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Author Karen Liljebjelke

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Genetic characterization of AmpC and extended-spectrum beta-lactamase (ESBL) phenotypes in Escherichia coli and Salmonella from Alberta poultry

Tam Tran, Sylvia Checkley, Niamh Caffrey, Rashed Cassis, Chunu Mainali, Sheryl Gow, Agnes Agunos, Karen Liljebjelke,

Abstract Horizontal gene transfer is an important mechanism which facilitates bacterial populations in overcoming antimicrobial treatment. In this study, a total of 120 Escherichia coli and 62 Salmonella enterica subsp. enterica isolates were isolated from poultry farms in Alberta. Fourteen serovars were identified among Salmonella isolates. Thirty one percent of ... Read more >>

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Draft Genome Sequences of 12 Histophilus somni Strains Isolated from Feedlot Cattle in Alberta, Canada.

Mohammad Mostafa Nazari, Krishna Bhatt, Neil Rawlyk, Andrew A Potter, Karen Liljebjelke,

Histophilus somni is a Gram-negative opportunistic pathogen associated with respiratory disease in cattle. Here, we report the draft genome sequences of 12 Histophilus somni strains isolated from feedlot cattle in Alberta, Canada, which were diagnosed with respiratory disease. ... Read more >>

Microbiol Resour Announc (Microbiology resource announcements)
[2019, 8(46):]

Cited: 0 times

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Integrative Conjugative Element ICEHs1 Encodes for Antimicrobial Resistance and Metal Tolerance in Histophilus somni.

Krishna Bhatt, Edouard Timsit, Neil Rawlyk, Andrew Potter, Karen Liljebjelke,

The objectives of this study were to determine antimicrobial resistance and metal tolerance, and identify associated genes and mobile genetic elements in clinical strains of Histophilus somni isolated from feedlot cattle in Alberta during years 2012-2016 (contemporary isolates, n = 63) and years 1980-1990 (historical isolates, n = 31). Comparison ... Read more >>

(Frontiers in veterinary science)
[2018, 5:153]

Cited: 5 times

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Diversity of Antimicrobial Resistance Phenotypes in Salmonella Isolated from Commercial Poultry Farms.

Karen A Liljebjelke, Charles L Hofacre, David G White, Sherry Ayers, Margie D Lee, John J Maurer,

Salmonella remains the leading cause of foodborne illness in the United States, and the dissemination of drug-resistant Salmonellae through the food chain has important implications for treatment failure of salmonellosis. We investigated the ecology of Salmonella in integrated broiler production in order to understand the flow of antibiotic susceptible and ... Read more >>

(Frontiers in veterinary science)
[2017, 4:96]

Cited: 6 times

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Genome Sequences of Two Salmonella enterica Serovar Kentucky Isolates Recovered from Poultry Carcasses in the United States.

Bradd J Haley, Seon Woo Kim, Karen Liljebjelke, Jean Guard, Jo Ann S Van Kessel,

We report here the draft genome sequences of two Salmonella enterica serovar Kentucky eBurstGroup 15 isolates collected from poultry carcasses in Georgia (USA). ... Read more >>

(Genome announcements)
[2016, 4(6):]

Cited: 1 time

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Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica.

Jean Guard, Roxana Sanchez-Ingunza, Cesar Morales, Tod Stewart, Karen Liljebjelke, Joann Van Kessel, Kim Ingram, Deana Jones, Charlene Jackson, Paula Fedorka-Cray, Jonathan Frye, Richard Gast, Arthur Hinton,

Two DNA-based methods were compared for the ability to assign serotype to 139 isolates of Salmonella enterica ssp. I. Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB. A DNA microarray hybridization method that assessed the presence ... Read more >>

FEMS Microbiol. Lett. (FEMS microbiology letters)
[2012, 337(1):61-72]

Cited: 16 times

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Evaluation of two quantitative PCR assays using Bacteroidales and mitochondrial DNA markers for tracking dog fecal contamination in waterbodies.

Dinah D Tambalo, Tyler Boa, Karen Liljebjelke, Christopher K Yost,

This study describes a comparative performance evaluation of two qPCR assays targeting a dog-associated Bacteroidales 16S rRNA genetic marker (CanBac-UCD) and a dog mitochondrial DNA (mtDNA) marker. The same fecal and environmental samples were assayed for the two markers thereby allowing direct comparison. A wide range of non-target species including, ... Read more >>

J. Microbiol. Methods (Journal of microbiological methods)
[2012, 91(3):459-467]

Cited: 8 times

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Cross reactive cellular immune responses in chickens previously exposed to low pathogenic avian influenza.

Darrell R Kapczynski, Karen Liljebjelke, Gururaj Kulkarni, Henry Hunt, Hai Jun Jiang, Daniel Petkov,

BACKGROUND:Avian influenza (AI) infection in poultry can result in high morbidity and mortality, and negatively affect international trade. Because most AI vaccines used for poultry are inactivated, our knowledge of immunity against AI is based largely on humoral immune responses. In fact, little is known about cellular immunity following a ... Read more >>

(BMC proceedings)
[2011, 5 Suppl 4:S13]

Cited: 5 times

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Mucosal vaccination with a codon-optimized hemagglutinin gene expressed by attenuated Salmonella elicits a protective immune response in chickens against highly pathogenic avian influenza.

Karen A Liljebjelke, Daniel I Petkov, Darrell R Kapczynski,

The purpose of this study was to evaluate clinical protection from challenge conferred by two attenuated Salmonella enteria serovar typhimurium vaccine strains expressing the hemagglutinin (HA1) gene from a highly pathogenic avian influenza (HPAI) H5N1 (A/whooper swan/Mongolia/3/2005), under control of the anaerobically inducible nir15 promoter. Two-week-old White Leghorn chickens were ... Read more >>

Vaccine (Vaccine)
[2010, 28(27):4430-4437]

Cited: 11 times

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Vaccine-induced protection from egg production losses in commercial turkey breeder hens following experimental challenge with a triple-reassortant H3N2 avian influenza virus.

Darrell R Kapczynski, Eric Gonder, Karen Liljebjelke, Ron Lippert, Daniel Petkov, Becky Tilley,

Infections of avian influenza virus (AIV) in turkey breeder hens can cause a decrease in both egg production and quality, resulting in significant production losses. In North Carolina in 2003, a triple-reassortant H3N2 AIV containing human, swine, and avian gene segments was isolated from turkey breeder hens (A/turkey/NC/16108/03). This viral ... Read more >>

Avian Dis. (Avian diseases)
[2009, 53(1):7-15]

Cited: 6 times

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Investigation and control of an outbreak of salmonellosis caused by multidrug-resistant Salmonella typhimurium in a population of hospitalized horses.

Michael P Ward, Timea H Brady, Laurent L Cou√ętil, Karen Liljebjelke, John J Maurer, Ching Ching Wu,

An outbreak of salmonellosis in a population of hospitalized horses resulted in the closure of a teaching hospital for a period of 10 weeks. Fecal samples were collected from suspected cases and cultured for Salmonella. Salmonella isolates were characterized using antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE) and phage typing. ... Read more >>

Vet. Microbiol. (Veterinary microbiology)
[2005, 107(3-4):233-240]

Cited: 25 times

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Vertical and horizontal transmission of salmonella within integrated broiler production system.

Karen A Liljebjelke, Charles L Hofacre, Tongrui Liu, David G White, Sherry Ayers, Suzanne Young, John J Maurer,

Salmonella remains one of the leading causes of food-borne illness in the United States, and many key questions regarding the introduction and persistence in animal production systems still remain. In order to understand the ecology of Salmonella within an integrated commercial broiler production system, 289 Salmonella enterica were recovered from ... Read more >>

Foodborne Pathog. Dis. (Foodborne pathogens and disease)
[2005, 2(1):90-102]

Cited: 52 times

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Application of nested polymerase chain reaction to detection of Salmonella in poultry environment.

Tongrui Liu, Karen Liljebjelke, Elizabeth Bartlett, Charles Hofacre, Susan Sanchez, John J Maurer,

Isolation of Salmonella from environmental and processing-plant poultry samples requires the sampling of large numbers of areas within the poultry house or plant. Subsequently, the required number of samples necessitates a large volume of work for a microbiology laboratory, especially when the protocol requires the inclusion of a delayed secondary ... Read more >>

J. Food Prot. (Journal of food protection)
[2002, 65(8):1227-1232]

Cited: 20 times

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Duodenal obstruction caused by infection with Pythium insidiosum in a 12-week-old puppy.

Karen A Liljebjelke, Carley Abramson, Charles Brockus, Craig E Greene,

Pythium insidiosum is an aquatic fungus-like organism that causes a serious chronic granulomatous disease called pythiosis in animals and humans in tropical and subtropical regions of the world. In North America, pythiosis is most often diagnosed in the Gulf Coast states. Early recognition of the disease is crucial to successful ... Read more >>

J. Am. Vet. Med. Assoc. (Journal of the American Veterinary Medical Association)
[2002, 220(8):1188-91, 1162]

Cited: 5 times

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