Full Text Journal Articles by
Author Kelli L Hiett

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Draft Genome Sequences of Two Campylobacter jejuni Strains That Show Significantly Different Colonization Potentials in Chickens.

Kidon Sung, Bijay K Khajanchi, Kelli L Hiett, J Eric Line, Saeed A Khan,

Here, we report the draft genome sequences of robust (A74/C_24-3) and poor (A74/O_2-2) chicken-colonizing Campylobacter jejuni isolates. Whole-genome sequence analyses of these isolates will be helpful in facilitating further studies to identify genetic factors used in chicken colonization. ... Read more >>

Microbiol Resour Announc (Microbiology resource announcements)
[2020, 9(41):]

Cited: 0 times

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A loop-mediated isothermal amplification (LAMP) assay for the consensus detection of human pathogenic Campylobacter species.

Uma S Babu, Lisa M Harrison, Mark K Mammel, Elmer C Bigley, Kelli L Hiett, Kannan V Balan,

Most rapid identification methods for Campylobacter are designed to detect thermotolerant Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli). A growing number of thermosensitive Campylobacter species are now gaining recognition as emerging human pathogens. Methods are lacking for the rapid screening of these emerging species. Loop-mediated Isothermal Amplification (LAMP) ... Read more >>

J. Microbiol. Methods (Journal of microbiological methods)
[2020, 176:106009]

Cited: 0 times

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Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR.

Michael J Rothrock, Kristina M Feye, Sun Ae Kim, Si Hong Park, Aude Locatelli, Kelli L Hiett, John Gamble, Holly Sellers, Steven C Ricke,

Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathogens within a flock. The surface ... Read more >>

(Frontiers in microbiology)
[2018, 9:2454]

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Campylobacter jejuni Isolation/Enumeration from Environmental Samples.

Kelli L Hiett,

Currently, there is no universally accepted standard media or method for the recovery of Campylobacter species. This is likely due to the ubiquity of the organism in nature, the complex sample matrices from which the organism is often recovered, as well as the fragile/viable-but nonculturable state the organism assumes in ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2017, 1512:1-8]

Cited: 1 time

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In-Package Inactivation of Pathogenic and Spoilage Bacteria Associated with Poultry Using Dielectric Barrier Discharge-Cold Plasma Treatments.

Michael J Rothrock, Hong Zhuang, Kurt C Lawrence, Brian C Bowker, Gary R Gamble, Kelli L Hiett,

The goal of this study was to test the efficacy of in-package dielectric barrier discharge-cold plasma (DBD-CP) treatment to inactivate poultry-associated spoilage (Pseudomonas fluorescens) and pathogenic (Salmonella enterica Typhimurium, Campylobacter jejuni) bacteria. Liquid cultures of the bacterial isolates were sealed within packages containing ambient air (Trial 1) or modified air ... Read more >>

Curr. Microbiol. (Current microbiology)
[2017, 74(2):149-158]

Cited: 1 time

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Epitope mapping of Campylobacter jejuni flagellar capping protein (FliD) by chicken (Gallus gallus domesticus) sera.

Hung-Yueh Yeh, Arife Ezgi Telli, Jarra F Jagne, Christopher L Benson, Kelli L Hiett, John E Line,

Campylobacter jejuni, a Gram-negative rod, is a zoonotic pathogen associated with human acute bacterial gastroenteritis worldwide. The flagellum, composed of more than 35 proteins, is responsible for colonization of C. jejuni in the host gastrointestinal tract as well as inducing protective antibodies against the homologous serotype. In our previous study, ... Read more >>

Comp. Immunol. Microbiol. Infect. Dis. (Comparative immunology, microbiology and infectious diseases)
[2016, 49:76-81]

Cited: 0 times

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Antibiotic Resistance Patterns of Major Zoonotic Pathogens from All-Natural, Antibiotic-Free, Pasture-Raised Broiler Flocks in the Southeastern United States.

Michael J Rothrock, Kelli L Hiett, Jean Y Guard, Charlene R Jackson,

The use of antibiotics in agroecosystems has been implicated in the rise in antibiotic resistance (AR), which can affect environmental, animal, and human health. To determine the environmental impact of antibiotic use in agroecosystems, appropriate background levels of AR in agricultural environments in the absence of antibiotic application must be ... Read more >>

J. Environ. Qual. (Journal of environmental quality)
[2016, 45(2):593-603]

Cited: 11 times

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The characterization of Salmonella enterica serotypes isolated from the scalder tank water of a commercial poultry processing plant: Recovery of a multidrug-resistant Heidelberg strain.

Michael J Rothrock, Kimberly D Ingram, John Gamble, Jean Guard, Kellie M Cicconi-Hogan, Arthur Hinton, Kelli L Hiett,

The recent multistate outbreak of a multidrug-resistant (MDR) Salmonella Heidelberg strain from commercial poultry production highlights the need to better understand the reservoirs of these zoonotic pathogens within the commercial poultry production and processing environment. As part of a larger study looking at temporal changes in microbial communities within the ... Read more >>

Poult. Sci. (Poultry science)
[2015, 94(3):467-472]

Cited: 11 times

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Reactions of chicken sera to recombinant Campylobacter jejuni flagellar proteins.

Hung-Yueh Yeh, Kelli L Hiett, John E Line,

Campylobacter jejuni is a Gram-negative spiral rod bacterium and is the leading but underreported bacterial food-borne pathogen that causes human campylobacteriosis worldwide. Raw or undercooked poultry products are regarded as a major source for human infection. C. jejuni flagella have been implicated in colonization and adhesion to the mucosal surface ... Read more >>

Arch. Microbiol. (Archives of microbiology)
[2015, 197(2):353-358]

Cited: 5 times

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A hybrid DNA extraction method for the qualitative and quantitative assessment of bacterial communities from poultry production samples.

Michael J Rothrock, Kelli L Hiett, John Gamble, Andrew C Caudill, Kellie M Cicconi-Hogan, J Gregory Caporaso,

The efficacy of DNA extraction protocols can be highly dependent upon both the type of sample being investigated and the types of downstream analyses performed. Considering that the use of new bacterial community analysis techniques (e.g., microbiomics, metagenomics) is becoming more prevalent in the agricultural and environmental sciences and many ... Read more >>

J Vis Exp (Journal of visualized experiments : JoVE)
[2014, (94):]

Cited: 3 times

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Characterization and antigenicity of recombinant Campylobacter jejuni flagellar capping protein FliD.

Hung-Yueh Yeh, Kelli L Hiett, John E Line, Bruce S Seal,

Campylobacter jejuni, a flagellated, spiral-rod, Gram-negative bacterium, is the leading pathogen of human acute bacterial gastroenteritis worldwide, and chickens are regarded as a major reservoir of this micro-organism. Bacterial flagella, composed of more than 35 proteins, play important roles in colonization and adhesion to the mucosal surface of chicken caeca. ... Read more >>

J. Med. Microbiol. (Journal of medical microbiology)
[2014, 63(Pt 4):602-609]

Cited: 9 times

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Characterization and reactivity of broiler chicken sera to selected recombinant Campylobacter jejuni chemotactic proteins.

Hung-Yueh Yeh, Kelli L Hiett, John E Line, Bruce S Seal,

Campylobacter jejuni, a Gram-negative rod bacterium, is the leading causative agent of human acute bacterial gastroenteritis worldwide. Consumption and handling of raw or undercooked poultry are regarded as a major source for human infection. Because bacterial chemotaxis guides microorganisms to colonization and invasion in the host cells, proteins involved in ... Read more >>

Arch. Microbiol. (Archives of microbiology)
[2014, 196(5):375-383]

Cited: 2 times

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Characterization of the Campylobacter jejuni cryptic plasmid pTIW94 recovered from wild birds in the southeastern United States.

Kelli L Hiett, Michael J Rothrock, Bruce S Seal,

The complete nucleotide sequence was determined for a cryptic plasmid, pTIW94, recovered from several Campylobacter jejuni isolates from wild birds in the southeastern United States. pTIW94 is a circular molecule of 3860 nucleotides, with a G+C content (31.0%) similar to that of many Campylobacter spp. genomes. A typical origin of ... Read more >>

Plasmid (Plasmid)
[2013, 70(2):268-271]

Cited: 1 time

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Construction, expression, purification and antigenicity of recombinant Campylobacter jejuni flagellar proteins.

Hung-Yueh Yeh, Kelli L Hiett, John E Line, Brian B Oakley, Bruce S Seal,

Campylobacter jejuni, a flagellated, spiral-rod Gram-negative bacterium, is the leading etiologic agent of human acute bacterial gastroenteritis worldwide. The source of this microorganism for human infection has been implicated as consumption and handling of poultry meat where this microorganism is a commensal in the gut. Because the genomes of many ... Read more >>

Microbiol. Res. (Microbiological research)
[2013, 168(4):192-198]

Cited: 8 times

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The poultry-associated microbiome: network analysis and farm-to-fork characterizations.

Brian B Oakley, Cesar A Morales, J Line, Mark E Berrang, Richard J Meinersmann, Glenn E Tillman, Mark G Wise, Gregory R Siragusa, Kelli L Hiett, Bruce S Seal,

Microbial communities associated with agricultural animals are important for animal health, food safety, and public health. Here we combine high-throughput sequencing (HTS), quantitative-PCR assays, and network analysis to profile the poultry-associated microbiome and important pathogens at various stages of commercial poultry production from the farm to the consumer. Analysis of ... Read more >>

PLoS One (PloS one)
[2013, 8(2):e57190]

Cited: 36 times

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Pyrosequencing-based validation of a simple cell-suspension polymerase chain reaction assay for Campylobacter with application of high-processivity polymerase and novel internal amplification controls for rapid and specific detection.

Brian B Oakley, J Eric Line, Mark E Berrang, Jessica M Johnson, R Jeff Buhr, Nelson A Cox, Kelli L Hiett, Bruce S Seal,

Although Campylobacter is an important food-borne human pathogen, there remains a lack of molecular diagnostic assays that are simple to use, cost-effective, and provide rapid results in research, clinical, or regulatory laboratories. Of the numerous Campylobacter assays that do exist, to our knowledge none has been empirically tested for specificity ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2012, 72(2):131-138]

Cited: 5 times

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Application of high-throughput sequencing to measure the performance of commonly used selective cultivation methods for the foodborne pathogen Campylobacter.

Brian B Oakley, Cesar A Morales, J Eric Line, Bruce S Seal, Kelli L Hiett,

Campylobacter is an important foodborne human pathogen, which has traditionally been studied using a variety of selective cultivation methods. Here we use next-generation sequencing to ask the following: (i) how selective are commonly used Campylobacter cultivation methods relative to the initial sample and (ii) how do the specificity and sensitivity ... Read more >>

FEMS Microbiol. Ecol. (FEMS microbiology ecology)
[2012, 79(2):327-336]

Cited: 14 times

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Comparative genomics of four closely related Clostridium perfringens bacteriophages reveals variable evolution among core genes with therapeutic potential.

Brian B Oakley, Eldin Talundzic, Cesar A Morales, Kelli L Hiett, Gregory R Siragusa, Nikolay V Volozhantsev, Bruce S Seal,

BACKGROUND: Because biotechnological uses of bacteriophage gene products as alternatives to conventional antibiotics will require a thorough understanding of their genomic context, we sequenced and analyzed the genomes of four closely related phages isolated from Clostridium perfringens, an important agricultural and human pathogen. RESULTS: Phage whole-genome tetra-nucleotide signatures and proteomic ... Read more >>

BMC Genomics (BMC genomics)
[2011, 12(1):282]

Cited: 14 times

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Use of repetitive element palindromic PCR (rep-PCR) for the epidemiologic discrimination of foodborne pathogens.

Kelli L Hiett, Bruce S Seal,

The use of defined primers for polymerase chain reaction (PCR) amplification of interspersed repetitive DNA elements present at distinct locations in prokaryotic genomes is referred to as repetitive element sequence based-polymerase chain reaction (rep-PCR). The initial discovery of repetitive extragenic palindromic (REP) elements occurred in the genomes of Escherichia coli ... Read more >>

Methods Mol. Biol. (Methods in molecular biology (Clifton, N.J.))
[2009, 551:49-58]

Cited: 3 times

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Broiler Campylobacter contamination and human campylobacteriosis in Iceland.

Kenneth A Callicott, Hjördís Harğardóttir, Franklín Georgsson, Jarle Reiersen, Vala Friğriksdóttir, Eggert Gunnarsson, Pascal Michel, Jean-Robert Bisaillon, Karl G Kristinsson, Haraldur Briem, Kelli L Hiett, David S Needleman, Norman J Stern,

To examine whether there is a relationship between the degree of Campylobacter contamination observed in product lots of retail Icelandic broiler chicken carcasses and the incidence of human disease, 1,617 isolates from 327 individual product lots were genetically matched (using the flaA short variable region [SVR[) to 289 isolates from ... Read more >>

Appl. Environ. Microbiol. (Applied and environmental microbiology)
[2008, 74(21):6483-6494]

Cited: 20 times

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Genomic differences between Campylobacter jejuni isolates identify surface membrane and flagellar function gene products potentially important for colonizing the chicken intestine.

Kelli L Hiett, Alain Stintzi, Tracy M Andacht, Robin L Kuntz, Bruce S Seal,

Campylobacter spp. are one of the leading bacterial etiologic agents of acute human gastroenteritis among industrialized countries. Poultry are implicated as a major source of the organism for human illness; however, the factors involved with colonization of poultry gastrointestinal systems remain unclear. Genomics and proteomics analyses were used to identify ... Read more >>

Funct. Integr. Genomics (Functional & integrative genomics)
[2008, 8(4):407-420]

Cited: 22 times

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Proteomic analyses of a robust versus a poor chicken gastrointestinal colonizing isolate of Campylobacter jejuni.

Bruce S Seal, Kelli L Hiett, Robin L Kuntz, Rebekah Woolsey, Kathleen M Schegg, Mary Ard, Alain Stintzi,

Campylobacter spp. are a significant contributor to the bacterial etiology of acute gastroenteritis in humans. Epidemiological evidence implicates poultry as a major source of the organism for human illness. However, the factors involved in colonization of poultry with Campylobacter spp. remain unclear. Determining colonization-associated factors at the proteome level should ... Read more >>

J. Proteome Res. (Journal of proteome research)
[2007, 6(12):4582-4591]

Cited: 22 times

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Molecular phylogeny of the flaA short variable region among Campylobacter jejuni isolates collected during an annual evaluation of poultry flocks in the Southeastern United States.

Kelli L Hiett, Norman J Stern, Paula Fedorka-Cray, Nelson A Cox, Bruce S Seal,

Production and processing samples were collected from eight commercial poultry flocks in the southeastern United States and examined for the presence of Campylobacter spp. In an effort to determine relatedness, recovered isolates were typed using flaA short variable region (SVR) DNA sequence analysis. Six of the eight production flocks tested ... Read more >>

Foodborne Pathog. Dis. (Foodborne pathogens and disease)
[2007, 4(3):339-347]

Cited: 7 times

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Lack of evidence for vertical transmission of Campylobacter spp. in chickens.

Kenneth A Callicott, Vala Friethriksdóttir, Jarle Reiersen, Ruff Lowman, Jean-Robert Bisaillon, Eggert Gunnarsson, Eva Berndtson, Kelli L Hiett, David S Needleman, Norman J Stern,

Campylobacter jejuni is a major cause of bacterial food-borne infection in the industrial world. There is evidence that C. jejuni is present in eggs and hatchery fluff, opening the possibility for vertical transmission from hens to progeny. Poultry operations in Iceland provide an excellent opportunity to study this possibility, since ... Read more >>

Appl. Environ. Microbiol. (Applied and environmental microbiology)
[2006, 72(9):5794-5798]

Cited: 38 times

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Differentiation of campylobacter populations as demonstrated by flagellin short variable region sequences.

Richard J Meinersmann, Robert W Phillips, Kelli L Hiett, Paula Fedorka-Cray,

The DNA sequence of the flaA short variable region (SVR) was used to analyze a random population of Campylobacter isolates to investigate the weakly clonal population structure of members of the genus. The SVR sequence from 197 strains of C. jejuni and C. coli isolated from humans, bovine, swine, and ... Read more >>

Appl. Environ. Microbiol. (Applied and environmental microbiology)
[2005, 71(10):6368-6374]

Cited: 25 times

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