Full Text Journal Articles by
Author Laura Popolo


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Poacic acid, a β-1,3-glucan-binding antifungal agent, inhibits cell-wall remodeling and activates transcriptional responses regulated by the cell-wall integrity and high-osmolarity glycerol pathways in yeast.

Raúl García, Kaori Itto-Nakama, José Manuel Rodríguez-Peña, Xiaolin Chen, Ana Belén Sanz, Alba de Lorenzo, Mónica Pavón-Vergés, Karen Kubo, Shinsuke Ohnuki, César Nombela, Laura Popolo, Yoshikazu Ohya, Javier Arroyo,

As a result of the relatively few available antifungals and the increasing frequency of resistance to them, the development of novel antifungals is increasingly important. The plant natural product poacic acid (PA) inhibits β-1,3-glucan synthesis in Saccharomyces cerevisiae and has antifungal activity against a wide range of plant pathogens. However, ... Read more >>

FASEB J (FASEB journal : official publication of the Federation of American Societies for Experimental Biology)
[2021, 35(9):e21778]

Cited: 0 times

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Soluble Receptor for Advanced Glycation End-products regulates age-associated Cardiac Fibrosis.

Francesco Scavello, Filippo Zeni, Giuseppina Milano, Federica Macrì, Stefania Castiglione, Estella Zuccolo, Alessandro Scopece, Giovanni Pezone, Calogero C Tedesco, Patrizia Nigro, Genny Degani, Elisa Gambini, Fabrizio Veglia, Laura Popolo, Giulio Pompilio, Gualtiero I Colombo, Marco E Bianchi, Angela Raucci,

Myocardial aging increases the cardiovascular risk in the elderly. The Receptor for Advanced Glycation End-products (RAGE) is involved in age-related disorders. The soluble isoform (sRAGE) acts as a scavenger blocking the membrane-bound receptor activation. This study aims at investigating RAGE contribution to age-related cardiac remodeling. We analyzed the cardiac function ... Read more >>

Int J Biol Sci (International journal of biological sciences)
[2021, 17(10):2399-2416]

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Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity of the Novel Rid (Enamine/Imine Deaminase) Class of Enzymes.

Stefania Digiovanni, Genny Degani, Laura Popolo, Maria Antonietta Vanoni,

This chapter describes a method to assay the activity of reactive intermediate deaminases (Rid), a large family of conserved soluble enzymes, which have been proposed to prevent damages from metabolic intermediates such as the highly reactive and unstable compounds enamines/imines. In this method, the flavin adenine dinucleotide-dependent L- or D-amino ... Read more >>

Methods Mol Biol (Methods in molecular biology (Clifton, N.J.))
[2021, 2280:199-218]

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Two novel fish paralogs provide insights into the Rid family of imine deaminases active in pre-empting enamine/imine metabolic damage.

Stefania Digiovanni, Cristina Visentin, Genny Degani, Alberto Barbiroli, Matteo Chiara, Luca Regazzoni, Flavio Di Pisa, Andrew J Borchert, Diana M Downs, Stefano Ricagno, Maria Antonietta Vanoni, Laura Popolo,

Reactive Intermediate Deaminase (Rid) protein superfamily includes eight families among which the RidA is conserved in all domains of life. RidA proteins accelerate the deamination of the reactive 2-aminoacrylate (2AA), an enamine produced by some pyridoxal phosphate (PLP)-dependent enzymes. 2AA accumulation inhibits target enzymes with a detrimental impact on fitness. ... Read more >>

Sci Rep (Scientific reports)
[2020, 10(1):10135]

Cited: 1 time

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Unveiling the molecular mechanisms underpinning biorecognition of early-glycated human serum albumin and receptor for advanced glycation end products.

Anna Tramarin, Marina Naldi, Genny Degani, Loredana Lupu, Pascal Wiegand, Angelica Mazzolari, Alessandra Altomare, Giancarlo Aldini, Laura Popolo, Giulio Vistoli, Michael Przybylski, Manuela Bartolini,

Serum levels of early-glycated albumin are significantly increased in patients with diabetes mellitus and may play a role in worsening inflammatory status and sustaining diabetes-related complications. To investigate possible pathological recognition involving early-glycated albumin and the receptor for advanced glycation end products (RAGE), an early-glycated human serum albumin (HSAgly), with ... Read more >>

Anal Bioanal Chem (Analytical and bioanalytical chemistry)
[2020, 412(18):4245-4259]

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The Glucan-Remodeling Enzyme Phr1p and the Chitin Synthase Chs1p Cooperate to Maintain Proper Nuclear Segregation and Cell Integrity in Candida albicans.

Genny Degani, Laura Popolo,

GH72 family of β-(1,3)-glucanosyltransferases is unique to fungi and is required for cell wall biogenesis, morphogenesis, virulence, and in some species is essential for life. Candida albicans PHR1 and PHR2 are pH-regulated genes that encode GH72 enzymes highly similar to Gas1p of Saccharomyces cerevisiae. PHR1 is expressed at pH ≥ ... Read more >>

Front Cell Infect Microbiol (Frontiers in cellular and infection microbiology)
[2019, 9:400]

Cited: 0 times

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Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris.

Genny Degani, Alberto Barbiroli, Paula Magnelli, Stefania Digiovanni, Alessandra Altomare, Giancarlo Aldini, Laura Popolo,

Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health. They directly act on proteins, affecting structure and function, or through receptor-mediated mechanisms. RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs. RAGE ... Read more >>

Glycoconj J (Glycoconjugate journal)
[2019, 36(1):27-38]

Cited: 1 time

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Imine Deaminase Activity and Conformational Stability of UK114, the Mammalian Member of the Rid Protein Family Active in Amino Acid Metabolism.

Genny Degani, Alberto Barbiroli, Luca Regazzoni, Laura Popolo, Maria Antonietta Vanoni,

Reactive intermediate deaminase (Rid) protein family is a recently discovered group of enzymes that is conserved in all domains of life and is proposed to play a role in the detoxification of reactive enamines/imines. UK114, the mammalian member of RidA subfamily, was identified in the early 90s as a component ... Read more >>

Int J Mol Sci (International journal of molecular sciences)
[2018, 19(4):]

Cited: 6 times

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The PHR Family: The Role of Extracellular Transglycosylases in Shaping Candida albicans Cells.

Laura Popolo, Genny Degani, Carlo Camilloni, William A Fonzi,

Candida albicans is an opportunistic microorganism that can become a pathogen causing mild superficial mycosis or more severe invasive infections that can be life-threatening for debilitated patients. In the etiology of invasive infections, key factors are the adaptability of C. albicans to the different niches of the human body and ... Read more >>

J Fungi (Basel) (Journal of fungi (Basel, Switzerland))
[2017, 3(4):]

Cited: 6 times

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The Dual Activity Responsible for the Elongation and Branching of β-(1,3)-Glucan in the Fungal Cell Wall.

Vishukumar Aimanianda, Catherine Simenel, Cecile Garnaud, Cecile Clavaud, Rui Tada, Lise Barbin, Isabelle Mouyna, Christoph Heddergott, Laura Popolo, Yoshikazu Ohya, Muriel Delepierre, Jean-Paul Latge,

β-(1,3)-Glucan, the major fungal cell wall component, ramifies through β-(1,6)-glycosidic linkages, which facilitates its binding with other cell wall components contributing to proper cell wall assembly. Using <i>Saccharomyces cerevisiae</i> as a model, we developed a protocol to quantify β-(1,6)-branching on β-(1,3)-glucan. Permeabilized <i>S. cerevisiae</i> and radiolabeled substrate UDP-(<sup>14</sup>C)glucose allowed us to ... Read more >>

mBio (mBio)
[2017, 8(3):]

Cited: 24 times

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A capture method based on the VC1 domain reveals new binding properties of the human receptor for advanced glycation end products (RAGE).

Genny Degani, Alessandra A Altomare, Mara Colzani, Caterina Martino, Angelica Mazzolari, Guenter Fritz, Giulio Vistoli, Laura Popolo, Giancarlo Aldini,

The Advanced Glycation and Lipoxidation End products (AGEs and ALEs) are a heterogeneous class of compounds derived from the non-enzymatic glycation or protein adduction by lipoxidation break-down products. The receptor for AGEs (RAGE) is involved in the progression of chronic diseases based on persistent inflammatory state and oxidative stress. RAGE ... Read more >>

Redox Biol (Redox biology)
[2017, 11:275-285]

Cited: 6 times

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Neutrophil Attack Triggers Extracellular Trap-Dependent Candida Cell Wall Remodeling and Altered Immune Recognition.

Alex Hopke, Nadine Nicke, Erica E Hidu, Genny Degani, Laura Popolo, Robert T Wheeler,

Pathogens hide immunogenic epitopes from the host to evade immunity, persist and cause infection. The opportunistic human fungal pathogen Candida albicans, which can cause fatal disease in immunocompromised patient populations, offers a good example as it masks the inflammatory epitope β-glucan in its cell wall from host recognition. It has ... Read more >>

PLoS Pathog (PLoS pathogens)
[2016, 12(5):e1005644]

Cited: 55 times

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An improved expression system for the VC1 ligand binding domain of the receptor for advanced glycation end products in Pichia pastoris.

Genny Degani, Mara Colzani, Alberto Tettamanzi, Luca Sorrentino, Alessandro Aliverti, Guenter Fritz, Giancarlo Aldini, Laura Popolo,

The receptor for the advanced glycation end products (RAGE) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily and binds a variety of unrelated ligands sharing a negative charge. Most ligands bind to the extracellular V or VC1 domains of the receptor. In this work, V and VC1 ... Read more >>

Protein Expr Purif (Protein expression and purification)
[2015, 114:48-57]

Cited: 5 times

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Catalytic properties of Phr family members of cell wall glucan remodeling enzymes: implications for the adaptation of Candida albicans to ambient pH.

Kristína Kováčová, Genny Degani, Eva Stratilová, Vladimír Farkaš, Laura Popolo,

Fungal wall formation is a dynamic process involving several categories of enzymes. The GH72 family of β(1,3)-glucanosyltransferases is essential for the determination of cell shape, for cell integrity and for virulence in pathogenic fungi. Candida albicans has five GH72 genes: PHR1 and PHR2 are pH dependent, the first being expressed ... Read more >>

FEMS Yeast Res (FEMS yeast research)
[2015, 15(2):]

Cited: 7 times

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Expression and phylogenetic analyses of the Gel/Gas proteins of Tuber melanosporum provide insights into the function and evolution of glucan remodeling enzymes in fungi.

Fabiano Sillo, Carmela Gissi, Daniele Chignoli, Enrico Ragni, Laura Popolo, Raffaella Balestrini,

The β(1,3)-glucanosyltransferases of the GH72 family are redundant enzymes that are essential for the formation and dynamic remodeling of the fungal wall during different stages of the life cycle. Four putative genes encoding glycosylphosphatidylinositol (GPI)-anchored β(1,3)-glucanosyltransferases, designated TmelGEL1, TmelGEL2, TmelGEL4 and TmelGAS4, have been annotated in the genome of Tuber ... Read more >>

Fungal Genet Biol (Fungal genetics and biology : FG & B)
[2013, 53:10-21]

Cited: 10 times

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Catalytic properties of the Gas family β-(1,3)-glucanosyltransferases active in fungal cell-wall biogenesis as determined by a novel fluorescent assay.

Marián Mazáň, Enrico Ragni, Laura Popolo, Vladimír Farkaš,

BGTs [β-(1,3)-glucanosyltransglycosylases; EC 2.4.1.-] of the GH72 (family 72 of glycosylhydrolases) are GPI (glycosylphosphatidylinositol)-anchored proteins that play an important role in the biogenesis of fungal cell walls. They randomly cleave glycosidic linkages in β-(1,3)-glucan chains and ligate the polysaccharide portions containing newly formed reducing ends to C(3)(OH) at non-reducing ends ... Read more >>

Biochem J (The Biochemical journal)
[2011, 438(2):275-282]

Cited: 15 times

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Expression, stability, and replacement of glucan-remodeling enzymes during developmental transitions in Saccharomyces cerevisiae.

Eleonora Rolli, Enrico Ragni, María de Medina-Redondo, Javier Arroyo, Carlos R Vázquez de Aldana, Laura Popolo,

Sporulation is a developmental variation of the yeast life cycle whereby four spores are produced within a diploid cell, with proliferation resuming after germination. The GAS family of glycosylphosphatidylinositol-anchored glucan-remodeling enzymes exemplifies functional interplay between paralogous genes during the yeast life cycle. GAS1 and GAS5 are expressed in vegetative cells ... Read more >>

Mol Biol Cell (Molecular biology of the cell)
[2011, 22(9):1585-1598]

Cited: 19 times

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Phr1p, a glycosylphosphatidylinsitol-anchored β(1,3)-glucanosyltransferase critical for hyphal wall formation, localizes to the apical growth sites and septa in Candida albicans.

Enrico Ragni, Julia Calderon, Umberto Fascio, Matthias Sipiczki, William A Fonzi, Laura Popolo,

Cell wall biogenesis is a dynamic process relying on the coordinated activity of several extracellular enzymes. PHR1 is a pH-regulated gene of Candida albicans encoding a glycosylphosphatidylinositol-anchored β(1,3)-glucanosyltransferase of family GH72 which acts as a cell wall remodelling enzyme and is crucial for morphogenesis and virulence. In order to explore ... Read more >>

Fungal Genet Biol (Fungal genetics and biology : FG & B)
[2011, 48(8):793-805]

Cited: 19 times

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The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections.

Enrico Ragni, Heidi Piberger, Christine Neupert, Jesús García-Cantalejo, Laura Popolo, Javier Arroyo, Markus Aebi, Sabine Strahl,

<h4>Background</h4>Mannoproteins construct the outer cover of the fungal cell wall. The covalently linked cell wall protein Ccw12p is an abundant mannoprotein. It is considered as crucial structural cell wall component since in baker's yeast the lack of CCW12 results in severe cell wall damage and reduced mating efficiency.<h4>Results</h4>In order to ... Read more >>

BMC Genomics (BMC genomics)
[2011, 12:107]

Cited: 24 times

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Generation of an evolved Saccharomyces cerevisiae strain with a high freeze tolerance and an improved ability to grow on glycerol.

Annamaria Merico, Enrico Ragni, Silvia Galafassi, Laura Popolo, Concetta Compagno,

Glycerol is a residue generated during biodiesel production and represents around 10% of the total product output. Biodiesel production is currently having a significant impact on glycerol price, leading to an increased interest in the use of glycerol as a cheap substrate for fermentation processes. We have analysed the growth ... Read more >>

J Ind Microbiol Biotechnol (Journal of industrial microbiology & biotechnology)
[2011, 38(8):1037-1044]

Cited: 10 times

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GAS3, a developmentally regulated gene, encodes a highly mannosylated and inactive protein of the Gas family of Saccharomyces cerevisiae.

Eleonora Rolli, Enrico Ragni, José Manuel Rodriguez-Peña, Javier Arroyo, Laura Popolo,

The multigene GAS family of Saccharomyces cerevisiae is constituted by five genes encoding GPI-anchored proteins required for cell wall or spore wall assembly. GAS1 and GAS5 are expressed in vegetative growth and repressed during sporulation, whereas GAS2 and GAS4 exhibit the opposite expression pattern. This study focuses on GAS3, a ... Read more >>

Yeast (Yeast (Chichester, England))
[2010, 27(8):597-610]

Cited: 12 times

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Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast.

Eleonora Rolli, Enrico Ragni, Julia Calderon, Silvia Porello, Umberto Fascio, Laura Popolo,

Gas1p is a glucan-elongase that plays a crucial role in yeast morphogenesis. It is predominantly anchored to the plasma membrane through a glycosylphosphatidylinositol, but a fraction was also found covalently bound to the cell wall. We have used fusions with the green fluorescent protein or red fluorescent protein (RFP) to ... Read more >>

Mol Biol Cell (Molecular biology of the cell)
[2009, 20(22):4856-4870]

Cited: 31 times

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PHR1, a pH-regulated gene of Candida albicans encoding a glucan-remodelling enzyme, is required for adhesion and invasion.

Julia Calderon, Martin Zavrel, Enrico Ragni, William A Fonzi, Steffen Rupp, Laura Popolo,

The fungal cell wall plays a crucial role in host-pathogen interactions. Its formation is the result of the coordinated activity of several extracellular enzymes, which assemble the constituents, and remodel and hydrolyse them in the extracellular space. Candida albicans Phr1 and Phr2 proteins belong to family GH72 of the beta-(1,3)-glucanosyltransferases ... Read more >>

Microbiology (Reading) (Microbiology (Reading, England))
[2010, 156(Pt 8):2484-2494]

Cited: 39 times

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Disulfide bond structure and domain organization of yeast beta(1,3)-glucanosyltransferases involved in cell wall biogenesis.

Laura Popolo, Enrico Ragni, Cristina Carotti, Oscar Palomares, Ronald Aardema, Jaap Willem Back, Henk L Dekker, Leo J de Koning, Luitzen de Jong, Chris G de Koster,

The Gel/Gas/Phr family of fungal beta(1,3)-glucanosyltransferases plays an important role in cell wall biogenesis by processing the main component beta(1,3)-glucan. Two subfamilies are distinguished depending on the presence or absence of a C-terminal cysteine-rich domain, denoted "Cys-box." The N-terminal domain (NtD) contains the catalytic residues for transglycosidase activity and is ... Read more >>

J Biol Chem (The Journal of biological chemistry)
[2008, 283(27):18553-18565]

Cited: 22 times

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Expression of the alpha3/beta1 isoform of human Na,K-ATPase in the methylotrophic yeast Pichia pastoris.

Cristina Reina, Gloria Padoani, Cristina Carotti, Annamaria Merico, Grazia Tripodi, Patrizia Ferrari, Laura Popolo,

Na,K-ATPase is a crucial enzyme for ion homeostasis in human tissues. Different isozymes are produced by assembly of four alpha- and three beta-subunits. The expression of the alpha3/beta1 isozyme is confined to brain and heart. Its heterologous production has so far never been attempted in a lower eukaryote. In this ... Read more >>

FEMS Yeast Res (FEMS yeast research)
[2007, 7(4):585-594]

Cited: 1 time

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