex E X Leong

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Whole-Genome Sequencing of SARS-CoV-2 from Quarantine Hotel Outbreak.

Lex E X Leong, Julien Soubrier, Mark Turra, Emma Denehy, Luke Walters, Karin Kassahn, Geoff Higgins, Tom Dodd, Robert Hall, Katina D'Onise, Nicola Spurrier, Ivan Bastian, Chuan K Lim,

Hotel quarantine for international travelers has been used to prevent coronavirus disease spread into Australia. A quarantine hotel-associated community outbreak was detected in South Australia. Real-time genomic sequencing enabled rapid confirmation tracking the outbreak to a recently returned traveler and linked 2 cases of infection in travelers at the same ... Read more >>

Emerg Infect Dis (Emerging infectious diseases)
[2021, 27(8):2219-2221]

Cited: 0 times

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Impact of Meningococcal B (4CMenB) Vaccine on Pharyngeal Neisseria meningitidis Carriage Density and Persistence in Adolescents.

Mark McMillan, Luke Walters, Thomas Sullivan, Lex E X Leong, Mark Turra, Andrew Lawrence, Ann P Koehler, Adam Finn, Ross M Andrews, Helen S Marshall,

<h4>Background</h4>Higher density of Neisseria meningitidis carriage may be associated with transmission of the meningococcus. Our aim was to establish the impact of meningococcal B (4CMenB) vaccine on N. meningitidis carriage density.<h4>Methods</h4>We compared 4CMenB vaccine to control among 913 South Australian students aged approximately 15-18 years in a cluster randomized trial ... Read more >>

Clin Infect Dis (Clinical infectious diseases : an official publication of the Infectious Diseases Society of America)
[2021, 73(1):e99-e106]

Cited: 1 time

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Intra-abdominal infection with Campylobacter curvus: case report and review of the literature.

Patrick C K Tam, Lex E X Leong, Maria Theodossi, David L Gordon,

<h4>Background</h4><i>Campylobacter curvus</i> is a Gram-negative bacteria associated with periodontal disease in humans. Cases of extra-oral manifestations of infection are rare with only six reported cases of extra-oral infection including this report that have been identified in the current literature. Molecular methods are generally used to identify <i>C. curvus</i> while optimal ... Read more >>

Access Microbiol (Access microbiology)
[2021, 3(4):000227]

Cited: 0 times

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The cystic fibrosis gut as a potential source of multidrug resistant pathogens.

Steven L Taylor, Lex E X Leong, Sarah K Sims, Rebecca L Keating, Lito E Papanicolas, Alyson Richard, Fredrick M Mobegi, Steve Wesselingh, Lucy D Burr, Geraint B Rogers,

<h4>Background</h4>The emergence of multidrug resistant (MDR) pathogens represents a profound threat to global health. Individuals with CF have amongst the highest cumulative antibiotic exposure of any patient group, including to critically-important last-line agents. While there is little evidence that antibiotic resistance in airway pathogens results in worse clinical outcomes for ... Read more >>

J Cyst Fibros (Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society)
[2021, 20(3):413-420]

Cited: 0 times

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Total bacterial load, inflammation, and structural lung disease in paediatric cystic fibrosis.

Steven L Taylor, Lex E X Leong, Kerry L Ivey, Steve Wesselingh, Keith Grimwood, Claire E Wainwright, Geraint B Rogers, ,

<h4>Background</h4>Cystic fibrosis (CF) is characterised by reduced airway clearance, microbial accumulation, inflammation, and lung function decline. Certain bacterial species may contribute disproportionately to worsening lung disease. However, the relative importance of these microorganisms compared to the absolute abundance of all bacteria is uncertain. We aimed to identify the characteristics of ... Read more >>

J Cyst Fibros (Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society)
[2020, 19(6):923-930]

Cited: 2 times

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Changes in the Composition of the Gut Microbiota and the Blood Transcriptome in Preterm Infants at Less than 29 Weeks Gestation Diagnosed with Bronchopulmonary Dysplasia.

Feargal J Ryan, Damian P Drew, Chloe Douglas, Lex E X Leong, Max Moldovan, Miriam Lynn, Naomi Fink, Anastasia Sribnaia, Irmeli Penttila, Andrew J McPhee, Carmel T Collins, Maria Makrides, Robert A Gibson, Geraint B Rogers, David J Lynn,

Bronchopulmonary dysplasia (BPD) is a common chronic lung condition in preterm infants that results in abnormal lung development and leads to considerable morbidity and mortality, making BPD one of the most common complications of preterm birth. We employed RNA sequencing and 16S rRNA gene sequencing to profile gene expression in ... Read more >>

mSystems (mSystems)
[2019, 4(5):]

Cited: 5 times

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Soluble fibre supplementation with and without a probiotic in adults with asthma: A 7-day randomised, double blind, three way cross-over trial.

Rebecca McLoughlin, Bronwyn S Berthon, Geraint B Rogers, Katherine J Baines, Lex E X Leong, Peter G Gibson, Evan J Williams, Lisa G Wood,

<h4>Background</h4>Soluble fibre modulates airway inflammation in animal models. The aim of this study was to investigate the effects of soluble fibre supplementation, with and without a probiotic, on plasma short chain fatty acids (SCFA), airway inflammation, asthma control and gut microbiome in adults with asthma.<h4>Methods</h4>A randomised, double-blinded, placebo controlled 3-way ... Read more >>

EBioMedicine (EBioMedicine)
[2019, 46:473-485]

Cited: 7 times

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Examining the Evidence for an Adult Healthy Middle Ear Microbiome.

Jake Jervis-Bardy, Lex E X Leong, Lito E Papanicolas, Kerry L Ivey, Sharad Chawla, Charmaine M Woods, Claire Frauenfelder, Eng H Ooi, Geraint B Rogers,

Otitis media (OM) is a cluster of diseases of the middle ear that commonly result from bacterial infection. OM subtypes in which the tympanic membrane is intact (acute otitis media and otitis media with effusion) are presumed to result from pathogen translocation through the eustachian tube. Recent molecular-based studies have ... Read more >>

mSphere (mSphere)
[2019, 4(5):]

Cited: 1 time

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The Capacity of the Fecal Microbiota From Malawian Infants to Ferment Resistant Starch.

Yanan Wang, Elissa K Mortimer, Kondwani G H Katundu, Noel Kalanga, Lex E X Leong, Geetha L Gopalsamy, Claus T Christophersen, Alyson C Richard, Aravind Shivasami, Guy C J Abell, Graeme P Young, Geraint B Rogers,

In Low and Middle-Income Countries (LMIC), weaning is associated with environmentally acquired and inflammation-associated enteric disorders. Dietary intake of high amylose maize starch (HAMS) can promote commensal fermentative bacteria and drive the production of short chain fatty acids (SCFAs). By stabilizing commensal gut microbiology, and stimulating the production of anti-inflammatory ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2019, 10:1459]

Cited: 0 times

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Long-Term Azithromycin Reduces Haemophilus influenzae and Increases Antibiotic Resistance in Severe Asthma.

Steven L Taylor, Lex E X Leong, Fredrick M Mobegi, Jocelyn M Choo, Steve Wesselingh, Ian A Yang, John W Upham, Paul N Reynolds, Sandra Hodge, Alan L James, Christine Jenkins, Matthew J Peters, Melissa Baraket, Guy B Marks, Peter G Gibson, Geraint B Rogers, Jodie L Simpson,

<b>Rationale:</b> The macrolide antibiotic azithromycin reduces exacerbations in adults with persistent symptomatic asthma. However, owing to the pleotropic properties of macrolides, unintended bacteriological consequences such as augmented pathogen colonization or dissemination of antibiotic-resistant organisms can occur, calling into question the long-term safety of azithromycin maintenance therapy.<b>Objectives:</b> To assess the effects ... Read more >>

Am J Respir Crit Care Med (American journal of respiratory and critical care medicine)
[2019, 200(3):309-317]

Cited: 18 times

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Case report: Identification of intra-laboratory blood culture contamination with Staphylococcus aureus by whole genome sequencing.

Anushia Ashokan, Lito E Papanicolas, Lex E X Leong, Maria Theodossi, Santhosh Daniel, Steve L Wesselingh, Geraint B Rogers, David L Gordon,

Staphylococcus aureus in blood cultures is rarely considered a contaminant. We report a case of intra-laboratory contamination between blood culture bottles which was confirmed by whole genome sequencing, highlighting the importance of molecular analysis in the clinical laboratory setting. ... Read more >>

Diagn Microbiol Infect Dis (Diagnostic microbiology and infectious disease)
[2019, 94(4):331-333]

Cited: 0 times

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Bacterial viability in faecal transplants: Which bacteria survive?

Lito E Papanicolas, Jocelyn M Choo, Yanan Wang, Lex E X Leong, Samuel P Costello, David L Gordon, Steve L Wesselingh, Geraint B Rogers,

<h4>Background</h4>The therapeutic potential of faecal microbiota transplantation (FMT) is under investigation for a range of inflammatory conditions. While mechanisms of benefit are poorly understood, most models rely on the viability of transplanted microbes. We hypothesised that protocols commonly used in the preparation of faecal transplants will substantially reduce the number, ... Read more >>

EBioMedicine (EBioMedicine)
[2019, 41:509-516]

Cited: 22 times

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B Part of It study: a longitudinal study to assess carriage of Neisseria meningitidis in first year university students in South Australia.

Mark McMillan, Luke Walters, Turra Mark, Andrew Lawrence, Lex E X Leong, Thomas Sullivan, Geraint B Rogers, Ross M Andrews, Helen S Marshall,

<h4>Objectives</h4>N. meningitidis carriage in Australia is poorly understood. This study aimed to estimate prevalence and risk factors for carriage of N. meningitidis in South Australian university students. We also sought to identify whether delayed freezing of oropharyngeal samples altered PCR positivity, cycle threshold, or culture positivity.<h4>Methods</h4>Oropharyngeal swabs were taken from ... Read more >>

Hum Vaccin Immunother (Human vaccines & immunotherapeutics)
[2019, 15(4):987-994]

Cited: 6 times

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Opportunistic bacteria confer the ability to ferment prebiotic starch in the adult cystic fibrosis gut.

Yanan Wang, Lex E X Leong, Rebecca L Keating, Tokuwa Kanno, Guy C J Abell, Fredrick M Mobegi, Jocelyn M Choo, Steve L Wesselingh, A James Mason, Lucy D Burr, Geraint B Rogers,

Chronic disruption of the intestinal microbiota in adult cystic fibrosis (CF) patients is associated with local and systemic inflammation, and has been linked to the risk of serious comorbidities. Supplementation with high amylose maize starch (HAMS) might provide clinical benefit by promoting commensal bacteria and the biosynthesis of immunomodulatory metabolites. ... Read more >>

Gut Microbes (Gut microbes)
[2019, 10(3):367-381]

Cited: 9 times

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Burkholderia lata Infections from Intrinsically Contaminated Chlorhexidine Mouthwash, Australia, 2016.

Lex E X Leong, Diana Lagana, Glen P Carter, Qinning Wang, Kija Smith, Tim P Stinear, David Shaw, Vitali Sintchenko, Steven L Wesselingh, Ivan Bastian, Geraint B Rogers,

Burkholderia lata was isolated from 8 intensive care patients at 2 tertiary hospitals in Australia. Whole-genome sequencing demonstrated that clinical and environmental isolates originated from a batch of contaminated commercial chlorhexidine mouthwash. Genomic analysis identified efflux pump-encoding genes as potential facilitators of bacterial persistence within this biocide. ... Read more >>

Emerg Infect Dis (Emerging infectious diseases)
[2018, 24(11):2109-2111]

Cited: 5 times

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Impact of Long-Term Erythromycin Therapy on the Oropharyngeal Microbiome and Resistance Gene Reservoir in Non-Cystic Fibrosis Bronchiectasis.

Jocelyn M Choo, Guy C J Abell, Rachel Thomson, Lucy Morgan, Grant Waterer, David L Gordon, Steven L Taylor, Lex E X Leong, Steve L Wesselingh, Lucy D Burr, Geraint B Rogers,

Long-term macrolide therapy reduces rates of pulmonary exacerbation in bronchiectasis. However, little is known about the potential for macrolide therapy to alter the composition and function of the oropharyngeal commensal microbiota or to increase the carriage of transmissible antimicrobial resistance. We assessed the effect of long-term erythromycin on oropharyngeal microbiota ... Read more >>

mSphere (mSphere)
[2018, 3(2):]

Cited: 13 times

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Understanding the impact of antibiotic therapies on the respiratory tract resistome: a novel pooled-template metagenomic sequencing strategy.

Steven L Taylor, Lex E X Leong, Fredrick M Mobegi, Jocelyn M Choo, Lucy D Burr, Steve Wesselingh, Geraint B Rogers,

Determining the effects of antimicrobial therapies on airway microbiology at a population-level is essential. Such analysis allows, for example, surveillance of antibiotic-induced changes in pathogen prevalence, the emergence and spread of antibiotic resistance, and the transmission of multi-resistant organisms. However, current analytical strategies for understanding these processes are limited. Culture- ... Read more >>

Multidiscip Respir Med (Multidisciplinary respiratory medicine)
[2018, 13(Suppl 1):30]

Cited: 3 times

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Draft Genome Sequence of a Non-O1/O139 Vibrio cholerae Strain Isolated from a Patient Presenting with Dysuria.

Lex E X Leong, David L Gordon, Geraint B Rogers,

Vibrio cholerae is the causative agent of cholera and, more rarely, nondiarrheal opportunistic infections. We report here a draft genome sequence of a non-O1/O139 V. cholerae strain isolated from the urine of a patient presenting with dysuria at a South Australian health care facility. The genome analysis revealed several virulence ... Read more >>

Microbiol Resour Announc (Microbiology resource announcements)
[2018, 7(3):]

Cited: 0 times

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Clinical and symptom scores are significantly correlated with fecal microbiota features in patients with symptomatic uncomplicated diverticular disease: a pilot study.

Charlotte L Kvasnovsky, Lex E X Leong, Jocelyn M Choo, Guy C J Abell, Savvas Papagrigoriadis, Kenneth D Bruce, Geraint B Rogers,

There is growing consensus that symptomatic uncomplicated diverticular disease is a chronic inflammatory condition, and that alterations in the fecal microbiota may contribute to its pathogenesis.The aim of this study was to relate the fecal microbiota composition in symptomatic uncomplicated diverticular disease to measures of inflammation, symptoms, and history of ... Read more >>

Eur J Gastroenterol Hepatol (European journal of gastroenterology & hepatology)
[2018, 30(1):107-112]

Cited: 8 times

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Draft Genome Sequences of Two Enterobacter cloacae subsp. cloacae Strains Isolated from Australian Hematology Patients with Bacteremia.

Lex E X Leong, David Shaw, Lito Papanicolas, Diana Lagana, Ivan Bastian, Geraint B Rogers,

<i>Enterobacter cloacae</i> is a common member of the gut microbiota in healthy individuals. However, it is also an opportunistic pathogen, capable of causing bacteremia. We report the draft genomes of two <i>Enterobacter cloacae</i> subspecies <i>cloacae</i> strains isolated from hematology patients with bacteremia. Both isolates carry genes encoding extended-spectrum β-lactamases. ... Read more >>

Genome Announc (Genome announcements)
[2017, 5(33):]

Cited: 0 times

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Intestinal Microbiota Composition in Sudden Infant Death Syndrome and Age-Matched Controls.

Lex E X Leong, Steven L Taylor, Aravind Shivasami, Paul N Goldwater, Geraint B Rogers,

<h4>Objective</h4>To assess whether features of the infant intestinal microbiome, including the carriage of toxigenic bacteria, are associated with sudden infant death syndrome (SIDS).<h4>Study design</h4>We undertook a case-controlled analysis of fecal microbiology in SIDS. Fecal material was obtained from 44 cases and 44 aged-matched controls. Microbiota composition was determined by 16S ... Read more >>

J Pediatr (The Journal of pediatrics)
[2017, 191:63-68.e1]

Cited: 3 times

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Effect of azithromycin on asthma exacerbations and quality of life in adults with persistent uncontrolled asthma (AMAZES): a randomised, double-blind, placebo-controlled trial.

Peter G Gibson, Ian A Yang, John W Upham, Paul N Reynolds, Sandra Hodge, Alan L James, Christine Jenkins, Matthew J Peters, Guy B Marks, Melissa Baraket, Heather Powell, Steven L Taylor, Lex E X Leong, Geraint B Rogers, Jodie L Simpson,

<h4>Background</h4>Exacerbations of asthma cause a substantial global illness burden. Adults with uncontrolled persistent asthma despite maintenance treatment require additional therapy. Since macrolide antibiotics can be used to treat persistent asthma, we aimed to assess the efficacy and safety of oral azithromycin as add-on therapy in patients with uncontrolled persistent asthma ... Read more >>

Lancet (Lancet (London, England))
[2017, 390(10095):659-668]

Cited: 132 times

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Inflammatory phenotypes in patients with severe asthma are associated with distinct airway microbiology.

Steven L Taylor, Lex E X Leong, Jocelyn M Choo, Steve Wesselingh, Ian A Yang, John W Upham, Paul N Reynolds, Sandra Hodge, Alan L James, Christine Jenkins, Matthew J Peters, Melissa Baraket, Guy B Marks, Peter G Gibson, Jodie L Simpson, Geraint B Rogers,

<h4>Background</h4>Asthma pathophysiology and treatment responsiveness are predicted by inflammatory phenotype. However, the relationship between airway microbiology and asthma phenotype is poorly understood.<h4>Objective</h4>We aimed to characterize the airway microbiota in patients with symptomatic stable asthma and relate composition to airway inflammatory phenotype and other phenotypic characteristics.<h4>Methods</h4>The microbial composition of induced sputum ... Read more >>

J Allergy Clin Immunol (The Journal of allergy and clinical immunology)
[2018, 141(1):94-103.e15]

Cited: 64 times

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Divergent Relationships between Fecal Microbiota and Metabolome following Distinct Antibiotic-Induced Disruptions.

Jocelyn M Choo, Tokuwa Kanno, Nur Masirah Mohd Zain, Lex E X Leong, Guy C J Abell, Julie E Keeble, Kenneth D Bruce, A James Mason, Geraint B Rogers,

The intestinal microbiome plays an essential role in regulating many aspects of host physiology, and its disruption through antibiotic exposure has been implicated in the development of a range of serious pathologies. The complex metabolic relationships that exist between members of the intestinal microbiota and the potential redundancy in functional ... Read more >>

mSphere (mSphere)
[2017, 2(1):]

Cited: 12 times

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Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples.

Gianny P Scoleri, Jocelyn M Choo, Lex E X Leong, Thomas R Goddard, Lisa Shephard, Lucy D Burr, Ivan Bastian, Rachel M Thomson, Geraint B Rogers,

Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust. ... Read more >>

J Clin Microbiol (Journal of clinical microbiology)
[2016, 54(9):2395-2398]

Cited: 1 time

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