Full Text Journal Articles by
Author Kathleen A Thurman

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2173. Detection of Chlamydia psittaci by rtPCR in Outbreak Specimens Tested at CDC—2018.

Olivia L McGovern, Kelly Shaw, Christine Szablewski, Julie Gabel, Caroline Holsinger, Skyler Brennan, Bernard Wolff, Alvaro J Benitez, Maureen Diaz, Kathleen A Thurman, Jonas Winchell, Miwako Kobayashi,

AbstractBackgroundPsittacosis is a respiratory illness caused by Chlamydia psittaci. The most commonly available diagnostic tests are serologic tests, which have low sensitivity and can cross-react with other chlamydial species. Serologic tests also require paired sera collected weeks apart, which is impractical for patient management. Real-time polymerase chain reaction (rtPCR) testing ... Read more >>

Open Forum Infect Dis (Open forum infectious diseases)
[2019, 6(Suppl 2):S737-S738]

Cited: 0 times

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Improved Detection of Respiratory Pathogens by Use of High-Quality Sputum with TaqMan Array Card Technology.

Bernard J Wolff, Anna M Bramley, Kathleen A Thurman, Cynthia G Whitney, Brett Whitaker, Wesley H Self, Sandra R Arnold, Christopher Trabue, Richard G Wunderink, Jon McCullers, Kathryn M Edwards, Seema Jain, Jonas M Winchell,

New diagnostic platforms often use nasopharyngeal or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multipathogen testing to high-quality sputum specimens to determine if more pathogens can be identified relative to NP/OP swabs. Children (<18 years old) and adults hospitalized with CAP were ... Read more >>

J. Clin. Microbiol. (Journal of clinical microbiology)
[2017, 55(1):110-121]

Cited: 8 times

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Mycoplasma pneumoniae outbreak in a long-term care facility--Nebraska, 2014.

Deborah L Hastings, Kari J Harrington, Preeta K Kutty, Rebecca J Rayman, Dana Spindola, Maureen H Diaz, Kathleen A Thurman, Jonas M Winchell, Thomas J Safranek, ,

On June 20, 2014, a Nebraska long-term care facility notified the East Central District Health Department (ECDHD) and Nebraska Department of Health and Human Services (NDHHS) of an outbreak of respiratory illness characterized by cough and fever in 22 residents and resulting in four deaths during the preceding 2 weeks. ... Read more >>

MMWR Morb. Mortal. Wkly. Rep. (MMWR. Morbidity and mortality weekly report)
[2015, 64(11):296-299]

Cited: 9 times

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The multivariate detection limit for Mycoplasma pneumoniae as determined by nanorod array-surface enhanced Raman spectroscopy and comparison with limit of detection by qPCR.

Kelley C Henderson, Edward S Sheppard, Omar E Rivera-Betancourt, Joo-Young Choi, Richard A Dluhy, Kathleen A Thurman, Jonas M Winchell, Duncan C Krause,

Mycoplasma pneumoniae is a cell wall-less bacterial pathogen of the human respiratory tract that accounts for up to 20% of community-acquired pneumonia. At present, the standard for detection and genotyping is quantitative polymerase chain reaction (qPCR), which can exhibit excellent sensitivity but lacks standardization and has limited practicality for widespread, ... Read more >>

Analyst (The Analyst)
[2014, 139(24):6426-6434]

Cited: 9 times

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Survey of legionella species found in thai soil.

Tatiana C Travis, Ellen W Brown, Leonard F Peruski, Duangkamon Siludjai, Possawat Jorakate, Prasert Salika, Genyan Yang, Natalia A Kozak, Maja Kodani, Agnes K Warner, Claressa E Lucas, Kathleen A Thurman, Jonas M Winchell, Somsak Thamthitiwat, Barry S Fields,

Members of the Gram-negative genus Legionella are typically found in freshwater environments, with the exception of L. longbeachae, which is present in composts and potting mixes. When contaminated aerosols are inhaled, legionellosis may result, typically as either the more serious pneumonia Legionnaires' disease or the less severe flu-like illness Pontiac ... Read more >>

Int J Microbiol (International journal of microbiology)
[2012, 2012:218791]

Cited: 9 times

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Comparison of real-time PCR and a microimmunofluorescence serological assay for detection of chlamydophila pneumoniae infection in an outbreak investigation.

Alvaro J Benitez, Kathleen A Thurman, Maureen H Diaz, Laura Conklin, Newton E Kendig, Jonas M Winchell,

We assessed the performance of a recently validated real-time PCR assay and a commercially available microimmunofluorescence serologic test for the detection of Chlamydophila pneumoniae infection during an outbreak. Evaluation of specimens from 137 individuals suggests that real-time PCR holds greater utility as a diagnostic tool for early C. pneumoniae detection. ... Read more >>

J. Clin. Microbiol. (Journal of clinical microbiology)
[2012, 50(1):151-153]

Cited: 7 times

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Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay.

Kathleen A Thurman, Agnes K Warner, Kelley C Cowart, Alvaro J Benitez, Jonas M Winchell,

A multiplex real-time PCR assay for the detection of Mycoplasma pneumoniae (MP181), Chlamydia (Chlamydophila) pneumoniae (CP-Arg), Legionella spp. (Pan-Leg), and the human RNase P (RNase P) gene was developed for rapid testing of atypical bacterial respiratory pathogens in clinical specimens. This method uses 4 distinct hydrolysis probes to detect 3 ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2011, 70(1):1-9]

Cited: 50 times

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Identification of P1 variants of Mycoplasma pneumoniae by use of high-resolution melt analysis.

Stephanie B Schwartz, Stephanie L Mitchell, Kathleen A Thurman, Bernard J Wolff, Jonas M Winchell,

Mycoplasma pneumoniae is a leading cause of community-acquired pneumonia. Although two genetically distinct types of M. pneumoniae are known, variants of each also exist. We used a real-time PCR high-resolution melt genotyping assay to identify clinical variants which may provide greater insight into the genetic distribution of M. pneumoniae strains. ... Read more >>

J. Clin. Microbiol. (Journal of clinical microbiology)
[2009, 47(12):4117-4120]

Cited: 18 times

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Comparison of nucleic acid extraction methods for the detection of Mycoplasma pneumoniae.

Kathleen A Thurman, Kelley C Cowart, Jonas M Winchell,

Four nucleic acid extraction procedures (2 automated and 2 manual) were compared for their efficiency at isolating Mycoplasma pneumoniae DNA. Oropharyngeal swabs from healthy volunteers were spiked with varying amounts of M. pneumoniae, extracted, and tested using real-time polymerase chain reaction. Our data indicate that both automated extraction methods consistently ... Read more >>

Diagn. Microbiol. Infect. Dis. (Diagnostic microbiology and infectious disease)
[2009, 65(4):435-438]

Cited: 3 times

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Evaluation of two real-time PCR chemistries for the detection of Chlamydophila pneumoniae in clinical specimens.

Stephanie L Mitchell, Sona Budhiraja, Kathleen A Thurman, W Lanier Thacker, Jonas M Winchell,

Chlamydophila pneumoniae is an atypical bacterial respiratory pathogen that is responsible for approximately 3-10% of community-acquired pneumonia cases. We report the evaluation of two distinct real-time PCR assays for rapid and specific detection of C. pneumoniae. We tested 401 clinical specimens, finding 5.7% positive, and confirmed a localized outbreak. ... Read more >>

Mol. Cell. Probes (Molecular and cellular probes)
[2009, 23(6):309-311]

Cited: 9 times

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Comparison of laboratory diagnostic procedures for detection of Mycoplasma pneumoniae in community outbreaks.

Kathleen A Thurman, Nicholas D Walter, Stephanie B Schwartz, Stephanie L Mitchell, Michael T Dillon, Andrew L Baughman, Meredith Deutscher, John P Fulton, Jon E Tongren, Lauri A Hicks, Jonas M Winchell,

BACKGROUND: Mycoplasma pneumoniae continues to be a significant cause of community-acquired pneumonia (CAP). A more definitive methodology for reliable detection of M. pneumoniae is needed to identify outbreaks and to prevent potentially fatal extrapulmonary complications. METHODS: We analyzed 2 outbreaks of CAP due to M. pneumoniae. Nasopharyngeal and/or oropharyngeal swab ... Read more >>

Clin. Infect. Dis. (Clinical infectious diseases : an official publication of the Infectious Diseases Society of America)
[2009, 48(9):1244-1249]

Cited: 49 times

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Community outbreak of Mycoplasma pneumoniae infection: school-based cluster of neurologic disease associated with household transmission of respiratory illness.

Nicholas D Walter, Gavin B Grant, Utpala Bandy, Nicole E Alexander, Jonas M Winchell, Hannah T Jordan, James J Sejvar, Lauri A Hicks, David R Gifford, Nicole T Alexander, Kathleen A Thurman, Stephanie B Schwartz, Penelope H Dennehy, Nino Khetsuriani, Barry S Fields, Michael T Dillon, Dean D Erdman, Cynthia G Whitney, Matthew R Moore,

BACKGROUND: We investigated an outbreak of severe neurologic disease and pneumonia that occurred among students at 4 schools in Rhode Island. METHODS: We identified cases of encephalitis, encephalomyelitis, and pneumonia that occurred among schoolchildren from 1 September 2006 through 9 February 2007, and we performed serologic tests, polymerase chain reaction ... Read more >>

J. Infect. Dis. (The Journal of infectious diseases)
[2008, 198(9):1365-1374]

Cited: 30 times

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Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.

Jonas M Winchell, Kathleen A Thurman, Stephanie L Mitchell, W Lanier Thacker, Barry S Fields,

We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase ... Read more >>

J. Clin. Microbiol. (Journal of clinical microbiology)
[2008, 46(9):3116-3118]

Cited: 64 times

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