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An investigation of Burkholderia cepacia complex methylomes via SMRT sequencing and mutant analysis

Olga Mannweiler, Marta Pinto-Carbó, Martina Lardi, Kirsty Agnoli, Leo Eberl,

Abstract The Burkholderia cepacia complex (Bcc) is a group of 22 closely related opportunistic pathogens which produce a wide range of bioactive secondary metabolites with great biotechnological potential, for example in biocontrol and bioremediation. This study aimed to investigate methylation in the Bcc by SMRT sequencing, and to determine the ... Read more >>

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Copper resistance genes of Burkholderia cenocepacia H111 identified by transposon sequencing.

Steven Higgins, Stefano Gualdi, Marta Pinto-Carbó, Leo Eberl,

Copper is an essential element but in excess is highly toxic and therefore cytoplasmic levels must be tightly controlled. Member of the genus Burkholderia are highly resistant to various heavy metals and are often isolated from acidic soils where copper bioavailability is high. In this study, we employed transposon sequencing ... Read more >>

Environ Microbiol Rep (Environmental microbiology reports)
[2020, 12(2):241-249]

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DNA methylation epigenetically regulates gene expression in Burkholderia cenocepacia

Ian Vandenbussche, Andrea Sass, Marta Pinto-Carbó, Olga Mannweiler, Leo Eberl, Tom Coenye,

Abstract Respiratory tract infections by the opportunistic pathogen Burkholderia cenocepacia often lead to severe lung damage in cystic fibrosis (CF) patients. New insights in how to tackle these infections might emerge from the field of epigenetics, as DNA methylation has shown to be an important regulator of gene expression. The ... Read more >>

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Biosynthesis and Structure-Activity Relationship Investigations of the Diazeniumdiolate Antifungal Agent Fragin.

Simon Sieber, Christophe Daeppen, Christian Jenul, Vidya Mannancherril, Leo Eberl, Karl Gademann,

Only a few natural products incorporating a diazeniumdiolate moiety have been isolated, and these compounds usually display a broad range of biological activities. Only recently has the first diazeniumdiolate natural product biosynthetic gene cluster been identified in Burkholderia cenocepacia H111, which produces the fungicide (-)-fragin and the signal molecule rac-valdiazen. ... Read more >>

Chembiochem (Chembiochem : a European journal of chemical biology)
[2020, 21(11):1587-1592]

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Author Correction: Chimeric peptidomimetic antibiotics against Gram-negative bacteria.

Anatol Luther, Matthias Urfer, Michael Zahn, Maik Müller, Shuang-Yan Wang, Milon Mondal, Alessandra Vitale, Jean-Baptiste Hartmann, Timothy Sharpe, Fabio Lo Monte, Harsha Kocherla, Elizabeth Cline, Gabriella Pessi, Parthasarathi Rath, Seyed Majed Modaresi, Petra Chiquet, Sarah Stiegeler, Carolin Verbree, Tobias Remus, Michel Schmitt, Caroline Kolopp, Marie-Anne Westwood, Nicolas Desjonquères, Emile Brabet, Sophie Hell, Karen LePoupon, Annie Vermeulen, Régis Jaisson, Virginie Rithié, Grégory Upert, Alexander Lederer, Peter Zbinden, Achim Wach, Kerstin Moehle, Katja Zerbe, Hans H Locher, Francesca Bernardini, Glenn E Dale, Leo Eberl, Bernd Wollscheid, Sebastian Hiller, John A Robinson, Daniel Obrecht,

An Amendment to this paper has been published and can be accessed via a link at the top of the paper. ... Read more >>

Nature (Nature)
[2019, 576(7786):E5]

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The effect of flow on swimming bacteria controls the initial colonization of curved surfaces

Eleonora Secchi, Alessandra Vitale, Gastón Miño, Vasily Kantsler, Leo Eberl, Roberto Rusconi, Roman Stocker,

Abstract The colonization of solid surfaces by bacteria is a widespread phenomenon with major consequences on environmental processes, biotechnology and human health. While much is known about the molecular mechanisms of surface colonization, the influence of the physical environment remains poorly understood. Here we show that the magnitude and location ... Read more >>

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Chimeric peptidomimetic antibiotics against Gram-negative bacteria.

Anatol Luther, Matthias Urfer, Michael Zahn, Maik Müller, Shuang-Yan Wang, Milon Mondal, Alessandra Vitale, Jean-Baptiste Hartmann, Timothy Sharpe, Fabio Lo Monte, Harsha Kocherla, Elizabeth Cline, Gabriella Pessi, Parthasarathi Rath, Seyed Majed Modaresi, Petra Chiquet, Sarah Stiegeler, Carolin Verbree, Tobias Remus, Michel Schmitt, Caroline Kolopp, Marie-Anne Westwood, Nicolas Desjonquères, Emile Brabet, Sophie Hell, Karen LePoupon, Annie Vermeulen, Régis Jaisson, Virginie Rithié, Grégory Upert, Alexander Lederer, Peter Zbinden, Achim Wach, Kerstin Moehle, Katja Zerbe, Hans H Locher, Francesca Bernardini, Glenn E Dale, Leo Eberl, Bernd Wollscheid, Sebastian Hiller, John A Robinson, Daniel Obrecht,

There is an urgent need for new antibiotics against Gram-negative pathogens that are resistant to carbapenem and third-generation cephalosporins, against which antibiotics of last resort have lost most of their efficacy. Here we describe a class of synthetic antibiotics inspired by scaffolds derived from natural products. These chimeric antibiotics contain ... Read more >>

Nature (Nature)
[2019, 576(7787):452-458]

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Genetic architecture constrains exploitation of siderophore cooperation in the bacterium Burkholderia cenocepacia.

Santosh Sathe, Anugraha Mathew, Kirsty Agnoli, Leo Eberl, Rolf Kümmerli,

Explaining how cooperation can persist in the presence of cheaters, exploiting the cooperative acts, is a challenge for evolutionary biology. Microbial systems have proved extremely useful to test evolutionary theory and identify mechanisms maintaining cooperation. One of the most widely studied system is the secretion and sharing of iron-scavenging siderophores ... Read more >>

Evol Lett (Evolution letters)
[2019, 3(6):610-622]

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An Integrated Systems Approach Unveils New Aspects of Microoxia-Mediated Regulation in Bradyrhizobium diazoefficiens.

Noemí Fernández, Juan J Cabrera, Adithi R Varadarajan, Stefanie Lutz, Raphael Ledermann, Bernd Roschitzki, Leo Eberl, Eulogio J Bedmar, Hans-Martin Fischer, Gabriella Pessi, Christian H Ahrens, Socorro Mesa,

The adaptation of rhizobia from the free-living state in soil to the endosymbiotic state comprises several physiological changes in order to cope with the extremely low oxygen availability (microoxia) within nodules. To uncover cellular functions required for bacterial adaptation to microoxia directly at the protein level, we applied a systems ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2019, 10:924]

Cited: 1 time

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Genetic architecture constrains exploitation of siderophore cooperation inBurkholderia cenocepacia

Santosh Sathe, Anugraha Mathew, Kirsty Agnoli, Leo Eberl, Rolf Kümmerli,

Explaining how cooperation can persist in the presence of cheaters, exploiting the cooperative acts, is a challenge for evolutionary biology. While microbial systems have proved extremely useful to test evolutionary theory and identify mechanisms maintaining cooperation, our knowledge is often limited to insights gained from a few model organisms. Here, ... Read more >>

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The Compound 2-Hexyl, 5-Propyl Resorcinol Has a Key Role in Biofilm Formation by the Biocontrol Rhizobacterium Pseudomonas chlororaphis PCL1606.

Claudia E Calderón, Sandra Tienda, Zaira Heredia-Ponce, Eva Arrebola, Gerardo Cárcamo-Oyarce, Leo Eberl, Francisco M Cazorla,

The production of the compound 2-hexyl-5-propyl resorcinol (HPR) by the biocontrol rhizobacterium Pseudomonas chlororaphis PCL1606 (PcPCL1606) is crucial for fungal antagonism and biocontrol activity that protects plants against the phytopathogenic fungus Rosellinia necatrix. The production of HPR is also involved in avocado root colonization during the biocontrol process. This pleiotrophic ... Read more >>

Front Microbiol (Frontiers in Microbiology)
[2019, 10:396]

Cited: 1 time

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Bacterial Adhesion on Soft Materials: Passive Physicochemical Interactions or Active Bacterial Mechanosensing?

Hervé Straub, Claudio M Bigger, Jules Valentin, Dominik Abt, Xiao-Hua Qin, Leo Eberl, Katharina Maniura-Weber, Qun Ren,

The influence of mechanical stiffness of biomaterials on bacterial adhesion is only sparsely studied and the mechanism behind this influence remains unclear. Here, bacterial adhesion on polydimethylsiloxane (PDMS) samples, having four different degrees of stiffness with Young's modulus ranging from 0.06 to 4.52 MPa, is investigated. Escherichia coli and Pseudomonas ... Read more >>

Adv Healthc Mater (Advanced healthcare materials)
[2019, 8(8):e1801323]

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Burkholderia cenocepacia utilizes a type VI secretion system for bacterial competition.

Helena L Spiewak, Sravanthi Shastri, Lili Zhang, Stephan Schwager, Leo Eberl, Annette C Vergunst, Mark S Thomas,

Burkholderia cenocepacia is an opportunistic bacterial pathogen that poses a significant threat to individuals with cystic fibrosis by provoking a strong inflammatory response within the lung. It possesses a type VI secretion system (T6SS), a secretory apparatus that can perforate the cellular membrane of other bacterial species and/or eukaryotic targets, ... Read more >>

Microbiologyopen (MicrobiologyOpen)
[2019, :e774]

Cited: 3 times

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The afc antifungal activity cluster, which is under tight regulatory control of ShvR, is essential for transition from intracellular persistence of Burkholderia cenocepacia to acute pro-inflammatory infection.

Margarida C Gomes, Yara Tasrini, Sujatha Subramoni, Kirsty Agnoli, Joana R Feliciano, Leo Eberl, Pamela Sokol, David O'Callaghan, Annette C Vergunst,

The opportunistic pathogen Burkholderia cenocepacia is particularly life-threatening for cystic fibrosis (CF) patients. Chronic lung infections with these bacteria can rapidly develop into fatal pulmonary necrosis and septicaemia. We have recently shown that macrophages are a critical site for replication of B. cenocepacia K56-2 and the induction of fatal pro-inflammatory ... Read more >>

PLoS Pathog. (PLoS pathogens)
[2018, 14(12):e1007473]

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Synthesis and Biological Evaluation of the Novel Growth Inhibitor Streptol Glucoside, Isolated from an Obligate Plant Symbiont.

Chien-Chi Hsiao, Simon Sieber, Antri Georgiou, Aurélien Bailly, Despina Emmanouilidou, Aurélien Carlier, Leo Eberl, Karl Gademann,

The plant Psychotria kirkii hosts an obligatory bacterial symbiont, Candidatus Burkholderia kirkii, in nodules on their leaves. Recently, a glucosylated derivative of (+)-streptol, (+)-streptol glucoside, was isolated from the nodulated leaves and was found to possess a plant growth inhibitory activity. To establish a structure-activity relationship study, a convergent strategy ... Read more >>

Chemistry (Chemistry (Weinheim an der Bergstrasse, Germany))
[2019, 25(7):1722-1726]

Cited: 1 time

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Types and origins of bacterial membrane vesicles.

Masanori Toyofuku, Nobuhiko Nomura, Leo Eberl,

Most bacteria release membrane vesicles (MVs) that contain specific cargo molecules and have diverse functions, including the transport of virulence factors, DNA transfer, interception of bacteriophages, antibiotics and eukaryotic host defence factors, cell detoxification and bacterial communication. MVs not only are abundant in nature but also show great promise for ... Read more >>

Nat. Rev. Microbiol. (Nature reviews. Microbiology)
[2019, 17(1):13-24]

Cited: 12 times

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Involvement of Burkholderiaceae and sulfurous volatiles in disease-suppressive soils.

Víctor J Carrión, Viviane Cordovez, Olaf Tyc, Desalegn W Etalo, Irene de Bruijn, Victor C L de Jager, Marnix H Medema, Leo Eberl, Jos M Raaijmakers,

Disease-suppressive soils are ecosystems in which plants suffer less from root infections due to the activities of specific microbial consortia. The characteristics of soils suppressive to specific fungal root pathogens are comparable to those of adaptive immunity in animals, as reported by Raaijmakers and Mazzola (Science 352:1392-3, 2016), but the ... Read more >>

ISME J (The ISME journal)
[2018, 12(9):2307-2321]

Cited: 8 times

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Biosynthesis of fragin is controlled by a novel quorum sensing signal.

Christian Jenul, Simon Sieber, Christophe Daeppen, Anugraha Mathew, Martina Lardi, Gabriella Pessi, Dominic Hoepfner, Markus Neuburger, Anthony Linden, Karl Gademann, Leo Eberl,

Members of the diazeniumdiolate class of natural compounds show potential for drug development because of their antifungal, antibacterial, antiviral, and antitumor activities. Yet, their biosynthesis has remained elusive to date. Here, we identify a gene cluster directing the biosynthesis of the diazeniumdiolate compound fragin in Burkholderia cenocepacia H111. We provide ... Read more >>

Nat Commun (Nature communications)
[2018, 9(1):1297]

Cited: 8 times

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Leaf nodule symbiosis: function and transmission of obligate bacterial endophytes.

Marta Pinto-Carbó, Karl Gademann, Leo Eberl, Aurelien Carlier,

Various plant species establish intimate symbioses with bacteria within their aerial organs. The bacteria are contained within nodules or glands often present in distinctive patterns on the leaves, and have been used as taxonomic marker since the early 20th century. These structures are present in very diverse taxa, including dicots ... Read more >>

Curr. Opin. Plant Biol. (Current opinion in plant biology)
[2018, 44:23-31]

Cited: 4 times

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Identification of AHL- and BDSF-Controlled Proteins in Burkholderia cenocepacia by Proteomics.

Yilei Liu, Gabriella Pessi, Katharina Riedel, Leo Eberl,

We used comparative proteome analysis to determine the target genes of the two quorum sensing (QS) circuits in the opportunistic pathogen Burkholderia cenocepacia: the N-acyl homoserine lactone (AHL)-based CepIR system and the BDSF (B urkholderia diffusible signal factor, cis-2-dodecenoic acid)-based RpfFR system. In this book chapter, we focus on the ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2018, 1673:193-202]

Cited: 1 time

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Mutations in Two Paraburkholderia phymatum Type VI Secretion Systems Cause Reduced Fitness in Interbacterial Competition.

Samanta Bolzan de Campos, Martina Lardi, Alessia Gandolfi, Leo Eberl, Gabriella Pessi,

Paraburkholderia phymatum is a highly effective microsymbiont of Mimosa spp. and has also been shown to nodulate papilionoid legumes. P. phymatum was found to be highly competitive both in a natural environment as well as under controlled test conditions and is more competitive for nodulation over other α- and β-rhizobial ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2017, 8:2473]

Cited: 1 time

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Heterologous Expression, Biosynthetic Studies, and Ecological Function of the Selective Gq-Signaling Inhibitor FR900359.

Max Crüsemann, Raphael Reher, Isabella Schamari, Alexander O Brachmann, Tsubasa Ohbayashi, Markus Kuschak, Davide Malfacini, Alexander Seidinger, Marta Pinto-Carbó, René Richarz, Tatjana Reuter, Stefan Kehraus, Asis Hallab, Misty Attwood, Helgi B Schiöth, Peter Mergaert, Yoshitomo Kikuchi, Till F Schäberle, Evi Kostenis, Daniela Wenzel, Christa E Müller, Jörn Piel, Aurélien Carlier, Leo Eberl, Gabriele M König,

The cyclic depsipeptide FR900359 (FR), isolated from the tropical plant Ardisia crenata, is a strong and selective inhibitor of Gq proteins, making it an indispensable pharmacological tool to study Gq-related processes, as well as a promising drug candidate. Gq inhibition is a novel mode of action for defense chemicals and ... Read more >>

Angew. Chem. Int. Ed. Engl. (Angewandte Chemie (International ed. in English))
[2018, 57(3):836-840]

Cited: 5 times

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Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia cenocepacia.

Anja M Richter, Mustafa Fazli, Nadine Schmid, Rebecca Shilling, Angela Suppiger, Michael Givskov, Leo Eberl, Tim Tolker-Nielsen,

Burkholderia cenocepacia H111 is an opportunistic pathogen associated with chronic lung infections in cystic fibrosis patients. Biofilm formation, motility and virulence of B. cenocepacia are regulated by the second messenger cyclic di-guanosine monophosphate (c-di-GMP). In the present study, we analyzed the role of all 25 putative c-di-GMP metabolizing proteins of ... Read more >>

Front Microbiol (Frontiers in microbiology)
[2018, 9:3286]

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The Essential Genome of Burkholderia cenocepacia H111.

Steven Higgins, Maria Sanchez-Contreras, Stefano Gualdi, Marta Pinto-Carbó, Aurélien Carlier, Leo Eberl,

The study of the minimum set of genes required to sustain life is a fundamental question in biological research. Recent studies on bacterial essential genes suggested that between 350 and 700 genes are essential to support autonomous bacterial cell growth. Essential genes are of interest as potential new antimicrobial drug ... Read more >>

J. Bacteriol. (Journal of bacteriology)
[2017, 199(22):]

Cited: 2 times

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Draft Genome Sequence of Cronobacter sakazakii GP1999, Sequence Type 145, an Epiphytic Isolate Obtained from the Tomato's Rhizoplane/Rhizosphere Continuum.

Hannah R Chase, Leo Eberl, Roger Stephan, HyeJin Jeong, Chaeyoon Lee, Samantha Finkelstein, Flavia Negrete, Jayanthi Gangiredla, Isha Patel, Ben D Tall, Gopal R Gopinath, Angelika Lehner,

We present here the draft genome of Cronobacter sakazakii GP1999, a sequence type 145 strain isolated from the rhizosphere of tomato plants. Assembly and annotation of the genome resulted in a genome of 4,504,670 bp in size, with 4,148 coding sequences, and a GC content of 56.8%. ... Read more >>

Genome Announc (Genome announcements)
[2017, 5(31):]

Cited: 2 times

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