Full Text Journal Articles by
Author L B Larsen

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Effect of protein concentrate mixtures and dietary addition of exogenous phytase on major milk minerals and proteins, including casein phosphorylation.

N A Poulsen, G Giagnoni, M Johansen, P Lund, L B Larsen,

Variations in major milk minerals, proteins, and their posttranslational modifications are largely under genetic influence, whereas the effect of nongenetic factors is less studied. Through a controlled feeding experiment (incomplete balanced Latin square design), the effect of concentrate mixtures, based on fava beans, rapeseed meal, or soybean meal as main ... Read more >>

J Dairy Sci (Journal of dairy science)
[2021, :]

Cited: 0 times

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Genetic analysis on infrared-predicted milk minerals for Danish dairy cattle.

R M Zaalberg, N A Poulsen, H Bovenhuis, J Sehested, L B Larsen, A J Buitenhuis,

A group of milk components that has shown potential to be predicted with milk spectra is milk minerals. Milk minerals are important for human health and cow health. Having an inexpensive and fast way to measure milk mineral concentrations would open doors for research, herd management, and selective breeding. The ... Read more >>

J Dairy Sci (Journal of dairy science)
[2021, 104(8):8947-8958]

Cited: 0 times

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Natural variations of citrate and calcium in milk and their effects on milk processing properties.

M Akkerman, L B Larsen, J Sørensen, N A Poulsen,

Natural variations among milk constituents, and their relations to each other as well as to processing parameters, represent possibilities for differentiation of milk to produce high-quality natural products. In this study, we focused on natural variations in milk citrate and its interplay with calcium distribution in milk, in relation to ... Read more >>

J Dairy Sci (Journal of dairy science)
[2019, 102(8):6830-6841]

Cited: 1 time

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Review: extended lactation in dairy cattle.

J Sehested, C Gaillard, J O Lehmann, G M Maciel, M Vestergaard, M R Weisbjerg, L Mogensen, L B Larsen, N A Poulsen, T Kristensen,

This paper reviews the effects of extended lactation (EXT) as a strategy in dairy cattle on milk production and persistency, reproduction, milk quality, lifetime performance of the cow and finally the economic effects on herd and farm levels as well as the impact on emission of greenhouse gas at product ... Read more >>

Animal (Animal : an international journal of animal bioscience)
[2019, 13(S1):s65-s74]

Cited: 2 times

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The effect of UHT-processed dairy milk on cardio-metabolic risk factors.

C K Hansen, L Klingenberg, L B Larsen, J K Lorenzen, K V Sørensen, A Astrup,

<h4>Background/objectives</h4>Cardiovascular disease (CVD) remains a major cause of death worldwide. Whereas dairy generally is associated with a neutral or a beneficial CVD effect, the consumption of ultra-high temperature (UHT)-treated milk has been reported to increase levels of low-density lipoprotein cholesterol (LDL-C) in an uncontrolled study. Our aim was to examine ... Read more >>

Eur J Clin Nutr (European journal of clinical nutrition)
[2017, 71(12):1463-1466]

Cited: 1 time

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Good sensory quality and cheesemaking properties in milk from Holstein cows managed for an 18-month calving interval.

G M Maciel, N A Poulsen, M K Larsen, U Kidmose, C Gaillard, J Sehested, L B Larsen,

Extended calving interval (CInt) for high-yielding dairy cows beyond the traditional 12mo has been suggested as a profitable, environmentally and welfare-friendly production strategy. However, concerns exist on whether extending cow CInt, and consequently prolonging lactation length, impairs milk quality. The aim of this study was to compare the quality of ... Read more >>

J Dairy Sci (Journal of dairy science)
[2016, 99(11):8524-8536]

Cited: 3 times

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Quantification of bovine milk protein composition and coagulation properties using infrared spectroscopy and chemometrics: A result of collinearity among reference variables.

C E Eskildsen, T Skov, M S Hansen, L B Larsen, N A Poulsen,

Predicting protein fractions and coagulation properties in bovine milk using Fourier transform infrared (FT-IR) measurements is desirable. However, such predictions may rely on correlations with total protein content. The aim of this study was to show how correlations between total protein content, protein fractions, and coagulation properties are responsible for ... Read more >>

J Dairy Sci (Journal of dairy science)
[2016, 99(10):8178-8186]

Cited: 3 times

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Effects of the diacylglycerol o-acyltransferase 1 (DGAT1) K232A polymorphism on fatty acid, protein, and mineral composition of dairy cattle milk.

H Bovenhuis, M H P W Visker, N A Poulsen, J Sehested, H J F van Valenberg, J A M van Arendonk, L B Larsen, A J Buitenhuis,

Several studies have described associations between the diacylglycerol o-acyltransferase 1 (DGAT1) K232A polymorphism and routinely collected milk production traits but not much is known about effects of the DGAT1 polymorphism on detailed milk composition. The aim of this study was to estimate effects of the DGAT1 polymorphism on milk fatty ... Read more >>

J Dairy Sci (Journal of dairy science)
[2016, 99(4):3113-3123]

Cited: 13 times

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Detection of genetic variation affecting milk coagulation properties in Danish Holstein dairy cattle by analyses of pooled whole-genome sequences from phenotypically extreme samples (pool-seq).

H P Bertelsen, V R Gregersen, N Poulsen, R O Nielsen, A Das, L B Madsen, A J Buitenhuis, L-E Holm, F Panitz, L B Larsen, C Bendixen,

Rennet-induced milk coagulation is an important trait for cheese production. Recent studies have reported an alarming frequency of cows producing poorly coagulating milk unsuitable for cheese production. Several genetic factors are known to affect milk coagulation, including variation in the major milk proteins; however, recent association studies indicate genetic effects ... Read more >>

J Anim Sci (Journal of animal science)
[2016, 94(4):1365-1376]

Cited: 1 time

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Factors influencing degree of glycosylation and phosphorylation of caseins in individual cow milk samples.

N A Poulsen, H B Jensen, L B Larsen,

The aim of this study was to examine variations in posttranslational modifications (PTM) of caseins (CN) in milk from individual cows and determine how these differ between breeds, across lactation, and between variants. Furthermore, we examined the variation of casein PTM in relation to rennet coagulation properties of milk. In ... Read more >>

J Dairy Sci (Journal of dairy science)
[2016, 99(5):3325-3333]

Cited: 8 times

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Effect of heating strategies on whey protein denaturation--Revisited by liquid chromatography quadrupole time-of-flight mass spectrometry.

M Akkerman, V M Rauh, M Christensen, L B Johansen, M Hammershøj, L B Larsen,

Previous standards in the area of effect of heat treatment processes on milk protein denaturation were based primarily on laboratory-scale analysis and determination of denaturation degrees by, for example, electrophoresis. In this study, whey protein denaturation was revisited by pilot-scale heating strategies and liquid chromatography quadrupole time-of-flight mass spectrometer (LC/MC ... Read more >>

J Dairy Sci (Journal of dairy science)
[2016, 99(1):152-166]

Cited: 2 times

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Short communication: Multi-trait estimation of genetic parameters for milk protein composition in the Danish Holstein.

G Gebreyesus, M S Lund, L Janss, N A Poulsen, L B Larsen, H Bovenhuis, A J Buitenhuis,

Genetic parameters were estimated for the major milk proteins using bivariate and multi-trait models based on genomic relationships between animals. The analyses included, apart from total protein percentage, αS1-casein (CN), αS2-CN, β-CN, κ-CN, α-lactalbumin, and β-lactoglobulin, as well as the posttranslational sub-forms of glycosylated κ-CN and αS1-CN-8P (phosphorylated). Standard errors ... Read more >>

J Dairy Sci (Journal of dairy science)
[2016, 99(4):2863-2866]

Cited: 16 times

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An impedance method for spatial sensing of 3D cell constructs--towards applications in tissue engineering.

C Canali, C Mazzoni, L B Larsen, A Heiskanen, Ø G Martinsen, A Wolff, M Dufva, J Emnéus,

We present the characterisation and validation of multiplexed 4-terminal (4T) impedance measurements as a method for sensing the spatial location of cell aggregates within large three-dimensional (3D) gelatin scaffolds. The measurements were performed using an array of four rectangular chambers, each having eight platinum needle electrodes for parallel analysis. The ... Read more >>

Analyst (The Analyst)
[2015, 140(17):6079-6088]

Cited: 4 times

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Interdependence of initial cell density, drug concentration and exposure time revealed by real-time impedance spectroscopic cytotoxicity assay.

C Caviglia, K Zór, S Canepa, M Carminati, L B Larsen, R Raiteri, T L Andresen, A Heiskanen, J Emnéus,

We investigated the combined effect of the initial cell density (12,500, 35,000, 75,000, and 100,000 cells cm(-2)) and concentration of the anti-cancer drug doxorubicin on HeLa cells by performing time-dependent cytotoxicity assays using real-time electrochemical impedance spectroscopy. A correlation between the rate of cell death and the initial cell seeding ... Read more >>

Analyst (The Analyst)
[2015, 140(10):3623-3629]

Cited: 9 times

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Phenotypic and genetic associations of milk traits with milk coagulation properties.

N A Poulsen, A J Buitenhuis, L B Larsen,

The aim of this study was to examine milk composition and rennet-induced coagulation properties of milk from 892 individual Danish Holstein and Danish Jersey cows and determine the genetic influences on these properties by determining heritability and genomic correlations with single nucleotide polymorphisms identified by the bovine HD Beadchip (Illumina ... Read more >>

J Dairy Sci (Journal of dairy science)
[2015, 98(4):2079-2087]

Cited: 12 times

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Bovine chromosomal regions affecting rheological traits in rennet-induced skim milk gels.

V R Gregersen, F Gustavsson, M Glantz, O F Christensen, H Stålhammar, A Andrén, H Lindmark-Månsson, N A Poulsen, L B Larsen, M Paulsson, C Bendixen,

Optimizing cheese yield and quality is of central importance to cheese manufacturing. The yield is associated with the time it takes before the gel has an optimal consistency for further processing, and it is well known that gel formation differs between individual milk samples. By identifying genomic regions affecting traits ... Read more >>

J Dairy Sci (Journal of dairy science)
[2015, 98(2):1261-1272]

Cited: 11 times

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Quantification of individual fatty acids in bovine milk by infrared spectroscopy and chemometrics: understanding predictions of highly collinear reference variables.

C E Eskildsen, M A Rasmussen, S B Engelsen, L B Larsen, N A Poulsen, T Skov,

Predicting individual fatty acids (FA) in bovine milk from Fourier transform infrared (FT-IR) measurements is desirable. However, such predictions may rely on covariance structures among individual FA and total fat content. These covariance structures may change with factors such as breed and feed, among others. The aim of this study ... Read more >>

J Dairy Sci (Journal of dairy science)
[2014, 97(12):7940-7951]

Cited: 23 times

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In vitro digestion of purified β-casein variants A(1), A(2), B, and I: effects on antioxidant and angiotensin-converting enzyme inhibitory capacity.

B Petrat-Melin, P Andersen, J T Rasmussen, N A Poulsen, L B Larsen, J F Young,

Genetic polymorphisms of bovine milk proteins affect the protein profile of the milk and, hence, certain technological properties, such as casein (CN) number and cheese yield. However, reports show that such polymorphisms may also affect the health-related properties of milk. Therefore, to gain insight into their digestion pattern and bioactive ... Read more >>

J Dairy Sci (Journal of dairy science)
[2015, 98(1):15-26]

Cited: 17 times

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Genetic parameters for rennet- and acid-induced coagulation properties in milk from Swedish Red dairy cows.

F Gustavsson, M Glantz, N A Poulsen, L Wadsö, H Stålhammar, A Andrén, H Lindmark Månsson, L B Larsen, M Paulsson, W F Fikse,

Milk coagulation is an important processing trait, being the basis for production of both cheese and fermented products. There is interest in including technological properties of these products in the breeding goal for dairy cattle. The aim of the present study was therefore to estimate genetic parameters for milk coagulation ... Read more >>

J Dairy Sci (Journal of dairy science)
[2014, 97(8):5219-5229]

Cited: 5 times

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Effects of breed and casein genetic variants on protein profile in milk from Swedish Red, Danish Holstein, and Danish Jersey cows.

F Gustavsson, A J Buitenhuis, M Johansson, H P Bertelsen, M Glantz, N A Poulsen, H Lindmark Månsson, H Stålhammar, L B Larsen, C Bendixen, M Paulsson, A Andrén,

In selecting cows for higher milk yields and milk quality, it is important to understand how these traits are affected by the bovine genome. The major milk proteins exhibit genetic polymorphism and these genetic variants can serve as markers for milk composition, milk production traits, and technological properties of milk. ... Read more >>

J Dairy Sci (Journal of dairy science)
[2014, 97(6):3866-3877]

Cited: 27 times

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The occurrence of noncoagulating milk and the association of bovine milk coagulation properties with genetic variants of the caseins in 3 Scandinavian dairy breeds.

N A Poulsen, H P Bertelsen, H B Jensen, F Gustavsson, M Glantz, H Lindmark Månsson, A Andrén, M Paulsson, C Bendixen, A J Buitenhuis, L B Larsen,

Substantial variation in milk coagulation properties has been observed among dairy cows. Consequently, raw milk from individual cows and breeds exhibits distinct coagulation capacities that potentially affect the technological properties and milk processing into cheese. This variation is largely influenced by protein composition, which is in turn affected by underlying ... Read more >>

J Dairy Sci (Journal of dairy science)
[2013, 96(8):4830-4842]

Cited: 29 times

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Estimation of genetic parameters and detection of quantitative trait loci for metabolites in Danish Holstein milk.

A J Buitenhuis, U K Sundekilde, N A Poulsen, H C Bertram, L B Larsen, P Sørensen,

Small components and metabolites in milk are significant for the utilization of milk, not only in dairy food production but also as disease predictors in dairy cattle. This study focused on estimation of genetic parameters and detection of quantitative trait loci for metabolites in bovine milk. For this purpose, milk ... Read more >>

J Dairy Sci (Journal of dairy science)
[2013, 96(5):3285-3295]

Cited: 21 times

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Effect of milk hydrolysates on inflammation markers and drug-induced transcriptional alterations in cell-based models.

D S G Nielsen, P K Theil, L B Larsen, S Purup,

Nonsteroidal anti-inflammatory drugs (NSAID) are associated with gastrointestinal inflammation and subsequent damage to the intestinal tissue. Earlier studies in our laboratory have found that specific casein hydrolysates (CH) might be useful in the treatment of gastrointestinal wounds. The underlying mechanisms that support inflammation and wound healing are not completely understood, ... Read more >>

J Anim Sci (Journal of animal science)
[2012, 90 Suppl 4:403-405]

Cited: 11 times

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Nuclear magnetic resonance metabonomics reveals strong association between milk metabolites and somatic cell count in bovine milk.

U K Sundekilde, N A Poulsen, L B Larsen, H C Bertram,

Somatic cell count (SCC) is associated with changes in milk composition, including changes in proteins, lipids, and milk metabolites. Somatic cell count is normally used as an indicator of mastitis infection. The compositional changes in protein and fat affect milk coagulation properties, and also the metabolite composition is thought to ... Read more >>

J Dairy Sci (Journal of dairy science)
[2013, 96(1):290-299]

Cited: 32 times

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Meat Science and Muscle Biology Symposium: in utero nutrition related to fetal development, postnatal performance, and meat quality of pork.

N Oksbjerg, P M Nissen, M Therkildsen, H S Møller, L B Larsen, M Andersen, J F Young,

Intrauterine growth restriction (IUGR) occurs naturally in pigs and leads to low birth weight of piglets due to undernutrition caused by placental insufficiency. For 2 main reasons, low birth weight causes economic loss. First, low birth weight pigs have a greater mortality and increasing the litter size causes more low ... Read more >>

J Anim Sci (Journal of animal science)
[2013, 91(3):1443-1453]

Cited: 18 times

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