Full Text Journal Articles by
Author Joseph D Gold

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Passive Stretch Induces Structural and Functional Maturation of Engineered Heart Muscle as Predicted by Computational Modeling.

Oscar J Abilez, Evangeline Tzatzalos, Huaxiao Yang, Ming-Tao Zhao, Gwanghyun Jung, Alexander M Zöllner, Malte Tiburcy, Johannes Riegler, Elena Matsa, Praveen Shukla, Yan Zhuge, Tony Chour, Vincent C Chen, Paul W Burridge, Ioannis Karakikes, Ellen Kuhl, Daniel Bernstein, Larry A Couture, Joseph D Gold, Wolfram H Zimmermann, Joseph C Wu,

The ability to differentiate human pluripotent stem cells (hPSCs) into cardiomyocytes (CMs) makes them an attractive source for repairing injured myocardium, disease modeling, and drug testing. Although current differentiation protocols yield hPSC-CMs to >90% efficiency, hPSC-CMs exhibit immature characteristics. With the goal of overcoming this limitation, we tested the effects ... Read more >>

Stem Cells (Stem cells (Dayton, Ohio))
[2018, 36(2):265-277]

Cited: 35 times

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Transcriptomic and epigenomic differences in human induced pluripotent stem cells generated from six reprogramming methods.

Jared M Churko, Jaecheol Lee, Mohamed Ameen, Mingxia Gu, Meenakshi Venkatasubramanian, Sebastian Diecke, Karim Sallam, Hogune Im, Gavin Wang, Joseph D Gold, Nathan Salomonis, Michael P Snyder, Joseph C Wu,

Many reprogramming methods can generate human induced pluripotent stem cells (hiPSCs) that closely resemble human embryonic stem cells (hESCs). This has led to assessments of how similar hiPSCs are to hESCs, by evaluating differences in gene expression, epigenetic marks and differentiation potential. However, all previous studies were performed using hiPSCs ... Read more >>

Nat Biomed Eng (Nature biomedical engineering)
[2017, 1(10):826-837]

Cited: 12 times

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Defined Engineered Human Myocardium With Advanced Maturation for Applications in Heart Failure Modeling and Repair.

Malte Tiburcy, James E Hudson, Paul Balfanz, Susanne Schlick, Tim Meyer, Mei-Ling Chang Liao, Elif Levent, Farah Raad, Sebastian Zeidler, Edgar Wingender, Johannes Riegler, Mouer Wang, Joseph D Gold, Izhak Kehat, Erich Wettwer, Ursula Ravens, Pieterjan Dierickx, Linda W van Laake, Marie Jose Goumans, Sara Khadjeh, Karl Toischer, Gerd Hasenfuss, Larry A Couture, Andreas Unger, Wolfgang A Linke, Toshiyuki Araki, Benjamin Neel, Gordon Keller, Lior Gepstein, Joseph C Wu, Wolfram-Hubertus Zimmermann,

<h4>Background</h4>Advancing structural and functional maturation of stem cell-derived cardiomyocytes remains a key challenge for applications in disease modeling, drug screening, and heart repair. Here, we sought to advance cardiomyocyte maturation in engineered human myocardium (EHM) toward an adult phenotype under defined conditions.<h4>Methods</h4>We systematically investigated cell composition, matrix, and media conditions ... Read more >>

Circulation (Circulation)
[2017, 135(19):1832-1847]

Cited: 144 times

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Patient-Specific iPSC-Derived Endothelial Cells Uncover Pathways that Protect against Pulmonary Hypertension in BMPR2 Mutation Carriers.

Mingxia Gu, Ning-Yi Shao, Silin Sa, Dan Li, Vittavat Termglinchan, Mohamed Ameen, Ioannis Karakikes, Gustavo Sosa, Fabian Grubert, Jaecheol Lee, Aiqin Cao, Shalina Taylor, Yu Ma, Zhixin Zhao, James Chappell, Rizwan Hamid, Eric D Austin, Joseph D Gold, Joseph C Wu, Michael P Snyder, Marlene Rabinovitch,

In familial pulmonary arterial hypertension (FPAH), the autosomal dominant disease-causing BMPR2 mutation is only 20% penetrant, suggesting that genetic variation provides modifiers that alleviate the disease. Here, we used comparison of induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) from three families with unaffected mutation carriers (UMCs), FPAH patients, and gender-matched controls ... Read more >>

Cell Stem Cell (Cell stem cell)
[2017, 20(4):490-504.e5]

Cited: 78 times

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Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas.

Johannes Riegler, Antje Ebert, Xulei Qin, Qi Shen, Mouer Wang, Mohamed Ameen, Kazuki Kodo, Sang-Ging Ong, Won Hee Lee, Grace Lee, Evgenios Neofytou, Joseph D Gold, Andrew J Connolly, Joseph C Wu,

The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected ... Read more >>

Stem Cell Reports (Stem cell reports)
[2016, 6(2):176-187]

Cited: 16 times

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Human Engineered Heart Muscles Engraft and Survive Long Term in a Rodent Myocardial Infarction Model.

Johannes Riegler, Malte Tiburcy, Antje Ebert, Evangeline Tzatzalos, Uwe Raaz, Oscar J Abilez, Qi Shen, Nigel G Kooreman, Evgenios Neofytou, Vincent C Chen, Mouer Wang, Tim Meyer, Philip S Tsao, Andrew J Connolly, Larry A Couture, Joseph D Gold, Wolfram H Zimmermann, Joseph C Wu,

<h4>Rationale</h4>Tissue engineering approaches may improve survival and functional benefits from human embryonic stem cell-derived cardiomyocyte transplantation, thereby potentially preventing dilative remodeling and progression to heart failure.<h4>Objective</h4>Assessment of transport stability, long-term survival, structural organization, functional benefits, and teratoma risk of engineered heart muscle (EHM) in a chronic myocardial infarction model.<h4>Methods and ... Read more >>

Circ Res (Circulation research)
[2015, 117(8):720-730]

Cited: 87 times

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Response to letter regarding article, "Cross talk of combined gene and cell therapy in ischemic heart disease: role of exosomal microRNA transfer".

Sang-Ging Ong, Won Hee Lee, Mei Huang, Devaveena Dey, Kazuki Kodo, Veronica Sanchez-Freire, Joseph D Gold, Joseph C Wu,

Circulation (Circulation)
[2015, 131(12):e385]

Cited: 1 time

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Epigenetic Regulation of Phosphodiesterases 2A and 3A Underlies Compromised β-Adrenergic Signaling in an iPSC Model of Dilated Cardiomyopathy.

Haodi Wu, Jaecheol Lee, Ludovic G Vincent, Qingtong Wang, Mingxia Gu, Feng Lan, Jared M Churko, Karim I Sallam, Elena Matsa, Arun Sharma, Joseph D Gold, Adam J Engler, Yang K Xiang, Donald M Bers, Joseph C Wu,

β-adrenergic signaling pathways mediate key aspects of cardiac function. Its dysregulation is associated with a range of cardiac diseases, including dilated cardiomyopathy (DCM). Previously, we established an iPSC model of familial DCM from patients with a mutation in TNNT2, a sarcomeric protein. Here, we found that the β-adrenergic agonist isoproterenol ... Read more >>

Cell Stem Cell (Cell stem cell)
[2015, 17(1):89-100]

Cited: 90 times

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Enabling consistency in pluripotent stem cell-derived products for research and development and clinical applications through material standards.

Anna French, Christopher Bravery, James Smith, Amit Chandra, Peter Archibald, Joseph D Gold, Natalie Artzi, Hae-Won Kim, Richard W Barker, Alexander Meissner, Joseph C Wu, Jonathan C Knowles, David Williams, Guillermo García-Cardeña, Doug Sipp, Steve Oh, Jeanne F Loring, Mahendra S Rao, Brock Reeve, Ivan Wall, Andrew J Carr, Kim Bure, Glyn Stacey, Jeffrey M Karp, Evan Y Snyder, David A Brindley,

There is a need for physical standards (reference materials) to ensure both reproducibility and consistency in the production of somatic cell types from human pluripotent stem cell (hPSC) sources. We have outlined the need for reference materials (RMs) in relation to the unique properties and concerns surrounding hPSC-derived products and ... Read more >>

Stem Cells Transl Med (Stem cells translational medicine)
[2015, 4(3):217-223]

Cited: 17 times

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Pravastatin reverses obesity-induced dysfunction of induced pluripotent stem cell-derived endothelial cells via a nitric oxide-dependent mechanism.

Mingxia Gu, Nicholas M Mordwinkin, Nigel G Kooreman, Jaecheol Lee, Haodi Wu, Shijun Hu, Jared M Churko, Sebastian Diecke, Paul W Burridge, Chunjiang He, Frances E Barron, Sang-Ging Ong, Joseph D Gold, Joseph C Wu,

<h4>Aims</h4>High-fat diet-induced obesity (DIO) is a major contributor to type II diabetes and micro- and macro-vascular complications leading to peripheral vascular disease (PVD). Metabolic abnormalities of induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) from obese individuals could potentially limit their therapeutic efficacy for PVD. The aim of this study was ... Read more >>

Eur Heart J (European heart journal)
[2015, 36(13):806-816]

Cited: 24 times

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Cardiac tissue slice transplantation as a model to assess tissue-engineered graft thickness, survival, and function.

Johannes Riegler, Astrid Gillich, Qi Shen, Joseph D Gold, Joseph C Wu,

<h4>Background</h4>Cell therapies offer the potential to improve cardiac function after myocardial infarction. Although injection of single-cell suspensions has proven safe, cell retention and survival rates are low. Tissue-engineered grafts allow cell delivery with minimal initial cell loss and mechanical support to the heart. However, graft performance cannot be easily compared, ... Read more >>

Circulation (Circulation)
[2014, 130(11 Suppl 1):S77-86]

Cited: 19 times

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Cross talk of combined gene and cell therapy in ischemic heart disease: role of exosomal microRNA transfer.

Sang-Ging Ong, Won Hee Lee, Mei Huang, Devaveena Dey, Kazuki Kodo, Veronica Sanchez-Freire, Joseph D Gold, Joseph C Wu,

<h4>Background</h4>Despite the promise shown by stem cells for restoration of cardiac function after myocardial infarction, the poor survival of transplanted cells has been a major issue. Hypoxia-inducible factor-1 (HIF1) is a transcription factor that mediates adaptive responses to ischemia. Here, we hypothesize that codelivery of cardiac progenitor cells (CPCs) with ... Read more >>

Circulation (Circulation)
[2014, 130(11 Suppl 1):S60-9]

Cited: 105 times

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Characterization of the molecular mechanisms underlying increased ischemic damage in the aldehyde dehydrogenase 2 genetic polymorphism using a human induced pluripotent stem cell model system.

Antje D Ebert, Kazuki Kodo, Ping Liang, Haodi Wu, Bruno C Huber, Johannes Riegler, Jared Churko, Jaecheol Lee, Patricia de Almeida, Feng Lan, Sebastian Diecke, Paul W Burridge, Joseph D Gold, Daria Mochly-Rosen, Joseph C Wu,

Nearly 8% of the human population carries an inactivating point mutation in the gene that encodes the cardioprotective enzyme aldehyde dehydrogenase 2 (ALDH2). This genetic polymorphism (ALDH2*2) is linked to more severe outcomes from ischemic heart damage and an increased risk of coronary artery disease (CAD), but the underlying molecular ... Read more >>

Sci Transl Med (Science translational medicine)
[2014, 6(255):255ra130]

Cited: 46 times

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Genome editing of isogenic human induced pluripotent stem cells recapitulates long QT phenotype for drug testing.

Yongming Wang, Ping Liang, Feng Lan, Haodi Wu, Leszek Lisowski, Mingxia Gu, Shijun Hu, Mark A Kay, Fyodor D Urnov, Rami Shinnawi, Joseph D Gold, Lior Gepstein, Joseph C Wu,

<h4>Background</h4>Human induced pluripotent stem cells (iPSCs) play an important role in disease modeling and drug testing. However, the current methods are time-consuming and lack an isogenic control.<h4>Objectives</h4>This study sought to establish an efficient technology to generate human PSC-based disease models with isogenic control.<h4>Methods</h4>The ion channel genes KCNQ1 and KCNH2 with ... Read more >>

J Am Coll Cardiol (Journal of the American College of Cardiology)
[2014, 64(5):451-459]

Cited: 75 times

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Electrical Integration of Human Embryonic Stem Cell-Derived Cardiomyocytes in a Guinea Pig Chronic Infarct Model.

Yuji Shiba, Dominic Filice, Sarah Fernandes, Elina Minami, Sarah K Dupras, Benjamin Van Biber, Peter Trinh, Yusuke Hirota, Joseph D Gold, Mohan Viswanathan, Michael A Laflamme,

<h4>Background</h4>Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) were recently shown to be capable of electromechanical integration following direct injection into intact or recently injured guinea pig hearts, and hESC-CM transplantation in recently injured hearts correlated with improvements in contractile function and a reduction in the incidence of arrhythmias. The present study ... Read more >>

J Cardiovasc Pharmacol Ther (Journal of cardiovascular pharmacology and therapeutics)
[2014, 19(4):368-381]

Cited: 34 times

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Costimulation-adhesion blockade is superior to cyclosporine A and prednisone immunosuppressive therapy for preventing rejection of differentiated human embryonic stem cells following transplantation.

Bruno C Huber, Julia D Ransohoff, Katherine J Ransohoff, Johannes Riegler, Antje Ebert, Kazuki Kodo, Yongquan Gong, Veronica Sanchez-Freire, Devaveena Dey, Nigel G Kooreman, Sebastian Diecke, Wendy Y Zhang, Justin Odegaard, Shijun Hu, Joseph D Gold, Robert C Robbins, Joseph C Wu,

<h4>Rationale</h4>Human embryonic stem cell (hESC) derivatives are attractive candidates for therapeutic use. The engraftment and survival of hESC derivatives as xenografts or allografts require effective immunosuppression to prevent immune cell infiltration and graft destruction.<h4>Objective</h4>To test the hypothesis that a short-course, dual-agent regimen of two costimulation-adhesion blockade agents can induce better ... Read more >>

Stem Cells (Stem cells (Dayton, Ohio))
[2013, 31(11):2354-2363]

Cited: 25 times

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Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts.

Yuji Shiba, Sarah Fernandes, Wei-Zhong Zhu, Dominic Filice, Veronica Muskheli, Jonathan Kim, Nathan J Palpant, Jay Gantz, Kara White Moyes, Hans Reinecke, Benjamin Van Biber, Todd Dardas, John L Mignone, Atsushi Izawa, Ramy Hanna, Mohan Viswanathan, Joseph D Gold, Michael I Kotlikoff, Narine Sarvazyan, Matthew W Kay, Charles E Murry, Michael A Laflamme,

Transplantation studies in mice and rats have shown that human embryonic-stem-cell-derived cardiomyocytes (hESC-CMs) can improve the function of infarcted hearts, but two critical issues related to their electrophysiological behaviour in vivo remain unresolved. First, the risk of arrhythmias following hESC-CM transplantation in injured hearts has not been determined. Second, the ... Read more >>

Nature (Nature)
[2012, 489(7415):322-325]

Cited: 343 times

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Returns of the living dead: therapeutic action of irradiated and mitotically inactivated embryonic stem cells.

Joseph D Gold, Joseph C Wu,

Circ Res (Circulation research)
[2012, 111(10):1250-1252]

Cited: 2 times

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Production of de novo cardiomyocytes: human pluripotent stem cell differentiation and direct reprogramming.

Paul W Burridge, Gordon Keller, Joseph D Gold, Joseph C Wu,

Cardiovascular disease is a leading cause of death worldwide. The limited capability of heart tissue to regenerate has prompted methodological developments for creating de novo cardiomyocytes, both in vitro and in vivo. Beyond uses in cell replacement therapy, patient-specific cardiomyocytes may find applications in drug testing, drug discovery, and disease ... Read more >>

Cell Stem Cell (Cell stem cell)
[2012, 10(1):16-28]

Cited: 331 times

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Neuregulin/ErbB signaling regulates cardiac subtype specification in differentiating human embryonic stem cells.

Wei-Zhong Zhu, Yiheng Xie, Kara White Moyes, Joseph D Gold, Bardia Askari, Michael A Laflamme,

<h4>Rationale</h4>Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) exhibit either a "working" chamber or a nodal-like phenotype. To generate optimal hESC-CM preparations for eventual clinical application in cell-based therapies, we will need to control their differentiation into these specialized cardiac subtypes.<h4>Objective</h4>To demonstrate intact neuregulin (NRG)-1β/ErbB signaling in hESC-CMs and test the hypothesis ... Read more >>

Circ Res (Circulation research)
[2010, 107(6):776-786]

Cited: 132 times

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Efficient generation and cryopreservation of cardiomyocytes derived from human embryonic stem cells.

Chunhui Xu, Shailaja Police, Mohammad Hassanipour, Yan Li, Yinhong Chen, Catherine Priest, Chris O'Sullivan, Michael A Laflamme, Wei-Zhong Zhu, Benjamin Van Biber, Livia Hegerova, Jiwei Yang, Karen Delavan-Boorsma, Anthony Davies, Jane Lebkowski, Joseph D Gold,

<h4>Aim</h4>Human embryonic stem cells (hESCs) represent a novel cell source to treat diseases such as heart failure and for use in drug screening. In this study, we aim to promote efficient generation of cardiomyocytes from hESCs by combining the current optimal techniques of controlled growth of undifferentiated cells and specific ... Read more >>

Regen Med (Regenerative medicine)
[2011, 6(1):53-66]

Cited: 54 times

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Human embryonic stem cell-derived cardiomyocytes can be maintained in defined medium without serum.

Chunhui Xu, Jia-Qiang He, Timothy J Kamp, Shailaja Police, Xiaoming Hao, Chris O'Sullivan, Melissa K Carpenter, Jane Lebkowski, Joseph D Gold,

Current procedures for the maintenance of cardiomyocytes from human embryonic stem (hES) cells rely on either co-culture with mouse cells or medium containing fetal bovine serum (FBS). Due to exposure to animal products, these methods carry the risk of potential pathogen contamination and increased immunogenicity. Additionally, FBS introduces inherent variability ... Read more >>

Stem Cells Dev (Stem cells and development)
[2006, 15(6):931-941]

Cited: 29 times

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Cardiac bodies: a novel culture method for enrichment of cardiomyocytes derived from human embryonic stem cells.

Chunhui Xu, Shailaja Police, Mohammad Hassanipour, Joseph D Gold,

Human embryonic stem (hES) cell-derived cardiomyocytes hold great promise for cardiovascular regenerative medicine. However, this application faces a number of challenges, including generating cardiomyocytes of adequate purity. With current protocols being used by several laboratories, cardiomyocyte differentiation from hES cells occurs at low frequency and results in a mixture of ... Read more >>

Stem Cells Dev (Stem cells and development)
[2006, 15(5):631-639]

Cited: 56 times

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Growth and differentiation of human embryonic stem cells for cardiac cell replacement therapy.

Chunhui Xu, Jane Lebkowski, Joseph D Gold,

Due to the limited proliferation capacity of cardiac cells, cell replacement therapy has been proposed to restore cardiac function in patients suffering from ischemic heart disease and congestive heart failure. However, this approach is challenged by an insufficient supply of appropriate cells. Because of their apparent indefinite replicative capacity and ... Read more >>

Curr Stem Cell Res Ther (Current stem cell research & therapy)
[2006, 1(2):173-187]

Cited: 4 times

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Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium.

Chunhui Xu, Elen Rosler, Jianjie Jiang, Jane S Lebkowski, Joseph D Gold, Chris O'Sullivan, Karen Delavan-Boorsma, Michael Mok, Adrienne Bronstein, Melissa K Carpenter,

Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor (bFGF), stem cell factor (SCF), and fetal liver tyrosine kinase-3 ... Read more >>

Stem Cells (Stem cells (Dayton, Ohio))
[2005, 23(3):315-323]

Cited: 205 times

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