Full Text Journal Articles by
Author Juan J Lasarte


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Genetic Modification of CD8+ T Cells to Express EGFR: Potential Application for Adoptive T Cell Therapies.

Teresa Lozano, Silvia Chocarro, Celia Martin, Aritz Lasarte-Cia, Cynthia Del Valle, Marta Gorraiz, Patricia Sarrión, Marina Ruiz de Galarreta, Amaia Lujambio, Sandra Hervás-Stubbs, Pablo Sarobe, Noelia Casares, Juan J Lasarte,

Adoptive immunotherapy with ex vivo-expanded tumor-infiltrating lymphocytes (TILs) has achieved objective clinical responses in a significant number of patients with cancer. The failure of many patients to develop long-term tumor control may be, in part, due to exhaustion of transferred T cells in the presence of a hostile tumor microenvironment. ... Read more >>

Front Immunol (Frontiers in immunology)
[2019, 10:2990]

Cited: 0 times

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FOXP3 Inhibitory Peptide P60 Increases Efficacy of Cytokine-induced Killer Cells Against Renal and Pancreatic Cancer Cells.

Maria Fitria Setiawan, Oliver Rudan, Annabelle Vogt, Maria A Gonzalez-Carmona, Bettina Langhans, Roland Schmidt-Wolf, Francesca Garofano, Christian P Strassburg, Juan J Lasarte, Noelia Casares, Teresa Lozano, Hans Weiher, Ingo G H Schmidt-Wolf,

BACKGROUND/AIM:Cytokine-induced killer (CIK) cells are ex vivo expanded major histocompatibility complex (MHC)-unrestricted cytotoxic cells with promising effects against a variety of cancer types. Regulatory T-cells (T-reg) have been shown to reduce the effectiveness of CIK cells against tumor cells. Peptide P60 has been shown to inhibit the immunosuppressive functions of ... Read more >>

Anticancer Res. (Anticancer research)
[2019, 39(10):5369-5374]

Cited: 0 times

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Short-Term Local Expression of a PD-L1 Blocking Antibody from a Self-Replicating RNA Vector Induces Potent Antitumor Responses.

Maria Cristina Ballesteros-Briones, Eva Martisova, Erkuden Casales, Noelia Silva-Pilipich, Maria Buñuales, Javier Galindo, Uxua Mancheño, Marta Gorraiz, Juan J Lasarte, Grazyna Kochan, David Escors, Alfonso R Sanchez-Paulete, Ignacio Melero, Jesus Prieto, Ruben Hernandez-Alcoceba, Sandra Hervas-Stubbs, Cristian Smerdou,

Immune checkpoint blockade has shown anti-cancer efficacy, but requires systemic administration of monoclonal antibodies (mAbs), often leading to adverse effects. To avoid toxicity, mAbs could be expressed locally in tumors. We developed adeno-associated virus (AAV) and Semliki Forest virus (SFV) vectors expressing anti-programmed death ligand 1 (aPDL1) mAb. When injected ... Read more >>

Mol. Ther. (Molecular therapy : the journal of the American Society of Gene Therapy)
[2019, 27(11):1892-1905]

Cited: 1 time

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Enhanced anti-tumor efficacy of checkpoint inhibitors in combination with the histone deacetylase inhibitor Belinostat in a murine hepatocellular carcinoma model.

Diana Llopiz, Marta Ruiz, Lorea Villanueva, Tamara Iglesias, Leyre Silva, Josune Egea, Juan J Lasarte, Perrine Pivette, Véronique Trochon-Joseph, Bérangère Vasseur, Graham Dixon, Bruno Sangro, Pablo Sarobe,

Immune checkpoint inhibitors are currently tested in different combinations in patients with advanced hepatocellular carcinoma (HCC). Nivolumab, an anti-PD-1 agent, has gained approval in the second-line setting in the USA. Epigenetic drugs have immune-mediated antitumor effects that may improve the activity of immunotherapy agents. Our aim was to study the ... Read more >>

Cancer Immunol. Immunother. (Cancer immunology, immunotherapy : CII)
[2019, 68(3):379-393]

Cited: 2 times

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A Combined PD-1/C5a Blockade Synergistically Protects against Lung Cancer Growth and Metastasis.

Daniel Ajona, Sergio Ortiz-Espinosa, Haritz Moreno, Teresa Lozano, María J Pajares, Jackeline Agorreta, Cristina Bértolo, Juan J Lasarte, Silvestre Vicent, Kai Hoehlig, Axel Vater, Fernando Lecanda, Luis M Montuenga, Ruben Pio,

Disruption of the programmed cell death protein 1 (PD-1) pathway with immune checkpoint inhibitors represents a major breakthrough in the treatment of non-small cell lung cancer. We hypothesized that combined inhibition of C5a/C5aR1 and PD-1 signaling may have a synergistic antitumor effect. The RMP1-14 antibody was used to block PD-1, ... Read more >>

Cancer Discov (Cancer discovery)
[2017, 7(7):694-703]

Cited: 32 times

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IL-10 expression defines an immunosuppressive dendritic cell population induced by antitumor therapeutic vaccination.

Diana Llopiz, Marta Ruiz, Stefany Infante, Lorea Villanueva, Leyre Silva, Sandra Hervas-Stubbs, Diego Alignani, Elizabeth Guruceaga, Juan J Lasarte, Pablo Sarobe,

Vaccination induces immunostimulatory signals that are often accompanied by regulatory mechanisms such as IL-10, which control T-cell activation and inhibit vaccine-dependent antitumor therapeutic effect. Here we characterized IL-10-producing cells in different tumor models treated with therapeutic vaccines. Although several cell subsets produced IL-10 irrespective of treatment, an early vaccine-dependent induction ... Read more >>

Oncotarget (Oncotarget)
[2017, 8(2):2659-2671]

Cited: 8 times

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Immune monitoring of immunosuppression withdrawal of liver transplant recipients.

Rocío García de la Garza, Pablo Sarobe, Juana Merino, Juan J Lasarte, Delia D'Avola, Virginia Belsue, José A Delgado, Leyre Silva, Mercedes Iñarrairaegui, Bruno Sangro, Iosu Sola, Fernando Pardo, Jorge Quiroga, J Ignacio Herrero,

Several studies have shown that some liver transplant recipients may tolerate immunosuppression withdrawal. Mechanisms and biomarkers of tolerance are not well known.Twenty-four LT patients with immunosuppression side-effects underwent progressive immunosuppression withdrawal. Peripheral lymphocyte populations and secretion of cytokines were analyzed at baseline and during withdrawal until tolerance (n = 15) ... Read more >>

Transpl. Immunol. (Transplant immunology)
[2015, 33(2):110-116]

Cited: 6 times

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Inhibition of FOXP3/NFAT Interaction Enhances T Cell Function after TCR Stimulation.

Teresa Lozano, Lorea Villanueva, Maika Durántez, Marta Gorraiz, Marta Ruiz, Virginia Belsúe, José I Riezu-Boj, Sandra Hervás-Stubbs, Julen Oyarzábal, Hozefa Bandukwala, Ana R Lourenço, Paul J Coffer, Pablo Sarobe, Jesús Prieto, Noelia Casares, Juan J Lasarte,

Regulatory T cell (Treg) activity is modulated by a cooperative complex between the transcription factor NFAT and FOXP3, a lineage specification factor for Tregs. FOXP3/NFAT interaction is required to repress expression of IL-2, upregulate expression of the Treg markers CTLA4 and CD25, and confer suppressor function to Tregs. However, FOXP3 ... Read more >>

J. Immunol. (Journal of immunology (Baltimore, Md. : 1950))
[2015, 195(7):3180-3189]

Cited: 10 times

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Genetic basis for clinical response to CTLA-4 blockade.

Ignacio Melero, Juan J Lasarte,

N. Engl. J. Med. (The New England journal of medicine)
[2015, 372(8):783]

Cited: 5 times

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Helper cell-independent antitumor activity of potent CD8(+) T cell epitope peptide vaccines is dependent upon CD40L.

Diana Llopiz, Eduardo Huarte, Marta Ruiz, Jaione Bezunartea, Virginia Belsúe, Aintzane Zabaleta, Juan J Lasarte, Jesús Prieto, Francisco Borrás-Cuesta, Pablo Sarobe,

Peptide vaccines derived from CD8(+) T-cell epitopes have shown variable efficacy in cancer patients. Thus, some peptide vaccines are capable of activating CD8(+) T-cell responses, even in the absence of CD4(+) T-cell epitopes or dendritic cell (DC)-activating adjuvants. However, the mechanisms underlying the clinical activity of these potent peptides are ... Read more >>

Oncoimmunology (Oncoimmunology)
[2013, 2(12):e27009]

Cited: 2 times

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Trial of complete weaning from immunosuppression for liver transplant recipients: factors predictive of tolerance.

Rocío García de la Garza, Pablo Sarobe, Juana Merino, Juan J Lasarte, Delia D'Avola, Virginia Belsue, José A Delgado, Leyre Silva, Mercedes Iñarrairaegui, Bruno Sangro, Jesus J Sola, Fernando Pardo, Jorge Quiroga, J Ignacio Herrero,

Recipients of liver transplantation (LT) may develop immunological tolerance. Factors predictive of tolerance are not clearly understood. Transplant recipients with normal liver function tests and without active viral hepatitis or autoimmune disease who presented with side effects of immunosuppression or a high risk of de novo malignancies were selected to ... Read more >>

Liver Transpl. (Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society)
[2013, 19(9):937-944]

Cited: 36 times

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Anaphylatoxin C5a creates a favorable microenvironment for lung cancer progression.

Leticia Corrales, Daniel Ajona, Stavros Rafail, Juan J Lasarte, Jose I Riezu-Boj, John D Lambris, Ana Rouzaut, Maria J Pajares, Luis M Montuenga, Ruben Pio,

The complement system contributes to various immune and inflammatory diseases, including cancer. In this study, we investigated the capacity of lung cancer cells to activate complement and characterized the consequences of complement activation on tumor progression. We focused our study on the production and role of the anaphylatoxin C5a, a ... Read more >>

J. Immunol. (Journal of immunology (Baltimore, Md. : 1950))
[2012, 189(9):4674-4683]

Cited: 95 times

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Tumor therapy in mice by using a tumor antigen linked to modulin peptides from Staphylococcus epidermidis.

Maika Durantez, Catherine Fayolle, Noelia Casares, Virginia Belsue, José I Riezu-Boj, Pablo Sarobe, Jesús Prieto, Francisco Borrás-Cuesta, Claude Leclerc, Juan J Lasarte,

Staphylococcus epidermidis releases a complex of at least four peptides, termed phenol-soluble modulins (PSM), which stimulate macrophages to produce proinflammatory cytokines via activation of TLR2 signalling pathway. We demonstrated that covalent linkage of PSM peptides to an antigen facilitate its capture by dendritic cells and, in combination with different TLR ... Read more >>

Vaccine (Vaccine)
[2010, 28(44):7146-7154]

Cited: 5 times

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Dynamic of nasal colonization by methicillin-resistant Staphylococcus aureus ST398 and ST1 after mupirocin treatment in a family in close contact with pigs.

Carmen Lozano, Carmen Aspiroz, Juan J Lasarte, Elena Gómez-Sanz, Myriam Zarazaga, Carmen Torres,

Nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA) was evaluated after a mupirocin treatment in a family previously colonized by MRSA sequence type ST398 and ST1, who lived close to a pig farm. Eight nasal samples were swabbed from each of the four family members on different moments after mupirocin treatment. ... Read more >>

Comp. Immunol. Microbiol. Infect. Dis. (Comparative Immunology, Microbiology and Infectious Diseases)
[2011, 34(1):e1-7]

Cited: 12 times

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Improved dendritic cell-based immunization against hepatitis C virus using peptide inhibitors of interleukin 10.

Nancy Díaz-Valdés, Lorea Manterola, Virginia Belsúe, José I Riezu-Boj, Esther Larrea, Itziar Echeverria, Diana Llópiz, Jacinto López-Sagaseta, Hervé Lerat, Jean-Michel Pawlotsky, Jesús Prieto, Juan J Lasarte, Francisco Borrás-Cuesta, Pablo Sarobe,

UNLABELLED: The high levels of interleukin 10 (IL-10) present in chronic hepatitis C virus (HCV) infection have been suggested as responsible for the poor antiviral cellular immune responses found in these patients. To overcome the immunosuppressive effect of IL-10 on antigen-presenting cells such as dendritic cells (DCs), we developed peptide ... Read more >>

Hepatology (Hepatology (Baltimore, Md.))
[2011, 53(1):23-31]

Cited: 14 times

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Skin lesion caused by ST398 and ST1 MRSA, Spain.

Carmen Aspiroz, Carmen Lozano, Ana Vindel, Juan J Lasarte, Myriam Zarazaga, Carmen Torres,

Emerging Infect. Dis. (Emerging infectious diseases)
[2010, 16(1):157-159]

Cited: 18 times

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The combined actions of NK and T lymphocytes are necessary to reject an EGFP+ mesenchymal tumor through mechanisms dependent on NKG2D and IFN gamma.

Ainhoa Arina, Oihana Murillo, Sandra Hervás-Stubbs, Arantza Azpilikueta, Juan Dubrot, Iñigo Tirapu, Eduardo Huarte, Carlos Alfaro, Jose L Pérez-Gracia, Gloria González-Aseguinolaza, Pablo Sarobe, Juan J Lasarte, Amanda Jamieson, Jesús Prieto, David H Raulet, Ignacio Melero,

Better understanding of the mechanisms that mediate spontaneous immune rejections ought to be important in the quest for improvements in immunotherapy of cancer. A set of intraperitoneal tumors of mesenchymal origin that had been chemically induced in ubiquitously expressing EGFP transgenic mice provided a model in which both T and ... Read more >>

Int. J. Cancer (International journal of cancer)
[2007, 121(6):1282-1295]

Cited: 13 times

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Identification of peptide inhibitors of transforming growth factor beta 1 using a phage-displayed peptide library.

Javier Dotor, Ana B López-Vázquez, Juan J Lasarte, Pablo Sarobe, Marta García-Granero, José I Riezu-Boj, Alfonso Martínez, Esperanza Feijoó, Jacinto López-Sagaseta, José Hermida, Jesús Prieto, Francisco Borrás-Cuesta,

Pathologies such as liver fibrosis and scleroderma are characterized by harmful levels of transforming growth factor beta 1 (TGFbeta1). These levels could be neutralized if inhibitors of this cytokine were available. With this aim we searched for peptides with binding affinity for TGFbeta1 using a phage-displayed random 15-mer peptide library. ... Read more >>

Cytokine (Cytokine)
[2007, 39(2):106-115]

Cited: 35 times

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Combined immunization with adjuvant molecules poly(I:C) and anti-CD40 plus a tumor antigen has potent prophylactic and therapeutic antitumor effects.

Diana Llopiz, Javier Dotor, Aintzane Zabaleta, Juan J Lasarte, Jesús Prieto, Francisco Borrás-Cuesta, Pablo Sarobe,

The low immunogenicity of malignant cells is one of the causes responsible for the lack of antitumor immune responses. Thus, development of new therapeutic strategies aimed at enhancing presentation of tumor antigens to T cells is a main goal of cancer immunotherapy. With this aim, we studied the efficacy of ... Read more >>

Cancer Immunol. Immunother. (Cancer immunology, immunotherapy : CII)
[2008, 57(1):19-29]

Cited: 21 times

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Vaccine-induced early control of hepatitis C virus infection in chimpanzees fails to impact on hepatic PD-1 and chronicity.

Christine S Rollier, Glaucia Paranhos-Baccala, Ernst J Verschoor, Babs E Verstrepen, Joost A R Drexhage, Zahra Fagrouch, Jean-Luc Berland, Florence Komurian-Pradel, Blandine Duverger, Nourredine Himoudi, Caroline Staib, Marcus Meyr, Mike Whelan, Joseph A Whelan, Victoria C Adams, Esther Larrea, José I Riezu, Juan J Lasarte, Birke Bartosch, Francois-L Cosset, Willy J M Spaan, Helmut M Diepolder, Gerd R Pape, Gerd Sutter, Genevieve Inchauspe, Jonathan L Heeney,

UNLABELLED: Broad T cell and B cell responses to multiple HCV antigens are observed early in individuals who control or clear HCV infection. The prevailing hypothesis has been that similar immune responses induced by prophylactic immunization would reduce acute virus replication and protect exposed individuals from chronic infection. Here, we ... Read more >>

Hepatology (Hepatology (Baltimore, Md.))
[2007, 45(3):602-613]

Cited: 60 times

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Induction of potent and long-lasting CD4 and CD8 T-cell responses against hepatitis C virus by immunization with viral antigens plus poly(I:C) and anti-CD40.

Aintzane Zabaleta, Laura Arribillaga, Diana Llopiz, Javier Dotor, Juan J Lasarte, Jesús Prieto, Francisco Borrás-Cuesta, Juan I Esteban, Josep Quer, Francesc Vayreda, Pablo Sarobe,

Development of vaccination strategies against hepatitis C virus (HCV) is of paramount importance. With this aim, we tested the ability of dendritic cell-activating reagents polyinosinic-polycytidylic acid (poly(I:C)) and anti-CD40, as adjuvants to induce T-cell responses against HCV. Immunization of mice with these adjuvants induced dendritic cell maturation in vivo. Also, ... Read more >>

Antiviral Res. (Antiviral research)
[2007, 74(1):25-35]

Cited: 9 times

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The extra domain A from fibronectin targets antigens to TLR4-expressing cells and induces cytotoxic T cell responses in vivo.

Juan J Lasarte, Noelia Casares, Marta Gorraiz, Sandra Hervás-Stubbs, Laura Arribillaga, Cristina Mansilla, Maika Durantez, Diana Llopiz, Pablo Sarobe, Francisco Borrás-Cuesta, Jesús Prieto, Claude Leclerc,

Vaccination strategies based on the in vivo targeting of Ags to dendritic cells (DCs) are needed to improve the induction of specific T cell immunity against tumors and infectious agents. In this study, we have used a recombinant protein encompassing the extra domain A from fibronectin (EDA), an endogenous ligand ... Read more >>

J. Immunol. (Journal of immunology (Baltimore, Md. : 1950))
[2007, 178(2):748-756]

Cited: 52 times

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Is plasma cardiotrophin-1 a marker of hypertensive heart disease?

Begoña López, Arantxa González, Juan J Lasarte, Pablo Sarobe, Francisco Borrás, Agnes Díaz, Joaquín Barba, Lourdes Tomás, Elisa Lozano, Manuel Serrano, Nerea Varo, Oscar Beloqui, María A Fortuño, Javier Díez,

OBJECTIVE:This study was designed to investigate whether plasma concentration of cardiotrophin-1 (CT-1), a cytokine that induces cardiomyocyte hypertrophy and stimulates cardiac fibroblasts, is related to hypertensive heart disease, as defined by the presence of echocardiographically assessed left ventricular hypertrophy (LVH). METHODS:The study was performed in 31 normotensive subjects and 111 ... Read more >>

J. Hypertens. (Journal of hypertension)
[2005, 23(3):625-632]

Cited: 43 times

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CD4+/CD25+ regulatory cells inhibit activation of tumor-primed CD4+ T cells with IFN-gamma-dependent antiangiogenic activity, as well as long-lasting tumor immunity elicited by peptide vaccination.

Noelia Casares, Laura Arribillaga, Pablo Sarobe, Javier Dotor, Ascensión Lopez-Diaz de Cerio, Ignacio Melero, Jesús Prieto, Francisco Borrás-Cuesta, Juan J Lasarte,

CD25(+) regulatory T (T reg) cells suppress the activation/proliferation of other CD4(+) or CD8(+) T cells in vitro. Also, down-regulation of CD25(+) T reg cells enhance antitumor immune responses. In this study, we show that depletion of CD25(+) T reg cells allows the host to induce both CD4(+) and CD8(+) ... Read more >>

J. Immunol. (Journal of immunology (Baltimore, Md. : 1950))
[2003, 171(11):5931-5939]

Cited: 105 times

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